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The map label for this gene is thiD [H]

Identifier: 21674002

GI number: 21674002

Start: 1105387

End: 1106220

Strand: Direct

Name: thiD [H]

Synonym: CT1176

Alternate gene names: 21674002

Gene position: 1105387-1106220 (Clockwise)

Preceding gene: 21674001

Following gene: 21674005

Centisome position: 51.3

GC content: 55.16

Gene sequence:

>834_bases
ATGATGCAGAACTACATCACCGTGCTGACCATCGCCGGATCGGACGGCAGCGGCGGAGCTGGCATCCAGACCGACCTGAA
AACCATCGCCGCCAACAACTGTTACGGCCTGAGCGTCATCACTGCTGTGACGGCACAGAACACCACTGAAGTACGGTCTA
TACACAACATTCCGCCCGCCTTCATCGGCGAGCAGTTCAAGACGATTGTCGACGATATTCGAATCGATGCGGTCAAGATC
GGCATGCTGGGCTCGCTGGAAGCGGCAGAAACAGTCGTTGAACTCATGAAAAGCCTCAATGAGGTACCGGTCGTTCTCGA
CACCGTTTTGCGCTCATCGAGCGGAAAATCGCTGCTCGACGCAGAAGCCCTGCTGGTGATGAAGCAGCTCTTTCATCTCA
CCAGTCTGATTACCCCAAATCTGCCAGAGGCGGCAATACTGACGGGCCGCAGCATGGCTCCAACAACGCAGGCTGAAATC
GAAGTGATGGCAAAAGATTTGCAACGGGAAGGCGCAAAATCCGTTCTTGTCAAGGGAGGACATGGCGAGGGAGACCAATG
CAACGACTGCCTGCTGCACGAGGGACAGCTCTTTTGGTACTCAAACCCGAAAATCGATACACTCAACACACATGGCACCG
GCTGCACACTCTCGTCAGCCATAGCTTGCGGACTGGCAAAAGGCTTGCCGATGAATGAAGCTGTGGCAGAAGCTATCAGC
TATACGAGAAAAGCCCTGCTGGCAGGCGCATCATGGCGGCTGGGACACGGAAATGGTCCGCTGGAGCATTTTCCCGACCG
GACAGTCGAAAGACGGCCGGGAAAACTGCAATAA

Upstream 100 bases:

>100_bases
GTGCCGGTGCATCAGGCATCGCCGTCATCTCCGCGGTTTCCGACAGCGACGATCCCGAGACCGCCACTCGCGAGCTGGTA
AGAAGAATCAGGCAATAAAT

Downstream 100 bases:

>100_bases
AACAAGCTTAGCGCACCAGGCGAGGACGACGGCCGATAGAGTAGTAGCTGATGCCCACCTGTTCCACCATATCGGGATTG
TAGATATTCCTGCCGTCGAA

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Thiamine-phosphate pyrophosphorylase; TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase; Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MMQNYITVLTIAGSDGSGGAGIQTDLKTIAANNCYGLSVITAVTAQNTTEVRSIHNIPPAFIGEQFKTIVDDIRIDAVKI
GMLGSLEAAETVVELMKSLNEVPVVLDTVLRSSSGKSLLDAEALLVMKQLFHLTSLITPNLPEAAILTGRSMAPTTQAEI
EVMAKDLQREGAKSVLVKGGHGEGDQCNDCLLHEGQLFWYSNPKIDTLNTHGTGCTLSSAIACGLAKGLPMNEAVAEAIS
YTRKALLAGASWRLGHGNGPLEHFPDRTVERRPGKLQ

Sequences:

>Translated_277_residues
MMQNYITVLTIAGSDGSGGAGIQTDLKTIAANNCYGLSVITAVTAQNTTEVRSIHNIPPAFIGEQFKTIVDDIRIDAVKI
GMLGSLEAAETVVELMKSLNEVPVVLDTVLRSSSGKSLLDAEALLVMKQLFHLTSLITPNLPEAAILTGRSMAPTTQAEI
EVMAKDLQREGAKSVLVKGGHGEGDQCNDCLLHEGQLFWYSNPKIDTLNTHGTGCTLSSAIACGLAKGLPMNEAVAEAIS
YTRKALLAGASWRLGHGNGPLEHFPDRTVERRPGKLQ
>Mature_277_residues
MMQNYITVLTIAGSDGSGGAGIQTDLKTIAANNCYGLSVITAVTAQNTTEVRSIHNIPPAFIGEQFKTIVDDIRIDAVKI
GMLGSLEAAETVVELMKSLNEVPVVLDTVLRSSSGKSLLDAEALLVMKQLFHLTSLITPNLPEAAILTGRSMAPTTQAEI
EVMAKDLQREGAKSVLVKGGHGEGDQCNDCLLHEGQLFWYSNPKIDTLNTHGTGCTLSSAIACGLAKGLPMNEAVAEAIS
YTRKALLAGASWRLGHGNGPLEHFPDRTVERRPGKLQ

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiaminase-2 family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=261, Percent_Identity=44.8275862068966, Blast_Score=196, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6325378, Length=269, Percent_Identity=32.7137546468402, Blast_Score=142, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6324997, Length=270, Percent_Identity=36.2962962962963, Blast_Score=128, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6324517, Length=270, Percent_Identity=35.1851851851852, Blast_Score=122, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR016084
- InterPro:   IPR013749
- InterPro:   IPR004399
- InterPro:   IPR004305
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF08543 Phos_pyr_kin; PF03070 TENA_THI-4; PF02581 TMP-TENI [H]

EC number: =2.5.1.3; =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 29464; Mature: 29464

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMQNYITVLTIAGSDGSGGAGIQTDLKTIAANNCYGLSVITAVTAQNTTEVRSIHNIPPA
CCCCEEEEEEEECCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHCCCHH
FIGEQFKTIVDDIRIDAVKIGMLGSLEAAETVVELMKSLNEVPVVLDTVLRSSSGKSLLD
HHHHHHHHHHHHHHHCEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHH
AEALLVMKQLFHLTSLITPNLPEAAILTGRSMAPTTQAEIEVMAKDLQREGAKSVLVKGG
HHHHHHHHHHHHHHHHHCCCCCCHHEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECC
HGEGDQCNDCLLHEGQLFWYSNPKIDTLNTHGTGCTLSSAIACGLAKGLPMNEAVAEAIS
CCCCCCCCHHHEECCEEEEECCCCEEECCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
YTRKALLAGASWRLGHGNGPLEHFPDRTVERRPGKLQ
HHHHHHHCCCCEEECCCCCCHHHCCCCHHHCCCCCCC
>Mature Secondary Structure
MMQNYITVLTIAGSDGSGGAGIQTDLKTIAANNCYGLSVITAVTAQNTTEVRSIHNIPPA
CCCCEEEEEEEECCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHCCCHH
FIGEQFKTIVDDIRIDAVKIGMLGSLEAAETVVELMKSLNEVPVVLDTVLRSSSGKSLLD
HHHHHHHHHHHHHHHCEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHH
AEALLVMKQLFHLTSLITPNLPEAAILTGRSMAPTTQAEIEVMAKDLQREGAKSVLVKGG
HHHHHHHHHHHHHHHHHCCCCCCHHEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECC
HGEGDQCNDCLLHEGQLFWYSNPKIDTLNTHGTGCTLSSAIACGLAKGLPMNEAVAEAIS
CCCCCCCCHHHEECCEEEEECCCCEEECCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
YTRKALLAGASWRLGHGNGPLEHFPDRTVERRPGKLQ
HHHHHHHCCCCEEECCCCCCHHHCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]