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The map label for this gene is emrA [H]

Identifier: 21672946

GI number: 21672946

Start: 99009

End: 100001

Strand: Reverse

Name: emrA [H]

Synonym: CT0105

Alternate gene names: 21672946

Gene position: 100001-99009 (Counterclockwise)

Preceding gene: 21672947

Following gene: 21672945

Centisome position: 4.64

GC content: 59.62

Gene sequence:

>993_bases
ATGGCAGAAACGCAGCAATCCAACATCGAAGCACCGGACAACGAAAAAGGCAAGCCAAAACCGGAACGCTCGATGGGCCG
CCTGGTCATCTTCGGCATTTTGCTGGCCATCGGCCTTGTCTGGGGCGGCATGAAGCTCATCCGCTCGCTCAGTTACGAAG
AGACCGATGACGCGCAGATCGCCGGCAACATCTATCCGGTCATTCCGCGCGTGCCGGGCAAGGTGGTCGAAGTGCTTGCC
AACGACAACCAGATGGTGAAAAAAGGCGACGTGCTCATCCGGCTCGATCCTTCGGACTACCAGATCAAGCGCGATATGGC
CGAAGCGCAGCTCCTGAAAGCGCGGGCGGCAGTATCGGGAGCGAAGGCCGACATCATAGCGGCGGCGGCGGCGCAAATCA
AGCTTGCCGCCGACCTTCGCCGCAGCCAGAACCTCCAGAAACAGGATGTGATCTCCCGAGCGGAGCTCGATGCCGCCACG
GCTGGCGCGACCGCCGCGTCTGCACAGCACGCCGCCGCCGGAGACAACTACAAGGCCGCACTCGCCCAGGCAAAACTTGC
CGAGGCCGAGCTGAAAAACGCCGAACTCCAGCTGTCATGGACAACCATTACCGCTCCGGCTGACGGCAAGGTTTCGAAAA
AGAACGTCCAGCCCGGCCAGTATGTTACTCCCGGCCAACAGCTCATCGCCATTGTCGGCAGCGGCGACTTGTGGGTCGTG
GCCAACTTCAAGGAAACGCAACTCGAACACATGCGCCCCGGCCAAAAGGTAATCATCAAAGTCGATGCCTTCCCAGGCAA
GGAGCTGAAAGGCCATATCGATTCAATCTCCGCAGGAACAGGCGCCGAGTTCGCCCTGCTTCCCCCCGATAACGCCAGCG
GCAACTTCGTAAAAGTGACCCAGCGTGTGCCGGTCAAGATCGTCTTCGACGAAAAAACCGACCTGCCGCTTGCCGCTGGA
ATGAATGTGATCGCAGAAGTGAAGGTGAAGTGA

Upstream 100 bases:

>100_bases
GCTGGTGCTCCGCTCGCCGGAAGGCGTCAATGAAACCGGAACGGTTCAGCGGAAACAAAAAGAAGCAATCCTGTAAACAC
CAACGACAGATTTACTATCC

Downstream 100 bases:

>100_bases
TGTCAAGTACCGCAACAACCATACCGGGCGCTCAGGCGCTGGAACATCATTACGAAACCGGGGCCAGAAAATGGATCATC
ACAGCAACGGTGATCATCGC

Product: multidrug resistance protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MAETQQSNIEAPDNEKGKPKPERSMGRLVIFGILLAIGLVWGGMKLIRSLSYEETDDAQIAGNIYPVIPRVPGKVVEVLA
NDNQMVKKGDVLIRLDPSDYQIKRDMAEAQLLKARAAVSGAKADIIAAAAAQIKLAADLRRSQNLQKQDVISRAELDAAT
AGATAASAQHAAAGDNYKAALAQAKLAEAELKNAELQLSWTTITAPADGKVSKKNVQPGQYVTPGQQLIAIVGSGDLWVV
ANFKETQLEHMRPGQKVIIKVDAFPGKELKGHIDSISAGTGAEFALLPPDNASGNFVKVTQRVPVKIVFDEKTDLPLAAG
MNVIAEVKVK

Sequences:

>Translated_330_residues
MAETQQSNIEAPDNEKGKPKPERSMGRLVIFGILLAIGLVWGGMKLIRSLSYEETDDAQIAGNIYPVIPRVPGKVVEVLA
NDNQMVKKGDVLIRLDPSDYQIKRDMAEAQLLKARAAVSGAKADIIAAAAAQIKLAADLRRSQNLQKQDVISRAELDAAT
AGATAASAQHAAAGDNYKAALAQAKLAEAELKNAELQLSWTTITAPADGKVSKKNVQPGQYVTPGQQLIAIVGSGDLWVV
ANFKETQLEHMRPGQKVIIKVDAFPGKELKGHIDSISAGTGAEFALLPPDNASGNFVKVTQRVPVKIVFDEKTDLPLAAG
MNVIAEVKVK
>Mature_329_residues
AETQQSNIEAPDNEKGKPKPERSMGRLVIFGILLAIGLVWGGMKLIRSLSYEETDDAQIAGNIYPVIPRVPGKVVEVLAN
DNQMVKKGDVLIRLDPSDYQIKRDMAEAQLLKARAAVSGAKADIIAAAAAQIKLAADLRRSQNLQKQDVISRAELDAATA
GATAASAQHAAAGDNYKAALAQAKLAEAELKNAELQLSWTTITAPADGKVSKKNVQPGQYVTPGQQLIAIVGSGDLWVVA
NFKETQLEHMRPGQKVIIKVDAFPGKELKGHIDSISAGTGAEFALLPPDNASGNFVKVTQRVPVKIVFDEKTDLPLAAGM
NVIAEVKVK

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=355, Percent_Identity=36.056338028169, Blast_Score=176, Evalue=1e-45,
Organism=Escherichia coli, GI1788711, Length=335, Percent_Identity=33.134328358209, Blast_Score=147, Evalue=9e-37,
Organism=Escherichia coli, GI1790519, Length=290, Percent_Identity=31.3793103448276, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI87081951, Length=275, Percent_Identity=31.2727272727273, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI1789637, Length=274, Percent_Identity=31.021897810219, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1790024, Length=267, Percent_Identity=27.7153558052434, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790012, Length=273, Percent_Identity=30.03663003663, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI87082036, Length=165, Percent_Identity=32.7272727272727, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787013, Length=247, Percent_Identity=27.1255060728745, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 35185; Mature: 35054

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAETQQSNIEAPDNEKGKPKPERSMGRLVIFGILLAIGLVWGGMKLIRSLSYEETDDAQI
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
AGNIYPVIPRVPGKVVEVLANDNQMVKKGDVLIRLDPSDYQIKRDMAEAQLLKARAAVSG
CCCEEEECCCCCHHHHHHHCCCCCEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCC
AKADIIAAAAAQIKLAADLRRSQNLQKQDVISRAELDAATAGATAASAQHAAAGDNYKAA
CCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHCCCCHHCCHHHCCCCCHHHH
LAQAKLAEAELKNAELQLSWTTITAPADGKVSKKNVQPGQYVTPGQQLIAIVGSGDLWVV
HHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCCCCCCEECCHHHEEEEEECCCEEEE
ANFKETQLEHMRPGQKVIIKVDAFPGKELKGHIDSISAGTGAEFALLPPDNASGNFVKVT
ECCHHHHHHHCCCCCEEEEEEECCCCHHHHCCCCCCCCCCCCEEEEECCCCCCCCEEEEE
QRVPVKIVFDEKTDLPLAAGMNVIAEVKVK
ECCCEEEEECCCCCCCHHHCCCEEEEEEEC
>Mature Secondary Structure 
AETQQSNIEAPDNEKGKPKPERSMGRLVIFGILLAIGLVWGGMKLIRSLSYEETDDAQI
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
AGNIYPVIPRVPGKVVEVLANDNQMVKKGDVLIRLDPSDYQIKRDMAEAQLLKARAAVSG
CCCEEEECCCCCHHHHHHHCCCCCEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCC
AKADIIAAAAAQIKLAADLRRSQNLQKQDVISRAELDAATAGATAASAQHAAAGDNYKAA
CCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHCCCCHHCCHHHCCCCCHHHH
LAQAKLAEAELKNAELQLSWTTITAPADGKVSKKNVQPGQYVTPGQQLIAIVGSGDLWVV
HHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCCCCCCEECCHHHEEEEEECCCEEEE
ANFKETQLEHMRPGQKVIIKVDAFPGKELKGHIDSISAGTGAEFALLPPDNASGNFVKVT
ECCHHHHHHHCCCCCEEEEEEECCCCHHHHCCCCCCCCCCCCEEEEECCCCCCCCEEEEE
QRVPVKIVFDEKTDLPLAAGMNVIAEVKVK
ECCCEEEEECCCCCCCHHHCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1409590; 9205837; 9278503; 8203018 [H]