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The map label for this gene is fusA-2 [H]

Identifier: 21672985

GI number: 21672985

Start: 144731

End: 146809

Strand: Reverse

Name: fusA-2 [H]

Synonym: CT0144

Alternate gene names: 21672985

Gene position: 146809-144731 (Counterclockwise)

Preceding gene: 21672998

Following gene: 21672984

Centisome position: 6.81

GC content: 56.13

Gene sequence:

>2079_bases
ATGCAAGCTGTTCCAACGGATCAATTGAGGAACATTGTCGTTACCGGCCATTCCGGCACCGGAAAAACCATGCTGTGCGA
ATCACTCGCCCTCTGCATGGGTGTCATCAACCGGCTCGGCAGCATAGAGGATGGCACTACCCTGTCAGATTACGCTTCCG
ACGAGACAGAGAGAAAGCACAGCCTGAACACCAGTCTCATCCACGGCGTATGGAACGAGAAGAAGATCAATATTATCGAT
ACTCCAGGCCTGCTTGATTTTCACGGAGACGTCAAATCGGCCATGCGCGTTGCCGATACGGTGCTGATCACGGTCAACGC
GGCCACGGGCGTGGAGGTCGGCACAGACACGGTGTGGGAGTACACCAAGGAGTACTACAAGCCGACCATGTTCGTGCTTA
CCAAGCTCGATGCCGATCGCGCGGATTACAACGCAACCATCGAAGCCCTGCGCGACCACTTCGGCCATCTGGTTACGCCA
ATCCAGTTCCCAGCCGAGGAGGGATTCGGCCACCATATCCTGATCGACGTCCTGCTGATGAAGCAGATCGAGTTCAGCCC
CGACAAACCTGGCAGCATGGTGATTTCAGAAATTCATGACCTGTACCGGAAAAAGGCGGAGGTGCTTCACCAGCAACTGG
TCGAAGCGGTCGCCGAAACCGACGAGGAGCTGATGAACCACTTCTTCGAAGAGGGTACGCTCACCGAAGACGAGCTTCGG
GCCGGCATCAAATCGGCTCTCGTCACCCGCACCTTCTTCCCTGTTTTCTGCACCTCACCGCTCCATCTCATCGGCTCCGA
ACGGCTTCTGAACGCTATCGTCAACCTCTGTCCGTCGCCCATCGAGCGCGGGCCGGAACATGCGTTCTGCTCGGTGATGA
ACGACGAAAAGCTGCTGCCTCCCGATCCCGACGGATCGACCATTGCCTTTATTTTCAAGACCATGTCGGAACCGAGAGTG
GGCGAAATCTCCTACATTCGCGTCTACTCGGGCCACATCGAGAGTGGGCACGAACTGATCGACGTCCAGACCGGTCAGCT
CGAAAAGCTCGGACAGGTCTACACAATGCTGGGGCAGAAGAAGATTCCGGTTGACAAGCTGCTGGCAGGCGATATCGGCA
TGGTGGTCAAGCTGAAAAACTCGCACACCAACGACACGCTCGCCGACAAAGGGGTGAATTGCCGGATCAGCCCGATCATC
TTTCCGGAGCCGGTACTCTCCTCGGCCATCGTGCCGGTCACGCAGGGCGACGAAGAGAAGATATCCGCCGGCTTGCACCA
TCTTCACGAAGAAGATCCGAGCTTCGCCATCGAGCATGATGTCGAGTTCAACCAGACCATTCTCAAAACACTTGGGGAGA
CGCATCTCGACATCATCATCAGTCGCCTGCGAAACAAGTTCAATATCCAGGTAGAGGTAGCGCCTGTAAGGATTCCCTAT
CGTGAAACCATCAGGGTAAGTGCGTCAGCGCAGGGAAAATTCAAGAAACAATCAGGCGGCCGCGGCCAGTACGGCGATGT
CTGGATTCGCATCGAGCCTCTGGAGCGGGGCTCGGGCTTCGAGTTCGCCAGTGAAGTGGTCGGCGGCGTGGTCCCAACGC
GCTACATCCCGGCGGTCGAAAAAGGATTGCGTGAATCGATCGCTGAGGGAAGTCTTGCCGGCTACCCCGTGGTCGATCTG
AAAGCGGTGGTTTATGACGGATCGCATCATCCGGTGGACAGCTCGGAATATGCATTCAAGATCGCCGCCAGCATGGCATT
CAAGGCCGCTGTCGAAAAGGCAAAACCGCTGATTCTCGAACCGATCTACTCGCTGACCGTACAAACCCCCGACCAGTTCA
CGGGCGAAATCGTGGGAGACATATCGAGCAAGCGGGGCAGGATTCTCGGCATGGACACTGAATCCCGATTCCAGGTCATC
AAGGCGCTCATTCCACAGGCTTCACTTTCGACATTCCATCACGCACTGACAAGGCTGACCCAGAGCCGCGCTCGATACAA
CTATACCTTCAGCCATTACGAAGAAGCCCCGGCCGAAATAGCAAACCAGCTCATCGCGGAGAAAACCGCAAAACAGTAA

