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The map label for this gene is cobD

Identifier: 21673768

GI number: 21673768

Start: 888722

End: 889681

Strand: Direct

Name: cobD

Synonym: CT0940

Alternate gene names: 21673768

Gene position: 888722-889681 (Clockwise)

Preceding gene: 21673767

Following gene: 21673769

Centisome position: 41.24

GC content: 62.29

Gene sequence:

>960_bases
GTGACCTGGTTGCTGATGCCAGCGGCGTTCCTGCTCGATCTGCTGCTTGGCGATCCGCAGTGGTTTCCGCATCCGGTGCG
GCTGGTCGGCAAGCTTGCGTCCGGCGCGGAGCGGTTGTTCCGGGGGATGCGGTTCCTGCCGCTCCGGCTGGCGGGCATTT
TGACGGCGCTGGTGGTCATCGGTACGACTGTCGTGACGGTGCTGGCGCTCGTGGTGGCGGCGTTTCTGCTTCATCCGCTT
GCTGGCGTCGTCGTATCGGTGCTTTTGCTCTATTTCGCGATTGCACCGCGCGATCTTTACAACCACGGATCGGCTGTGCG
GGATGCGCTTCGCGCGGGCGATCTCGAACTCGCCCGCCAGAAGGTGGCGATGATGGTTGGTCGCGATACCTCGATGCTCG
ACGAAGAGGGTGTGGCGAGGGCTGCTGTGGAGAGTGTGGCCGAGAATACATCGGATGGCGTGACCGCGCCGCTCTTTTAC
GGCTTGCTTTTCGGCCCTGCCGGGGCGTGGCTCTACAAGGCCTCGAACACGCTCGACTCGATGTTCGGTTACCGCAACGA
GCGTTACAGGGAGTTCGGCTGGGCGTCGGCGCGGTTCGATGATTTCATGAATTACCTGCCGTCGCGCCTGACGGTTTTGG
CGGTTGCGGGCGCGGCTTTTATTTTACGGCTCGATCCGGCGGGTGTTTTCCGTTCGGTGAAGCTGGGGGCGACGTTGCAC
GAAAGTCCCAATGCGGGCTATCCCGAAGCGGCATTTGCCGGAGCACTCGGTGTCACGCTTGGCGGCGAGCGGAGCTACGG
CGGAGTCACCAAAACCGTACCGACGCTCGGGCTTCGCGAAGGCGCGCTGGATGCCACGACGATCACAGGCGCCATGCGCC
TGATGTACGTTGCCTCGGTTCTGTTCATGGCCTGTGGCGCGCTGCTGCTTTTTATTCTTTCCAAGTCTCTACAATCGTAA

Upstream 100 bases:

>100_bases
GCATTCGCACCTCCGACGAAAACCGGCGTCTCATCGAGGCGCTGAACGATTTCGCCGAGTTGTCGAGGATCGCGAAAGCT
GCCGTCGAGGAGGCGGTGTC

Downstream 100 bases:

>100_bases
TCAGTTATGTCACAAAAACGGGTTCTTCTTTTCACAGGTAACGGCAAGGGCAAAAGCACGGCCGCGTTCGGAATGCTGTC
GCGTGCGCTCGGCCACGGTC

Product: cobalamin biosynthesis protein

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MTWLLMPAAFLLDLLLGDPQWFPHPVRLVGKLASGAERLFRGMRFLPLRLAGILTALVVIGTTVVTVLALVVAAFLLHPL
AGVVVSVLLLYFAIAPRDLYNHGSAVRDALRAGDLELARQKVAMMVGRDTSMLDEEGVARAAVESVAENTSDGVTAPLFY
GLLFGPAGAWLYKASNTLDSMFGYRNERYREFGWASARFDDFMNYLPSRLTVLAVAGAAFILRLDPAGVFRSVKLGATLH
ESPNAGYPEAAFAGALGVTLGGERSYGGVTKTVPTLGLREGALDATTITGAMRLMYVASVLFMACGALLLFILSKSLQS

Sequences:

>Translated_319_residues
MTWLLMPAAFLLDLLLGDPQWFPHPVRLVGKLASGAERLFRGMRFLPLRLAGILTALVVIGTTVVTVLALVVAAFLLHPL
AGVVVSVLLLYFAIAPRDLYNHGSAVRDALRAGDLELARQKVAMMVGRDTSMLDEEGVARAAVESVAENTSDGVTAPLFY
GLLFGPAGAWLYKASNTLDSMFGYRNERYREFGWASARFDDFMNYLPSRLTVLAVAGAAFILRLDPAGVFRSVKLGATLH
ESPNAGYPEAAFAGALGVTLGGERSYGGVTKTVPTLGLREGALDATTITGAMRLMYVASVLFMACGALLLFILSKSLQS
>Mature_318_residues
TWLLMPAAFLLDLLLGDPQWFPHPVRLVGKLASGAERLFRGMRFLPLRLAGILTALVVIGTTVVTVLALVVAAFLLHPLA
GVVVSVLLLYFAIAPRDLYNHGSAVRDALRAGDLELARQKVAMMVGRDTSMLDEEGVARAAVESVAENTSDGVTAPLFYG
LLFGPAGAWLYKASNTLDSMFGYRNERYREFGWASARFDDFMNYLPSRLTVLAVAGAAFILRLDPAGVFRSVKLGATLHE
SPNAGYPEAAFAGALGVTLGGERSYGGVTKTVPTLGLREGALDATTITGAMRLMYVASVLFMACGALLLFILSKSLQS

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBD_CHLTE (Q8KDV4)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_661833.1
- GeneID:   1007107
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT0940
- NMPDR:   fig|194439.1.peg.927
- TIGR:   CT0940
- HOGENOM:   HBG647131
- OMA:   IGDPWGW
- ProtClustDB:   PRK01209
- BioCyc:   CTEP194439:CT_0940-MONOMER
- HAMAP:   MF_00024
- InterPro:   IPR004485
- TIGRFAMs:   TIGR00380

Pfam domain/function: PF03186 CobD_Cbib

EC number: 6.3.1.10

Molecular weight: Translated: 34149; Mature: 34018

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xb0f268c)-; HASH(0xd899bb4)-; HASH(0xdcc7448)-; HASH(0xd4bcec8)-; HASH(0xdc39c78)-;

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTWLLMPAAFLLDLLLGDPQWFPHPVRLVGKLASGAERLFRGMRFLPLRLAGILTALVVI
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GTTVVTVLALVVAAFLLHPLAGVVVSVLLLYFAIAPRDLYNHGSAVRDALRAGDLELARQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHCCHHHHHH
KVAMMVGRDTSMLDEEGVARAAVESVAENTSDGVTAPLFYGLLFGPAGAWLYKASNTLDS
HHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
MFGYRNERYREFGWASARFDDFMNYLPSRLTVLAVAGAAFILRLDPAGVFRSVKLGATLH
HHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHCCCCC
ESPNAGYPEAAFAGALGVTLGGERSYGGVTKTVPTLGLREGALDATTITGAMRLMYVASV
CCCCCCCCHHHHHHHHHHEECCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
LFMACGALLLFILSKSLQS
HHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure 
TWLLMPAAFLLDLLLGDPQWFPHPVRLVGKLASGAERLFRGMRFLPLRLAGILTALVVI
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GTTVVTVLALVVAAFLLHPLAGVVVSVLLLYFAIAPRDLYNHGSAVRDALRAGDLELARQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHCCHHHHHH
KVAMMVGRDTSMLDEEGVARAAVESVAENTSDGVTAPLFYGLLFGPAGAWLYKASNTLDS
HHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
MFGYRNERYREFGWASARFDDFMNYLPSRLTVLAVAGAAFILRLDPAGVFRSVKLGATLH
HHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHCCCCC
ESPNAGYPEAAFAGALGVTLGGERSYGGVTKTVPTLGLREGALDATTITGAMRLMYVASV
CCCCCCCCHHHHHHHHHHEECCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
LFMACGALLLFILSKSLQS
HHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12093901