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The map label for this gene is yhhP [H]

Identifier: 21672697

GI number: 21672697

Start: 489502

End: 489762

Strand: Direct

Name: yhhP [H]

Synonym: BUsg432

Alternate gene names: 21672697

Gene position: 489502-489762 (Clockwise)

Preceding gene: 21672696

Following gene: 21672699

Centisome position: 76.31

GC content: 24.14

Gene sequence:

>261_bases
TTGATTTCTATTTCTTTAAAAAAAACAAAATATGAAAAAAAAAATATTATATTAAATTTAATTGGACTTCGATGTCCAGA
ACCTATTATGGTAATAAGAAAAACATTAAGAAAAATGAAAAAAAATGAAAAGATACTAGTATTAGCAGATGATCCATCAA
CGAAAAGAGATATTCCTTATTTTTGTTATTTTATGGAACATCAACTTCTAGATCGTTTCATTCATGTAAAACCTTATCGC
TACTTACTAAAAAAGGGATGA

Upstream 100 bases:

>100_bases
GGAAAAGTTATTACGAAGGTAAAAGCGCTTGTGGATTATTACCTTCAAAAATAGATAAATATATTTTATCAAAAAATCTA
TATAAAATTTATAATCAAAA

Downstream 100 bases:

>100_bases
TCTTCTACTAAGATAGATTAAATGAATCAATACAATTTATGTAAAAAAATCAAAAGATTAATATACACACTGGGTGTGTA
TATTAATATATACTATATAA

Product: sulfur transfer protein SirA

Products: NA

Alternate protein names: tRNA 2-thiouridine synthesizing protein A [H]

Number of amino acids: Translated: 86; Mature: 86

Protein sequence:

>86_residues
MISISLKKTKYEKKNIILNLIGLRCPEPIMVIRKTLRKMKKNEKILVLADDPSTKRDIPYFCYFMEHQLLDRFIHVKPYR
YLLKKG

Sequences:

>Translated_86_residues
MISISLKKTKYEKKNIILNLIGLRCPEPIMVIRKTLRKMKKNEKILVLADDPSTKRDIPYFCYFMEHQLLDRFIHVKPYR
YLLKKG
>Mature_86_residues
MISISLKKTKYEKKNIILNLIGLRCPEPIMVIRKTLRKMKKNEKILVLADDPSTKRDIPYFCYFMEHQLLDRFIHVKPYR
YLLKKG

Specific function: Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions. Interacts with iscS and stimulates its activity. Then, accepts a sulfur from iscS and transfers it in turn to tusD [H]

COG id: COG0425

COG function: function code O; Predicted redox protein, regulator of disulfide bond formation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0033 family. TusA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789881, Length=76, Percent_Identity=51.3157894736842, Blast_Score=97, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001455
- InterPro:   IPR022931 [H]

Pfam domain/function: PF01206 SirA [H]

EC number: NA

Molecular weight: Translated: 10369; Mature: 10369

Theoretical pI: Translated: 10.57; Mature: 10.57

Prosite motif: PS01148 UPF0033

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISISLKKTKYEKKNIILNLIGLRCPEPIMVIRKTLRKMKKNEKILVLADDPSTKRDIPY
CEEECCCCCCCCHHHHHEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCH
FCYFMEHQLLDRFIHVKPYRYLLKKG
HHHHHHHHHHHHHHCCCHHHHHHCCC
>Mature Secondary Structure
MISISLKKTKYEKKNIILNLIGLRCPEPIMVIRKTLRKMKKNEKILVLADDPSTKRDIPY
CEEECCCCCCCCHHHHHEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCH
FCYFMEHQLLDRFIHVKPYRYLLKKG
HHHHHHHHHHHHHHCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438 [H]