Click here to switch to the map view.

The map label for this gene is pdxJ

Identifier: 21673170

GI number: 21673170

Start: 347309

End: 348022

Strand: Reverse

Name: pdxJ

Synonym: CT0331

Alternate gene names: 21673170

Gene position: 348022-347309 (Counterclockwise)

Preceding gene: 21673176

Following gene: 21673169

Centisome position: 16.15

GC content: 61.62

Gene sequence:

>714_bases
ATGAGACTTGCCGTCAATATTGACCATATTGCCACCCTGCGCAACGCTCGCAACGAAGGGCATCCCGATCCCGTCGAAGC
CGCTCTGCTCGCCGAAAAGCACGGCGCGGCAGGTATCGTCTGCCACCTGCGCGAAGACCGCCGCCACATCAAGGACGACG
ACCTCGCACGGCTGCGAGAGGAGATCACCACCAAGCTCGACCTCGAAATGGCCATGACCGACGAGATGCAGAAAATCGCG
CTCTCGGTCAGGCCCGACCTGGTGACGCTTGTGCCCGAAAAGCGCGAGGAGCTGACCACCGAGGGTGGATTCGCCATCCA
GAAGCACTTCACCCGCCTCACGGAATTCGTCAAACCGTTGCGGGACAAGGAGATAGGCGTCAGCGTCTTCATCGAGCCGG
AAGAGGAGGCCATCGAACTCGCTGCCGAAGCCGGGGCGAACATCGTCGAATTCCACACCGGTACCTACTCGCTCTGCACC
AGCGACAAACAGACGGCCTACGAGCTGGAACGCATCCGCAATTCGGCGCGCATCGCCCGCGAAATGGGGCTGACCGTGGT
TGCCGGGCACGGCCTGAGCGTACTGAACATCGCTCCGTTCAAGGAGCTGCACGACATCGAGGAGGTGAGCATCGGCCACG
CCATCATCTCACGCGCCGTGTTCATCGGCCTTCCGGCAGCCATCCAGGAAATCCTCGATCTCATCCGCCGGTAA

Upstream 100 bases:

>100_bases
CCTACCGCTGATTTTACGTTTTGTTTCTTTTGTTTTATCTATCTTCTCAAATCACCGGGACAATTCCCCAAGCATGAACA
TCGCATAAGAGACTACAACC

Downstream 100 bases:

>100_bases
CGCTATGGAACCGAAACATCCCGAAATCACCATCGTTCTCGCCACCGGCAACAAGGACAAGGTTCGCGAGCTGAAGCCGG
TACTCGAAGCTCTCGCCTCC

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MRLAVNIDHIATLRNARNEGHPDPVEAALLAEKHGAAGIVCHLREDRRHIKDDDLARLREEITTKLDLEMAMTDEMQKIA
LSVRPDLVTLVPEKREELTTEGGFAIQKHFTRLTEFVKPLRDKEIGVSVFIEPEEEAIELAAEAGANIVEFHTGTYSLCT
SDKQTAYELERIRNSARIAREMGLTVVAGHGLSVLNIAPFKELHDIEEVSIGHAIISRAVFIGLPAAIQEILDLIRR

Sequences:

>Translated_237_residues
MRLAVNIDHIATLRNARNEGHPDPVEAALLAEKHGAAGIVCHLREDRRHIKDDDLARLREEITTKLDLEMAMTDEMQKIA
LSVRPDLVTLVPEKREELTTEGGFAIQKHFTRLTEFVKPLRDKEIGVSVFIEPEEEAIELAAEAGANIVEFHTGTYSLCT
SDKQTAYELERIRNSARIAREMGLTVVAGHGLSVLNIAPFKELHDIEEVSIGHAIISRAVFIGLPAAIQEILDLIRR
>Mature_237_residues
MRLAVNIDHIATLRNARNEGHPDPVEAALLAEKHGAAGIVCHLREDRRHIKDDDLARLREEITTKLDLEMAMTDEMQKIA
LSVRPDLVTLVPEKREELTTEGGFAIQKHFTRLTEFVKPLRDKEIGVSVFIEPEEEAIELAAEAGANIVEFHTGTYSLCT
SDKQTAYELERIRNSARIAREMGLTVVAGHGLSVLNIAPFKELHDIEEVSIGHAIISRAVFIGLPAAIQEILDLIRR

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family

Homologues:

Organism=Escherichia coli, GI1788917, Length=233, Percent_Identity=49.3562231759657, Blast_Score=224, Evalue=4e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXJ_CHLTE (Q8KFJ5)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_661235.1
- ProteinModelPortal:   Q8KFJ5
- SMR:   Q8KFJ5
- GeneID:   1007993
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT0331
- NMPDR:   fig|194439.1.peg.329
- TIGR:   CT0331
- HOGENOM:   HBG299314
- OMA:   AGHGLNY
- ProtClustDB:   PRK05265
- BioCyc:   CTEP194439:CT_0331-MONOMER
- BRENDA:   2.6.99.2
- GO:   GO:0005737
- HAMAP:   MF_00279
- InterPro:   IPR013785
- InterPro:   IPR004569
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00559

Pfam domain/function: PF03740 PdxJ; SSF63892 PyrdxlP_synth_PdxJ

EC number: =2.6.99.2

Molecular weight: Translated: 26451; Mature: 26451

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: ACT_SITE 42-42 ACT_SITE 69-69 ACT_SITE 190-190 BINDING 6-6 BINDING 17-17 BINDING 44-44 BINDING 49-49 BINDING 99-99 BINDING 191-191

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLAVNIDHIATLRNARNEGHPDPVEAALLAEKHGAAGIVCHLREDRRHIKDDDLARLRE
CEEEEEHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEEEEEHHHCCCCCHHHHHHHH
EITTKLDLEMAMTDEMQKIALSVRPDLVTLVPEKREELTTEGGFAIQKHFTRLTEFVKPL
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHCCCCCHHHHHHHHHHHHHHHHH
RDKEIGVSVFIEPEEEAIELAAEAGANIVEFHTGTYSLCTSDKQTAYELERIRNSARIAR
CCCCCCEEEEECCHHHHHHHHHHCCCCEEEEECCCEEEECCCHHHHHHHHHHHHHHHHHH
EMGLTVVAGHGLSVLNIAPFKELHDIEEVSIGHAIISRAVFIGLPAAIQEILDLIRR
HCCCEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCC
>Mature Secondary Structure
MRLAVNIDHIATLRNARNEGHPDPVEAALLAEKHGAAGIVCHLREDRRHIKDDDLARLRE
CEEEEEHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEEEEEHHHCCCCCHHHHHHHH
EITTKLDLEMAMTDEMQKIALSVRPDLVTLVPEKREELTTEGGFAIQKHFTRLTEFVKPL
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHCCCCCHHHHHHHHHHHHHHHHH
RDKEIGVSVFIEPEEEAIELAAEAGANIVEFHTGTYSLCTSDKQTAYELERIRNSARIAR
CCCCCCEEEEECCHHHHHHHHHHCCCCEEEEECCCEEEECCCHHHHHHHHHHHHHHHHHH
EMGLTVVAGHGLSVLNIAPFKELHDIEEVSIGHAIISRAVFIGLPAAIQEILDLIRR
HCCCEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901