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The map label for this gene is thiE

Identifier: 21674001

GI number: 21674001

Start: 1104770

End: 1105384

Strand: Direct

Name: thiE

Synonym: CT1175

Alternate gene names: 21674001

Gene position: 1104770-1105384 (Clockwise)

Preceding gene: 21674000

Following gene: 21674002

Centisome position: 51.27

GC content: 59.84

Gene sequence:

>615_bases
ATGACGTTACCGCGAAGAGTTCTCTGCGTCATCACCGATGAACACTCCAATCCAGTCGAACTTGCCCGCATGGCCCTCCA
GGGCGGGGCAGGGATGGTACAGTTACGAAGAAAAACAGCCTCGGGCCAAGAGCTTTACGAGTGGGCCATCAGAATTCAAG
CGCTTTGCAGTGAACAGCAGGCGCTCTTCATCGTCAATGACCGGGTCGATATTGCCATGGCCGTACATGCCGATGGAGTG
CACCTCGGCCAACAGGATCTGCCCGCGTCGGCTGCCCGCGCACTGCTTGCCCCCGATGCGATCATTGGCGTATCGGTCTC
CAACGCCACCGAGGCTATCAAGGCAGCCGAAGAGGGCGCAAGCTATATCGGCGTGGGGCACATATTCCCGACTTTTTCCA
AAGACAAACCTTCAGAGCCGCTCGGCACGGCATCTATACGACCGATCGGCAGGGCTGCGCAGCTGCCGGTCATCGCCATC
GGCGGCATCGGGCACGACAACGCTGCCGAGGTCATCCGTGCCGGTGCATCAGGCATCGCCGTCATCTCCGCGGTTTCCGA
CAGCGACGATCCCGAGACCGCCACTCGCGAGCTGGTAAGAAGAATCAGGCAATAA

Upstream 100 bases:

>100_bases
GCATCGAATGCGGAGCCTGGGGCGTAGCCGCCCTCACACCGTTTCTCGATGCCGGATCGCTGCCTGAAACCGTCAACCGC
TTCTACTCATTCATGCAATC

Downstream 100 bases:

>100_bases
ATATGATGCAGAACTACATCACCGTGCTGACCATCGCCGGATCGGACGGCAGCGGCGGAGCTGGCATCCAGACCGACCTG
AAAACCATCGCCGCCAACAA

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MTLPRRVLCVITDEHSNPVELARMALQGGAGMVQLRRKTASGQELYEWAIRIQALCSEQQALFIVNDRVDIAMAVHADGV
HLGQQDLPASAARALLAPDAIIGVSVSNATEAIKAAEEGASYIGVGHIFPTFSKDKPSEPLGTASIRPIGRAAQLPVIAI
GGIGHDNAAEVIRAGASGIAVISAVSDSDDPETATRELVRRIRQ

Sequences:

>Translated_204_residues
MTLPRRVLCVITDEHSNPVELARMALQGGAGMVQLRRKTASGQELYEWAIRIQALCSEQQALFIVNDRVDIAMAVHADGV
HLGQQDLPASAARALLAPDAIIGVSVSNATEAIKAAEEGASYIGVGHIFPTFSKDKPSEPLGTASIRPIGRAAQLPVIAI
GGIGHDNAAEVIRAGASGIAVISAVSDSDDPETATRELVRRIRQ
>Mature_203_residues
TLPRRVLCVITDEHSNPVELARMALQGGAGMVQLRRKTASGQELYEWAIRIQALCSEQQALFIVNDRVDIAMAVHADGVH
LGQQDLPASAARALLAPDAIIGVSVSNATEAIKAAEEGASYIGVGHIFPTFSKDKPSEPLGTASIRPIGRAAQLPVIAIG
GIGHDNAAEVIRAGASGIAVISAVSDSDDPETATRELVRRIRQ

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=184, Percent_Identity=35.8695652173913, Blast_Score=96, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6325042, Length=191, Percent_Identity=33.5078534031414, Blast_Score=106, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_CHLTE (Q8KD79)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_662066.1
- ProteinModelPortal:   Q8KD79
- SMR:   Q8KD79
- GeneID:   1006539
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT1175
- NMPDR:   fig|194439.1.peg.1160
- TIGR:   CT1175
- HOGENOM:   HBG754477
- OMA:   IMGSNNC
- ProtClustDB:   CLSK637633
- BioCyc:   CTEP194439:CT_1175-MONOMER
- BRENDA:   2.5.1.3
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 21475; Mature: 21344

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLPRRVLCVITDEHSNPVELARMALQGGAGMVQLRRKTASGQELYEWAIRIQALCSEQQ
CCCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEHHCCCHHHHHHHHHHHHHHHCCCC
ALFIVNDRVDIAMAVHADGVHLGQQDLPASAARALLAPDAIIGVSVSNATEAIKAAEEGA
EEEEEECCEEEEEEEECCCCCCCCCCCCHHHHHHHHCCCEEEEEEECCHHHHHHHHHCCC
SYIGVGHIFPTFSKDKPSEPLGTASIRPIGRAAQLPVIAIGGIGHDNAAEVIRAGASGIA
CEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHCCCCCEE
VISAVSDSDDPETATRELVRRIRQ
EEEECCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TLPRRVLCVITDEHSNPVELARMALQGGAGMVQLRRKTASGQELYEWAIRIQALCSEQQ
CCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEHHCCCHHHHHHHHHHHHHHHCCCC
ALFIVNDRVDIAMAVHADGVHLGQQDLPASAARALLAPDAIIGVSVSNATEAIKAAEEGA
EEEEEECCEEEEEEEECCCCCCCCCCCCHHHHHHHHCCCEEEEEEECCHHHHHHHHHCCC
SYIGVGHIFPTFSKDKPSEPLGTASIRPIGRAAQLPVIAIGGIGHDNAAEVIRAGASGIA
CEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHCCCCCEE
VISAVSDSDDPETATRELVRRIRQ
EEEECCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901