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The map label for this gene is apbE [H]

Identifier: 21673900

GI number: 21673900

Start: 1014709

End: 1015590

Strand: Direct

Name: apbE [H]

Synonym: CT1074

Alternate gene names: 21673900

Gene position: 1014709-1015590 (Clockwise)

Preceding gene: 21673899

Following gene: 21673901

Centisome position: 47.09

GC content: 59.3

Gene sequence:

>882_bases
ATGCACAGATTCGGATTCAGGGCGATGGGCAGCGGATGCGAAATCGTGCTCGCCGGAACAACGAAAAAGAAGGCAAAAGC
GCTTGCTATGCTTGGCATGGAGGAGATTGCCTGGATTGAGCGGAAGTATTCTCGATACCAGCCTGAAAGCGTGGTGTCGC
GGATCAACGATGCCGCCGGAAAAGCGTGGGTGGCGTGCGACGACGAAACTTCGGCCCTGCTCGATTACACTGACGCGGTG
TATCGCAGTAGTGGCGGGCTGTTCGATGTGACCTCGGGCGTGCTGCGCCGGGCGTGGAATTTCGACAAAACCGAAGTGCC
TTCGCAGGAGTCGCTCTTCCCGCTGCTGAAGCTTGTCGGATGGCAACGGGTTGAGCGGCGTGAGAACGATGTCCGGCTGC
CGCAGGAGGGGATGCAGCTTGATTTCGGCGGCATCGGCAAGGAGTACGCCGCCGATGCCGCCGCGGAGATTCTCTGCGAA
GAGGGCGTTCGGCACGGCTATGTGAATCTTGGCGGAGACATCCGCGTGATCGGCCCCAAGCCCGGCGGCGAGCCGTGGAC
GATCGGCATCCGCGATCCGCGCGATCCCGCCGGAACGATCGCCTCGATTTCTGTCAGCTCCGGAGCTATTGCCACCAGCG
GAGACTACGAGCGCTTTTTCGAGGTGGAGGGGAGGCGTTACTGCCACATCATCAACCCAAAGACGGGCTTTCCGGTGAGC
CAGTGGCGTTCGGTGACAGTGCTTGCTCCGCTTGCCACAACGGCGGGCAGTTGCACTACGGTTGCCATGCTTCTGGAAGC
CGACGGGCTAAACTATCTGAAGAGTTCAGGATTCAATTATCTGGCCATCAATCAGTCGGGCGAAATGTATTATCAAGATT
GA

Upstream 100 bases:

>100_bases
CCGGCATTCAGCTTCAGAAGCCTTCAGGTCGGTCTTTCGCGCAAGTTCTGAGTCCGTGAAAAGTGGAAAAGATGCGCTAT
TGATGTAATTTGCCGTTCAT

Downstream 100 bases:

>100_bases
ACCAACGCTGAAAAAAATGATAAAAAAATCAACCCCAAGAAGCTTTTTCGCAGTTGTCGGAATCCTGCTGATGCTTTTCA
TGAGAAGTGCTTCGGCTATG

Product: ApbE family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MHRFGFRAMGSGCEIVLAGTTKKKAKALAMLGMEEIAWIERKYSRYQPESVVSRINDAAGKAWVACDDETSALLDYTDAV
YRSSGGLFDVTSGVLRRAWNFDKTEVPSQESLFPLLKLVGWQRVERRENDVRLPQEGMQLDFGGIGKEYAADAAAEILCE
EGVRHGYVNLGGDIRVIGPKPGGEPWTIGIRDPRDPAGTIASISVSSGAIATSGDYERFFEVEGRRYCHIINPKTGFPVS
QWRSVTVLAPLATTAGSCTTVAMLLEADGLNYLKSSGFNYLAINQSGEMYYQD

Sequences:

>Translated_293_residues
MHRFGFRAMGSGCEIVLAGTTKKKAKALAMLGMEEIAWIERKYSRYQPESVVSRINDAAGKAWVACDDETSALLDYTDAV
YRSSGGLFDVTSGVLRRAWNFDKTEVPSQESLFPLLKLVGWQRVERRENDVRLPQEGMQLDFGGIGKEYAADAAAEILCE
EGVRHGYVNLGGDIRVIGPKPGGEPWTIGIRDPRDPAGTIASISVSSGAIATSGDYERFFEVEGRRYCHIINPKTGFPVS
QWRSVTVLAPLATTAGSCTTVAMLLEADGLNYLKSSGFNYLAINQSGEMYYQD
>Mature_293_residues
MHRFGFRAMGSGCEIVLAGTTKKKAKALAMLGMEEIAWIERKYSRYQPESVVSRINDAAGKAWVACDDETSALLDYTDAV
YRSSGGLFDVTSGVLRRAWNFDKTEVPSQESLFPLLKLVGWQRVERRENDVRLPQEGMQLDFGGIGKEYAADAAAEILCE
EGVRHGYVNLGGDIRVIGPKPGGEPWTIGIRDPRDPAGTIASISVSSGAIATSGDYERFFEVEGRRYCHIINPKTGFPVS
QWRSVTVLAPLATTAGSCTTVAMLLEADGLNYLKSSGFNYLAINQSGEMYYQD

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=269, Percent_Identity=25.6505576208178, Blast_Score=106, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 32116; Mature: 32116

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRFGFRAMGSGCEIVLAGTTKKKAKALAMLGMEEIAWIERKYSRYQPESVVSRINDAAG
CCCCCCEECCCCCEEEEECCCHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHCC
KAWVACDDETSALLDYTDAVYRSSGGLFDVTSGVLRRAWNFDKTEVPSQESLFPLLKLVG
CEEEEECCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
WQRVERRENDVRLPQEGMQLDFGGIGKEYAADAAAEILCEEGVRHGYVNLGGDIRVIGPK
HHHHHHCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCEEEECCC
PGGEPWTIGIRDPRDPAGTIASISVSSGAIATSGDYERFFEVEGRRYCHIINPKTGFPVS
CCCCEEEEECCCCCCCCCCEEEEEECCCCEEECCCHHHHEEECCCEEEEEECCCCCCCHH
QWRSVTVLAPLATTAGSCTTVAMLLEADGLNYLKSSGFNYLAINQSGEMYYQD
HCCCEEEEEECCCCCCCHHHEEEEHHCCCCHHHHHCCCCEEEECCCCCEEECC
>Mature Secondary Structure
MHRFGFRAMGSGCEIVLAGTTKKKAKALAMLGMEEIAWIERKYSRYQPESVVSRINDAAG
CCCCCCEECCCCCEEEEECCCHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHCC
KAWVACDDETSALLDYTDAVYRSSGGLFDVTSGVLRRAWNFDKTEVPSQESLFPLLKLVG
CEEEEECCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
WQRVERRENDVRLPQEGMQLDFGGIGKEYAADAAAEILCEEGVRHGYVNLGGDIRVIGPK
HHHHHHCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCEEEECCC
PGGEPWTIGIRDPRDPAGTIASISVSSGAIATSGDYERFFEVEGRRYCHIINPKTGFPVS
CCCCEEEEECCCCCCCCCCEEEEEECCCCEEECCCHHHHEEECCCEEEEEECCCCCCCHH
QWRSVTVLAPLATTAGSCTTVAMLLEADGLNYLKSSGFNYLAINQSGEMYYQD
HCCCEEEEEECCCCCCCHHHEEEEHHCCCCHHHHHCCCCEEEECCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]