Click here to switch to the map view.

The map label for this gene is hisA [H]

Identifier: 21673312

GI number: 21673312

Start: 492493

End: 493275

Strand: Direct

Name: hisA [H]

Synonym: CT0477

Alternate gene names: 21673312

Gene position: 492493-493275 (Clockwise)

Preceding gene: 21673311

Following gene: 21673313

Centisome position: 22.85

GC content: 56.58

Gene sequence:

>783_bases
ATGCTGATTATACCGGCTATAGATATTAAAGAAGGAAAGTGCGTCAGGCTGACCCGCGGGGACTTCGCTCAGAAGAAAAT
CTATCTTGACAATCCGTGCGACATGGCGGTCATCTGGCGGAAGCAGAACGCCAAGATGATCCATGTGGTCGATCTCGACG
CAGCCCTGACTGGTGAGACGGTCAACTTCGAGAGGATTCGCGAGATCGTCAATGTGCTCGATATTCCGATCCAGGTCGGC
GGTGGCATCCGCTCTGTCGAGGCGGTTGAAAAGTACCTTGACATTGGCGTCAGCCGTGTGGTTATTGGCTCTGCGGCGGT
TACCAATCCCGGCCTCATCGCCGATCTCCTGAAGAAATATCGTCCTTCGCAGATCGTCGTCGGCATCGATGCCGAGCACG
GCGTGCCCAAAATCAAGGGGTGGACCGAGAGCAGCAATATGCAGGATTACGAGCTTGCTGGCGAAATGAAGAAGCTCGGC
GTCGAGCGCATCATTTACACCGACATCACCCGTGACGGCATGTTGCAGGGGGTCGGCTACGAAACCACCAAACGCTTTGC
CGAGAAGGCGGGCATGAAGGTGACCGCGTCTGGTGGCGCGACCACTTCCGATGACCTGCACAAGCTCCGCTCGCTCGAAA
AGTACGGCGTCGATTCGGTCATTATCGGCAAGGCGCTTTACGAGTGCAATTTTCCGTGCCAGGAGCTGTGGTACGCCTAC
GAGCAGGGGCTTGGCATCGACGGCGAATTTTCCACGGCCCGCAAGAAGGAGTGCTGCTCCTGA

Upstream 100 bases:

>100_bases
TATTTTCGCGGTACAGTTTCACCCTGAAAAAAGCTCCGAAGCAGGTTTGCAGGTGCTGAAAAATTTTGCAGAATTTTAAG
TCCTTGAGGTTATAAACGTT

Downstream 100 bases:

>100_bases
CGGCGGCCGCATCGCGCTTGCAACCATCGACAGTCACCGTGCAGGAACAACGCCAACAACTTCTCCGGTCGCTTGAAGCC
CTGATCTTCTCCTCGGAAGA

Product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MLIIPAIDIKEGKCVRLTRGDFAQKKIYLDNPCDMAVIWRKQNAKMIHVVDLDAALTGETVNFERIREIVNVLDIPIQVG
GGIRSVEAVEKYLDIGVSRVVIGSAAVTNPGLIADLLKKYRPSQIVVGIDAEHGVPKIKGWTESSNMQDYELAGEMKKLG
VERIIYTDITRDGMLQGVGYETTKRFAEKAGMKVTASGGATTSDDLHKLRSLEKYGVDSVIIGKALYECNFPCQELWYAY
EQGLGIDGEFSTARKKECCS

Sequences:

>Translated_260_residues
MLIIPAIDIKEGKCVRLTRGDFAQKKIYLDNPCDMAVIWRKQNAKMIHVVDLDAALTGETVNFERIREIVNVLDIPIQVG
GGIRSVEAVEKYLDIGVSRVVIGSAAVTNPGLIADLLKKYRPSQIVVGIDAEHGVPKIKGWTESSNMQDYELAGEMKKLG
VERIIYTDITRDGMLQGVGYETTKRFAEKAGMKVTASGGATTSDDLHKLRSLEKYGVDSVIIGKALYECNFPCQELWYAY
EQGLGIDGEFSTARKKECCS
>Mature_260_residues
MLIIPAIDIKEGKCVRLTRGDFAQKKIYLDNPCDMAVIWRKQNAKMIHVVDLDAALTGETVNFERIREIVNVLDIPIQVG
GGIRSVEAVEKYLDIGVSRVVIGSAAVTNPGLIADLLKKYRPSQIVVGIDAEHGVPKIKGWTESSNMQDYELAGEMKKLG
VERIIYTDITRDGMLQGVGYETTKRFAEKAGMKVTASGGATTSDDLHKLRSLEKYGVDSVIIGKALYECNFPCQELWYAY
EQGLGIDGEFSTARKKECCS

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI87082028, Length=243, Percent_Identity=32.9218106995885, Blast_Score=127, Evalue=9e-31,
Organism=Escherichia coli, GI1788336, Length=255, Percent_Identity=28.2352941176471, Blast_Score=85, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: =5.3.1.16 [H]

Molecular weight: Translated: 28712; Mature: 28712

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIIPAIDIKEGKCVRLTRGDFAQKKIYLDNPCDMAVIWRKQNAKMIHVVDLDAALTGET
CEEECCEECCCCCEEEEECCCCCCCEEEECCCCCEEEEEECCCCCEEEEEECCCCCCCCC
VNFERIREIVNVLDIPIQVGGGIRSVEAVEKYLDIGVSRVVIGSAAVTNPGLIADLLKKY
CCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCCHHHEEECCCCCCCCCHHHHHHHHC
RPSQIVVGIDAEHGVPKIKGWTESSNMQDYELAGEMKKLGVERIIYTDITRDGMLQGVGY
CCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHEEEECCCCCCCCCCCCH
ETTKRFAEKAGMKVTASGGATTSDDLHKLRSLEKYGVDSVIIGKALYECNFPCQELWYAY
HHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHCCCCHHHHHHHH
EQGLGIDGEFSTARKKECCS
HHCCCCCCCCCHHHHHHCCC
>Mature Secondary Structure
MLIIPAIDIKEGKCVRLTRGDFAQKKIYLDNPCDMAVIWRKQNAKMIHVVDLDAALTGET
CEEECCEECCCCCEEEEECCCCCCCEEEECCCCCEEEEEECCCCCEEEEEECCCCCCCCC
VNFERIREIVNVLDIPIQVGGGIRSVEAVEKYLDIGVSRVVIGSAAVTNPGLIADLLKKY
CCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCCHHHEEECCCCCCCCCHHHHHHHHC
RPSQIVVGIDAEHGVPKIKGWTESSNMQDYELAGEMKKLGVERIIYTDITRDGMLQGVGY
CCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHEEEECCCCCCCCCCCCH
ETTKRFAEKAGMKVTASGGATTSDDLHKLRSLEKYGVDSVIIGKALYECNFPCQELWYAY
HHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHCCCCHHHHHHHH
EQGLGIDGEFSTARKKECCS
HHCCCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA