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The map label for this gene is ispE

Identifier: 21674315

GI number: 21674315

Start: 1399308

End: 1400168

Strand: Reverse

Name: ispE

Synonym: CT1495

Alternate gene names: 21674315

Gene position: 1400168-1399308 (Counterclockwise)

Preceding gene: 21674318

Following gene: 21674312

Centisome position: 64.97

GC content: 59.93

Gene sequence:

>861_bases
ATGAAGCACTTTTCGGTCAAAGCCTGCGCCAAAATCAACCTCGGTCTGCTCATCACCAGCCGTCGGGCTGATGGCTATCA
CACTCTTGAAACAATCTTCGCGCCGATCGACTGGTTCGATACCCTTGAATTCACCGAGTCTGACGCCATCTCGATGGAGT
GCAGCAATCTTGATCTGCTGGTTGATGACTCGAATCTCTGCATCCGCGCTGCCAAAGCGTTGCAGGAGCACACCGGGGTG
AAACGCGGGGCGACTATCAAACTGCTCAAGCGGGTGCCATTTGGCGCGGGTCTCGGTGGTGGGAGCAGCGATGCGGCGGC
GACGCTCAACGCGCTGTGCAAGCTCTGGCAGATCGACGTGCCCTCGGCGGAGCTGCACAAACTCGCCGTCAAGCTTGGCG
CGGACGTGCCCTATTTCCTCGAAATGAAGGGACTTGCCTACGCTGCCGGAATCGGCGAGGAGCTCGAAGACCTCAATCTT
GCCCTGCCCTGGCACGTAGTAACGGTCTTCCCTGAGGTGCAGGTGCCGACTGCCTGGGCCTACAAGAATTTCCACCGCCA
GTTCGAGCGTCCGGTGCCCGATCTGAAAACTCTCGTTCGTCGGCTCTGCCACGAGAGAGACATCTCGGTGTTCGGTGTTT
TCGAGAATGATTTCGCCTCGGTGGTGTTCGAGCACTATCCGGTGGTTCGCGAGGTTCGCGACGCGCTCGCGGCCTCTGGC
GCCCAGTTCGTCTCGCTCTCCGGCAGCGGCTCTGCGGTCTATGCCCTTTATGAAGGGCGTGCCGACGCGGTCAAGGCCGC
CGAAGCGATGAGCGCTCGCTTCCGTATCAACATGACTCCGGCAGGTTTCCGGATGGAGTGA

Upstream 100 bases:

>100_bases
GGTGAGAGATTCGTTGCACAGTTTCAGCCAGCCGGAGGATCCCTGCGGCAGGGTCTTAGAGTGAATATGGGAAATATTTT
ACGATAAAATCAATTGCTCC

Downstream 100 bases:

>100_bases
CCGATGTTGCGGCAGCTCCGGTTTTCACCCCTGATGCTTTGCGCTCAGCATGGTTTCGAGCTGGGAGGCAAAGAGCTTGG
TGTCCTCGTCGAGGAAGTGT

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MKHFSVKACAKINLGLLITSRRADGYHTLETIFAPIDWFDTLEFTESDAISMECSNLDLLVDDSNLCIRAAKALQEHTGV
KRGATIKLLKRVPFGAGLGGGSSDAAATLNALCKLWQIDVPSAELHKLAVKLGADVPYFLEMKGLAYAAGIGEELEDLNL
ALPWHVVTVFPEVQVPTAWAYKNFHRQFERPVPDLKTLVRRLCHERDISVFGVFENDFASVVFEHYPVVREVRDALAASG
AQFVSLSGSGSAVYALYEGRADAVKAAEAMSARFRINMTPAGFRME

Sequences:

