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The map label for this gene is amiB

Identifier: 21672815

GI number: 21672815

Start: 611377

End: 612078

Strand: Direct

Name: amiB

Synonym: BUsg555

Alternate gene names: 21672815

Gene position: 611377-612078 (Clockwise)

Preceding gene: 21672814

Following gene: 21672817

Centisome position: 95.31

GC content: 22.51

Gene sequence:

>702_bases
GTGATTGATCCAGGACATGGAGGACAAGATCCAGGAGCAATAAATAGTTTAGGATTACAAGAAAAAAAAATAACTTTAAA
GATTGGTATAAAACTTAAAAATTTATTACAAAATAGTGATTTATTTTATCCAGTTTTAACGCGAAATGATGATTCTTATG
TATCATTAAAAAAAAGAAGAGATTTTCTAAAAAATAATCACGTAAGTTTTTTAATATCTATCCATGCAGACTCTTCTAAA
AAAAGATATGTATCAGGTGCATCAATCTGGATTACTACTAATGATAGAATGCATCGTGAAATTAATAATTTTATAAAAAA
TAGAGAAGAAAACATATATTTTCCTAAAAACATTCAAAATTTAATTCAAAAAAATAAACATGATTTTTTTTTAAAAAAAA
CTGTTCTCGATTTACAATTTAATAATTTTCAAAAAATGGAAATTAATTTATCTAGATATATATTTCAACAACTTAAAAAA
ATTATAAAATTAGATAAAATAAATCTAAACTATGCCAGTTTAGGAATACTGAGCTCTATAAATACACCTTCTATGTTAAT
AGAAACAGGGTTTATCACAAATTTTTTAGAAGAAAAAAAATTAAGAACTAACAAATACCAAAATAAAATTGCAAATGCTA
TCTATATTGCTTTAAAAAATTATTTTCAAGATAGATTGCTCTCTAACTTGAGAAATACATAA

Upstream 100 bases:

>100_bases
ATAATTTTAAAAAAAATAAATATGATTTTTTTTTAAAAATATAAAAAAAAATCAAAAGAACAAGAACAATACAAAAAACC
CATTAAAAAAATTTATTATA

Downstream 100 bases:

>100_bases
AAAATTTGAAAAATAATTCTTAAAATTATTTTAAGCATCTAATTTATTGTAAGATGCTTAAAATTAAAAATTATAAATTA
AAATATCATTTATACCGTAT

Product: N-acetylmuramoyl-L-alanine amidase II precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MIDPGHGGQDPGAINSLGLQEKKITLKIGIKLKNLLQNSDLFYPVLTRNDDSYVSLKKRRDFLKNNHVSFLISIHADSSK
KRYVSGASIWITTNDRMHREINNFIKNREENIYFPKNIQNLIQKNKHDFFLKKTVLDLQFNNFQKMEINLSRYIFQQLKK
IIKLDKINLNYASLGILSSINTPSMLIETGFITNFLEEKKLRTNKYQNKIANAIYIALKNYFQDRLLSNLRNT

Sequences:

>Translated_233_residues
MIDPGHGGQDPGAINSLGLQEKKITLKIGIKLKNLLQNSDLFYPVLTRNDDSYVSLKKRRDFLKNNHVSFLISIHADSSK
KRYVSGASIWITTNDRMHREINNFIKNREENIYFPKNIQNLIQKNKHDFFLKKTVLDLQFNNFQKMEINLSRYIFQQLKK
IIKLDKINLNYASLGILSSINTPSMLIETGFITNFLEEKKLRTNKYQNKIANAIYIALKNYFQDRLLSNLRNT
>Mature_233_residues
MIDPGHGGQDPGAINSLGLQEKKITLKIGIKLKNLLQNSDLFYPVLTRNDDSYVSLKKRRDFLKNNHVSFLISIHADSSK
KRYVSGASIWITTNDRMHREINNFIKNREENIYFPKNIQNLIQKNKHDFFLKKTVLDLQFNNFQKMEINLSRYIFQQLKK
IIKLDKINLNYASLGILSSINTPSMLIETGFITNFLEEKKLRTNKYQNKIANAIYIALKNYFQDRLLSNLRNT

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family

Homologues:

Organism=Escherichia coli, GI1790611, Length=221, Percent_Identity=39.3665158371041, Blast_Score=190, Evalue=7e-50,
Organism=Escherichia coli, GI87082163, Length=226, Percent_Identity=28.7610619469027, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI1788776, Length=226, Percent_Identity=30.9734513274336, Blast_Score=112, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AMIB_BUCAP (Q8K908)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660882.1
- ProteinModelPortal:   Q8K908
- SMR:   Q8K908
- EnsemblBacteria:   EBBUCT00000000378
- GeneID:   1005636
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg555
- GeneTree:   EBGT00050000007972
- HOGENOM:   HBG656726
- OMA:   LISIHAN
- ProtClustDB:   CLSK390915
- BioCyc:   BAPH198804:BUSG555-MONOMER
- InterPro:   IPR002508
- Gene3D:   G3DSA:3.40.630.40
- SMART:   SM00646

Pfam domain/function: PF01520 Amidase_3

EC number: =3.5.1.28

Molecular weight: Translated: 27259; Mature: 27259

Theoretical pI: Translated: 10.54; Mature: 10.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDPGHGGQDPGAINSLGLQEKKITLKIGIKLKNLLQNSDLFYPVLTRNDDSYVSLKKRR
CCCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHCCCCCEEEEEECCCCCHHHHHHHH
DFLKNNHVSFLISIHADSSKKRYVSGASIWITTNDRMHREINNFIKNREENIYFPKNIQN
HHHCCCCEEEEEEEECCCCCCEEECCCEEEEEECHHHHHHHHHHHHCCCCCEECCHHHHH
LIQKNKHDFFLKKTVLDLQFNNFQKMEINLSRYIFQQLKKIIKLDKINLNYASLGILSSI
HHHCCCCCHHHHHHHEEEECCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
NTPSMLIETGFITNFLEEKKLRTNKYQNKIANAIYIALKNYFQDRLLSNLRNT
CCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIDPGHGGQDPGAINSLGLQEKKITLKIGIKLKNLLQNSDLFYPVLTRNDDSYVSLKKRR
CCCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHCCCCCEEEEEECCCCCHHHHHHHH
DFLKNNHVSFLISIHADSSKKRYVSGASIWITTNDRMHREINNFIKNREENIYFPKNIQN
HHHCCCCEEEEEEEECCCCCCEEECCCEEEEEECHHHHHHHHHHHHCCCCCEECCHHHHH
LIQKNKHDFFLKKTVLDLQFNNFQKMEINLSRYIFQQLKKIIKLDKINLNYASLGILSSI
HHHCCCCCHHHHHHHEEEECCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
NTPSMLIETGFITNFLEEKKLRTNKYQNKIANAIYIALKNYFQDRLLSNLRNT
CCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438