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The map label for this gene is ponA [H]

Identifier: 21673017

GI number: 21673017

Start: 184492

End: 186783

Strand: Reverse

Name: ponA [H]

Synonym: CT0176

Alternate gene names: 21673017

Gene position: 186783-184492 (Counterclockwise)

Preceding gene: 21673018

Following gene: 21673016

Centisome position: 8.67

GC content: 60.38

Gene sequence:

>2292_bases
GTGAGTAAAAAATTTGTTATGAAAATTGCCGGTCTTCTTCTTGTCGTTCTTCTTGGAGCCGGCGCTGTGTTCGGACTCGA
TCTTTTCAAAGGCCTTCCAAGCGTTCAGGAGCTTGAAAATCCCAAGCCGGAACTGGCCTCGCTGGTCTATTCGGAAGACG
GCAAACTCCTGCACAAGTTTTTCCTGAAAAACAGAACCTTCGTACCGCTCAGGTCAATATCCAGGTGGGCGCCTGCGGCA
CTCATCGCCACCGAGGATGTGACCTTCTACCAGCACTGGGGCGTTGACCTCCGGCGTCTGGCCATCGCCTTAGGCGAAAA
TATCATCAAGGGTCGCACCCGCTGGCAAGGCGCGAGCACCATCACGCAGCAGCTCGCCAAAAACCTTTTCCTGACCCAGG
AGCGCACCCTGAGCCGCAAGGCCAAGGAGCTCGTCACGGCGGTTCAGCTCGAACGCACCTATACCAAAAACGAGATTCTG
GCCCTCTATCTGAACACCGTCTACTTCGGATCGGGCGCTTACGGCATCGAGTCGGCAGCCCAGACCTATTTCGGCAAACC
GGCCAGCCAGCTCACCATTCCCGAGAGCGCCACGTTGATCGCAACGCTGAAAAATCCGACGGCCTACGATCCGTCCAAAG
ACCCTTCGTCGTCGCTGGCTCGGCGCAACCTGATTATCGGGCTGATGGAAAAGGCGGGGTTCATCACCCACGCCCAGGCC
GTCAGGGCAAAGGCCACGCCGCTGGTGCTGCACTACACGCCCGTCACCCAACAGGGCCTGGCCCCCTACTTCACCGAGTA
CATCCGGCAGACCATCAAATCGTCGTCCATGCTTGAAGGCGTCAACGTTTACCGCGACGGTCTGACCATCCAGACCACGC
TCGACAGCCGGATGCAGCAATACGCGCAACAGGCCATCGGCGAACAGGCGGCGGCCCTGCAAGCGCAGTTTGACCGGAGC
TGGCGCTGCCCCGAGTCGCTCAAGATCCAGTTCATCAAGGAGAGCGCCCGGTACAAGGAGATGGTAAACGAGGAGGGTGT
TCCCGCAAACACGGCGCTTGCCCGGCTGAAGGCCGATAGCGCCTGGCTGAACAATCTGCTTCACCAGAAAACGCGGGTGC
AGATGGCGCTCATCGCCCTTGACCCGTCGAACGGCCATGTCAAAGCGTGGGTTGGCGGCACCAATTTCGGGCCAGATGAC
TATCGCTACCAGTTCGACCATGTTTGGCAGGCCAAGCGTCAGCCCGGTTCGGCCTTCAAGCCCTTCGTCTATGCGGCTGC
CATCGACCAGGGCATACCGGCAAACTACAGGATTCTTGACCAGCCCCTCGCGCTGAAAAGCGGCAACGGCGGCGTCTGGG
TAGCCCGGAACGCCGAGGGCGGCTCCGGCGGCATGACGACGCTGCGCGACGCACTGGCCCACTCGCTCAACCAGGTCACC
GTCAGGCTCGCCTACGAGTTCCTGAGTCCTTCGGAAATCATCAGTTACGCCCGTCGCATGGGCATCACCTCCCCCATGCA
GCCCAACCTGTCGATCGCGCTCGGCACATCAGAGGTCTCGCCGCTCGAACTGGCCAGGGCATATACCACTTTTGCCAATA
ACGGCGCCTGGTGCGACGTGCTTCCCGTAACCAAAGTGCTCGACAAGCACGGCCGGGTGATTGCCGAACACCAGCCCAGC
AGCCATCTGGGACTCGATCCGGCGACCAACTACGTCATGGTCACCATGCTCAAGGATGTGATCGACCGAGGCACGGGTAT
CGCCGCCAGGACGCGCTACGGCCTCGACATGGAGGTGGCCGGTAAAACCGGCACCACCCAGAGCCAGCGCGACGCCTGGT
TCGCCGGGTTCACCCCAAAGCTCGTAGCCGTAGTCTGGGCGGGATTCGATGACGAACGGATTCATTTCACCTCGATGGAG
TATGGACAGGGCGCCAGAGCCGCGCTTCCGGCGTGGGCGAACTTCATGAAAAAGTGTTACTCCGATCCTTCGCTCAAGCT
TGAAAAAACCTACTTCCCGATGCCCGACAACGTTATTGCCGTGCCGATTGCAGGCTCATCGACCGAGCCGTCCGACGTGC
TAAACCGTAACGTCTCTGTCGAATATTTCACGCCAAAAGGCTACGCACGCTACCAGGCTGGCGATTTCAACAGAGCGCCG
CAACCGGTCAATTCCGGCGCGGTTGGCAATCCGGACAGCACCGGCGCGCAGCAACCGCCTGTCCCGACAGCACCGACAGC
ACCGACAGCACCGGCAAGCACCGACAGCACCGGCAGCCGCGCCCGCAGATAA