>Translated_286_residues
MKHFSVKACAKINLGLLITSRRADGYHTLETIFAPIDWFDTLEFTESDAISMECSNLDLLVDDSNLCIRAAKALQEHTGV
KRGATIKLLKRVPFGAGLGGGSSDAAATLNALCKLWQIDVPSAELHKLAVKLGADVPYFLEMKGLAYAAGIGEELEDLNL
ALPWHVVTVFPEVQVPTAWAYKNFHRQFERPVPDLKTLVRRLCHERDISVFGVFENDFASVVFEHYPVVREVRDALAASG
AQFVSLSGSGSAVYALYEGRADAVKAAEAMSARFRINMTPAGFRME
>Mature_286_residues
MKHFSVKACAKINLGLLITSRRADGYHTLETIFAPIDWFDTLEFTESDAISMECSNLDLLVDDSNLCIRAAKALQEHTGV
KRGATIKLLKRVPFGAGLGGGSSDAAATLNALCKLWQIDVPSAELHKLAVKLGADVPYFLEMKGLAYAAGIGEELEDLNL
ALPWHVVTVFPEVQVPTAWAYKNFHRQFERPVPDLKTLVRRLCHERDISVFGVFENDFASVVFEHYPVVREVRDALAASG
AQFVSLSGSGSAVYALYEGRADAVKAAEAMSARFRINMTPAGFRME

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=268, Percent_Identity=33.5820895522388, Blast_Score=107, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_CHLTE (Q8KCC7)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_662380.1
- ProteinModelPortal:   Q8KCC7
- GeneID:   1007666
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT1495
- NMPDR:   fig|194439.1.peg.1474
- TIGR:   CT1495
- HOGENOM:   HBG734593
- OMA:   VSTKEVY
- ProtClustDB:   PRK14616
- BioCyc:   CTEP194439:CT_1495-MONOMER
- BRENDA:   2.7.1.148
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 31409; Mature: 31409

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 135-135

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHFSVKACAKINLGLLITSRRADGYHTLETIFAPIDWFDTLEFTESDAISMECSNLDLL
CCCCCCCHHEEECEEEEEEECCCCCHHHHHHHHHCCCHHHHHCCCCCCCEEEEECCEEEE
VDDSNLCIRAAKALQEHTGVKRGATIKLLKRVPFGAGLGGGSSDAAATLNALCKLWQIDV
EECCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
PSAELHKLAVKLGADVPYFLEMKGLAYAAGIGEELEDLNLALPWHVVTVFPEVQVPTAWA
CHHHHHHHHHHHCCCCCCEEEECCCEEECCCCCHHHHCCEECCEEEEEEECCCCCCHHHH
YKNFHRQFERPVPDLKTLVRRLCHERDISVFGVFENDFASVVFEHYPVVREVRDALAASG
HHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHCHHHHHHHHHHHCCC
AQFVSLSGSGSAVYALYEGRADAVKAAEAMSARFRINMTPAGFRME
CEEEEECCCCCEEEEEECCCHHHHHHHHHHHCEEEEEECCCCCCCC
>Mature Secondary Structure
MKHFSVKACAKINLGLLITSRRADGYHTLETIFAPIDWFDTLEFTESDAISMECSNLDLL
CCCCCCCHHEEECEEEEEEECCCCCHHHHHHHHHCCCHHHHHCCCCCCCEEEEECCEEEE
VDDSNLCIRAAKALQEHTGVKRGATIKLLKRVPFGAGLGGGSSDAAATLNALCKLWQIDV
EECCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
PSAELHKLAVKLGADVPYFLEMKGLAYAAGIGEELEDLNLALPWHVVTVFPEVQVPTAWA
CHHHHHHHHHHHCCCCCCEEEECCCEEECCCCCHHHHCCEECCEEEEEEECCCCCCHHHH
YKNFHRQFERPVPDLKTLVRRLCHERDISVFGVFENDFASVVFEHYPVVREVRDALAASG
HHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHCHHHHHHHHHHHCCC
AQFVSLSGSGSAVYALYEGRADAVKAAEAMSARFRINMTPAGFRME
CEEEEECCCCCEEEEEECCCHHHHHHHHHHHCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901