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The map label for this gene is pyrG

Identifier: 21672672

GI number: 21672672

Start: 451890

End: 453551

Strand: Direct

Name: pyrG

Synonym: BUsg399

Alternate gene names: 21672672

Gene position: 451890-453551 (Clockwise)

Preceding gene: 21672671

Following gene: 21672673

Centisome position: 70.45

GC content: 27.26

Gene sequence:

>1662_bases
ATGACTAAAAATTATATTTTTATAACTGGCGGCGTAGTATCATCTTTAGGAAAGGGTATTGCAGCTGCTTCATTGGGAGC
TGTATTAGAAGCTCGAAATTTAAAAATAACTATTATGAAATTAGATCCATATATTAATGTTGATCCAGGAACAATGAGTC
CTATTCAACATGGAGAAGTCTTTGTTACTGAAGATGGTGCGGAAACAGATCTAGATTTAGGTCATTATGAACGTTTTATT
CGAACAAAAATGACATGTTTAAATAATTTTACTACTGGAAGTATTTATTCTGAAGTTTTAAAAAAAGAAAGAAGAGGTGA
TTATTTAGGTTCAACTATTCAAGTAATACCGCATATTACTAATGCTATTAAAGATAGAATAATTTTATGCTCTAAAAATA
GCGATATTATCCTTGTCGAAATAGGTGGGACAGTTGGAGATATTGAATCTTTACCATTTCTTGAAGCAATTCGTCAACTA
GCCGTGGACATTGGGCGTAAAAATGTAATATACATACATTTAACACTTGTACCGTATATAAAAACAGCTGGAGAAATTAA
AACTAAACCTACTCAACATTCAGTAAAAGAATTGCTATCAATTGGAATACAACCAGATATTTTGATTTGTCGTTCTCAAA
AAACAGTTCCAATAAACGAAAGAAAAAAAATTGCATTATTTTGCAATGTTCCAGTGAATGCTGTAATCTCTTTAAAAGAC
GTAGATTCGATATACACAATACCAAAACTGTTAAAAGATCAAAAATTAGATAATTATATTTGCGAATATTTTAAATTAAA
TGTCCCTCAAGCTGATTTAAAAGAATGGGAAAAAGTTATTTATGAAGAAAAAAATGCTAGTAAAGAAATTATTATTGGTA
TAATAGGAAAATATATAAAATTGCCTGATGCATATAAATCAGTAATAGAAGCACTTAAACATGCAGGATTAAAAAATAAA
ATTAAAGTAAAAATAGAGTTAATTAACTCTCAAGAAATAGAAAATAAAAATTTTAAATTATTACAAAATCTTAATGGTAT
TCTAATACCAGGTGGTTTTGGAGATCGGGGTATAATAGGAAAATTATTATCTGTTCAATACGCACGTGAAAATAATATTC
CTTATTTCGGAATATGCTTAGGAATGCAAATAGCAATCATAGAATTCGCACAAAATGTTATAGGAATTAAAGAAGCGAAT
TCTACAGAATTTGATCCACAATGTAAATTTCCCGTGATCGATTTAATTAAAACTAAAAAAAATGATATAAAAGATACAAG
ACAAGGAAAAAAAAATAATAAATCTAATTTTGGAGGTACTATGAGACTAGGAAGTCAACCGTGCAAATTAATTTTTAATA
GTTTGTCTAGAAAATTATATAAAAAAGATACCATTATAGAACGGCATAGACATCGATATGAAGTAAACAATTTTTTATTA
AAAAAAATAGAAAAAAATGGATTAAAAATAGCAGGACGTTCTAAAAAAAATAATATAGTTGAAATCATAGAAATTTTTGA
TCATCCATGGTTTATTGGTTGTCAATTTCATCCTGAATTTACTTCTACACCGCGCGATGGACATCCATTATTCATAGATT
TTATTAAATCAGCAAAAAAAAATAAAAAAAATAATTTTAAAATAAAGGTAAAAAATGTCTAA

Upstream 100 bases:

>100_bases
TATTTGAAAATCTATTGAATTTATTTTACTAAAATAATTTAATTAAATATTAAAATTTTAAAACTATCTATTCATTTTAA
CTGATATTTTTATATTTCGC

Downstream 100 bases:

>100_bases
AATATTAAAAATAATTGGTCGTGAAATCATCGATTCTAGAGGTAATCCTACCATAGAATGTGAAGTTTTGCTTGAAGGAG
GTTTTGTTGGTTTAGCTTCA

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 553; Mature: 552

Protein sequence:

>553_residues
MTKNYIFITGGVVSSLGKGIAAASLGAVLEARNLKITIMKLDPYINVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFI
RTKMTCLNNFTTGSIYSEVLKKERRGDYLGSTIQVIPHITNAIKDRIILCSKNSDIILVEIGGTVGDIESLPFLEAIRQL
AVDIGRKNVIYIHLTLVPYIKTAGEIKTKPTQHSVKELLSIGIQPDILICRSQKTVPINERKKIALFCNVPVNAVISLKD
VDSIYTIPKLLKDQKLDNYICEYFKLNVPQADLKEWEKVIYEEKNASKEIIIGIIGKYIKLPDAYKSVIEALKHAGLKNK
IKVKIELINSQEIENKNFKLLQNLNGILIPGGFGDRGIIGKLLSVQYARENNIPYFGICLGMQIAIIEFAQNVIGIKEAN
STEFDPQCKFPVIDLIKTKKNDIKDTRQGKKNNKSNFGGTMRLGSQPCKLIFNSLSRKLYKKDTIIERHRHRYEVNNFLL
KKIEKNGLKIAGRSKKNNIVEIIEIFDHPWFIGCQFHPEFTSTPRDGHPLFIDFIKSAKKNKKNNFKIKVKNV

Sequences:

>Translated_553_residues
MTKNYIFITGGVVSSLGKGIAAASLGAVLEARNLKITIMKLDPYINVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFI
RTKMTCLNNFTTGSIYSEVLKKERRGDYLGSTIQVIPHITNAIKDRIILCSKNSDIILVEIGGTVGDIESLPFLEAIRQL
AVDIGRKNVIYIHLTLVPYIKTAGEIKTKPTQHSVKELLSIGIQPDILICRSQKTVPINERKKIALFCNVPVNAVISLKD
VDSIYTIPKLLKDQKLDNYICEYFKLNVPQADLKEWEKVIYEEKNASKEIIIGIIGKYIKLPDAYKSVIEALKHAGLKNK
IKVKIELINSQEIENKNFKLLQNLNGILIPGGFGDRGIIGKLLSVQYARENNIPYFGICLGMQIAIIEFAQNVIGIKEAN
STEFDPQCKFPVIDLIKTKKNDIKDTRQGKKNNKSNFGGTMRLGSQPCKLIFNSLSRKLYKKDTIIERHRHRYEVNNFLL
KKIEKNGLKIAGRSKKNNIVEIIEIFDHPWFIGCQFHPEFTSTPRDGHPLFIDFIKSAKKNKKNNFKIKVKNV
>Mature_552_residues
TKNYIFITGGVVSSLGKGIAAASLGAVLEARNLKITIMKLDPYINVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIR
TKMTCLNNFTTGSIYSEVLKKERRGDYLGSTIQVIPHITNAIKDRIILCSKNSDIILVEIGGTVGDIESLPFLEAIRQLA
VDIGRKNVIYIHLTLVPYIKTAGEIKTKPTQHSVKELLSIGIQPDILICRSQKTVPINERKKIALFCNVPVNAVISLKDV
DSIYTIPKLLKDQKLDNYICEYFKLNVPQADLKEWEKVIYEEKNASKEIIIGIIGKYIKLPDAYKSVIEALKHAGLKNKI
KVKIELINSQEIENKNFKLLQNLNGILIPGGFGDRGIIGKLLSVQYARENNIPYFGICLGMQIAIIEFAQNVIGIKEANS
TEFDPQCKFPVIDLIKTKKNDIKDTRQGKKNNKSNFGGTMRLGSQPCKLIFNSLSRKLYKKDTIIERHRHRYEVNNFLLK
KIEKNGLKIAGRSKKNNIVEIIEIFDHPWFIGCQFHPEFTSTPRDGHPLFIDFIKSAKKNKKNNFKIKVKNV

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI148491070, Length=561, Percent_Identity=42.602495543672, Blast_Score=472, Evalue=1e-133,
Organism=Homo sapiens, GI28559085, Length=566, Percent_Identity=43.1095406360424, Blast_Score=471, Evalue=1e-132,
Organism=Homo sapiens, GI28559083, Length=566, Percent_Identity=43.1095406360424, Blast_Score=471, Evalue=1e-132,
Organism=Homo sapiens, GI221316689, Length=566, Percent_Identity=43.1095406360424, Blast_Score=471, Evalue=1e-132,
Organism=Escherichia coli, GI1789142, Length=547, Percent_Identity=68.1901279707495, Blast_Score=787, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25148299, Length=617, Percent_Identity=35.4943273905997, Blast_Score=398, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6322563, Length=551, Percent_Identity=41.3793103448276, Blast_Score=442, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6319432, Length=569, Percent_Identity=40.597539543058, Blast_Score=442, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24664469, Length=561, Percent_Identity=41.5329768270945, Blast_Score=457, Evalue=1e-129,
Organism=Drosophila melanogaster, GI21357815, Length=507, Percent_Identity=40.2366863905325, Blast_Score=391, Evalue=1e-109,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_BUCAP (P59039)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660739.1
- ProteinModelPortal:   P59039
- SMR:   P59039
- EnsemblBacteria:   EBBUCT00000000163
- GeneID:   1005761
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg399
- GeneTree:   EBGT00050000008171
- HOGENOM:   HBG597806
- OMA:   RVTMQKL
- ProtClustDB:   PRK05380
- BioCyc:   BAPH198804:BUSG399-MONOMER
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 62454; Mature: 62323

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 379-379 ACT_SITE 517-517 ACT_SITE 519-519

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKNYIFITGGVVSSLGKGIAAASLGAVLEARNLKITIMKLDPYINVDPGTMSPIQHGEV
CCCCEEEEECHHHHHHCCCHHHHHHHHHHEECCCEEEEEEECCEEECCCCCCCCCCCCEE
FVTEDGAETDLDLGHYERFIRTKMTCLNNFTTGSIYSEVLKKERRGDYLGSTIQVIPHIT
EEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHH
NAIKDRIILCSKNSDIILVEIGGTVGDIESLPFLEAIRQLAVDIGRKNVIYIHLTLVPYI
HHHHCEEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEHHH
KTAGEIKTKPTQHSVKELLSIGIQPDILICRSQKTVPINERKKIALFCNVPVNAVISLKD
HCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCEEEEEECCCCCEEEEECC
VDSIYTIPKLLKDQKLDNYICEYFKLNVPQADLKEWEKVIYEEKNASKEIIIGIIGKYIK
CHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEHHEEHHHHHC
LPDAYKSVIEALKHAGLKNKIKVKIELINSQEIENKNFKLLQNLNGILIPGGFGDRGIIG
CCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCHHHHHCCCCEEEECCCCCCHHHH
KLLSVQYARENNIPYFGICLGMQIAIIEFAQNVIGIKEANSTEFDPQCKFPVIDLIKTKK
HHHHHEEHHCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCC
NDIKDTRQGKKNNKSNFGGTMRLGSQPCKLIFNSLSRKLYKKDTIIERHRHRYEVNNFLL
CHHHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKIEKNGLKIAGRSKKNNIVEIIEIFDHPWFIGCQFHPEFTSTPRDGHPLFIDFIKSAKK
HHHHHCCCEEECCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHC
NKKNNFKIKVKNV
CCCCCEEEEEECC
>Mature Secondary Structure 
TKNYIFITGGVVSSLGKGIAAASLGAVLEARNLKITIMKLDPYINVDPGTMSPIQHGEV
CCCEEEEECHHHHHHCCCHHHHHHHHHHEECCCEEEEEEECCEEECCCCCCCCCCCCEE
FVTEDGAETDLDLGHYERFIRTKMTCLNNFTTGSIYSEVLKKERRGDYLGSTIQVIPHIT
EEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHH
NAIKDRIILCSKNSDIILVEIGGTVGDIESLPFLEAIRQLAVDIGRKNVIYIHLTLVPYI
HHHHCEEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEHHH
KTAGEIKTKPTQHSVKELLSIGIQPDILICRSQKTVPINERKKIALFCNVPVNAVISLKD
HCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCEEEEEECCCCCEEEEECC
VDSIYTIPKLLKDQKLDNYICEYFKLNVPQADLKEWEKVIYEEKNASKEIIIGIIGKYIK
CHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEHHEEHHHHHC
LPDAYKSVIEALKHAGLKNKIKVKIELINSQEIENKNFKLLQNLNGILIPGGFGDRGIIG
CCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCHHHHHCCCCEEEECCCCCCHHHH
KLLSVQYARENNIPYFGICLGMQIAIIEFAQNVIGIKEANSTEFDPQCKFPVIDLIKTKK
HHHHHEEHHCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCC
NDIKDTRQGKKNNKSNFGGTMRLGSQPCKLIFNSLSRKLYKKDTIIERHRHRYEVNNFLL
CHHHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKIEKNGLKIAGRSKKNNIVEIIEIFDHPWFIGCQFHPEFTSTPRDGHPLFIDFIKSAKK
HHHHHCCCEEECCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHC
NKKNNFKIKVKNV
CCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438