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The map label for this gene is glnS [H]

Identifier: 21672671

GI number: 21672671

Start: 450031

End: 451734

Strand: Direct

Name: glnS [H]

Synonym: BUsg398

Alternate gene names: 21672671

Gene position: 450031-451734 (Clockwise)

Preceding gene: 21672668

Following gene: 21672672

Centisome position: 70.16

GC content: 23.83

Gene sequence:

>1704_bases
ATGAGATTTAAAAAAATGAACAATAAAATAGAAAAAAATGATAATAATTTTATTTATAAAATTATTAATGAAGATTTCAA
AAAAAATAAAAATTTGTTATTGCATACGCGTTTTCCTCCTGAACCTAATGGATATCTTCATATTGGACATGCTAAATCAA
TATGTTTAAATTTTGAACTTGCAAATGTATACAATGGATATTGTAATCTTCGTTTCGATGATACAAATCCAGTTAAGGAA
AACATCAAATATATAAACTCAATCAAAAACGATATTAAATGGTTGGGTTATCAATGGCATAAAAAAGTTCGTTATTCTTC
TGAATATTTTCCAATACTGTATGAATATGCTAAAGAATTAATTCAAAAAGGTTTAGCATACGTAGATGAATTAACAAAAG
AAGAAATACGTGAATACAGAGGCACTTTAAGTACCGTAGGAAAAAATAGTCCTTATAGAAATAGAAGTATTACAGAAAAC
TTAAAATTATTTGAAAAAATGAAAAAAGGAGAATTTAAAGAAGGAGAGGCATGCTTACGTGCAAAAATTAATATGAGTTC
TTCATTTATTGTTATGCGTGATCCTGTATTATATCGAATTATTTTCTCTACACATCACCAAACTAAAAAAAAATGGTGTA
TATATCCTACATATGATTTTGCTCATTGTTTATCTGATTCTATTGAAGGTATAACTCATTCTTTTTGTACATTAGAATTT
CAAGACAATAAAAATTTATATAATTGGATTTTAAAAAATACTAGTATCAAGCATTATTCAAAACAATATGAGTTCTCTAG
ATTAAATCTAGAATACTCAATTTTATCTAAAAGAAAAATTTCAATTTTAATTGAAAAAAAAATAGTTAACGGATGGGACG
ATCCAAGAATACTAACTATTTCTGGATTGAGAAAAAAAGGATATACAGCATCTTCTATTCGTGATTTTTGTCATAGAATT
GGTATCACTAAACAAAATAATTTAATAGAATTTTCTATGCTAGAATATTGTATTAGAAAAGAACTAAACAAAAAAGCAAT
ACGTACTATGGCTGTATTAGAACCAATTAAAATATTTCTATATAACATAGATAATGATCATGAAGAAAAATTAATAGTTC
CCAATCATCCAAAACATCTACATTTAGGGACTCACGAAATTATCTTTACAAATACAATATATATTGAACGAGAAGATTTC
AAAGAAAAATACGATAAAAAATATAAAAGATTAAAAATTGGAGAAGAAGTTCGTTTAAGATATGCATATATAATAAAAGC
AGAAAAAATAGAAAAAGATGAAAGTGGTAATATTATCAAAATAATATGCTTCTGTGATATTAACAGTTTAGGGAAAAAAC
CAATTAATAGAAAAAATCCTGCAGTAATACACTGGATTGCAGTAAAAAATTCATTTCCAGCTAAATTTAAATTATACAAT
CAATTATTTAAAATAAAAAATCCTGATCAAGAAAAAAATTTCTTATCTTATTTAAATTCTAATTCATTAATAGTAAAAAA
TGGCTTTGTCGAAAAAAAAATAGCTCAAAAAATAATAAAAAAAATGAGTAATAATTTTATAAAATTGTTTTTTCAATTTG
AAAGAACTGGCTACTTTTGTGTAGATGAAGTTGATTCAAAAGAAAATAAATTAGTTTTTAATCGTACTGTTAGTTTAAAA
GATTCATGGAATTTAAAAAAATGA

Upstream 100 bases:

>100_bases
TTTTAAAAAAATAATACTTTCTTTATTTTATAATATAAAAAATAAATAATCAATATAATTATTAATGTAAAATAAGTAAT
TTTTTATATAAAAAAATCAA

Downstream 100 bases:

>100_bases
AAAATTAAAATACATACTATACTTAATATCTTCAAATAAAATTTAAAAATTTTTATATTTGAAAATCTATTGAATTTATT
TTACTAAAATAATTTAATTA

Product: glutaminyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamine--tRNA ligase; GlnRS [H]

Number of amino acids: Translated: 567; Mature: 567

Protein sequence:

>567_residues
MRFKKMNNKIEKNDNNFIYKIINEDFKKNKNLLLHTRFPPEPNGYLHIGHAKSICLNFELANVYNGYCNLRFDDTNPVKE
NIKYINSIKNDIKWLGYQWHKKVRYSSEYFPILYEYAKELIQKGLAYVDELTKEEIREYRGTLSTVGKNSPYRNRSITEN
LKLFEKMKKGEFKEGEACLRAKINMSSSFIVMRDPVLYRIIFSTHHQTKKKWCIYPTYDFAHCLSDSIEGITHSFCTLEF
QDNKNLYNWILKNTSIKHYSKQYEFSRLNLEYSILSKRKISILIEKKIVNGWDDPRILTISGLRKKGYTASSIRDFCHRI
GITKQNNLIEFSMLEYCIRKELNKKAIRTMAVLEPIKIFLYNIDNDHEEKLIVPNHPKHLHLGTHEIIFTNTIYIEREDF
KEKYDKKYKRLKIGEEVRLRYAYIIKAEKIEKDESGNIIKIICFCDINSLGKKPINRKNPAVIHWIAVKNSFPAKFKLYN
QLFKIKNPDQEKNFLSYLNSNSLIVKNGFVEKKIAQKIIKKMSNNFIKLFFQFERTGYFCVDEVDSKENKLVFNRTVSLK
DSWNLKK

Sequences:

>Translated_567_residues
MRFKKMNNKIEKNDNNFIYKIINEDFKKNKNLLLHTRFPPEPNGYLHIGHAKSICLNFELANVYNGYCNLRFDDTNPVKE
NIKYINSIKNDIKWLGYQWHKKVRYSSEYFPILYEYAKELIQKGLAYVDELTKEEIREYRGTLSTVGKNSPYRNRSITEN
LKLFEKMKKGEFKEGEACLRAKINMSSSFIVMRDPVLYRIIFSTHHQTKKKWCIYPTYDFAHCLSDSIEGITHSFCTLEF
QDNKNLYNWILKNTSIKHYSKQYEFSRLNLEYSILSKRKISILIEKKIVNGWDDPRILTISGLRKKGYTASSIRDFCHRI
GITKQNNLIEFSMLEYCIRKELNKKAIRTMAVLEPIKIFLYNIDNDHEEKLIVPNHPKHLHLGTHEIIFTNTIYIEREDF
KEKYDKKYKRLKIGEEVRLRYAYIIKAEKIEKDESGNIIKIICFCDINSLGKKPINRKNPAVIHWIAVKNSFPAKFKLYN
QLFKIKNPDQEKNFLSYLNSNSLIVKNGFVEKKIAQKIIKKMSNNFIKLFFQFERTGYFCVDEVDSKENKLVFNRTVSLK
DSWNLKK
>Mature_567_residues
MRFKKMNNKIEKNDNNFIYKIINEDFKKNKNLLLHTRFPPEPNGYLHIGHAKSICLNFELANVYNGYCNLRFDDTNPVKE
NIKYINSIKNDIKWLGYQWHKKVRYSSEYFPILYEYAKELIQKGLAYVDELTKEEIREYRGTLSTVGKNSPYRNRSITEN
LKLFEKMKKGEFKEGEACLRAKINMSSSFIVMRDPVLYRIIFSTHHQTKKKWCIYPTYDFAHCLSDSIEGITHSFCTLEF
QDNKNLYNWILKNTSIKHYSKQYEFSRLNLEYSILSKRKISILIEKKIVNGWDDPRILTISGLRKKGYTASSIRDFCHRI
GITKQNNLIEFSMLEYCIRKELNKKAIRTMAVLEPIKIFLYNIDNDHEEKLIVPNHPKHLHLGTHEIIFTNTIYIEREDF
KEKYDKKYKRLKIGEEVRLRYAYIIKAEKIEKDESGNIIKIICFCDINSLGKKPINRKNPAVIHWIAVKNSFPAKFKLYN
QLFKIKNPDQEKNFLSYLNSNSLIVKNGFVEKKIAQKIIKKMSNNFIKLFFQFERTGYFCVDEVDSKENKLVFNRTVSLK
DSWNLKK

