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The map label for this gene is pcm

Identifier: 21673043

GI number: 21673043

Start: 213432

End: 214073

Strand: Direct

Name: pcm

Synonym: CT0202

Alternate gene names: 21673043

Gene position: 213432-214073 (Clockwise)

Preceding gene: 21673041

Following gene: 21673044

Centisome position: 9.9

GC content: 61.99

Gene sequence:

>642_bases
ATGGCTCGGGAGCGCCAGGAGATGGTGGTAGAGCTCAAGCGTTACGGTATCAGCAACGCCCGAGTGCTCGATGCGTTTCT
GACGGTCAGGCGTCATCTCTTTGTCGATGCACAGAGCCGGCCCTACGCCTACAGCGACAATGCTATGCCGATCGGATTCG
GCCAGACCATTTCGCAGCCCTATACGGTGGCGTACATGACTTCGCTGCTGGTGGAGCGCGTTCCATCCGGCAAGGTGCTT
GAAATCGGTACCGGTTCGGGCTACCAGGCGGCTATTCTCGCCGAGCTTGGCTATCGTGTCTATACCATCGAGCGGATTGC
CGGGTTGTACGCAGCGGCGGGGCGCGTGCTCGATGCGCTTGGTTTGCCCGTTCACCCGCGTCTCGGTGATGGAACGCTCG
GCTGGCCGGAGGAGGCGCCGTTCGACGGCATCATCGTGACCGCCGCCGCGCCGCGCGAGCCGCATACGCTCATGAGCCAG
CTTGCCGAAGGCGGCGTGCTCGTTGTGCCGATTGGCGACCTCGGTTCACAGCAGATGACCGTCATTCGGCGCAGGGGCGA
GCGGTTCGAGCATGAGATTTTTCACAACTTCGCCTTCGTGCCGCTCTGCGGGCGTGAAGGTTGGGCAGACAACAACGAAT
AA

Upstream 100 bases:

>100_bases
TTTTATCAGATGCAATAGTAGCGAATCTTCGGCGGATTCCATGACGGTGTGCTGACTGGAACAGGTTGGGAAACGTATGA
GGATGCAACAGGATGACGAC

Downstream 100 bases:

>100_bases
ACAGTCAAAGACAGAAAAGAGAAGTTATGGCACTAATGAGCAAGTTGAGGGATAAAACGCACATCGTGTTGTTTGTCCTC
GTGGCGGCGTTCCTTGCCCT

Product: protein-L-isoaspartate (D-aspartate) O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MARERQEMVVELKRYGISNARVLDAFLTVRRHLFVDAQSRPYAYSDNAMPIGFGQTISQPYTVAYMTSLLVERVPSGKVL
EIGTGSGYQAAILAELGYRVYTIERIAGLYAAAGRVLDALGLPVHPRLGDGTLGWPEEAPFDGIIVTAAAPREPHTLMSQ
LAEGGVLVVPIGDLGSQQMTVIRRRGERFEHEIFHNFAFVPLCGREGWADNNE

Sequences:

>Translated_213_residues
MARERQEMVVELKRYGISNARVLDAFLTVRRHLFVDAQSRPYAYSDNAMPIGFGQTISQPYTVAYMTSLLVERVPSGKVL
EIGTGSGYQAAILAELGYRVYTIERIAGLYAAAGRVLDALGLPVHPRLGDGTLGWPEEAPFDGIIVTAAAPREPHTLMSQ
LAEGGVLVVPIGDLGSQQMTVIRRRGERFEHEIFHNFAFVPLCGREGWADNNE
>Mature_212_residues
ARERQEMVVELKRYGISNARVLDAFLTVRRHLFVDAQSRPYAYSDNAMPIGFGQTISQPYTVAYMTSLLVERVPSGKVLE
IGTGSGYQAAILAELGYRVYTIERIAGLYAAAGRVLDALGLPVHPRLGDGTLGWPEEAPFDGIIVTAAAPREPHTLMSQL
AEGGVLVVPIGDLGSQQMTVIRRRGERFEHEIFHNFAFVPLCGREGWADNNE

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=216, Percent_Identity=32.4074074074074, Blast_Score=86, Evalue=2e-17,
Organism=Escherichia coli, GI1789100, Length=207, Percent_Identity=49.2753623188406, Blast_Score=169, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI71983477, Length=219, Percent_Identity=30.1369863013699, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI17981723, Length=219, Percent_Identity=31.9634703196347, Blast_Score=81, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT_CHLTE (Q8KFW8)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_661108.1
- HSSP:   Q8TZR3
- ProteinModelPortal:   Q8KFW8
- SMR:   Q8KFW8
- GeneID:   1007645
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT0202
- NMPDR:   fig|194439.1.peg.202
- TIGR:   CT0202
- HOGENOM:   HBG699907
- OMA:   YMVARMS
- ProtClustDB:   CLSK637215
- BioCyc:   CTEP194439:CT_0202-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 23447; Mature: 23316

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 58-58

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARERQEMVVELKRYGISNARVLDAFLTVRRHLFVDAQSRPYAYSDNAMPIGFGQTISQP
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEECCCCCCEEECCCCCEECCCCCCCCC
YTVAYMTSLLVERVPSGKVLEIGTGSGYQAAILAELGYRVYTIERIAGLYAAAGRVLDAL
HHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHH
GLPVHPRLGDGTLGWPEEAPFDGIIVTAAAPREPHTLMSQLAEGGVLVVPIGDLGSQQMT
CCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEECCCCCCHHHH
VIRRRGERFEHEIFHNFAFVPLCGREGWADNNE
HHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
ARERQEMVVELKRYGISNARVLDAFLTVRRHLFVDAQSRPYAYSDNAMPIGFGQTISQP
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEECCCCCCEEECCCCCEECCCCCCCCC
YTVAYMTSLLVERVPSGKVLEIGTGSGYQAAILAELGYRVYTIERIAGLYAAAGRVLDAL
HHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHH
GLPVHPRLGDGTLGWPEEAPFDGIIVTAAAPREPHTLMSQLAEGGVLVVPIGDLGSQQMT
CCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEECCCCCCHHHH
VIRRRGERFEHEIFHNFAFVPLCGREGWADNNE
HHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901