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The map label for this gene is ruvA

Identifier: 21673101

GI number: 21673101

Start: 272059

End: 272664

Strand: Direct

Name: ruvA

Synonym: CT0262

Alternate gene names: 21673101

Gene position: 272059-272664 (Clockwise)

Preceding gene: 21673100

Following gene: 21673102

Centisome position: 12.62

GC content: 56.93

Gene sequence:

>606_bases
ATGTTCGCATTTTTGAGAGGTGAGCTGGTAACAGTCTCCCGTGAGGAGGCGGTTGTCGAGGTATCCGGCATCGGGTACCT
GCTGCACATCTCGTCCGGCACGAGCCGCCGCCTGCCCCCGGAGGGGAGTCAGGTTCGTCTCTTTACCCATCACTACGTTC
GCGAGGATGCTCAGCAACTTTTCGGCTTTCTCGACGAAGAAGAGCTTCAGCTTTTCCGCTTGTTGCTTACCATCGGTGGA
GTCGGACCGAAGCTGGCGATGGCTGTGCTGTCGGGTCTGAGCGTTGGCGAAATCCAGGAAGCGATTGTCGCCAATCGTCC
GGAAACCCTTTACGGCATTACCGGTGTGGGCAAAAAAACCGCCTCCCGTATCATTCTCGAACTGAGGGACAAGATTCTCA
AAATCCAGCCAGCGGCAAGCGGCAAAACGGCGGGTGCGCCTCAAGCTCTTCAACTGAACGAAGATGCCCTCGCGGCGCTC
ATGACGCTCGGTTTTCCGAAGCCAGCGGCTCAGAAAGCCATTTCGGGCATTCTGGAGACTTCTCCCGGCTTGTCGGTCGA
AGAGGTTGTCAGGGCCGCGCTTATCGCTATTCACAACAACTTCTGA

Upstream 100 bases:

>100_bases
GCGAGCGGGCGATGTTTCATATTCAGTCATACCCGGCCAGCAGCTGGCGCTTTGAAGGCGCTAAGAGTTCGCAAACCGGG
TATTGTCCATTCCATTCATC

Downstream 100 bases:

>100_bases
GTCACTGTGGATTACCAGCAAGCTATCGATTTCCTGTTTCCCCTTCATCGTTTTGGCATCAAACCCGGCCTTGAACGTAT
CGAAGCGCTGCTCGACGTGC

Product: Holliday junction DNA helicase RuvA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MFAFLRGELVTVSREEAVVEVSGIGYLLHISSGTSRRLPPEGSQVRLFTHHYVREDAQQLFGFLDEEELQLFRLLLTIGG
VGPKLAMAVLSGLSVGEIQEAIVANRPETLYGITGVGKKTASRIILELRDKILKIQPAASGKTAGAPQALQLNEDALAAL
MTLGFPKPAAQKAISGILETSPGLSVEEVVRAALIAIHNNF

Sequences:

>Translated_201_residues
MFAFLRGELVTVSREEAVVEVSGIGYLLHISSGTSRRLPPEGSQVRLFTHHYVREDAQQLFGFLDEEELQLFRLLLTIGG
VGPKLAMAVLSGLSVGEIQEAIVANRPETLYGITGVGKKTASRIILELRDKILKIQPAASGKTAGAPQALQLNEDALAAL
MTLGFPKPAAQKAISGILETSPGLSVEEVVRAALIAIHNNF
>Mature_201_residues
MFAFLRGELVTVSREEAVVEVSGIGYLLHISSGTSRRLPPEGSQVRLFTHHYVREDAQQLFGFLDEEELQLFRLLLTIGG
VGPKLAMAVLSGLSVGEIQEAIVANRPETLYGITGVGKKTASRIILELRDKILKIQPAASGKTAGAPQALQLNEDALAAL
MTLGFPKPAAQKAISGILETSPGLSVEEVVRAALIAIHNNF

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family

Homologues:

Organism=Escherichia coli, GI1788168, Length=203, Percent_Identity=35.9605911330049, Blast_Score=118, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVA_CHLTE (Q8KFR0)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_661166.1
- ProteinModelPortal:   Q8KFR0
- SMR:   Q8KFR0
- GeneID:   1007779
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT0262
- NMPDR:   fig|194439.1.peg.260
- TIGR:   CT0262
- HOGENOM:   HBG635309
- OMA:   LSIETYV
- ProtClustDB:   PRK00116
- BioCyc:   CTEP194439:CT_0262-MONOMER
- HAMAP:   MF_00031
- InterPro:   IPR011114
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00278
- TIGRFAMs:   TIGR00084

Pfam domain/function: PF07499 RuvA_C; PF01330 RuvA_N; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like; SSF46929 RuvA_C-like

EC number: =3.6.4.12

Molecular weight: Translated: 21540; Mature: 21540

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFAFLRGELVTVSREEAVVEVSGIGYLLHISSGTSRRLPPEGSQVRLFTHHYVREDAQQL
CCEEECCCEEEEECCCEEEEEECCEEEEEECCCCCCCCCCCCCEEEEHHHHHHHHHHHHH
FGFLDEEELQLFRLLLTIGGVGPKLAMAVLSGLSVGEIQEAIVANRPETLYGITGVGKKT
HCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEECCCCHHH
ASRIILELRDKILKIQPAASGKTAGAPQALQLNEDALAALMTLGFPKPAAQKAISGILET
HHHHHHHHHHHHEEECCCCCCCCCCCCCEEEECHHHHHHHHHHCCCCHHHHHHHHHHHHC
SPGLSVEEVVRAALIAIHNNF
CCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFAFLRGELVTVSREEAVVEVSGIGYLLHISSGTSRRLPPEGSQVRLFTHHYVREDAQQL
CCEEECCCEEEEECCCEEEEEECCEEEEEECCCCCCCCCCCCCEEEEHHHHHHHHHHHHH
FGFLDEEELQLFRLLLTIGGVGPKLAMAVLSGLSVGEIQEAIVANRPETLYGITGVGKKT
HCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEECCCCHHH
ASRIILELRDKILKIQPAASGKTAGAPQALQLNEDALAALMTLGFPKPAAQKAISGILET
HHHHHHHHHHHHEEECCCCCCCCCCCCCEEEECHHHHHHHHHHCCCCHHHHHHHHHHHHC
SPGLSVEEVVRAALIAIHNNF
CCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901