Upstream 100 bases:

>100_bases
TTGGCGCCCGAGGCTGACTTCCCGTGTGATGCTCTTTCAGGCGAGAAACCGGCAAAAGCAGGCGGCTCCGTATAAATCAA
TCCAAGAAAGGAGTACTGAC

Downstream 100 bases:

>100_bases
AAAAAAAGGGGCGTGGCAGCATCAATCATCTCATCATGCTGCCATGTCCCCCTCGTCTCCGCTATTGTAGAACCCCCGTG
CCCAAAACAGTAAAGCCCGT

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: EF-G [H]

Number of amino acids: Translated: 692; Mature: 692

Protein sequence:

>692_residues
MQAVPTDQLRNIVVTGHSGTGKTMLCESLALCMGVINRLGSIEDGTTLSDYASDETERKHSLNTSLIHGVWNEKKINIID
TPGLLDFHGDVKSAMRVADTVLITVNAATGVEVGTDTVWEYTKEYYKPTMFVLTKLDADRADYNATIEALRDHFGHLVTP
IQFPAEEGFGHHILIDVLLMKQIEFSPDKPGSMVISEIHDLYRKKAEVLHQQLVEAVAETDEELMNHFFEEGTLTEDELR
AGIKSALVTRTFFPVFCTSPLHLIGSERLLNAIVNLCPSPIERGPEHAFCSVMNDEKLLPPDPDGSTIAFIFKTMSEPRV
GEISYIRVYSGHIESGHELIDVQTGQLEKLGQVYTMLGQKKIPVDKLLAGDIGMVVKLKNSHTNDTLADKGVNCRISPII
FPEPVLSSAIVPVTQGDEEKISAGLHHLHEEDPSFAIEHDVEFNQTILKTLGETHLDIIISRLRNKFNIQVEVAPVRIPY
RETIRVSASAQGKFKKQSGGRGQYGDVWIRIEPLERGSGFEFASEVVGGVVPTRYIPAVEKGLRESIAEGSLAGYPVVDL
KAVVYDGSHHPVDSSEYAFKIAASMAFKAAVEKAKPLILEPIYSLTVQTPDQFTGEIVGDISSKRGRILGMDTESRFQVI
KALIPQASLSTFHHALTRLTQSRARYNYTFSHYEEAPAEIANQLIAEKTAKQ

Sequences:

