Click here to switch to the map view.

The map label for this gene is thiE [C]

Identifier: 21674000

GI number: 21674000

Start: 1104117

End: 1104773

Strand: Direct

Name: thiE [C]

Synonym: CT1174

Alternate gene names: 21674000

Gene position: 1104117-1104773 (Clockwise)

Preceding gene: 21673994

Following gene: 21674001

Centisome position: 51.24

GC content: 53.88

Gene sequence:

>657_bases
GTGCCTGAAATTGCATTTATGACTGAAAAACACCCCTCACTCCCCCGGCTGATGATTGTCAGCAGTGGCGGCGAACATTT
TTCTCAGAAAGGCCTTGTACTGGCACAGGCACAAACTCTCGCCCGCTCTGCGCCGGTCATCTTCCAGGTTCGAGAGAAAA
TGCTCGATTCTGCTTCGATGTGGCGCCTCTGTAGCCAAATCGCCCCGCTTGTCGATAATTCGGGATCGATTCTGACCATC
AACGAACGCTTCGACATCGCACTTGCATCAAAGGCGGGCGGAGTTCATCTTCCCGAATCTTCATGCCCTGCCGATGTCGT
TCGAAAAACTGCACGAAAATTGCTTGTCGGGCAAAGCGTACATAGCGAAACGACAGCATTGAAAGCCGCTTCGACAGGCC
TTGATTACCTCCTTTTCGGACCAGTATTCCATACGCCATCAAAAGCATCTTTCGGCCCGCCTCAGGGGTTGGATCGCCTA
CGGGAAATCTGCGAAAAAGTACGCATCCCGGTCTTCGCCGTTGGAGGCATCACGCCTGAAAAAGTCCCGGCCTGCATCGA
ATGCGGAGCCTGGGGCGTAGCCGCCCTCACACCGTTTCTCGATGCCGGATCGCTGCCTGAAACCGTCAACCGCTTCTACT
CATTCATGCAATCATGA

Upstream 100 bases:

>100_bases
ATCATGACCTTTTTGTGTTTTCTGTTTGGGAAAAGAGCGGAAAATCCGAACAGTTAGGGATAGATTTTCCAGATTGCGAT
TCCTTTGGAAAATAGTTATC

Downstream 100 bases:

>100_bases
CGTTACCGCGAAGAGTTCTCTGCGTCATCACCGATGAACACTCCAATCCAGTCGAACTTGCCCGCATGGCCCTCCAGGGC
GGGGCAGGGATGGTACAGTT

Product: thiamine-phosphate pyrophosphorylase, putative

Products: diphosphate; thiamine monophosphate

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MPEIAFMTEKHPSLPRLMIVSSGGEHFSQKGLVLAQAQTLARSAPVIFQVREKMLDSASMWRLCSQIAPLVDNSGSILTI
NERFDIALASKAGGVHLPESSCPADVVRKTARKLLVGQSVHSETTALKAASTGLDYLLFGPVFHTPSKASFGPPQGLDRL
REICEKVRIPVFAVGGITPEKVPACIECGAWGVAALTPFLDAGSLPETVNRFYSFMQS

Sequences:

>Translated_218_residues
MPEIAFMTEKHPSLPRLMIVSSGGEHFSQKGLVLAQAQTLARSAPVIFQVREKMLDSASMWRLCSQIAPLVDNSGSILTI
NERFDIALASKAGGVHLPESSCPADVVRKTARKLLVGQSVHSETTALKAASTGLDYLLFGPVFHTPSKASFGPPQGLDRL
REICEKVRIPVFAVGGITPEKVPACIECGAWGVAALTPFLDAGSLPETVNRFYSFMQS
>Mature_217_residues
PEIAFMTEKHPSLPRLMIVSSGGEHFSQKGLVLAQAQTLARSAPVIFQVREKMLDSASMWRLCSQIAPLVDNSGSILTIN
ERFDIALASKAGGVHLPESSCPADVVRKTARKLLVGQSVHSETTALKAASTGLDYLLFGPVFHTPSKASFGPPQGLDRLR
EICEKVRIPVFAVGGITPEKVPACIECGAWGVAALTPFLDAGSLPETVNRFYSFMQS

Specific function: Condenses 4-Methyl-5-(Beta-Hydroxyethyl)-Thiazole Monophosphate (Thz-P) And 4-Amino-5-Hydroxymethyl Pyrimidine Pyrophosphate (Hmp-Pp) To Form Thiamine Monophosphate (TMP). [C]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: 2.5.1.3

Molecular weight: Translated: 23437; Mature: 23306

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEIAFMTEKHPSLPRLMIVSSGGEHFSQKGLVLAQAQTLARSAPVIFQVREKMLDSASM
CCCCCCCCCCCCCCCEEEEEECCCCHHHHCCEEHHHHHHHHHCCCHHHHHHHHHHCHHHH
WRLCSQIAPLVDNSGSILTINERFDIALASKAGGVHLPESSCPADVVRKTARKLLVGQSV
HHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCH
HSETTALKAASTGLDYLLFGPVFHTPSKASFGPPQGLDRLREICEKVRIPVFAVGGITPE
HHHHHHHHHHHCCCCEEEECCEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCHH
KVPACIECGAWGVAALTPFLDAGSLPETVNRFYSFMQS
HCCHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
PEIAFMTEKHPSLPRLMIVSSGGEHFSQKGLVLAQAQTLARSAPVIFQVREKMLDSASM
CCCCCCCCCCCCCCEEEEEECCCCHHHHCCEEHHHHHHHHHCCCHHHHHHHHHHCHHHH
WRLCSQIAPLVDNSGSILTINERFDIALASKAGGVHLPESSCPADVVRKTARKLLVGQSV
HHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCH
HSETTALKAASTGLDYLLFGPVFHTPSKASFGPPQGLDRLREICEKVRIPVFAVGGITPE
HHHHHHHHHHHCCCCEEEECCEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCHH
KVPACIECGAWGVAALTPFLDAGSLPETVNRFYSFMQS
HCCHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Ca2+; Co2+; Mg2+; Mn2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0004 {thiazole} 0.00085 {hydroxymethylpyrimidine} [C]

Substrates: 2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate; 4-Methyl-5-(2-phosphono-oxyethyl)-thiazole

Specific reaction: 2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine monophosphate

General reaction: Hydroxymethylpyrimidine group transfer [C]

Inhibitor: Acetyl phosphate substrates; ADP; ATP; CTP; Diphosphate; GTP; Phosphocreatine; Phosphoenolpyruvate; UTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]