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The map label for this gene is gmk

Identifier: 21672686

GI number: 21672686

Start: 474709

End: 475332

Strand: Reverse

Name: gmk

Synonym: BUsg419

Alternate gene names: 21672686

Gene position: 475332-474709 (Counterclockwise)

Preceding gene: 21672687

Following gene: 21672684

Centisome position: 74.1

GC content: 25.48

Gene sequence:

>624_bases
ATGTCTCAAGGTATTCTTTTTATTATTTCAGCACCAAGTGGAACAGGAAAATCTAGCTTAATTGAAGGATTATTAAAAAC
AAAATTTTTATATAATATTCAAGTTTCTATTTCTCATACTACTAGAGTTATGAGACCTGGTGAATCTCATGGAAAACACT
ATTATTTTATATCAAAAAAAGAATTCAGAATAATGATTAAACAAGAATCTTTTTTAGAATATGCTAAAGTGTTTAATAAC
TATTATGGAACTTCACGTCAATCTATTGAAAAAATGTTGCTTTCAGGGATTGATGTTTTTCTTGATATAGATTGGCAAGG
AGCTAATCAGATTCGGTATAAAATGCCAAATTCGAAGAGTATTTTTTTGCTTCCTCCATCTAAAGATGAATTATATAAAA
GATTAAGAGAACGAGGACAAGATAGTGATACAGTTATTTCAAAAAGAATGGAAAAAGCTGTTGATGAAATGAATCATTAT
TCAGAATATGACTATTTAATTATTAATGATGATTTTCAAAAAGCTATAAATGATTTAAGAACTATTATTATTGCTGAGCA
TTTATGTTTATTTCATCAAAAAAATAAGCATAATGTATTAATTTCTCAATTATTAAAAAGTTAA

Upstream 100 bases:

>100_bases
ATATCTTTTTAATTAAAAATTAGTATTTTTTATTTAATGATTTTTTTTTAAAAAAAATTCTTTTTTTTGCAAAAATTTAG
ATAATATTGTTTAAAAAATC

Downstream 100 bases:

>100_bases
AACTATAAAATTTTTTCTGTATGAAATATTTTTTTTATTTAAAATTATTTATATAATTCTATTGAGAAAATAGAATTATA
TAATTAATAAAAGTTGATTT

Product: guanylate kinase

Products: NA

Alternate protein names: GMP kinase

Number of amino acids: Translated: 207; Mature: 206

Protein sequence:

>207_residues
MSQGILFIISAPSGTGKSSLIEGLLKTKFLYNIQVSISHTTRVMRPGESHGKHYYFISKKEFRIMIKQESFLEYAKVFNN
YYGTSRQSIEKMLLSGIDVFLDIDWQGANQIRYKMPNSKSIFLLPPSKDELYKRLRERGQDSDTVISKRMEKAVDEMNHY
SEYDYLIINDDFQKAINDLRTIIIAEHLCLFHQKNKHNVLISQLLKS

Sequences:

>Translated_207_residues
MSQGILFIISAPSGTGKSSLIEGLLKTKFLYNIQVSISHTTRVMRPGESHGKHYYFISKKEFRIMIKQESFLEYAKVFNN
YYGTSRQSIEKMLLSGIDVFLDIDWQGANQIRYKMPNSKSIFLLPPSKDELYKRLRERGQDSDTVISKRMEKAVDEMNHY
SEYDYLIINDDFQKAINDLRTIIIAEHLCLFHQKNKHNVLISQLLKS
>Mature_206_residues
SQGILFIISAPSGTGKSSLIEGLLKTKFLYNIQVSISHTTRVMRPGESHGKHYYFISKKEFRIMIKQESFLEYAKVFNNY
YGTSRQSIEKMLLSGIDVFLDIDWQGANQIRYKMPNSKSIFLLPPSKDELYKRLRERGQDSDTVISKRMEKAVDEMNHYS
EYDYLIINDDFQKAINDLRTIIIAEHLCLFHQKNKHNVLISQLLKS

Specific function: Essential for recycling GMP and indirectly, cGMP

COG id: COG0194

COG function: function code F; Guanylate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 guanylate kinase-like domain

Homologues:

Organism=Homo sapiens, GI226874833, Length=190, Percent_Identity=33.6842105263158, Blast_Score=106, Evalue=1e-23,
Organism=Homo sapiens, GI4504221, Length=190, Percent_Identity=33.6842105263158, Blast_Score=106, Evalue=1e-23,
Organism=Homo sapiens, GI226874831, Length=190, Percent_Identity=33.6842105263158, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI111154074, Length=165, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=3e-14,
Organism=Homo sapiens, GI38570142, Length=185, Percent_Identity=28.6486486486486, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI21361598, Length=192, Percent_Identity=28.6458333333333, Blast_Score=68, Evalue=5e-12,
Organism=Homo sapiens, GI14780902, Length=188, Percent_Identity=23.4042553191489, Blast_Score=65, Evalue=3e-11,
Organism=Escherichia coli, GI1790080, Length=205, Percent_Identity=64.8780487804878, Blast_Score=295, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI17508835, Length=172, Percent_Identity=36.6279069767442, Blast_Score=120, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI193203132, Length=172, Percent_Identity=36.6279069767442, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6320662, Length=191, Percent_Identity=40.8376963350785, Blast_Score=136, Evalue=3e-33,
Organism=Drosophila melanogaster, GI21356141, Length=173, Percent_Identity=38.150289017341, Blast_Score=120, Evalue=5e-28,
Organism=Drosophila melanogaster, GI45554481, Length=174, Percent_Identity=26.4367816091954, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI85724806, Length=174, Percent_Identity=26.4367816091954, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI116007136, Length=174, Percent_Identity=26.4367816091954, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24640542, Length=174, Percent_Identity=26.4367816091954, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI45554489, Length=174, Percent_Identity=26.4367816091954, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI161077626, Length=174, Percent_Identity=26.4367816091954, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KGUA_BUCAP (Q8K9C7)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660753.1
- ProteinModelPortal:   Q8K9C7
- SMR:   Q8K9C7
- EnsemblBacteria:   EBBUCT00000000473
- GeneID:   1005742
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg419
- GeneTree:   EBGT00050000008057
- HOGENOM:   HBG671982
- OMA:   DIDWQGT
- ProtClustDB:   PRK00300
- BioCyc:   BAPH198804:BUSG419-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00328
- InterPro:   IPR008144
- InterPro:   IPR008145
- InterPro:   IPR020590
- InterPro:   IPR017665
- SMART:   SM00072
- TIGRFAMs:   TIGR03263

Pfam domain/function: PF00625 Guanylate_kin

EC number: =2.7.4.8

Molecular weight: Translated: 24153; Mature: 24022

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS00856 GUANYLATE_KINASE_1; PS50052 GUANYLATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQGILFIISAPSGTGKSSLIEGLLKTKFLYNIQVSISHTTRVMRPGESHGKHYYFISKK
CCCCEEEEEECCCCCCHHHHHHHHHHHHEEEEEEEEEECCEEEECCCCCCCCEEEEEECC
EFRIMIKQESFLEYAKVFNNYYGTSRQSIEKMLLSGIDVFLDIDWQGANQIRYKMPNSKS
EEEEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEECCCCCCEEEEECCCCCE
IFLLPPSKDELYKRLRERGQDSDTVISKRMEKAVDEMNHYSEYDYLIINDDFQKAINDLR
EEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHH
TIIIAEHLCLFHQKNKHNVLISQLLKS
HHHHHHHHHHHHCCCCCHHHHHHHHCC
>Mature Secondary Structure 
SQGILFIISAPSGTGKSSLIEGLLKTKFLYNIQVSISHTTRVMRPGESHGKHYYFISKK
CCCEEEEEECCCCCCHHHHHHHHHHHHEEEEEEEEEECCEEEECCCCCCCCEEEEEECC
EFRIMIKQESFLEYAKVFNNYYGTSRQSIEKMLLSGIDVFLDIDWQGANQIRYKMPNSKS
EEEEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEECCCCCCEEEEECCCCCE
IFLLPPSKDELYKRLRERGQDSDTVISKRMEKAVDEMNHYSEYDYLIINDDFQKAINDLR
EEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHH
TIIIAEHLCLFHQKNKHNVLISQLLKS
HHHHHHHHHHHHCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438