Upstream 100 bases:

>100_bases
TCTGGCCGACCGGGTGCAGATTCAATGAAATGATTCAACGGAAAAAATGTTCCTGTTGAAAACAGACGCTTATGATTCAC
CATTCTGAACCTTTGTCAAC

Downstream 100 bases:

>100_bases
TTTTTAAACAATACTTTACCGGTTTCACATCAACCATGGCAACATCCCTGCAATCGGTCATCGTCCTCGATTTCGGTTCA
CAGTACACTCAGCTCATCGC

Product: penicillin-binding protein 1

Products: NA

Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 763; Mature: 762

Protein sequence:

>763_residues
MSKKFVMKIAGLLLVVLLGAGAVFGLDLFKGLPSVQELENPKPELASLVYSEDGKLLHKFFLKNRTFVPLRSISRWAPAA
LIATEDVTFYQHWGVDLRRLAIALGENIIKGRTRWQGASTITQQLAKNLFLTQERTLSRKAKELVTAVQLERTYTKNEIL
ALYLNTVYFGSGAYGIESAAQTYFGKPASQLTIPESATLIATLKNPTAYDPSKDPSSSLARRNLIIGLMEKAGFITHAQA
VRAKATPLVLHYTPVTQQGLAPYFTEYIRQTIKSSSMLEGVNVYRDGLTIQTTLDSRMQQYAQQAIGEQAAALQAQFDRS
WRCPESLKIQFIKESARYKEMVNEEGVPANTALARLKADSAWLNNLLHQKTRVQMALIALDPSNGHVKAWVGGTNFGPDD
YRYQFDHVWQAKRQPGSAFKPFVYAAAIDQGIPANYRILDQPLALKSGNGGVWVARNAEGGSGGMTTLRDALAHSLNQVT
VRLAYEFLSPSEIISYARRMGITSPMQPNLSIALGTSEVSPLELARAYTTFANNGAWCDVLPVTKVLDKHGRVIAEHQPS
SHLGLDPATNYVMVTMLKDVIDRGTGIAARTRYGLDMEVAGKTGTTQSQRDAWFAGFTPKLVAVVWAGFDDERIHFTSME
YGQGARAALPAWANFMKKCYSDPSLKLEKTYFPMPDNVIAVPIAGSSTEPSDVLNRNVSVEYFTPKGYARYQAGDFNRAP
QPVNSGAVGNPDSTGAQQPPVPTAPTAPTAPASTDSTGSRARR

Sequences:

