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The map label for this gene is ygfZ [C]

Identifier: 21672687

GI number: 21672687

Start: 475410

End: 476369

Strand: Reverse

Name: ygfZ [C]

Synonym: BUsg420

Alternate gene names: 21672687

Gene position: 476369-475410 (Counterclockwise)

Preceding gene: 21672698

Following gene: 21672686

Centisome position: 74.26

GC content: 23.54

Gene sequence:

>960_bases
ATGTCATTATCTAAATGTTTTAAAAATTCTATTTATCCTTCAATTAATTTACCTTTAACATTAAGTTTACTTGAAGAATG
GTGTTTAATTTATATATGTGGTATTGATAGTAAAAAACATCTTCAAAATCAATTTACTATTGATATAAATTGTTTAAAAA
AAGAAGAATATAAGCTTTGTGCTCATTGTAATTTTAATGGAAAAGTATGGGCAACTATGTTTATTTTTCATTATAAAAAA
GGTTTTGCTTATATTATTCGTAAAAGCATCGCTAAAATACAAATTAAAGAATTAAAAAAATATGCTATTTTTTCTAAAGT
AGAAATTCGAGAGTTAGATGACATTTATTTATTTGGACTTTCTGGGTTTAATGCTAAGTTTTTTTTATCAAAAAATTTTG
TTGATATACCGAACAAAAATTGTTCATTAATTTCTAATCAGGATAGGACAATACTTTGGTTTTCAGAACCTTGTGAAAGA
TTTTTGTTAGTTTTATCTTTAAAAGACTTACTGCTTTTAAAGAGAAAAGAAAACGAAATAACCTTGCTGAATAACAGTAA
ACAATGGCTTCTATTTGATATAGAAGCAGGGTTTCCTATTATTGACAAACAAACTTCTCAAAAATTTTTGCCGCAATCTA
TTAATTTAATACTGCTTCAAGCTGTAAGTTTCGATAAAGGATGTTATTATGGACAGGAAACAATAGCACGAGTATTTTAT
AAAAATTTAAATAAATATTCACTATATCTTTTATCAAGTAAAGGAAATATTAATCCTAAAATTGGTTCTATTATTGAAAT
GAAAAAAGAAGAAAAATGGTATAGAATTGGCTTCTTACTAGCAATCGTTCATGTTGAATTTAATAGAACTGTTATACAAG
CTGTATTAAATAAATCTATAAATATTCAAAACATATTCAGAATTTATGAATTTAAAAATATCTTTTTAATTAAAAATTAG

Upstream 100 bases:

>100_bases
TTATAAAAATGATTAAAAAAAATTTATTTTTTTATTATATATTTAATTTGATAATTTTTTTTTTTTTTAGTTAATATATT
TTAACTTTTTAAGGAAAGTC

Downstream 100 bases:

>100_bases
TATTTTTTATTTAATGATTTTTTTTTAAAAAAAATTCTTTTTTTTGCAAAAATTTAGATAATATTGTTTAAAAAATCATG
TCTCAAGGTATTCTTTTTAT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MSLSKCFKNSIYPSINLPLTLSLLEEWCLIYICGIDSKKHLQNQFTIDINCLKKEEYKLCAHCNFNGKVWATMFIFHYKK
GFAYIIRKSIAKIQIKELKKYAIFSKVEIRELDDIYLFGLSGFNAKFFLSKNFVDIPNKNCSLISNQDRTILWFSEPCER
FLLVLSLKDLLLLKRKENEITLLNNSKQWLLFDIEAGFPIIDKQTSQKFLPQSINLILLQAVSFDKGCYYGQETIARVFY
KNLNKYSLYLLSSKGNINPKIGSIIEMKKEEKWYRIGFLLAIVHVEFNRTVIQAVLNKSINIQNIFRIYEFKNIFLIKN

Sequences:

>Translated_319_residues
MSLSKCFKNSIYPSINLPLTLSLLEEWCLIYICGIDSKKHLQNQFTIDINCLKKEEYKLCAHCNFNGKVWATMFIFHYKK
GFAYIIRKSIAKIQIKELKKYAIFSKVEIRELDDIYLFGLSGFNAKFFLSKNFVDIPNKNCSLISNQDRTILWFSEPCER
FLLVLSLKDLLLLKRKENEITLLNNSKQWLLFDIEAGFPIIDKQTSQKFLPQSINLILLQAVSFDKGCYYGQETIARVFY
KNLNKYSLYLLSSKGNINPKIGSIIEMKKEEKWYRIGFLLAIVHVEFNRTVIQAVLNKSINIQNIFRIYEFKNIFLIKN
>Mature_318_residues
SLSKCFKNSIYPSINLPLTLSLLEEWCLIYICGIDSKKHLQNQFTIDINCLKKEEYKLCAHCNFNGKVWATMFIFHYKKG
FAYIIRKSIAKIQIKELKKYAIFSKVEIRELDDIYLFGLSGFNAKFFLSKNFVDIPNKNCSLISNQDRTILWFSEPCERF
LLVLSLKDLLLLKRKENEITLLNNSKQWLLFDIEAGFPIIDKQTSQKFLPQSINLILLQAVSFDKGCYYGQETIARVFYK
NLNKYSLYLLSSKGNINPKIGSIIEMKKEEKWYRIGFLLAIVHVEFNRTVIQAVLNKSINIQNIFRIYEFKNIFLIKN

Specific function: Folate-binding protein involved in regulating the level of ATP-dnaA and in the modification of some tRNAs. It is probably a key factor in regulatory networks that act via tRNA modification, such as initiation of chromosomal replication

COG id: COG0354

COG function: function code R; Predicted aminomethyltransferase related to GcvT

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-modifying ygfZ family

Homologues:

Organism=Escherichia coli, GI1789265, Length=305, Percent_Identity=40.9836065573771, Blast_Score=263, Evalue=1e-71,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): YGFZ_BUCAP (Q8K9C6)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660754.1
- ProteinModelPortal:   Q8K9C6
- SMR:   Q8K9C6
- EnsemblBacteria:   EBBUCT00000000445
- GeneID:   1005582
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg420
- GeneTree:   EBGT00050000007842
- HOGENOM:   HBG700628
- OMA:   GAHCDPK
- ProtClustDB:   CLSK316173
- BioCyc:   BAPH198804:BUSG420-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01175
- InterPro:   IPR017703
- InterPro:   IPR006222
- TIGRFAMs:   TIGR03317

Pfam domain/function: PF01571 GCV_T

EC number: NA

Molecular weight: Translated: 37467; Mature: 37335

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: BINDING 27-27 BINDING 189-189

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSKCFKNSIYPSINLPLTLSLLEEWCLIYICGIDSKKHLQNQFTIDINCLKKEEYKLC
CCHHHHHHHCCCCCCCCCEEHHHHHCCEEEEEECCCCHHHHCCEEEEEEEEECCCCEEEE
AHCNFNGKVWATMFIFHYKKGFAYIIRKSIAKIQIKELKKYAIFSKVEIRELDDIYLFGL
EEECCCCEEEEEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCEEEEEEE
SGFNAKFFLSKNFVDIPNKNCSLISNQDRTILWFSEPCERFLLVLSLKDLLLLKRKENEI
CCCCEEEEEECCEEECCCCCCEEEECCCCEEEEECCHHHHHHHHHHHHHHHHEEECCCCE
TLLNNSKQWLLFDIEAGFPIIDKQTSQKFLPQSINLILLQAVSFDKGCYYGQETIARVFY
EEEECCCEEEEEEECCCCCEECCCCHHHCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHH
KNLNKYSLYLLSSKGNINPKIGSIIEMKKEEKWYRIGFLLAIVHVEFNRTVIQAVLNKSI
HCCCEEEEEEEECCCCCCCCCCHHHHHHHCCCHHHHEEEEEEEEEECCHHHHHHHHCCCC
NIQNIFRIYEFKNIFLIKN
CHHHHHHHEEECEEEEEEC
>Mature Secondary Structure 
SLSKCFKNSIYPSINLPLTLSLLEEWCLIYICGIDSKKHLQNQFTIDINCLKKEEYKLC
CHHHHHHHCCCCCCCCCEEHHHHHCCEEEEEECCCCHHHHCCEEEEEEEEECCCCEEEE
AHCNFNGKVWATMFIFHYKKGFAYIIRKSIAKIQIKELKKYAIFSKVEIRELDDIYLFGL
EEECCCCEEEEEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCEEEEEEE
SGFNAKFFLSKNFVDIPNKNCSLISNQDRTILWFSEPCERFLLVLSLKDLLLLKRKENEI
CCCCEEEEEECCEEECCCCCCEEEECCCCEEEEECCHHHHHHHHHHHHHHHHEEECCCCE
TLLNNSKQWLLFDIEAGFPIIDKQTSQKFLPQSINLILLQAVSFDKGCYYGQETIARVFY
EEEECCCEEEEEEECCCCCEECCCCHHHCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHH
KNLNKYSLYLLSSKGNINPKIGSIIEMKKEEKWYRIGFLLAIVHVEFNRTVIQAVLNKSI
HCCCEEEEEEEECCCCCCCCCCHHHHHHHCCCHHHHEEEEEEEEEECCHHHHHHHHCCCC
NIQNIFRIYEFKNIFLIKN
CHHHHHHHEEECEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438