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The map label for this gene is emrB [H]

Identifier: 21672945

GI number: 21672945

Start: 97408

End: 99009

Strand: Reverse

Name: emrB [H]

Synonym: CT0104

Alternate gene names: 21672945

Gene position: 99009-97408 (Counterclockwise)

Preceding gene: 21672946

Following gene: 21672941

Centisome position: 4.59

GC content: 60.11

Gene sequence:

>1602_bases
ATGTCAAGTACCGCAACAACCATACCGGGCGCTCAGGCGCTGGAACATCATTACGAAACCGGGGCCAGAAAATGGATCAT
CACAGCAACGGTGATCATCGCGGCGATGCTCGAACTGATCGACACCACCATCGTCAACGTGGCCCTCAACCACATCAGCG
GCAACCTCGGCGCGAGCATCGAGGATGTTTCCTACGTGGTCACGAGCTACGCCATCGCCAACGTGATTGTCATTCCACTG
TCGGGATTTCTCGGCAACCTGTTCGGGCGCCGCAACTACTTCGTGGCCTCGATCGTGCTCTTCACGGGCGCCTCGTTTTT
GTGCGGCATCTCGACAAACATCTGGATGCTGGTCTTCTTCCGCTTCATCCAGGGTATCGGCGGAGGAGGACTGCTGCCCA
CCTCGCAGGCGATCCTGTACGAAACCTTCAGGCCGGAGGAGCGCGGCGCGGCAACGGGCATCTTTTCGATGGGGCTGGTG
CTCGGCCCCACCATCGGCCCGCTGCTTGGCGGGTATCTGGTCGATTACTTCGCATGGGAGTGGTGCTTTTTCGTCAACAT
CCCGATCGGCATCGCGGCGGCCTGGGCGTCGCTCACCTTCGTGAAAGAGCCGAAGGTGAAGCCTGTGGTCGAAAAAATCG
ACTGGGCGGGCATCGGCCTGCTCTCCGTCGGCATCGGCTCGCTCCAGTTCGTGCTTGAGCGGGGCGAACAGAAGGACTGG
TTCGAGACCGATTATATCGTCTGGTTCACCATCATCGCCGTGGTTTCGCTCATCGCGTTTGTCTGGCTGGAGCTGCACAC
CGACCATCCGGCGGTTGACCTCTCCGTGCTGGCGCGAAGCAAAAACCTCGCCATCGGCGCGGTACTGACCTTCATCGTCG
GCTTCGGCCTGTACGGTTCACTCTTCATCTTTCCGGTTTTCGTGCAGCGACTCCTCGGCTTCACGGCGCTCCTGACCGGT
CTGGTGCTCTTTCCGAGCGCCATGCTCACCGGCATCATTTCGATGCCGCTCGGTATCGCGTTGCAAAAGGGTGCGTCGCC
GAAGCTGCTCATGACGGTCGGCATGGTCGCTTTCTTCTGGTTCTGCTGGGAGCTTGGCAACCAGACGCTCATGTCGGGCG
CGGAGAACTTTTTTCTCGTGCTGCTCATTCGCGGCTTCGCCCTCGGCTTCATCTTCATTCCGGTCACCCTGCTCGCCGTC
ACGGGACTGCACGGCAAGGATATCGGGCAGGCGACCGGCCTGAACAATATGGTGCGTCAGCTTGGCGGTTCGTTCGGCAT
CGCCATCACCAACACCTACGTCACCCAGCGCGTGGCCGCGCACCGCATCGACATTCTCAGCCACCTCTCGCCCTACGACC
CCGCCGCCGTTCAGCGGTTGCAGGACATGAAGCAGGCGCTCGGTCAGTACATGAGTTCGCCGGTCGAAGCAGGACAGGCA
GCAATGGCTGCGCTCGAAGGCATCGTCGTCCGCCAGAGCTACCACCTGGCATACATGGACGCCTTCAAGATGATCGCCAT
CCTCTTCGCTGTCTGCCTGCCACTGCTGCTCTTTATCAGGGTGGACAAAAAAGAGACGGTGGATATGTCATCGGTGCACT
GA

Upstream 100 bases:

>100_bases
AAGTGACCCAGCGTGTGCCGGTCAAGATCGTCTTCGACGAAAAAACCGACCTGCCGCTTGCCGCTGGAATGAATGTGATC
GCAGAAGTGAAGGTGAAGTG

Downstream 100 bases:

>100_bases
AAAATATCGTGATATGACCGTGTGCCTGACATGCCGCACAACGGTCCAGCAAAGCCGCAGAAGATCACTGCGGCTTTGCT
GTTTCAGGCTGATCCGGCTC

Product: multidrug resistance protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 533; Mature: 532

Protein sequence:

>533_residues
MSSTATTIPGAQALEHHYETGARKWIITATVIIAAMLELIDTTIVNVALNHISGNLGASIEDVSYVVTSYAIANVIVIPL
SGFLGNLFGRRNYFVASIVLFTGASFLCGISTNIWMLVFFRFIQGIGGGGLLPTSQAILYETFRPEERGAATGIFSMGLV
LGPTIGPLLGGYLVDYFAWEWCFFVNIPIGIAAAWASLTFVKEPKVKPVVEKIDWAGIGLLSVGIGSLQFVLERGEQKDW
FETDYIVWFTIIAVVSLIAFVWLELHTDHPAVDLSVLARSKNLAIGAVLTFIVGFGLYGSLFIFPVFVQRLLGFTALLTG
LVLFPSAMLTGIISMPLGIALQKGASPKLLMTVGMVAFFWFCWELGNQTLMSGAENFFLVLLIRGFALGFIFIPVTLLAV
TGLHGKDIGQATGLNNMVRQLGGSFGIAITNTYVTQRVAAHRIDILSHLSPYDPAAVQRLQDMKQALGQYMSSPVEAGQA
AMAALEGIVVRQSYHLAYMDAFKMIAILFAVCLPLLLFIRVDKKETVDMSSVH

Sequences:

>Translated_533_residues
MSSTATTIPGAQALEHHYETGARKWIITATVIIAAMLELIDTTIVNVALNHISGNLGASIEDVSYVVTSYAIANVIVIPL
SGFLGNLFGRRNYFVASIVLFTGASFLCGISTNIWMLVFFRFIQGIGGGGLLPTSQAILYETFRPEERGAATGIFSMGLV
LGPTIGPLLGGYLVDYFAWEWCFFVNIPIGIAAAWASLTFVKEPKVKPVVEKIDWAGIGLLSVGIGSLQFVLERGEQKDW
FETDYIVWFTIIAVVSLIAFVWLELHTDHPAVDLSVLARSKNLAIGAVLTFIVGFGLYGSLFIFPVFVQRLLGFTALLTG
LVLFPSAMLTGIISMPLGIALQKGASPKLLMTVGMVAFFWFCWELGNQTLMSGAENFFLVLLIRGFALGFIFIPVTLLAV
TGLHGKDIGQATGLNNMVRQLGGSFGIAITNTYVTQRVAAHRIDILSHLSPYDPAAVQRLQDMKQALGQYMSSPVEAGQA
AMAALEGIVVRQSYHLAYMDAFKMIAILFAVCLPLLLFIRVDKKETVDMSSVH
>Mature_532_residues
SSTATTIPGAQALEHHYETGARKWIITATVIIAAMLELIDTTIVNVALNHISGNLGASIEDVSYVVTSYAIANVIVIPLS
GFLGNLFGRRNYFVASIVLFTGASFLCGISTNIWMLVFFRFIQGIGGGGLLPTSQAILYETFRPEERGAATGIFSMGLVL
GPTIGPLLGGYLVDYFAWEWCFFVNIPIGIAAAWASLTFVKEPKVKPVVEKIDWAGIGLLSVGIGSLQFVLERGEQKDWF
ETDYIVWFTIIAVVSLIAFVWLELHTDHPAVDLSVLARSKNLAIGAVLTFIVGFGLYGSLFIFPVFVQRLLGFTALLTGL
VLFPSAMLTGIISMPLGIALQKGASPKLLMTVGMVAFFWFCWELGNQTLMSGAENFFLVLLIRGFALGFIFIPVTLLAVT
GLHGKDIGQATGLNNMVRQLGGSFGIAITNTYVTQRVAAHRIDILSHLSPYDPAAVQRLQDMKQALGQYMSSPVEAGQAA
MAALEGIVVRQSYHLAYMDAFKMIAILFAVCLPLLLFIRVDKKETVDMSSVH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=505, Percent_Identity=33.8613861386139, Blast_Score=281, Evalue=8e-77,
Organism=Escherichia coli, GI1788710, Length=505, Percent_Identity=31.4851485148515, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI1790195, Length=305, Percent_Identity=30.1639344262295, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1788392, Length=291, Percent_Identity=32.3024054982818, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI87081983, Length=411, Percent_Identity=25.3041362530414, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI87082231, Length=238, Percent_Identity=22.2689075630252, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI87082320, Length=198, Percent_Identity=25.2525252525253, Blast_Score=63, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6322958, Length=513, Percent_Identity=21.6374269005848, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6319770, Length=386, Percent_Identity=26.6839378238342, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323735, Length=319, Percent_Identity=24.4514106583072, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6325455, Length=237, Percent_Identity=24.4725738396624, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=27.3333333333333, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324954, Length=331, Percent_Identity=24.4712990936556, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 57874; Mature: 57743

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SSTATTIPGAQALEHHYETGARKWIITATVIIAAMLELIDTTIVNVALNHISGNLGASI
CCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]