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The map label for this gene is nadE2 [H]

Identifier: 21674314

GI number: 21674314

Start: 1398423

End: 1399283

Strand: Direct

Name: nadE2 [H]

Synonym: CT1494

Alternate gene names: 21674314

Gene position: 1398423-1399283 (Clockwise)

Preceding gene: 21674313

Following gene: 21674316

Centisome position: 64.89

GC content: 60.98

Gene sequence:

>861_bases
ATGCTCAAGTCAAAGCTCAGAATCGTTCAGGCCGACTGCACGCTGGCCAATTTCGAGGAAAATCTCGAACGCCACATCAA
AGCCATCGAAACGGCGATCCGTGACGGCGCGGACGCCATCGCCTTCCCGGAGCTGTCGCTGACCGGCTACAACGTGCAGG
ACGCTGCGCAAGACATGGCGATGCACATCGACGACCGGCGGCTCGATGCGCTTCGCGAGCTGAGCCGCGACATCTGTATC
TTCTGCGGGGGAATCGAGCTGAGCGACGACTACGGCGTCTACAACTCGGCCTTCATGTTCGAGGATGGCGCAGGCCGGAG
CGTGCACCGCAAGATCTATCTGCCGACCTACGGCATGTTCGAGGAGCTGCGATATTTCTCGGCGGGACGGCAGATAGAAA
CCGTGACCTCGCGACGCATCGGCAAGGTCGGTGTCGCTATCTGCGAGGATTTCTGGCATATGTCGGTGCCCTACCTGCTG
GCCCACCAGGGAGCGAAGCTTTTGCTCGTGCTCATGTCGAGCCCGCTGCGCCTCTCACCCGGTCAGGGCGTTCCGGCCAT
CGTGACGCAGTGGCAGACCATCGCTTCGACCAGCGCGTTTCTGCTCAGTTGTTACGTTGCCTGCGTCAACCGCGTGGGCA
ACGAGGACAGCTTCACCTACTGGGGCAACTCGGCAGTCACCACACCCGACGGATCGATCGCGGCATCCGCGCCGATGTTC
AGCGAGCACAGCTTCGACGCGACTATCGACTACTCGGTGGTCAAGCGGGTACGGCTCCAGTCATCACACTTCCTCGACGA
GGACACCAAGCTCTTTGCCTCCCAGCTCGAAACCATGCTGAGCGCAAAGCATCAGGGGTGA

Upstream 100 bases:

>100_bases
CGCTAGCGGGAAGATTCCGCGAGATCAATGCTGGCATGACCGCGCTGGCGATCCTCTCCGCAACCTTCTATATTTTCTAT
CCTTACCATTAAGAGAACCG

Downstream 100 bases:

>100_bases
AAACCGGAGCTGCCGCAACATCGGTCACTCCATCCGGAAACCTGCCGGAGTCATGTTGATACGGAAGCGAGCGCTCATCG
CTTCGGCGGCCTTGACCGCG

Product: carbon-nitrogen hydrolase family protein

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MLKSKLRIVQADCTLANFEENLERHIKAIETAIRDGADAIAFPELSLTGYNVQDAAQDMAMHIDDRRLDALRELSRDICI
FCGGIELSDDYGVYNSAFMFEDGAGRSVHRKIYLPTYGMFEELRYFSAGRQIETVTSRRIGKVGVAICEDFWHMSVPYLL
AHQGAKLLLVLMSSPLRLSPGQGVPAIVTQWQTIASTSAFLLSCYVACVNRVGNEDSFTYWGNSAVTTPDGSIAASAPMF
SEHSFDATIDYSVVKRVRLQSSHFLDEDTKLFASQLETMLSAKHQG

Sequences:

>Translated_286_residues
MLKSKLRIVQADCTLANFEENLERHIKAIETAIRDGADAIAFPELSLTGYNVQDAAQDMAMHIDDRRLDALRELSRDICI
FCGGIELSDDYGVYNSAFMFEDGAGRSVHRKIYLPTYGMFEELRYFSAGRQIETVTSRRIGKVGVAICEDFWHMSVPYLL
AHQGAKLLLVLMSSPLRLSPGQGVPAIVTQWQTIASTSAFLLSCYVACVNRVGNEDSFTYWGNSAVTTPDGSIAASAPMF
SEHSFDATIDYSVVKRVRLQSSHFLDEDTKLFASQLETMLSAKHQG
>Mature_286_residues
MLKSKLRIVQADCTLANFEENLERHIKAIETAIRDGADAIAFPELSLTGYNVQDAAQDMAMHIDDRRLDALRELSRDICI
FCGGIELSDDYGVYNSAFMFEDGAGRSVHRKIYLPTYGMFEELRYFSAGRQIETVTSRRIGKVGVAICEDFWHMSVPYLL
AHQGAKLLLVLMSSPLRLSPGQGVPAIVTQWQTIASTSAFLLSCYVACVNRVGNEDSFTYWGNSAVTTPDGSIAASAPMF
SEHSFDATIDYSVVKRVRLQSSHFLDEDTKLFASQLETMLSAKHQG

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR000132
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 31773; Mature: 31773

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKSKLRIVQADCTLANFEENLERHIKAIETAIRDGADAIAFPELSLTGYNVQDAAQDMA
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCEEECCCCHHHHHHHHH
MHIDDRRLDALRELSRDICIFCGGIELSDDYGVYNSAFMFEDGAGRSVHRKIYLPTYGMF
HHCCHHHHHHHHHHCCCEEEEECCEEECCCCCCCCCEEEEECCCCCCEEEEEECCCHHHH
EELRYFSAGRQIETVTSRRIGKVGVAICEDFWHMSVPYLLAHQGAKLLLVLMSSPLRLSP
HHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHEEEEECCCCCEECC
GQGVPAIVTQWQTIASTSAFLLSCYVACVNRVGNEDSFTYWGNSAVTTPDGSIAASAPMF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEECCCCCCEECCCCC
SEHSFDATIDYSVVKRVRLQSSHFLDEDTKLFASQLETMLSAKHQG
CCCCCCCEECHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLKSKLRIVQADCTLANFEENLERHIKAIETAIRDGADAIAFPELSLTGYNVQDAAQDMA
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCEEECCCCHHHHHHHHH
MHIDDRRLDALRELSRDICIFCGGIELSDDYGVYNSAFMFEDGAGRSVHRKIYLPTYGMF
HHCCHHHHHHHHHHCCCEEEEECCEEECCCCCCCCCEEEEECCCCCCEEEEEECCCHHHH
EELRYFSAGRQIETVTSRRIGKVGVAICEDFWHMSVPYLLAHQGAKLLLVLMSSPLRLSP
HHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHEEEEECCCCCEECC
GQGVPAIVTQWQTIASTSAFLLSCYVACVNRVGNEDSFTYWGNSAVTTPDGSIAASAPMF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEECCCCCCEECCCCC
SEHSFDATIDYSVVKRVRLQSSHFLDEDTKLFASQLETMLSAKHQG
CCCCCCCEECHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]