>Translated_763_residues
MSKKFVMKIAGLLLVVLLGAGAVFGLDLFKGLPSVQELENPKPELASLVYSEDGKLLHKFFLKNRTFVPLRSISRWAPAA
LIATEDVTFYQHWGVDLRRLAIALGENIIKGRTRWQGASTITQQLAKNLFLTQERTLSRKAKELVTAVQLERTYTKNEIL
ALYLNTVYFGSGAYGIESAAQTYFGKPASQLTIPESATLIATLKNPTAYDPSKDPSSSLARRNLIIGLMEKAGFITHAQA
VRAKATPLVLHYTPVTQQGLAPYFTEYIRQTIKSSSMLEGVNVYRDGLTIQTTLDSRMQQYAQQAIGEQAAALQAQFDRS
WRCPESLKIQFIKESARYKEMVNEEGVPANTALARLKADSAWLNNLLHQKTRVQMALIALDPSNGHVKAWVGGTNFGPDD
YRYQFDHVWQAKRQPGSAFKPFVYAAAIDQGIPANYRILDQPLALKSGNGGVWVARNAEGGSGGMTTLRDALAHSLNQVT
VRLAYEFLSPSEIISYARRMGITSPMQPNLSIALGTSEVSPLELARAYTTFANNGAWCDVLPVTKVLDKHGRVIAEHQPS
SHLGLDPATNYVMVTMLKDVIDRGTGIAARTRYGLDMEVAGKTGTTQSQRDAWFAGFTPKLVAVVWAGFDDERIHFTSME
YGQGARAALPAWANFMKKCYSDPSLKLEKTYFPMPDNVIAVPIAGSSTEPSDVLNRNVSVEYFTPKGYARYQAGDFNRAP
QPVNSGAVGNPDSTGAQQPPVPTAPTAPTAPASTDSTGSRARR
>Mature_762_residues
SKKFVMKIAGLLLVVLLGAGAVFGLDLFKGLPSVQELENPKPELASLVYSEDGKLLHKFFLKNRTFVPLRSISRWAPAAL
IATEDVTFYQHWGVDLRRLAIALGENIIKGRTRWQGASTITQQLAKNLFLTQERTLSRKAKELVTAVQLERTYTKNEILA
LYLNTVYFGSGAYGIESAAQTYFGKPASQLTIPESATLIATLKNPTAYDPSKDPSSSLARRNLIIGLMEKAGFITHAQAV
RAKATPLVLHYTPVTQQGLAPYFTEYIRQTIKSSSMLEGVNVYRDGLTIQTTLDSRMQQYAQQAIGEQAAALQAQFDRSW
RCPESLKIQFIKESARYKEMVNEEGVPANTALARLKADSAWLNNLLHQKTRVQMALIALDPSNGHVKAWVGGTNFGPDDY
RYQFDHVWQAKRQPGSAFKPFVYAAAIDQGIPANYRILDQPLALKSGNGGVWVARNAEGGSGGMTTLRDALAHSLNQVTV
RLAYEFLSPSEIISYARRMGITSPMQPNLSIALGTSEVSPLELARAYTTFANNGAWCDVLPVTKVLDKHGRVIAEHQPSS
HLGLDPATNYVMVTMLKDVIDRGTGIAARTRYGLDMEVAGKTGTTQSQRDAWFAGFTPKLVAVVWAGFDDERIHFTSMEY
GQGARAALPAWANFMKKCYSDPSLKLEKTYFPMPDNVIAVPIAGSSTEPSDVLNRNVSVEYFTPKGYARYQAGDFNRAPQ
PVNSGAVGNPDSTGAQQPPVPTAPTAPTAPASTDSTGSRARR

Specific function: Cell Wall Formation. Synthesis Of Cross-Linked Peptidoglycan From The Lipid Intermediates. The Enzyme Has A Penicillin-Insensitive Transglycosylase N-Terminal Domain (Formation Of Linear Glycan Strands) And A Penicillin-Sensitive Transpeptidase C-Terminal

COG id: COG5009

COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=609, Percent_Identity=28.735632183908, Blast_Score=218, Evalue=2e-57,
Organism=Escherichia coli, GI87082258, Length=305, Percent_Identity=39.0163934426229, Blast_Score=199, Evalue=4e-52,
Organism=Escherichia coli, GI1788867, Length=621, Percent_Identity=26.8921095008052, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI1789601, Length=233, Percent_Identity=35.6223175965665, Blast_Score=127, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.-

