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The map label for this gene is clpB-2

Identifier: 21672930

GI number: 21672930

Start: 83816

End: 85132

Strand: Direct

Name: clpB-2

Synonym: CT0089

Alternate gene names: 21672930

Gene position: 83816-85132 (Clockwise)

Preceding gene: 21672927

Following gene: 21672931

Centisome position: 3.89

GC content: 60.14

Gene sequence:

>1317_bases
ATGAATACCGCAGATACCAGACAAAGGCTGCTCGACATCGAAAAGCAGATCGCGAGCCTCAGGGAGGAGCAGGCGACCGT
CAAGGCGCAGTGGGAGGCAGAGAAGGAGCTTATCCACACCTCCCGCCGTCTCAAGGAAGAGCTTGAAGACCTGCGTGTAC
AGGCTGAAAATTATGAACGCAGCGGTGATTACGGCAAGGTCGCTGAGATTCGCTACGGCAAGATCGCCGAGATCGAAAAG
GCGCTGGAGGAGAACAACCGCAAGATCGAAGCGCGACAGGCCTCCGGCGACCTCATTATGAAGGAGGAGATCGACGCGGG
CGACATCGCCGACATCGTTTCGCGATGGACGGGCATTCCGGTCAGCAAGATGCTCCAGTCCGAGCGGCAGAAGCTGCTCG
GCATCGAGTCCGAGCTGCACCGCAGGGTGGTTGGCCAGGATGAAGCTGTCCGCGCGGTCAGCGACGCGGTCAAGCGTTCG
CGCGCGGGCATGGGCGACGAGAAGCGACCCATCGGCTCGTTCATTTTCCTCGGCCCGACCGGCGTCGGCAAGACCGAGCT
GGCCCGCACGCTGGCCGAATACCTGTTCGACGACGAGGACGCGCTAATCCGCATCGACATGAGCGAGTACATGGAGGCGC
ACACCGTGAGCCGCCTTGTCGGAGCACCTCCCGGCTACGTCGGCTACGAAGAGGGCGGTCAGCTTACCGAAGCGGTACGG
CGCAAGCCCTTCTCGGTGGTGTTGCTCGACGAAATCGAAAAGGCGCACCCGGATGTGTTCAACATCCTGCTCCAGATTCT
CGACGACGGGCGGCTGACCGACAGCAAAGGGCGCACGGTGAACTTCAAGAACACCATCATCATCATGACCTCGAACATCG
GCGCGCAGCTCATTCAGAGCGAAATGGAGCATCTCGAAGGGCGCGATGCTGATGCGGCGCTGGCCGGGTTGAAGGAGAAG
CTCTTCCAGTTGCTCAAGCAGCAGGTGCGGCCTGAGTTCTTGAACCGCATCGACGAGGTAATTCTCTTCACGCCGCTGAC
ACGTGAAAACCTGCGGGAGATCGTCACCATTCAGTTCAACCGGATCAAGGAGACCGCCAGGCGCCAGCGCATCACCCTCG
AAATCTCGGACGAGGCGCTCATGTGGCTTGCCAAAACCGGCTTTGACCCGGCATTCGGCGCGCGCCCGCTCAAGCGCGTC
ATGCAACGGCAGATTACTAACCGCCTTTCGGAGATGATCCTCGCCGGTCAGGTTGGCGAAGACGACACCGTCGAGATCGG
GCTGGAGAACGATGCCATCGTAATGAAGAAGAAATAA

Upstream 100 bases:

>100_bases
CGCCAGTAACAAGCGAAGCCGGTTTGTTTTGACTTGATAAATTAGGCTGTAAATTTAATCAATAATTTCTCTTTTCACTC
TTCAACAGGAATAGCTGTTT

Downstream 100 bases:

>100_bases
CAACAAGCCTCGGGCTGGTCTTATCAAAAAAAGGCCAGCCTGTTTTTTATTTCCATGAAAGCGACTCTCCTTGCGGCGCT
GCTGCTTTTTTTGAGCTTTA

Product: ATP-dependent Clp protease, ATP-binding subunit ClpB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MNTADTRQRLLDIEKQIASLREEQATVKAQWEAEKELIHTSRRLKEELEDLRVQAENYERSGDYGKVAEIRYGKIAEIEK
ALEENNRKIEARQASGDLIMKEEIDAGDIADIVSRWTGIPVSKMLQSERQKLLGIESELHRRVVGQDEAVRAVSDAVKRS
RAGMGDEKRPIGSFIFLGPTGVGKTELARTLAEYLFDDEDALIRIDMSEYMEAHTVSRLVGAPPGYVGYEEGGQLTEAVR
RKPFSVVLLDEIEKAHPDVFNILLQILDDGRLTDSKGRTVNFKNTIIIMTSNIGAQLIQSEMEHLEGRDADAALAGLKEK
LFQLLKQQVRPEFLNRIDEVILFTPLTRENLREIVTIQFNRIKETARRQRITLEISDEALMWLAKTGFDPAFGARPLKRV
MQRQITNRLSEMILAGQVGEDDTVEIGLENDAIVMKKK

Sequences:

>Translated_438_residues
MNTADTRQRLLDIEKQIASLREEQATVKAQWEAEKELIHTSRRLKEELEDLRVQAENYERSGDYGKVAEIRYGKIAEIEK
ALEENNRKIEARQASGDLIMKEEIDAGDIADIVSRWTGIPVSKMLQSERQKLLGIESELHRRVVGQDEAVRAVSDAVKRS
RAGMGDEKRPIGSFIFLGPTGVGKTELARTLAEYLFDDEDALIRIDMSEYMEAHTVSRLVGAPPGYVGYEEGGQLTEAVR
RKPFSVVLLDEIEKAHPDVFNILLQILDDGRLTDSKGRTVNFKNTIIIMTSNIGAQLIQSEMEHLEGRDADAALAGLKEK
LFQLLKQQVRPEFLNRIDEVILFTPLTRENLREIVTIQFNRIKETARRQRITLEISDEALMWLAKTGFDPAFGARPLKRV
MQRQITNRLSEMILAGQVGEDDTVEIGLENDAIVMKKK
>Mature_438_residues
MNTADTRQRLLDIEKQIASLREEQATVKAQWEAEKELIHTSRRLKEELEDLRVQAENYERSGDYGKVAEIRYGKIAEIEK
ALEENNRKIEARQASGDLIMKEEIDAGDIADIVSRWTGIPVSKMLQSERQKLLGIESELHRRVVGQDEAVRAVSDAVKRS
RAGMGDEKRPIGSFIFLGPTGVGKTELARTLAEYLFDDEDALIRIDMSEYMEAHTVSRLVGAPPGYVGYEEGGQLTEAVR
RKPFSVVLLDEIEKAHPDVFNILLQILDDGRLTDSKGRTVNFKNTIIIMTSNIGAQLIQSEMEHLEGRDADAALAGLKEK
LFQLLKQQVRPEFLNRIDEVILFTPLTRENLREIVTIQFNRIKETARRQRITLEISDEALMWLAKTGFDPAFGARPLKRV
MQRQITNRLSEMILAGQVGEDDTVEIGLENDAIVMKKK

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family

Homologues:

Organism=Homo sapiens, GI13540606, Length=299, Percent_Identity=39.1304347826087, Blast_Score=211, Evalue=1e-54,
Organism=Escherichia coli, GI1788943, Length=427, Percent_Identity=51.5222482435597, Blast_Score=451, Evalue=1e-128,
Organism=Escherichia coli, GI1787109, Length=331, Percent_Identity=42.2960725075529, Blast_Score=276, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6320464, Length=427, Percent_Identity=45.1990632318501, Blast_Score=387, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6323002, Length=428, Percent_Identity=39.9532710280374, Blast_Score=318, Evalue=2e-87,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CLPB1_CHLTE (Q8KG79)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_660995.1
- ProteinModelPortal:   Q8KG79
- GeneID:   1006510
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT0089
- NMPDR:   fig|194439.1.peg.89
- TIGR:   CT0089
- HOGENOM:   HBG413133
- OMA:   KRSRAGM
- ProtClustDB:   CLSK637153
- BioCyc:   CTEP194439:CT_0089-MONOMER
- GO:   GO:0005737
- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR001270
- InterPro:   IPR019489
- PRINTS:   PR00300
- SMART:   SM00382

Pfam domain/function: PF07724 AAA_2; PF10431 ClpB_D2-small

EC number: NA

Molecular weight: Translated: 49655; Mature: 49655

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00870 CLPAB_1; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTADTRQRLLDIEKQIASLREEQATVKAQWEAEKELIHTSRRLKEELEDLRVQAENYER
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SGDYGKVAEIRYGKIAEIEKALEENNRKIEARQASGDLIMKEEIDAGDIADIVSRWTGIP
CCCCCCCHHCCCCHHHHHHHHHHHCCCEEEEECCCCCEEEECCCCCCHHHHHHHHHCCCC
VSKMLQSERQKLLGIESELHRRVVGQDEAVRAVSDAVKRSRAGMGDEKRPIGSFIFLGPT
HHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCC
GVGKTELARTLAEYLFDDEDALIRIDMSEYMEAHTVSRLVGAPPGYVGYEEGGQLTEAVR
CCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
RKPFSVVLLDEIEKAHPDVFNILLQILDDGRLTDSKGRTVNFKNTIIIMTSNIGAQLIQS
CCCCEEEEHHHHHHHCCHHHHHHHHHHCCCCEECCCCCEEEECCEEEEEECCHHHHHHHH
EMEHLEGRDADAALAGLKEKLFQLLKQQVRPEFLNRIDEVILFTPLTRENLREIVTIQFN
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHEEECCCCHHHHHHHHHHHHH
RIKETARRQRITLEISDEALMWLAKTGFDPAFGARPLKRVMQRQITNRLSEMILAGQVGE
HHHHHHHHCEEEEEECCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
DDTVEIGLENDAIVMKKK
CCCEEEECCCCEEEEECC
>Mature Secondary Structure
MNTADTRQRLLDIEKQIASLREEQATVKAQWEAEKELIHTSRRLKEELEDLRVQAENYER
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SGDYGKVAEIRYGKIAEIEKALEENNRKIEARQASGDLIMKEEIDAGDIADIVSRWTGIP
CCCCCCCHHCCCCHHHHHHHHHHHCCCEEEEECCCCCEEEECCCCCCHHHHHHHHHCCCC
VSKMLQSERQKLLGIESELHRRVVGQDEAVRAVSDAVKRSRAGMGDEKRPIGSFIFLGPT
HHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCC
GVGKTELARTLAEYLFDDEDALIRIDMSEYMEAHTVSRLVGAPPGYVGYEEGGQLTEAVR
CCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
RKPFSVVLLDEIEKAHPDVFNILLQILDDGRLTDSKGRTVNFKNTIIIMTSNIGAQLIQS
CCCCEEEEHHHHHHHCCHHHHHHHHHHCCCCEECCCCCEEEECCEEEEEECCHHHHHHHH
EMEHLEGRDADAALAGLKEKLFQLLKQQVRPEFLNRIDEVILFTPLTRENLREIVTIQFN
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHEEECCCCHHHHHHHHHHHHH
RIKETARRQRITLEISDEALMWLAKTGFDPAFGARPLKRVMQRQITNRLSEMILAGQVGE
HHHHHHHHCEEEEEECCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
DDTVEIGLENDAIVMKKK
CCCEEEECCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901