>Translated_692_residues
MQAVPTDQLRNIVVTGHSGTGKTMLCESLALCMGVINRLGSIEDGTTLSDYASDETERKHSLNTSLIHGVWNEKKINIID
TPGLLDFHGDVKSAMRVADTVLITVNAATGVEVGTDTVWEYTKEYYKPTMFVLTKLDADRADYNATIEALRDHFGHLVTP
IQFPAEEGFGHHILIDVLLMKQIEFSPDKPGSMVISEIHDLYRKKAEVLHQQLVEAVAETDEELMNHFFEEGTLTEDELR
AGIKSALVTRTFFPVFCTSPLHLIGSERLLNAIVNLCPSPIERGPEHAFCSVMNDEKLLPPDPDGSTIAFIFKTMSEPRV
GEISYIRVYSGHIESGHELIDVQTGQLEKLGQVYTMLGQKKIPVDKLLAGDIGMVVKLKNSHTNDTLADKGVNCRISPII
FPEPVLSSAIVPVTQGDEEKISAGLHHLHEEDPSFAIEHDVEFNQTILKTLGETHLDIIISRLRNKFNIQVEVAPVRIPY
RETIRVSASAQGKFKKQSGGRGQYGDVWIRIEPLERGSGFEFASEVVGGVVPTRYIPAVEKGLRESIAEGSLAGYPVVDL
KAVVYDGSHHPVDSSEYAFKIAASMAFKAAVEKAKPLILEPIYSLTVQTPDQFTGEIVGDISSKRGRILGMDTESRFQVI
KALIPQASLSTFHHALTRLTQSRARYNYTFSHYEEAPAEIANQLIAEKTAKQ
>Mature_692_residues
MQAVPTDQLRNIVVTGHSGTGKTMLCESLALCMGVINRLGSIEDGTTLSDYASDETERKHSLNTSLIHGVWNEKKINIID
TPGLLDFHGDVKSAMRVADTVLITVNAATGVEVGTDTVWEYTKEYYKPTMFVLTKLDADRADYNATIEALRDHFGHLVTP
IQFPAEEGFGHHILIDVLLMKQIEFSPDKPGSMVISEIHDLYRKKAEVLHQQLVEAVAETDEELMNHFFEEGTLTEDELR
AGIKSALVTRTFFPVFCTSPLHLIGSERLLNAIVNLCPSPIERGPEHAFCSVMNDEKLLPPDPDGSTIAFIFKTMSEPRV
GEISYIRVYSGHIESGHELIDVQTGQLEKLGQVYTMLGQKKIPVDKLLAGDIGMVVKLKNSHTNDTLADKGVNCRISPII
FPEPVLSSAIVPVTQGDEEKISAGLHHLHEEDPSFAIEHDVEFNQTILKTLGETHLDIIISRLRNKFNIQVEVAPVRIPY
RETIRVSASAQGKFKKQSGGRGQYGDVWIRIEPLERGSGFEFASEVVGGVVPTRYIPAVEKGLRESIAEGSLAGYPVVDL
KAVVYDGSHHPVDSSEYAFKIAASMAFKAAVEKAKPLILEPIYSLTVQTPDQFTGEIVGDISSKRGRILGMDTESRFQVI
KALIPQASLSTFHHALTRLTQSRARYNYTFSHYEEAPAEIANQLIAEKTAKQ

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=684, Percent_Identity=30.4093567251462, Blast_Score=325, Evalue=1e-88,
Organism=Homo sapiens, GI19923640, Length=731, Percent_Identity=24.8974008207934, Blast_Score=246, Evalue=6e-65,
Organism=Homo sapiens, GI25306287, Length=716, Percent_Identity=23.7430167597765, Blast_Score=193, Evalue=5e-49,
Organism=Homo sapiens, GI25306283, Length=399, Percent_Identity=26.8170426065163, Blast_Score=142, Evalue=1e-33,
Organism=Escherichia coli, GI1789738, Length=704, Percent_Identity=36.2215909090909, Blast_Score=454, Evalue=1e-129,
Organism=Escherichia coli, GI1790835, Length=491, Percent_Identity=23.6252545824847, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI48994988, Length=151, Percent_Identity=29.8013245033113, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17533571, Length=697, Percent_Identity=27.5466284074605, Blast_Score=287, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI17556745, Length=712, Percent_Identity=23.5955056179775, Blast_Score=169, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6323098, Length=700, Percent_Identity=29.2857142857143, Blast_Score=310, Evalue=4e-85,
Organism=Saccharomyces cerevisiae, GI6322359, Length=781, Percent_Identity=26.1203585147247, Blast_Score=249, Evalue=8e-67,
Organism=Drosophila melanogaster, GI24582462, Length=705, Percent_Identity=28.3687943262411, Blast_Score=313, Evalue=2e-85,
Organism=Drosophila melanogaster, GI221458488, Length=704, Percent_Identity=24.5738636363636, Blast_Score=195, Evalue=8e-50,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 76539; Mature: 76539

