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The map label for this gene is trmB

Identifier: 21672796

GI number: 21672796

Start: 588018

End: 588737

Strand: Reverse

Name: trmB

Synonym: BUsg533

Alternate gene names: 21672796

Gene position: 588737-588018 (Counterclockwise)

Preceding gene: 161485723

Following gene: 30410826

Centisome position: 91.78

GC content: 22.22

Gene sequence:

>720_bases
ATGAAAAATAATATTATCACACCTCAATATAATTGCAATGGTACTTTTTTACGTAAAAATCGTAGTTTTGTTTGTCGAAA
AGGTCGAATTACTAAATCTCAATTACAGGCAATTGAAAAATATTGGTTGTCAATTGGTATTGATTTTAAATTAGAACCGT
TAGATTTTACATCTGTTTTTAAAAATAGTGCTCCTGTTATTTTGGAAATTGGTTTTGGATCAGGTGAGTCTTTAGTTAAA
ACTGCTGCTAATTTTCCAAACAAAAACTTTTTAGGAATAGAAGTTTATAAATCAGGAATAGGATCTTGTTTACGTTTAGC
TCATTATTCTGGAATTAATAATTTAAAAATTATTTATCATGATGCAATTGAAGTAATTGATCAGATGATTTTAGATCATA
CTTTATCAAAAGTACAAATTTTTTTTCCAGATCCATGGAATAAAAAAAGACATCATAAAAGAAGAATGATACAAGATTTT
TTTCTAATAAAAATTTTAAAAAAATTAAATAATGATGGTATTTTACATATTGTTACTGATTCAAAAGAATATAATTTTTT
TATATTAAACTTAATACAAAACATTGATAATTATATAAATTTATCAAAAAAAAGAATGTGTTTTGAACATTTTAAATATC
GTTTAGTAACTAACTTTGAAAAAAAAGCAAAATTATCAGGCAATAAAATATTCGATTTAATATTTAAATTAAAAAAATAG

Upstream 100 bases:

>100_bases
ATTTTTTAATTTTAAAAATTTTAAAAAATATGAGATATAAATTATCTTAATGTAATTGACTTCAATATTTTTTACTTGAT
TAAAAAAAGTATGATTAAAT

Downstream 100 bases:

>100_bases
AATTTTTTAATCTAAAAAAATCTTTAATAATGAATTAAGAAACATTTTTCCTTTTTTTGTTGTATCCCAAAAATCAATTT
TATTTTTTAGGTATCCTTTT

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MKNNIITPQYNCNGTFLRKNRSFVCRKGRITKSQLQAIEKYWLSIGIDFKLEPLDFTSVFKNSAPVILEIGFGSGESLVK
TAANFPNKNFLGIEVYKSGIGSCLRLAHYSGINNLKIIYHDAIEVIDQMILDHTLSKVQIFFPDPWNKKRHHKRRMIQDF
FLIKILKKLNNDGILHIVTDSKEYNFFILNLIQNIDNYINLSKKRMCFEHFKYRLVTNFEKKAKLSGNKIFDLIFKLKK

Sequences:

>Translated_239_residues
MKNNIITPQYNCNGTFLRKNRSFVCRKGRITKSQLQAIEKYWLSIGIDFKLEPLDFTSVFKNSAPVILEIGFGSGESLVK
TAANFPNKNFLGIEVYKSGIGSCLRLAHYSGINNLKIIYHDAIEVIDQMILDHTLSKVQIFFPDPWNKKRHHKRRMIQDF
FLIKILKKLNNDGILHIVTDSKEYNFFILNLIQNIDNYINLSKKRMCFEHFKYRLVTNFEKKAKLSGNKIFDLIFKLKK
>Mature_239_residues
MKNNIITPQYNCNGTFLRKNRSFVCRKGRITKSQLQAIEKYWLSIGIDFKLEPLDFTSVFKNSAPVILEIGFGSGESLVK
TAANFPNKNFLGIEVYKSGIGSCLRLAHYSGINNLKIIYHDAIEVIDQMILDHTLSKVQIFFPDPWNKKRHHKRRMIQDF
FLIKILKKLNNDGILHIVTDSKEYNFFILNLIQNIDNYINLSKKRMCFEHFKYRLVTNFEKKAKLSGNKIFDLIFKLKK

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=236, Percent_Identity=45.3389830508475, Blast_Score=234, Evalue=4e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_BUCAP (Q8K927)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660863.1
- ProteinModelPortal:   Q8K927
- SMR:   Q8K927
- EnsemblBacteria:   EBBUCT00000000318
- GeneID:   1005542
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg533
- GeneTree:   EBGT00050000008077
- HOGENOM:   HBG322782
- OMA:   HELEGYH
- ProtClustDB:   PRK00121
- BioCyc:   BAPH198804:BUSG533-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 27959; Mature: 27959

Theoretical pI: Translated: 10.42; Mature: 10.42

Prosite motif: NA

Important sites: BINDING 69-69 BINDING 94-94 BINDING 121-121 BINDING 144-144 BINDING 148-148 BINDING 180-180

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNNIITPQYNCNGTFLRKNRSFVCRKGRITKSQLQAIEKYWLSIGIDFKLEPLDFTSVF
CCCCCCCCCCCCCEEEEECCCCEEEECCCCCHHHHHHHHHHHHHCCCCEEECCCCHHHHH
KNSAPVILEIGFGSGESLVKTAANFPNKNFLGIEVYKSGIGSCLRLAHYSGINNLKIIYH
CCCCCEEEEEECCCCHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEH
DAIEVIDQMILDHTLSKVQIFFPDPWNKKRHHKRRMIQDFFLIKILKKLNNDGILHIVTD
HHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
SKEYNFFILNLIQNIDNYINLSKKRMCFEHFKYRLVTNFEKKAKLSGNKIFDLIFKLKK
CCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKNNIITPQYNCNGTFLRKNRSFVCRKGRITKSQLQAIEKYWLSIGIDFKLEPLDFTSVF
CCCCCCCCCCCCCEEEEECCCCEEEECCCCCHHHHHHHHHHHHHCCCCEEECCCCHHHHH
KNSAPVILEIGFGSGESLVKTAANFPNKNFLGIEVYKSGIGSCLRLAHYSGINNLKIIYH
CCCCCEEEEEECCCCHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEH
DAIEVIDQMILDHTLSKVQIFFPDPWNKKRHHKRRMIQDFFLIKILKKLNNDGILHIVTD
HHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
SKEYNFFILNLIQNIDNYINLSKKRMCFEHFKYRLVTNFEKKAKLSGNKIFDLIFKLKK
CCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438