Molecular weight: Translated: 83629; Mature: 83498

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKFVMKIAGLLLVVLLGAGAVFGLDLFKGLPSVQELENPKPELASLVYSEDGKLLHKF
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHCCCCHHHHHHHHCCCCHHHHHH
FLKNRTFVPLRSISRWAPAALIATEDVTFYQHWGVDLRRLAIALGENIIKGRTRWQGAST
HHCCCCEEEHHHHHCCCCEEEEEECCCEEHHHCCCHHHHHHHHHCCHHHCCCCCCCCHHH
ITQQLAKNLFLTQERTLSRKAKELVTAVQLERTYTKNEILALYLNTVYFGSGAYGIESAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHEECCCCCCHHHHH
QTYFGKPASQLTIPESATLIATLKNPTAYDPSKDPSSSLARRNLIIGLMEKAGFITHAQA
HHHCCCCCCCEECCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHEEHHHHCCCHHHHHH
VRAKATPLVLHYTPVTQQGLAPYFTEYIRQTIKSSSMLEGVNVYRDGLTIQTTLDSRMQQ
HHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHH
YAQQAIGEQAAALQAQFDRSWRCPESLKIQFIKESARYKEMVNEEGVPANTALARLKADS
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
AWLNNLLHQKTRVQMALIALDPSNGHVKAWVGGTNFGPDDYRYQFDHVWQAKRQPGSAFK
HHHHHHHHHHHEEEEEEEEECCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCC
PFVYAAAIDQGIPANYRILDQPLALKSGNGGVWVARNAEGGSGGMTTLRDALAHSLNQVT
CHHEEEHHHCCCCCCCEEECCCEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHH
VRLAYEFLSPSEIISYARRMGITSPMQPNLSIALGTSEVSPLELARAYTTFANNGAWCDV
HHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCEEEH
LPVTKVLDKHGRVIAEHQPSSHLGLDPATNYVMVTMLKDVIDRGTGIAARTRYGLDMEVA
HHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCEEEC
GKTGTTQSQRDAWFAGFTPKLVAVVWAGFDDERIHFTSMEYGQGARAALPAWANFMKKCY
CCCCCCCCCCCCEECCCCHHHHHHEECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHC
SDPSLKLEKTYFPMPDNVIAVPIAGSSTEPSDVLNRNVSVEYFTPKGYARYQAGDFNRAP
CCCCCEEEEEECCCCCCEEEEEECCCCCCCHHHHCCCCEEEEECCCCCEEEECCCCCCCC
QPVNSGAVGNPDSTGAQQPPVPTAPTAPTAPASTDSTGSRARR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SKKFVMKIAGLLLVVLLGAGAVFGLDLFKGLPSVQELENPKPELASLVYSEDGKLLHKF
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHCCCCHHHHHHHHCCCCHHHHHH
FLKNRTFVPLRSISRWAPAALIATEDVTFYQHWGVDLRRLAIALGENIIKGRTRWQGAST
HHCCCCEEEHHHHHCCCCEEEEEECCCEEHHHCCCHHHHHHHHHCCHHHCCCCCCCCHHH
ITQQLAKNLFLTQERTLSRKAKELVTAVQLERTYTKNEILALYLNTVYFGSGAYGIESAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHEECCCCCCHHHHH
QTYFGKPASQLTIPESATLIATLKNPTAYDPSKDPSSSLARRNLIIGLMEKAGFITHAQA
HHHCCCCCCCEECCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHEEHHHHCCCHHHHHH
VRAKATPLVLHYTPVTQQGLAPYFTEYIRQTIKSSSMLEGVNVYRDGLTIQTTLDSRMQQ
HHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHH
YAQQAIGEQAAALQAQFDRSWRCPESLKIQFIKESARYKEMVNEEGVPANTALARLKADS
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
AWLNNLLHQKTRVQMALIALDPSNGHVKAWVGGTNFGPDDYRYQFDHVWQAKRQPGSAFK
HHHHHHHHHHHEEEEEEEEECCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCC
PFVYAAAIDQGIPANYRILDQPLALKSGNGGVWVARNAEGGSGGMTTLRDALAHSLNQVT
CHHEEEHHHCCCCCCCEEECCCEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHH
VRLAYEFLSPSEIISYARRMGITSPMQPNLSIALGTSEVSPLELARAYTTFANNGAWCDV
HHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCEEEH
LPVTKVLDKHGRVIAEHQPSSHLGLDPATNYVMVTMLKDVIDRGTGIAARTRYGLDMEVA
HHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCEEEC
GKTGTTQSQRDAWFAGFTPKLVAVVWAGFDDERIHFTSMEYGQGARAALPAWANFMKKCY
CCCCCCCCCCCCEECCCCHHHHHHEECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHC
SDPSLKLEKTYFPMPDNVIAVPIAGSSTEPSDVLNRNVSVEYFTPKGYARYQAGDFNRAP
CCCCCEEEEEECCCCCCEEEEEECCCCCCCHHHHCCCCEEEEECCCCCEEEECCCCCCCC
QPVNSGAVGNPDSTGAQQPPVPTAPTAPTAPASTDSTGSRARR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]