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAVPTDQLRNIVVTGHSGTGKTMLCESLALCMGVINRLGSIEDGTTLSDYASDETERKH
CCCCCCHHHCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCHHHHHH
SLNTSLIHGVWNEKKINIIDTPGLLDFHGDVKSAMRVADTVLITVNAATGVEVGTDTVWE
HCCHHHHHCCCCCCEEEEEECCCCEECCCHHHHHHHHHCEEEEEEECCCCCCCCCHHHHH
YTKEYYKPTMFVLTKLDADRADYNATIEALRDHFGHLVTPIQFPAEEGFGHHILIDVLLM
HHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
KQIEFSPDKPGSMVISEIHDLYRKKAEVLHQQLVEAVAETDEELMNHFFEEGTLTEDELR
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
AGIKSALVTRTFFPVFCTSPLHLIGSERLLNAIVNLCPSPIERGPEHAFCSVMNDEKLLP
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHEECCCCCCCC
PDPDGSTIAFIFKTMSEPRVGEISYIRVYSGHIESGHELIDVQTGQLEKLGQVYTMLGQK
CCCCCCEEEEEEECCCCCCCCCEEEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHCCC
KIPVDKLLAGDIGMVVKLKNSHTNDTLADKGVNCRISPIIFPEPVLSSAIVPVTQGDEEK
CCCHHHHHCCCCEEEEEEECCCCCCHHHCCCCCEEECCEECCCHHHHCCEEECCCCCHHH
ISAGLHHLHEEDPSFAIEHDVEFNQTILKTLGETHLDIIISRLRNKFNIQVEVAPVRIPY
HHHHHHHHHCCCCCEEEECCCCHHHHHHHHHCHHHHHHHHHHHCCCEEEEEEEEEEECCH
RETIRVSASAQGKFKKQSGGRGQYGDVWIRIEPLERGSGFEFASEVVGGVVPTRYIPAVE
HHHEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHCCCCCHHHHHHHH
KGLRESIAEGSLAGYPVVDLKAVVYDGSHHPVDSSEYAFKIAASMAFKAAVEKAKPLILE
HHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEE
PIYSLTVQTPDQFTGEIVGDISSKRGRILGMDTESRFQVIKALIPQASLSTFHHALTRLT
CHHHEEECCCHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHH
QSRARYNYTFSHYEEAPAEIANQLIAEKTAKQ
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQAVPTDQLRNIVVTGHSGTGKTMLCESLALCMGVINRLGSIEDGTTLSDYASDETERKH
CCCCCCHHHCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCHHHHHH
SLNTSLIHGVWNEKKINIIDTPGLLDFHGDVKSAMRVADTVLITVNAATGVEVGTDTVWE
HCCHHHHHCCCCCCEEEEEECCCCEECCCHHHHHHHHHCEEEEEEECCCCCCCCCHHHHH
YTKEYYKPTMFVLTKLDADRADYNATIEALRDHFGHLVTPIQFPAEEGFGHHILIDVLLM
HHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
KQIEFSPDKPGSMVISEIHDLYRKKAEVLHQQLVEAVAETDEELMNHFFEEGTLTEDELR
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
AGIKSALVTRTFFPVFCTSPLHLIGSERLLNAIVNLCPSPIERGPEHAFCSVMNDEKLLP
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHEECCCCCCCC
PDPDGSTIAFIFKTMSEPRVGEISYIRVYSGHIESGHELIDVQTGQLEKLGQVYTMLGQK
CCCCCCEEEEEEECCCCCCCCCEEEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHCCC
KIPVDKLLAGDIGMVVKLKNSHTNDTLADKGVNCRISPIIFPEPVLSSAIVPVTQGDEEK
CCCHHHHHCCCCEEEEEEECCCCCCHHHCCCCCEEECCEECCCHHHHCCEEECCCCCHHH
ISAGLHHLHEEDPSFAIEHDVEFNQTILKTLGETHLDIIISRLRNKFNIQVEVAPVRIPY
HHHHHHHHHCCCCCEEEECCCCHHHHHHHHHCHHHHHHHHHHHCCCEEEEEEEEEEECCH
RETIRVSASAQGKFKKQSGGRGQYGDVWIRIEPLERGSGFEFASEVVGGVVPTRYIPAVE
HHHEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHCCCCCHHHHHHHH
KGLRESIAEGSLAGYPVVDLKAVVYDGSHHPVDSSEYAFKIAASMAFKAAVEKAKPLILE
HHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEE
PIYSLTVQTPDQFTGEIVGDISSKRGRILGMDTESRFQVIKALIPQASLSTFHHALTRLT
CHHHEEECCCHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHH
QSRARYNYTFSHYEEAPAEIANQLIAEKTAKQ
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376 [H]