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The map label for this gene is hupA

Identifier: 21674613

GI number: 21674613

Start: 1703861

End: 1704202

Strand: Reverse

Name: hupA

Synonym: CT1799

Alternate gene names: 21674613

Gene position: 1704202-1703861 (Counterclockwise)

Preceding gene: 21674616

Following gene: 21674612

Centisome position: 79.08

GC content: 61.99

Gene sequence:

>342_bases
ATGCACGAAATGTCCATTGCCATGTCGGTCGTCGAGGCCGTGGTTGACAAGGCCCGCGAGGAGGGCGGCGGCAAAATTAC
CGGTATCGATTTGGTTGTCGGACGGCTGGCTGGCGTGGAGGTCGAGTCGCTGAAATTCTGCTTCGGTGCGGCGGCGCGCG
GCACGCTGGCCGAAGGAGCCGAACTGGTGATCGAAGAGCCGGAGGGCCGTGGCCGCTGCGAAGCGTGCGGCGCGGAGTTT
CCTGTTACGAGTTTTTACGCGAAGTGTTCAGCGTGCGGGCAGTTCCGGGTGAAGATCGAATCTGGCCGGGAGCTTGCGGT
GCGTTCATTCACGATTGAGTGA

Upstream 100 bases:

>100_bases
ATCATTCATCACTGCCCCCAATCGTTGCAGAGGAACTGTTTTCGCCCGATTCGGGTACGTGGTGTCGAAAGTTCGTTTAT
CTGAAAATGACGGAAGGCCC

Downstream 100 bases:

>100_bases
AAACGGCTGCTCAACCATACTTGTAATTATACCATTCTGGGAGTAATCAACTATGTGCGATACTTGCGGTTGTTCAGGCG
ATGGCGGAGCGGTGCTGCGC

Product: hydrogenase expression/formation protein HupA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 113; Mature: 113

Protein sequence:

>113_residues
MHEMSIAMSVVEAVVDKAREEGGGKITGIDLVVGRLAGVEVESLKFCFGAAARGTLAEGAELVIEEPEGRGRCEACGAEF
PVTSFYAKCSACGQFRVKIESGRELAVRSFTIE

Sequences:

>Translated_113_residues
MHEMSIAMSVVEAVVDKAREEGGGKITGIDLVVGRLAGVEVESLKFCFGAAARGTLAEGAELVIEEPEGRGRCEACGAEF
PVTSFYAKCSACGQFRVKIESGRELAVRSFTIE
>Mature_113_residues
MHEMSIAMSVVEAVVDKAREEGGGKITGIDLVVGRLAGVEVESLKFCFGAAARGTLAEGAELVIEEPEGRGRCEACGAEF
PVTSFYAKCSACGQFRVKIESGRELAVRSFTIE

Specific function: Probably plays a role in an hydrogenase nickel cofactor insertion step

COG id: COG0375

COG function: function code R; Zn finger protein HypA/HybF (possibly regulating hydrogenase expression)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypA/hybF family

Homologues:

Organism=Escherichia coli, GI1789081, Length=114, Percent_Identity=31.5789473684211, Blast_Score=67, Evalue=3e-13,
Organism=Escherichia coli, GI1789365, Length=113, Percent_Identity=29.2035398230088, Blast_Score=66, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HYPA_CHLTE (Q8KBI8)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_662678.1
- GeneID:   1008274
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT1799
- NMPDR:   fig|194439.1.peg.1772
- TIGR:   CT1799
- HOGENOM:   HBG688671
- OMA:   MHEVSLM
- ProtClustDB:   CLSK571892
- BioCyc:   CTEP194439:CT_1799-MONOMER
- HAMAP:   MF_00213
- InterPro:   IPR000688
- InterPro:   IPR020538
- TIGRFAMs:   TIGR00100

Pfam domain/function: PF01155 HypA

EC number: NA

Molecular weight: Translated: 11975; Mature: 11975

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS01249 HYPA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
4.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHEMSIAMSVVEAVVDKAREEGGGKITGIDLVVGRLAGVEVESLKFCFGAAARGTLAEGA
CCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCC
ELVIEEPEGRGRCEACGAEFPVTSFYAKCSACGQFRVKIESGRELAVRSFTIE
EEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCEEEEEEEECC
>Mature Secondary Structure
MHEMSIAMSVVEAVVDKAREEGGGKITGIDLVVGRLAGVEVESLKFCFGAAARGTLAEGA
CCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCC
ELVIEEPEGRGRCEACGAEFPVTSFYAKCSACGQFRVKIESGRELAVRSFTIE
EEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901