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The map label for this gene is hisH

Identifier: 21673311

GI number: 21673311

Start: 491866

End: 492471

Strand: Direct

Name: hisH

Synonym: CT0476

Alternate gene names: 21673311

Gene position: 491866-492471 (Clockwise)

Preceding gene: 21673304

Following gene: 21673312

Centisome position: 22.82

GC content: 51.65

Gene sequence:

>606_bases
ATGGTATTTATTGCCGACTATGGCGCCGGAAACCTCAGGTCGGTGCACAAGGCTTTCGATTATCTCGGTATCGAGGCAGT
CGTCAGCGACAAGGCATCCGAGATGAGCCGTTACGATAAAGTGCTTATTCCCGGTGTCGGCGCGTTTGGCCCGGCGATGG
AAGCGCTGAACCGCCAGGGATTCGACGAGGCGATCCGGGAGCATATCGACAAAGGCCGCAGTGTGCTCGGTATCTGCCTT
GGCATGCAGCTCTTTCTTTCAGAAAGCGAGGAGATGGGAGCGTATAAGGGCCTTGACATCGTGCCTGGCAAGGTGCTCCG
TTTTACCAGCAGTACCGACAAGATTCCGCAGATTGGCTGGAACTCTGTTGATTATTGCAAGGACTCGGTCCTTTTCCGCA
ATGTTCCAGATCAGTCGTATTTCTACTTCGTGCACTCTTACTATTGTGCTCCCGACGAGCCGGAAAGCGTGGCCGCGACC
ACCTTTTTCGCCGGGAAAAAATTTTGTTCGGCCATTGAGAAAAATGGTATTTTCGCGGTACAGTTTCACCCTGAAAAAAG
CTCCGAAGCAGGTTTGCAGGTGCTGAAAAATTTTGCAGAATTTTAA

Upstream 100 bases:

>100_bases
GAGAACTTCTTAAAAAAAAGACGTGTTGCCCCTTTTTCGAGGTTTGAACCGCTTTTTTCTCCATCCGGAGGCCATCAGCA
ACGTTTTAATCTTTAAGTTC

Downstream 100 bases:

>100_bases
GTCCTTGAGGTTATAAACGTTATGCTGATTATACCGGCTATAGATATTAAAGAAGGAAAGTGCGTCAGGCTGACCCGCGG
GGACTTCGCTCAGAAGAAAA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MVFIADYGAGNLRSVHKAFDYLGIEAVVSDKASEMSRYDKVLIPGVGAFGPAMEALNRQGFDEAIREHIDKGRSVLGICL
GMQLFLSESEEMGAYKGLDIVPGKVLRFTSSTDKIPQIGWNSVDYCKDSVLFRNVPDQSYFYFVHSYYCAPDEPESVAAT
TFFAGKKFCSAIEKNGIFAVQFHPEKSSEAGLQVLKNFAEF

Sequences:

>Translated_201_residues
MVFIADYGAGNLRSVHKAFDYLGIEAVVSDKASEMSRYDKVLIPGVGAFGPAMEALNRQGFDEAIREHIDKGRSVLGICL
GMQLFLSESEEMGAYKGLDIVPGKVLRFTSSTDKIPQIGWNSVDYCKDSVLFRNVPDQSYFYFVHSYYCAPDEPESVAAT
TFFAGKKFCSAIEKNGIFAVQFHPEKSSEAGLQVLKNFAEF
>Mature_201_residues
MVFIADYGAGNLRSVHKAFDYLGIEAVVSDKASEMSRYDKVLIPGVGAFGPAMEALNRQGFDEAIREHIDKGRSVLGICL
GMQLFLSESEEMGAYKGLDIVPGKVLRFTSSTDKIPQIGWNSVDYCKDSVLFRNVPDQSYFYFVHSYYCAPDEPESVAAT
TFFAGKKFCSAIEKNGIFAVQFHPEKSSEAGLQVLKNFAEF

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=200, Percent_Identity=38, Blast_Score=126, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6319725, Length=208, Percent_Identity=35.0961538461538, Blast_Score=131, Evalue=7e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_CHLTE (Q8KF56)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_661376.1
- ProteinModelPortal:   Q8KF56
- SMR:   Q8KF56
- GeneID:   1008215
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT0476
- NMPDR:   fig|194439.1.peg.470
- TIGR:   CT0476
- HOGENOM:   HBG292341
- OMA:   WNEINIV
- ProtClustDB:   PRK13181
- BioCyc:   CTEP194439:CT_0476-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 22259; Mature: 22259

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 79-79 ACT_SITE 183-183 ACT_SITE 185-185

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFIADYGAGNLRSVHKAFDYLGIEAVVSDKASEMSRYDKVLIPGVGAFGPAMEALNRQG
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCHHHHHHHHCCCC
FDEAIREHIDKGRSVLGICLGMQLFLSESEEMGAYKGLDIVPGKVLRFTSSTDKIPQIGW
HHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCEEEECCCCCCCCCCCC
NSVDYCKDSVLFRNVPDQSYFYFVHSYYCAPDEPESVAATTFFAGKKFCSAIEKNGIFAV
CCHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEE
QFHPEKSSEAGLQVLKNFAEF
EECCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MVFIADYGAGNLRSVHKAFDYLGIEAVVSDKASEMSRYDKVLIPGVGAFGPAMEALNRQG
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCHHHHHHHHCCCC
FDEAIREHIDKGRSVLGICLGMQLFLSESEEMGAYKGLDIVPGKVLRFTSSTDKIPQIGW
HHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCEEEECCCCCCCCCCCC
NSVDYCKDSVLFRNVPDQSYFYFVHSYYCAPDEPESVAATTFFAGKKFCSAIEKNGIFAV
CCHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEE
QFHPEKSSEAGLQVLKNFAEF
EECCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901