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The map label for this gene is purT

Identifier: 21673886

GI number: 21673886

Start: 996939

End: 998117

Strand: Direct

Name: purT

Synonym: CT1060

Alternate gene names: 21673886

Gene position: 996939-998117 (Clockwise)

Preceding gene: 21673885

Following gene: 21673889

Centisome position: 46.26

GC content: 63.36

Gene sequence:

>1179_bases
ATGCCGAAAACAATCATGCTGCTGGGCAGCGGGGAGCTGGGCAAAGAGTTCGTGATTGCCGTCAAGCGGCTGGGGCAGCG
GGTGGTCGCCGTGGACAGTTACGACGATGCGCCCGCGCAGCAGGTGGCCGACAGGCGCGAGGTGATCGACATGCTCGACG
GCCGGGCGCTCGACGCCATCGTCGCCAAGCACCGGCCCGACATCATCGTTCCGGAGATCGAGGCGATTCGCACCGAGCGA
TTCTACGATTATGAAAAGCAGGGGATTCAGGTGGTGCCCTCCGCGAGGGCGGCCAATTTTACGATGAACCGCAAGGCGAT
CCGAGACCTTGCTGCCCGCGATCTCGGCTTGCGTACGGCGAACTACCGCTATGCCTCCTCGCTTGAAGAGTTGCGGTCGG
CAGTGGGTGAAGTTGGCCTGCCCTGCGTGGTCAAGCCGCTGATGAGTTCGTCGGGCAAGGGGCAATCGACGGTGCGGAGC
GAGGCGGAGATCGAGGCGGCGTGGAGCTACTCGCAGAGCGGCAAGCGCGGCGACATCGCCGAGGTGATCGTCGAGGCTTT
CGTGCCGTTCCATACCGAAATTACGCTCTTGACCGTGACGCAAAAGAATGGCCCAACGCTCTTCTGCCCGCCCATCGGCC
ACCGGCAGGAGCGCGGCGACTATCAGGAGAGCTGGCAGCCGTGCCGCATCAGCCCGGAGCAACTGGAAGAGGCGCAGAAT
ATCGCGGGCAAAGTGACCGAGGCCTTGACCGGGGCGGGCATCTGGGGCGTGGAGTTTTTCCTTGCCGACGACGGCGTCTA
CTTTTCGGAGCTTTCGCCGCGGCCGCACGATACCGGCATGGTGACGCTCGCCGGAACGCAGAACCTCTCGGAGTTCGAGC
TGCACGCCCGCGCGGTGCTCGGCTTGCCGGTTCCAGATATCACGTTGCTCCGCGCCGGAGCGAGCGCCGTGATTCTCGCT
GACAGGGTGGGCAACAATCCGTCGTTTGATGGTCTGGATGCGGCGCTGGCGGAGCCGGGCAGCGACATCCGGATTTTCGG
CAAGCCGGTGATGCGTCCATACCGGCGTATGGGCGTAGCGCTTATGTCGGGTGAAAAGGGAAGCGATGTCGATGAGCTGA
AGCGGCAGGCGATTGCCAATGCCGAGAAAGTGATGATCAAGTGTGACGAGACGGTGTGA

Upstream 100 bases:

>100_bases
TGCACTGGACATCAAAGATTACGGCAAAGGAAAAGGCCCGTGAGTGGTTTGCCCAGATCAAGGCGAAAGGATTTTACAAG
TATCAAAAATAAAAAGAGTT

Downstream 100 bases:

>100_bases
AAAATTTCATCACACTTGACCGAGTCGTTTTAACGCATGTCAGTCCGCGATGGCGGAATCGGCGGATGGCTGCGTGGATT
GCTTCAGGCTGGAGGTGCGT

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MPKTIMLLGSGELGKEFVIAVKRLGQRVVAVDSYDDAPAQQVADRREVIDMLDGRALDAIVAKHRPDIIVPEIEAIRTER
FYDYEKQGIQVVPSARAANFTMNRKAIRDLAARDLGLRTANYRYASSLEELRSAVGEVGLPCVVKPLMSSSGKGQSTVRS
EAEIEAAWSYSQSGKRGDIAEVIVEAFVPFHTEITLLTVTQKNGPTLFCPPIGHRQERGDYQESWQPCRISPEQLEEAQN
IAGKVTEALTGAGIWGVEFFLADDGVYFSELSPRPHDTGMVTLAGTQNLSEFELHARAVLGLPVPDITLLRAGASAVILA
DRVGNNPSFDGLDAALAEPGSDIRIFGKPVMRPYRRMGVALMSGEKGSDVDELKRQAIANAEKVMIKCDETV

Sequences:

>Translated_392_residues
MPKTIMLLGSGELGKEFVIAVKRLGQRVVAVDSYDDAPAQQVADRREVIDMLDGRALDAIVAKHRPDIIVPEIEAIRTER
FYDYEKQGIQVVPSARAANFTMNRKAIRDLAARDLGLRTANYRYASSLEELRSAVGEVGLPCVVKPLMSSSGKGQSTVRS
EAEIEAAWSYSQSGKRGDIAEVIVEAFVPFHTEITLLTVTQKNGPTLFCPPIGHRQERGDYQESWQPCRISPEQLEEAQN
IAGKVTEALTGAGIWGVEFFLADDGVYFSELSPRPHDTGMVTLAGTQNLSEFELHARAVLGLPVPDITLLRAGASAVILA
DRVGNNPSFDGLDAALAEPGSDIRIFGKPVMRPYRRMGVALMSGEKGSDVDELKRQAIANAEKVMIKCDETV
>Mature_391_residues
PKTIMLLGSGELGKEFVIAVKRLGQRVVAVDSYDDAPAQQVADRREVIDMLDGRALDAIVAKHRPDIIVPEIEAIRTERF
YDYEKQGIQVVPSARAANFTMNRKAIRDLAARDLGLRTANYRYASSLEELRSAVGEVGLPCVVKPLMSSSGKGQSTVRSE
AEIEAAWSYSQSGKRGDIAEVIVEAFVPFHTEITLLTVTQKNGPTLFCPPIGHRQERGDYQESWQPCRISPEQLEEAQNI
AGKVTEALTGAGIWGVEFFLADDGVYFSELSPRPHDTGMVTLAGTQNLSEFELHARAVLGLPVPDITLLRAGASAVILAD
RVGNNPSFDGLDAALAEPGSDIRIFGKPVMRPYRRMGVALMSGEKGSDVDELKRQAIANAEKVMIKCDETV

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1788155, Length=375, Percent_Identity=54.1333333333333, Blast_Score=375, Evalue=1e-105,
Organism=Escherichia coli, GI1786733, Length=361, Percent_Identity=25.207756232687, Blast_Score=68, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6324702, Length=366, Percent_Identity=23.7704918032787, Blast_Score=109, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURT_CHLTE (Q8KDJ0)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_661951.1
- HSSP:   P33221
- ProteinModelPortal:   Q8KDJ0
- SMR:   Q8KDJ0
- GeneID:   1006954
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT1060
- NMPDR:   fig|194439.1.peg.1045
- TIGR:   CT1060
- HOGENOM:   HBG516369
- OMA:   TMNRKAI
- ProtClustDB:   PRK09288
- BioCyc:   CTEP194439:CT_1060-MONOMER
- HAMAP:   MF_01643
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF2
- TIGRFAMs:   TIGR01142

Pfam domain/function: PF02222 ATP-grasp; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: 2.1.2.- [C]

Molecular weight: Translated: 42783; Mature: 42652

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 72-72 BINDING 104-104 BINDING 145-145 BINDING 193-193 BINDING 258-258 BINDING 270-270 BINDING 277-277 BINDING 348-348

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKTIMLLGSGELGKEFVIAVKRLGQRVVAVDSYDDAPAQQVADRREVIDMLDGRALDAI
CCCEEEEEECCCCCHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHH
VAKHRPDIIVPEIEAIRTERFYDYEKQGIQVVPSARAANFTMNRKAIRDLAARDLGLRTA
HHHCCCCEECCCHHHHHHHHHHCHHHCCEEEECCCCCCCEEECHHHHHHHHHHHHCCCCC
NYRYASSLEELRSAVGEVGLPCVVKPLMSSSGKGQSTVRSEAEIEAAWSYSQSGKRGDIA
CCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHH
EVIVEAFVPFHTEITLLTVTQKNGPTLFCPPIGHRQERGDYQESWQPCRISPEQLEEAQN
HHHHHHHCCCCCEEEEEEEEECCCCEEEECCCCCCHHCCCHHHCCCCCCCCHHHHHHHHH
IAGKVTEALTGAGIWGVEFFLADDGVYFSELSPRPHDTGMVTLAGTQNLSEFELHARAVL
HHHHHHHHHHCCCEEEEEEEEECCCEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHEE
GLPVPDITLLRAGASAVILADRVGNNPSFDGLDAALAEPGSDIRIFGKPVMRPYRRMGVA
CCCCCCHHEEECCCCEEEEEECCCCCCCCCCCHHHHHCCCCCEEEECCHHHHHHHHHCEE
LMSGEKGSDVDELKRQAIANAEKVMIKCDETV
EECCCCCCCHHHHHHHHHCCCCEEEEEECCCC
>Mature Secondary Structure 
PKTIMLLGSGELGKEFVIAVKRLGQRVVAVDSYDDAPAQQVADRREVIDMLDGRALDAI
CCEEEEEECCCCCHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHH
VAKHRPDIIVPEIEAIRTERFYDYEKQGIQVVPSARAANFTMNRKAIRDLAARDLGLRTA
HHHCCCCEECCCHHHHHHHHHHCHHHCCEEEECCCCCCCEEECHHHHHHHHHHHHCCCCC
NYRYASSLEELRSAVGEVGLPCVVKPLMSSSGKGQSTVRSEAEIEAAWSYSQSGKRGDIA
CCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHH
EVIVEAFVPFHTEITLLTVTQKNGPTLFCPPIGHRQERGDYQESWQPCRISPEQLEEAQN
HHHHHHHCCCCCEEEEEEEEECCCCEEEECCCCCCHHCCCHHHCCCCCCCCHHHHHHHHH
IAGKVTEALTGAGIWGVEFFLADDGVYFSELSPRPHDTGMVTLAGTQNLSEFELHARAVL
HHHHHHHHHHCCCEEEEEEEEECCCEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHEE
GLPVPDITLLRAGASAVILADRVGNNPSFDGLDAALAEPGSDIRIFGKPVMRPYRRMGVA
CCCCCCHHEEECCCCEEEEEECCCCCCCCCCCHHHHHCCCCCEEEECCHHHHHHHHHCEE
LMSGEKGSDVDELKRQAIANAEKVMIKCDETV
EECCCCCCCHHHHHHHHHCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901