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The map label for this gene is dut

Identifier: 21674239

GI number: 21674239

Start: 1329226

End: 1329684

Strand: Reverse

Name: dut

Synonym: CT1418

Alternate gene names: 21674239

Gene position: 1329684-1329226 (Counterclockwise)

Preceding gene: 21674245

Following gene: 21674233

Centisome position: 61.7

GC content: 60.57

Gene sequence:

>459_bases
ATGATCAAGGTAAAAATAGTTCGACTCAATCAAAAGGCGATCCTGCCGGTCTATGCAACCGCTCACGCTGCCGGGATGGA
TGTTTCGGCATGTCTCGATGCGCCGGTGACCGTTCCGTCCTCCGCTTCGGCGCTCATTCCAACCGGCTTCGCCATCGAGC
TGCCCGAAGGCTACGAGGCGCAGCTCAGGCCGAGAAGCGGGCTGGCGCTCCGCCATTGTATCTCCCTGCCCAACTCGCCG
GCGACTATCGACGCCGACTATCGCGGCGAGGTGGGGGTCATCCTTATCAATCACGGGCGCGAGCCGTTTACGGTCAGCCA
CGGCGACCGCATTGCGCAGATGGTGGTGGCGAAGGTCGATCACGTCGTGTTCGAGGAGGTTGAATCGCTATCTGAAACCG
CGAGAGGAGAGGGGGGATTCGGGCATACAGGGGTTCAGGCGAAAGCCGAATGCCTTTGA

Upstream 100 bases:

>100_bases
GTAAACCGGTTGGATGGGTGCAGTCAAGGTCATGATTTTATATATTAAGCGACGCGGTTTTCAGCCCACGAAGGCTATCA
GGTAAAAAAAGATACACTCG

Downstream 100 bases:

>100_bases
TCTGTTCTATTCATTCCACGCAGGGCAAATCTTGACGCCTGGCTATAAAAAAGTCAGGGCGAGGTTGAACCCCGCCCTGG
TCATACGATCAAAAGTTTTT

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 152; Mature: 152

Protein sequence:

>152_residues
MIKVKIVRLNQKAILPVYATAHAAGMDVSACLDAPVTVPSSASALIPTGFAIELPEGYEAQLRPRSGLALRHCISLPNSP
ATIDADYRGEVGVILINHGREPFTVSHGDRIAQMVVAKVDHVVFEEVESLSETARGEGGFGHTGVQAKAECL

Sequences:

>Translated_152_residues
MIKVKIVRLNQKAILPVYATAHAAGMDVSACLDAPVTVPSSASALIPTGFAIELPEGYEAQLRPRSGLALRHCISLPNSP
ATIDADYRGEVGVILINHGREPFTVSHGDRIAQMVVAKVDHVVFEEVESLSETARGEGGFGHTGVQAKAECL
>Mature_152_residues
MIKVKIVRLNQKAILPVYATAHAAGMDVSACLDAPVTVPSSASALIPTGFAIELPEGYEAQLRPRSGLALRHCISLPNSP
ATIDADYRGEVGVILINHGREPFTVSHGDRIAQMVVAKVDHVVFEEVESLSETARGEGGFGHTGVQAKAECL

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI70906444, Length=143, Percent_Identity=41.958041958042, Blast_Score=118, Evalue=2e-27,
Organism=Homo sapiens, GI4503423, Length=143, Percent_Identity=41.958041958042, Blast_Score=117, Evalue=3e-27,
Organism=Homo sapiens, GI70906441, Length=143, Percent_Identity=41.958041958042, Blast_Score=117, Evalue=5e-27,
Organism=Escherichia coli, GI1790071, Length=148, Percent_Identity=42.5675675675676, Blast_Score=119, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI71988561, Length=143, Percent_Identity=44.0559440559441, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6319729, Length=142, Percent_Identity=38.7323943661972, Blast_Score=103, Evalue=2e-23,
Organism=Drosophila melanogaster, GI19921126, Length=143, Percent_Identity=39.1608391608392, Blast_Score=114, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24583610, Length=143, Percent_Identity=39.1608391608392, Blast_Score=114, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_CHLTE (O68992)

Other databases:

- EMBL:   AF060080
- EMBL:   AY005138
- EMBL:   AE006470
- RefSeq:   NP_662304.1
- ProteinModelPortal:   O68992
- SMR:   O68992
- GeneID:   1006834
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT1418
- NMPDR:   fig|194439.1.peg.1398
- TIGR:   CT1418
- HOGENOM:   HBG436079
- OMA:   LDLRACI
- ProtClustDB:   CLSK637750
- BioCyc:   CTEP194439:CT_1418-MONOMER
- BRENDA:   3.6.1.23
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 16099; Mature: 16099

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: BINDING 78-78

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKVKIVRLNQKAILPVYATAHAAGMDVSACLDAPVTVPSSASALIPTGFAIELPEGYEA
CEEEEEEEECCCEEEEEEEECCCCCCCHHHHCCCCCCCCCCCCEEECCCEEEECCCCCCE
QLRPRSGLALRHCISLPNSPATIDADYRGEVGVILINHGREPFTVSHGDRIAQMVVAKVD
EECCCCCHHHHHHHCCCCCCCEEECCCCCCEEEEEEECCCCCEEECCCHHHHHHHHHHHH
HVVFEEVESLSETARGEGGFGHTGVQAKAECL
HHHHHHHHHHHHHHCCCCCCCCCCCCEEECCC
>Mature Secondary Structure
MIKVKIVRLNQKAILPVYATAHAAGMDVSACLDAPVTVPSSASALIPTGFAIELPEGYEA
CEEEEEEEECCCEEEEEEEECCCCCCCHHHHCCCCCCCCCCCCEEECCCEEEECCCCCCE
QLRPRSGLALRHCISLPNSPATIDADYRGEVGVILINHGREPFTVSHGDRIAQMVVAKVD
EECCCCCHHHHHHHCCCCCCCEEECCCCCCEEEEEEECCCCCEEECCCHHHHHHHHHHHH
HVVFEEVESLSETARGEGGFGHTGVQAKAECL
HHHHHHHHHHHHHHCCCCCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10976061; 12093901