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The map label for this gene is trpA

Identifier: 21673370

GI number: 21673370

Start: 542208

End: 543011

Strand: Direct

Name: trpA

Synonym: CT0535

Alternate gene names: 21673370

Gene position: 542208-543011 (Clockwise)

Preceding gene: 21673369

Following gene: 21673371

Centisome position: 25.16

GC content: 60.45

Gene sequence:

>804_bases
ATGAAAGAGAACCGAATTACCCGGCTGGTGAAGCAGGACAAGAAGCTGCTCATCGCCTATTATATGCCTGAATTTCCCGT
GCCGGGCGCTACTTTGCCTGTGCTTGAAGCGTTGCAGGAGAGCGGGGTCGATCTCATCGAGCTCGGTATGCCCTACTCCG
ATCCCATTGGCGACGGGTCGGTGATCCAGGACGCGGCGCACAAGGCGATCAGCCATGGCGTGCATGTTGGCAGCATCTTC
GAGCTGGTCAGAAGAGCGCGCAATGGCGAGGGGTGCAAGAAGATCACCACGCCGATTCTGCTTATGGGTTACTGCAATCC
GCTCATCGCCTACGGCGGCGACTGTTTCATGGCCGATGCGGTGAAGGCGGGCGTGGATGGGTTGCTGATTCCCGACCTGC
CGCCCGAGGAGTCGGAGGATTTCCTCGAACGCGCCAAACACTTCGGCCTGTCGGTGATCTACCTCATTTCGCCGGTGACG
CCGCCTGACCGTATCGAGCTGATCGACAGCATGTCCACCGACTTTTCGTATTGCCTCGCGGTTAACGCCACCACCGGGAC
CGGCAAGCTCGACGTTGCAGGCATGGACGAGAAGATCGCCGAGTATCTAAAGCGCGTGCGGCAGCACACGAAAAAGAAGT
TCGTCGTCGGCTTTGGCATCAAGGATCGCGAGCGTGTGCGCAAGATGTGGGAGCTGGCCGACGGCGCGGTGGTCGGCTCG
GCGCTGCTGCAACACGTCGCCACAGCGAAAACCCCCGAAGAGACTGCGGAGCTGGCCGCCGGGTTCTGGAAATCGCTGCG
GTGA

Upstream 100 bases:

>100_bases
AGAACGTCTGGGGAAGCGTACTGGTACACATGATTACCATGTTTTCGATGGTGCTCGATATTTTTGTCATCCCCAATGTG
GTGCCTTCACTATTTCATCT

Downstream 100 bases:

>100_bases
TCCACGTGGCGGAATCCCTTCCAGTGCTCCGCCATTTCCAAGCACTTCGATTCTTTCAACCATGCAAGCATCGGGCAAAA
CCACACCGGCGGTGACGGTT

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MKENRITRLVKQDKKLLIAYYMPEFPVPGATLPVLEALQESGVDLIELGMPYSDPIGDGSVIQDAAHKAISHGVHVGSIF
ELVRRARNGEGCKKITTPILLMGYCNPLIAYGGDCFMADAVKAGVDGLLIPDLPPEESEDFLERAKHFGLSVIYLISPVT
PPDRIELIDSMSTDFSYCLAVNATTGTGKLDVAGMDEKIAEYLKRVRQHTKKKFVVGFGIKDRERVRKMWELADGAVVGS
ALLQHVATAKTPEETAELAAGFWKSLR

Sequences:

>Translated_267_residues
MKENRITRLVKQDKKLLIAYYMPEFPVPGATLPVLEALQESGVDLIELGMPYSDPIGDGSVIQDAAHKAISHGVHVGSIF
ELVRRARNGEGCKKITTPILLMGYCNPLIAYGGDCFMADAVKAGVDGLLIPDLPPEESEDFLERAKHFGLSVIYLISPVT
PPDRIELIDSMSTDFSYCLAVNATTGTGKLDVAGMDEKIAEYLKRVRQHTKKKFVVGFGIKDRERVRKMWELADGAVVGS
ALLQHVATAKTPEETAELAAGFWKSLR
>Mature_267_residues
MKENRITRLVKQDKKLLIAYYMPEFPVPGATLPVLEALQESGVDLIELGMPYSDPIGDGSVIQDAAHKAISHGVHVGSIF
ELVRRARNGEGCKKITTPILLMGYCNPLIAYGGDCFMADAVKAGVDGLLIPDLPPEESEDFLERAKHFGLSVIYLISPVT
PPDRIELIDSMSTDFSYCLAVNATTGTGKLDVAGMDEKIAEYLKRVRQHTKKKFVVGFGIKDRERVRKMWELADGAVVGS
ALLQHVATAKTPEETAELAAGFWKSLR

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=197, Percent_Identity=30.9644670050761, Blast_Score=100, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321412, Length=234, Percent_Identity=40.1709401709402, Blast_Score=176, Evalue=3e-45,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_CHLTE (Q8KEZ7)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_661435.1
- ProteinModelPortal:   Q8KEZ7
- SMR:   Q8KEZ7
- GeneID:   1006202
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT0535
- NMPDR:   fig|194439.1.peg.529
- TIGR:   CT0535
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- ProtClustDB:   PRK13111
- BioCyc:   CTEP194439:CT_0535-MONOMER
- BRENDA:   4.2.1.20
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 29260; Mature: 29260

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 47-47 ACT_SITE 58-58

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKENRITRLVKQDKKLLIAYYMPEFPVPGATLPVLEALQESGVDLIELGMPYSDPIGDGS
CCCHHHHHHHHHCHHEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCH
VIQDAAHKAISHGVHVGSIFELVRRARNGEGCKKITTPILLMGYCNPLIAYGGDCFMADA
HHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
VKAGVDGLLIPDLPPEESEDFLERAKHFGLSVIYLISPVTPPDRIELIDSMSTDFSYCLA
HHHCCCCEECCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEE
VNATTGTGKLDVAGMDEKIAEYLKRVRQHTKKKFVVGFGIKDRERVRKMWELADGAVVGS
EECCCCCCCEEECCCHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHCCHHHHH
ALLQHVATAKTPEETAELAAGFWKSLR
HHHHHHHHCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKENRITRLVKQDKKLLIAYYMPEFPVPGATLPVLEALQESGVDLIELGMPYSDPIGDGS
CCCHHHHHHHHHCHHEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCH
VIQDAAHKAISHGVHVGSIFELVRRARNGEGCKKITTPILLMGYCNPLIAYGGDCFMADA
HHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
VKAGVDGLLIPDLPPEESEDFLERAKHFGLSVIYLISPVTPPDRIELIDSMSTDFSYCLA
HHHCCCCEECCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEE
VNATTGTGKLDVAGMDEKIAEYLKRVRQHTKKKFVVGFGIKDRERVRKMWELADGAVVGS
EECCCCCCCEEECCCHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHCCHHHHH
ALLQHVATAKTPEETAELAAGFWKSLR
HHHHHHHHCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901