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The map label for this gene is gcvH [H]

Identifier: 21674444

GI number: 21674444

Start: 1532919

End: 1533302

Strand: Reverse

Name: gcvH [H]

Synonym: CT1626

Alternate gene names: 21674444

Gene position: 1533302-1532919 (Counterclockwise)

Preceding gene: 21674445

Following gene: 21674443

Centisome position: 71.15

GC content: 54.69

Gene sequence:

>384_bases
ATGAACATCCCTGACAATCTCCGCTATACCAAAGATCACGAATGGATCAAGCTGCTTGAAGATGGCCTGACCGCTCTGGT
CGGCATCACCGACTTTGCGCAGTCCGAGCTTGGCGACATCGTGTTCGTCGAAACCAAACCGGTTGGAACAAAGGTTGCTG
CGCACGGTACGTTTGGAACCGTTGAGGCGGTCAAAACGGTTGCCGATCTGTTCGCTCCTGCGGCTGGCGAAATTGTCGAG
GTCAATGCTGGACTCGACGATGCGGCGATTGTTAACTCCGATCCCTACAACGAGGGCTGGATTGTCAAGATGAAGCTCGA
TAATCCTGCCGATGTCGAGGCACTACTCTCTCCGGCGGACTACAGCGCCCTGATCGGTGAGTAA

Upstream 100 bases:

>100_bases
GTCTGATTCGGTCGATCTTTTCTTCTCCTGAAAAAATGTTATTTTAAAGTTCATTTGTATTCCTTCCTCAATTGCATAGC
AACCTAAATCGCGTTCTGCC

Downstream 100 bases:

>100_bases
GATTTTCAACTGAATTTCAATACGCAACCATGCCTTTCATTGTCAATACCGACGCCGAGCGGGCCGAGATGCTGCGCGAA
ATCGGCGTCGAGAATTTTGA

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 127; Mature: 127

Protein sequence:

>127_residues
MNIPDNLRYTKDHEWIKLLEDGLTALVGITDFAQSELGDIVFVETKPVGTKVAAHGTFGTVEAVKTVADLFAPAAGEIVE
VNAGLDDAAIVNSDPYNEGWIVKMKLDNPADVEALLSPADYSALIGE

Sequences:

>Translated_127_residues
MNIPDNLRYTKDHEWIKLLEDGLTALVGITDFAQSELGDIVFVETKPVGTKVAAHGTFGTVEAVKTVADLFAPAAGEIVE
VNAGLDDAAIVNSDPYNEGWIVKMKLDNPADVEALLSPADYSALIGE
>Mature_127_residues
MNIPDNLRYTKDHEWIKLLEDGLTALVGITDFAQSELGDIVFVETKPVGTKVAAHGTFGTVEAVKTVADLFAPAAGEIVE
VNAGLDDAAIVNSDPYNEGWIVKMKLDNPADVEALLSPADYSALIGE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=119, Percent_Identity=38.655462184874, Blast_Score=100, Evalue=3e-22,
Organism=Homo sapiens, GI89057342, Length=119, Percent_Identity=37.8151260504202, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789271, Length=125, Percent_Identity=45.6, Blast_Score=109, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17507493, Length=117, Percent_Identity=39.3162393162393, Blast_Score=98, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17551294, Length=120, Percent_Identity=36.6666666666667, Blast_Score=91, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6319272, Length=122, Percent_Identity=41.8032786885246, Blast_Score=101, Evalue=3e-23,
Organism=Drosophila melanogaster, GI17865652, Length=120, Percent_Identity=38.3333333333333, Blast_Score=102, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 13543; Mature: 13543

Theoretical pI: Translated: 3.94; Mature: 3.94

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIPDNLRYTKDHEWIKLLEDGLTALVGITDFAQSELGDIVFVETKPVGTKVAAHGTFGT
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEECCCCHH
VEAVKTVADLFAPAAGEIVEVNAGLDDAAIVNSDPYNEGWIVKMKLDNPADVEALLSPAD
HHHHHHHHHHHCCCCCCEEEECCCCCCCEEECCCCCCCCEEEEEECCCCCCHHHHCCCCC
YSALIGE
CHHCCCC
>Mature Secondary Structure
MNIPDNLRYTKDHEWIKLLEDGLTALVGITDFAQSELGDIVFVETKPVGTKVAAHGTFGT
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEECCCCHH
VEAVKTVADLFAPAAGEIVEVNAGLDDAAIVNSDPYNEGWIVKMKLDNPADVEALLSPAD
HHHHHHHHHHHCCCCCCEEEECCCCCCCEEECCCCCCCCEEEEEECCCCCCHHHHCCCCC
YSALIGE
CHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA