Click here to switch to the map view.

The map label for this gene is eno

Identifier: 21672673

GI number: 21672673

Start: 453544

End: 454848

Strand: Direct

Name: eno

Synonym: BUsg400

Alternate gene names: 21672673

Gene position: 453544-454848 (Clockwise)

Preceding gene: 21672672

Following gene: 21672674

Centisome position: 70.71

GC content: 29.2

Gene sequence:

>1305_bases
ATGTCTAAAATATTAAAAATAATTGGTCGTGAAATCATCGATTCTAGAGGTAATCCTACCATAGAATGTGAAGTTTTGCT
TGAAGGAGGTTTTGTTGGTTTAGCTTCAGTACCTTCCGGAGCATCTACAGGTTCTTTTGAAACATGGGAATTAAGAGATC
AAGATAAAAATCGATTTATGGGTAAGGGTGTGCAAAAAGCAGTAGAAATAATAAATAATAAAATTTTCTACTCTTTAAAA
AATAAAAATGCAATAGATCAATTTGATATCGATCAAACCATGATTAATTTAGATGGTACAGAAAACAAATCTAATTTAGG
TTCCAATTCGATTTTGTCAGTATCTTTAGCAACTGCTAAAGCAGCTGCATCTTCTAAGGGCATGCCTTTATATCAACACA
TTGCAGAAATTAATAATACACCAGGCGTATTTTCCATGCCTTTGCCAATGATTAATATAATTAACGGTGGAAAACACGCG
AATAATAATATTGATATTCAAGAATTTATGATACAACCTATTTCAGCAAAATCCATTACAGAAGCTATACGTATAGGAGC
AGAAATATTTCATTCATTAGGAAATTTACTAAAAGATAAAGGAATGAGTACTACAGTAGGCGACGAGGGAGGATATGCTC
CAAATTTTAAATCTAATGAAGAAGCATTAAATGTAATTCAAGATGCAGTACACAAAACTAAATATAAGTTAGGAAAAGAT
ATTACATTAGCTATAGATTGCGCAGCTTCTGAGTTATACAATAAAACTAGAAAAAAATACCAATTCATCGGAGAAGGAAC
TGAATTTAGTTCACAAGAACTAACTCATTATTTAAAAAAATTGTCTAATAAATATCCTATTATTTCTATTGAAGATGGAC
AAGACGAATCAGATTGGGAAGGTTTTTTATATCAAACAAAAGAATTAGGTAATTCACTGCAATTAGTAGGAGATGATTTA
TTTGTTACTAATAAAAATATCTTAAAAAAAGGAATAAAAAAAGGTGTTGCAAATGCAATTTTAATAAAATTAAATCAAAT
TGGAACATTAACTGAAACAATTGAAACTATTAAAATAGCAAAAAAATTTAATTATGGTGTTATTATTTCTCATCGTTCAG
GTGAAACAGAAGATACATCAATAGCAGATTTATCAGTAGGAACAGCATCAGGGCAAATTAAAACAGGATCAATGAGTCGT
TCTGATAGAACTTCTAAATACAATCAATTAATCAGAATAGAAGAAATATTAAATAAAAAAAGAGCGCCTTTCTACGGACT
AAAAGAAGTAAAATCATCTTTTTAA

Upstream 100 bases:

>100_bases
GAATTTACTTCTACACCGCGCGATGGACATCCATTATTCATAGATTTTATTAAATCAGCAAAAAAAAATAAAAAAAATAA
TTTTAAAATAAAGGTAAAAA

Downstream 100 bases:

>100_bases
AGATAAGATATATTCTTAAAGAGAATCAGGTATTTTAAAATATATGCCTGTTTCTCATTAACATAAAATACTAATCATTT
TTTATGAGTCTAATTATATG

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MSKILKIIGREIIDSRGNPTIECEVLLEGGFVGLASVPSGASTGSFETWELRDQDKNRFMGKGVQKAVEIINNKIFYSLK
NKNAIDQFDIDQTMINLDGTENKSNLGSNSILSVSLATAKAAASSKGMPLYQHIAEINNTPGVFSMPLPMINIINGGKHA
NNNIDIQEFMIQPISAKSITEAIRIGAEIFHSLGNLLKDKGMSTTVGDEGGYAPNFKSNEEALNVIQDAVHKTKYKLGKD
ITLAIDCAASELYNKTRKKYQFIGEGTEFSSQELTHYLKKLSNKYPIISIEDGQDESDWEGFLYQTKELGNSLQLVGDDL
FVTNKNILKKGIKKGVANAILIKLNQIGTLTETIETIKIAKKFNYGVIISHRSGETEDTSIADLSVGTASGQIKTGSMSR
SDRTSKYNQLIRIEEILNKKRAPFYGLKEVKSSF

Sequences:

>Translated_434_residues
MSKILKIIGREIIDSRGNPTIECEVLLEGGFVGLASVPSGASTGSFETWELRDQDKNRFMGKGVQKAVEIINNKIFYSLK
NKNAIDQFDIDQTMINLDGTENKSNLGSNSILSVSLATAKAAASSKGMPLYQHIAEINNTPGVFSMPLPMINIINGGKHA
NNNIDIQEFMIQPISAKSITEAIRIGAEIFHSLGNLLKDKGMSTTVGDEGGYAPNFKSNEEALNVIQDAVHKTKYKLGKD
ITLAIDCAASELYNKTRKKYQFIGEGTEFSSQELTHYLKKLSNKYPIISIEDGQDESDWEGFLYQTKELGNSLQLVGDDL
FVTNKNILKKGIKKGVANAILIKLNQIGTLTETIETIKIAKKFNYGVIISHRSGETEDTSIADLSVGTASGQIKTGSMSR
SDRTSKYNQLIRIEEILNKKRAPFYGLKEVKSSF
>Mature_433_residues
SKILKIIGREIIDSRGNPTIECEVLLEGGFVGLASVPSGASTGSFETWELRDQDKNRFMGKGVQKAVEIINNKIFYSLKN
KNAIDQFDIDQTMINLDGTENKSNLGSNSILSVSLATAKAAASSKGMPLYQHIAEINNTPGVFSMPLPMINIINGGKHAN
NNIDIQEFMIQPISAKSITEAIRIGAEIFHSLGNLLKDKGMSTTVGDEGGYAPNFKSNEEALNVIQDAVHKTKYKLGKDI
TLAIDCAASELYNKTRKKYQFIGEGTEFSSQELTHYLKKLSNKYPIISIEDGQDESDWEGFLYQTKELGNSLQLVGDDLF
VTNKNILKKGIKKGVANAILIKLNQIGTLTETIETIKIAKKFNYGVIISHRSGETEDTSIADLSVGTASGQIKTGSMSRS
DRTSKYNQLIRIEEILNKKRAPFYGLKEVKSSF

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI301897477, Length=430, Percent_Identity=47.6744186046512, Blast_Score=383, Evalue=1e-106,
Organism=Homo sapiens, GI301897469, Length=430, Percent_Identity=47.6744186046512, Blast_Score=383, Evalue=1e-106,
Organism=Homo sapiens, GI4503571, Length=430, Percent_Identity=48.1395348837209, Blast_Score=383, Evalue=1e-106,
Organism=Homo sapiens, GI5803011, Length=430, Percent_Identity=45.8139534883721, Blast_Score=374, Evalue=1e-104,
Organism=Homo sapiens, GI301897479, Length=428, Percent_Identity=42.2897196261682, Blast_Score=327, Evalue=9e-90,
Organism=Homo sapiens, GI169201331, Length=339, Percent_Identity=27.4336283185841, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI169201757, Length=339, Percent_Identity=27.4336283185841, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI239744207, Length=339, Percent_Identity=27.4336283185841, Blast_Score=111, Evalue=1e-24,
Organism=Escherichia coli, GI1789141, Length=432, Percent_Identity=68.287037037037, Blast_Score=596, Evalue=1e-172,
Organism=Caenorhabditis elegans, GI71995829, Length=422, Percent_Identity=49.0521327014218, Blast_Score=378, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17536383, Length=422, Percent_Identity=49.0521327014218, Blast_Score=377, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI32563855, Length=181, Percent_Identity=45.3038674033149, Blast_Score=160, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6321693, Length=427, Percent_Identity=45.4332552693208, Blast_Score=345, Evalue=8e-96,
Organism=Saccharomyces cerevisiae, GI6324974, Length=423, Percent_Identity=46.3356973995272, Blast_Score=343, Evalue=3e-95,
Organism=Saccharomyces cerevisiae, GI6324969, Length=423, Percent_Identity=46.3356973995272, Blast_Score=343, Evalue=3e-95,
Organism=Saccharomyces cerevisiae, GI6323985, Length=423, Percent_Identity=46.0992907801418, Blast_Score=343, Evalue=4e-95,
Organism=Saccharomyces cerevisiae, GI6321968, Length=430, Percent_Identity=46.046511627907, Blast_Score=339, Evalue=4e-94,
Organism=Drosophila melanogaster, GI24580918, Length=420, Percent_Identity=47.8571428571429, Blast_Score=351, Evalue=5e-97,
Organism=Drosophila melanogaster, GI24580916, Length=420, Percent_Identity=47.8571428571429, Blast_Score=351, Evalue=5e-97,
Organism=Drosophila melanogaster, GI24580920, Length=420, Percent_Identity=47.8571428571429, Blast_Score=351, Evalue=5e-97,
Organism=Drosophila melanogaster, GI24580914, Length=420, Percent_Identity=47.8571428571429, Blast_Score=351, Evalue=5e-97,
Organism=Drosophila melanogaster, GI281360527, Length=420, Percent_Identity=47.8571428571429, Blast_Score=350, Evalue=1e-96,
Organism=Drosophila melanogaster, GI17137654, Length=420, Percent_Identity=47.8571428571429, Blast_Score=350, Evalue=1e-96,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_BUCAP (Q8K9E0)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660740.1
- ProteinModelPortal:   Q8K9E0
- SMR:   Q8K9E0
- EnsemblBacteria:   EBBUCT00000000040
- GeneID:   1005760
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg400
- GeneTree:   EBGT00050000007974
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- ProtClustDB:   PRK00077
- BioCyc:   BAPH198804:BUSG400-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 47739; Mature: 47608

Theoretical pI: Translated: 8.54; Mature: 8.54

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 209-209 ACT_SITE 343-343 BINDING 159-159 BINDING 168-168 BINDING 291-291 BINDING 318-318 BINDING 343-343 BINDING 394-394

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKILKIIGREIIDSRGNPTIECEVLLEGGFVGLASVPSGASTGSFETWELRDQDKNRFM
CHHHHHHHHHHHHCCCCCCCEEEEEEEECCEEEEECCCCCCCCCCEEEEECCCCHHHHHH
GKGVQKAVEIINNKIFYSLKNKNAIDQFDIDQTMINLDGTENKSNLGSNSILSVSLATAK
HHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEEHHHH
AAASSKGMPLYQHIAEINNTPGVFSMPLPMINIINGGKHANNNIDIQEFMIQPISAKSIT
HHHCCCCCHHHHHHHHHCCCCCEEECCCCEEEEECCCCCCCCCCCHHHHHHCCCCHHHHH
EAIRIGAEIFHSLGNLLKDKGMSTTVGDEGGYAPNFKSNEEALNVIQDAVHKTKYKLGKD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
ITLAIDCAASELYNKTRKKYQFIGEGTEFSSQELTHYLKKLSNKYPIISIEDGQDESDWE
EEEEEEHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHH
GFLYQTKELGNSLQLVGDDLFVTNKNILKKGIKKGVANAILIKLNQIGTLTETIETIKIA
HHHHHHHHHCCCEEEECCCEEEECHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHH
KKFNYGVIISHRSGETEDTSIADLSVGTASGQIKTGSMSRSDRTSKYNQLIRIEEILNKK
HHCCCCEEEECCCCCCCCCEEEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCC
RAPFYGLKEVKSSF
CCCCCCHHHHHHCC
>Mature Secondary Structure 
SKILKIIGREIIDSRGNPTIECEVLLEGGFVGLASVPSGASTGSFETWELRDQDKNRFM
HHHHHHHHHHHHCCCCCCCEEEEEEEECCEEEEECCCCCCCCCCEEEEECCCCHHHHHH
GKGVQKAVEIINNKIFYSLKNKNAIDQFDIDQTMINLDGTENKSNLGSNSILSVSLATAK
HHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEEHHHH
AAASSKGMPLYQHIAEINNTPGVFSMPLPMINIINGGKHANNNIDIQEFMIQPISAKSIT
HHHCCCCCHHHHHHHHHCCCCCEEECCCCEEEEECCCCCCCCCCCHHHHHHCCCCHHHHH
EAIRIGAEIFHSLGNLLKDKGMSTTVGDEGGYAPNFKSNEEALNVIQDAVHKTKYKLGKD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
ITLAIDCAASELYNKTRKKYQFIGEGTEFSSQELTHYLKKLSNKYPIISIEDGQDESDWE
EEEEEEHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHH
GFLYQTKELGNSLQLVGDDLFVTNKNILKKGIKKGVANAILIKLNQIGTLTETIETIKIA
HHHHHHHHHCCCEEEECCCEEEECHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHH
KKFNYGVIISHRSGETEDTSIADLSVGTASGQIKTGSMSRSDRTSKYNQLIRIEEILNKK
HHCCCCEEEECCCCCCCCCEEEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCC
RAPFYGLKEVKSSF
CCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438