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The map label for this gene is nirE [H]

Identifier: 21674577

GI number: 21674577

Start: 1672766

End: 1673668

Strand: Reverse

Name: nirE [H]

Synonym: CT1763

Alternate gene names: 21674577

Gene position: 1673668-1672766 (Counterclockwise)

Preceding gene: 21674584

Following gene: 21674576

Centisome position: 77.67

GC content: 62.02

Gene sequence:

>903_bases
ATGGTGAAGATGTCGCAAATTCTGACAAGAAAAGTACTTCACCTCTTTTTTTGTCAGGCGTTATATTTTTTAGGAAATAA
TGAAAGCCGTAAAGTGATTCATCAGATGGCGGAGCATGGAGAAAACAGGGTTCTGATTGTCGGCGCGGGGCCGGGCGATC
CGGAGCTGCTGACGGTGCGCGGCGCGGCGGCCATCCGCGAGGCCGATGTCATTCTCTACGACTGCGGGACGGTCGAGCCG
GTACTCGCGCTGGCATCCGAACGCGCCGCGATTGTTCGCGTGGATCGTTCGCCATATGAAACCGGCGAGGGGCGTCGCGA
ACAGACGCCGATGATCGTGGTGATTCGGGAGTATCGTGATCGTGGCTTGCGCGTCGTGCGGCTGAAAACCGGCGATCCGT
CGCTGTTCGGCGGCGAAGTTGATGAGGGCGACGTGCTCACGCGGCTCGGCATTCCGTGGGCCGCTATTCCCGGCATCTGT
GCCGGAGCGGCGGCGGCGAGCGCCTACGCCCTGCCGATCAGCCGCAAGTTCGAGAGCGACGCCGTGCTCAACCTCATCGC
CGCCGCGATCACCGACGACTTCGCGCTCATCCGCGATGCCGCACACTTGCTCGGCCATGGCGCGACGGTCGTGCTCTACA
TGGCGACGGCGAATCTGCCGGGTATCCTCCGGACTTTTCGTGAAGCGGGCGTGCCTGATGCAATGCCGGTCGTGGCGGTG
AGCAAGGCCGGATGGCCGGATGAGGCGTTTGCGCGGACGACTCTTGGCGAGCTAACGGCGGCTGGTTGTTCGATCGCCCT
GCCGGAGCCGGTGGTGTACCATATCGGGCGGTACGTGAGAGTTCGGAACGTTCCTGCGGGATCGCAAGAGTTTTTTGTCA
AGGCCCCCGAAAACGCCGGGTAG

Upstream 100 bases:

>100_bases
TCTTTTACGGCAATGGATGAGATTAAGAAACCTTAATTTTTTAATAAGAAAAGCTGTTTTCTGTAAATTTATATACCAAT
AGGTAGGAATTGTTTGAATA

Downstream 100 bases:

>100_bases
CCCCTTTTTTTGTACAGCATTATATATTAAGAGTTATAACGAAATCAACCATATCCATGAAGAAAGCAGTATTGATGGTG
TTGCTGCTGGCCGCGGTCAG

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MVKMSQILTRKVLHLFFCQALYFLGNNESRKVIHQMAEHGENRVLIVGAGPGDPELLTVRGAAAIREADVILYDCGTVEP
VLALASERAAIVRVDRSPYETGEGRREQTPMIVVIREYRDRGLRVVRLKTGDPSLFGGEVDEGDVLTRLGIPWAAIPGIC
AGAAAASAYALPISRKFESDAVLNLIAAAITDDFALIRDAAHLLGHGATVVLYMATANLPGILRTFREAGVPDAMPVVAV
SKAGWPDEAFARTTLGELTAAGCSIALPEPVVYHIGRYVRVRNVPAGSQEFFVKAPENAG

Sequences:

>Translated_300_residues
MVKMSQILTRKVLHLFFCQALYFLGNNESRKVIHQMAEHGENRVLIVGAGPGDPELLTVRGAAAIREADVILYDCGTVEP
VLALASERAAIVRVDRSPYETGEGRREQTPMIVVIREYRDRGLRVVRLKTGDPSLFGGEVDEGDVLTRLGIPWAAIPGIC
AGAAAASAYALPISRKFESDAVLNLIAAAITDDFALIRDAAHLLGHGATVVLYMATANLPGILRTFREAGVPDAMPVVAV
SKAGWPDEAFARTTLGELTAAGCSIALPEPVVYHIGRYVRVRNVPAGSQEFFVKAPENAG
>Mature_300_residues
MVKMSQILTRKVLHLFFCQALYFLGNNESRKVIHQMAEHGENRVLIVGAGPGDPELLTVRGAAAIREADVILYDCGTVEP
VLALASERAAIVRVDRSPYETGEGRREQTPMIVVIREYRDRGLRVVRLKTGDPSLFGGEVDEGDVLTRLGIPWAAIPGIC
AGAAAASAYALPISRKFESDAVLNLIAAAITDDFALIRDAAHLLGHGATVVLYMATANLPGILRTFREAGVPDAMPVVAV
SKAGWPDEAFARTTLGELTAAGCSIALPEPVVYHIGRYVRVRNVPAGSQEFFVKAPENAG

Specific function: Catalyzes the methylation of both C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin. Inactivation of uroporphyrinogen-III methyltran

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=268, Percent_Identity=30.5970149253731, Blast_Score=99, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6322922, Length=244, Percent_Identity=27.0491803278689, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 32199; Mature: 32199

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00839 SUMT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKMSQILTRKVLHLFFCQALYFLGNNESRKVIHQMAEHGENRVLIVGAGPGDPELLTVR
CCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEE
GAAAIREADVILYDCGTVEPVLALASERAAIVRVDRSPYETGEGRREQTPMIVVIREYRD
CCHHHEECCEEEEECCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEEHHH
RGLRVVRLKTGDPSLFGGEVDEGDVLTRLGIPWAAIPGICAGAAAASAYALPISRKFESD
CCEEEEEEECCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCEEECCCCCCCCHH
AVLNLIAAAITDDFALIRDAAHLLGHGATVVLYMATANLPGILRTFREAGVPDAMPVVAV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCEEEE
SKAGWPDEAFARTTLGELTAAGCSIALPEPVVYHIGRYVRVRNVPAGSQEFFVKAPENAG
ECCCCCCHHHHHHHHHHHHHCCCCEECCCHHHHHCCCEEEEECCCCCCCEEEEECCCCCC
>Mature Secondary Structure
MVKMSQILTRKVLHLFFCQALYFLGNNESRKVIHQMAEHGENRVLIVGAGPGDPELLTVR
CCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEE
GAAAIREADVILYDCGTVEPVLALASERAAIVRVDRSPYETGEGRREQTPMIVVIREYRD
CCHHHEECCEEEEECCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEEHHH
RGLRVVRLKTGDPSLFGGEVDEGDVLTRLGIPWAAIPGICAGAAAASAYALPISRKFESD
CCEEEEEEECCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCEEECCCCCCCCHH
AVLNLIAAAITDDFALIRDAAHLLGHGATVVLYMATANLPGILRTFREAGVPDAMPVVAV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCEEEE
SKAGWPDEAFARTTLGELTAAGCSIALPEPVVYHIGRYVRVRNVPAGSQEFFVKAPENAG
ECCCCCCHHHHHHHHHHHHHCCCCEECCCHHHHHCCCEEEEECCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7747927 [H]