Specific function: Unknown

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI4826960, Length=534, Percent_Identity=44.1947565543071, Blast_Score=435, Evalue=1e-122,
Organism=Homo sapiens, GI62241042, Length=510, Percent_Identity=31.9607843137255, Blast_Score=244, Evalue=1e-64,
Organism=Escherichia coli, GI1786895, Length=557, Percent_Identity=57.8096947935368, Blast_Score=711, Evalue=0.0,
Organism=Escherichia coli, GI1788743, Length=201, Percent_Identity=24.3781094527363, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17543332, Length=536, Percent_Identity=42.3507462686567, Blast_Score=399, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17511007, Length=519, Percent_Identity=29.6724470134875, Blast_Score=221, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6324742, Length=561, Percent_Identity=38.8591800356506, Blast_Score=377, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6321192, Length=522, Percent_Identity=32.7586206896552, Blast_Score=281, Evalue=3e-76,
Organism=Drosophila melanogaster, GI17864488, Length=535, Percent_Identity=42.9906542056075, Blast_Score=431, Evalue=1e-121,
Organism=Drosophila melanogaster, GI24649466, Length=449, Percent_Identity=33.630289532294, Blast_Score=236, Evalue=4e-62,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR004514
- InterPro:   IPR022861
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR020059
- InterPro:   IPR020056
- InterPro:   IPR011035
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c; PF03950 tRNA-synt_1c_C [H]

EC number: =6.1.1.18 [H]

Molecular weight: Translated: 67461; Mature: 67461

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFKKMNNKIEKNDNNFIYKIINEDFKKNKNLLLHTRFPPEPNGYLHIGHAKSICLNFEL
CCCHHHCCCCCCCCCCEEEEEECHHHCCCCCEEEEECCCCCCCCEEEECCCCEEEEEEEE
ANVYNGYCNLRFDDTNPVKENIKYINSIKNDIKWLGYQWHKKVRYSSEYFPILYEYAKEL
HHEECCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHH
IQKGLAYVDELTKEEIREYRGTLSTVGKNSPYRNRSITENLKLFEKMKKGEFKEGEACLR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEE
AKINMSSSFIVMRDPVLYRIIFSTHHQTKKKWCIYPTYDFAHCLSDSIEGITHSFCTLEF
EEECCCCCEEEEECCEEEEEEHHHCCCCCCCEEEECCHHHHHHHHHHHHHCEEEEEEEEE
QDNKNLYNWILKNTSIKHYSKQYEFSRLNLEYSILSKRKISILIEKKIVNGWDDPRILTI
CCCCCHHHHHHCCCCHHHHHHHCCCEEEEEEEEECCCCEEEEEEEEHHHCCCCCCEEEEE
SGLRKKGYTASSIRDFCHRIGITKQNNLIEFSMLEYCIRKELNKKAIRTMAVLEPIKIFL
ECCCCCCCCHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEE
YNIDNDHEEKLIVPNHPKHLHLGTHEIIFTNTIYIEREDFKEKYDKKYKRLKIGEEVRLR
EECCCCCCCEEECCCCCCEEECCCEEEEEEEEEEEEHHHHHHHHHHHHHHCCCCHHHEEE
YAYIIKAEKIEKDESGNIIKIICFCDINSLGKKPINRKNPAVIHWIAVKNSFPAKFKLYN
EEEEEEHHHCCCCCCCCEEEEEEEECCCHHCCCCCCCCCCEEEEEEEECCCCCHHHHHHH
QLFKIKNPDQEKNFLSYLNSNSLIVKNGFVEKKIAQKIIKKMSNNFIKLFFQFERTGYFC
HHHHCCCCCHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCEEEEEEEEECCCEEE
VDEVDSKENKLVFNRTVSLKDSWNLKK
EECCCCCCCEEEEEEEEEECCCCCCCC
>Mature Secondary Structure
MRFKKMNNKIEKNDNNFIYKIINEDFKKNKNLLLHTRFPPEPNGYLHIGHAKSICLNFEL
CCCHHHCCCCCCCCCCEEEEEECHHHCCCCCEEEEECCCCCCCCEEEECCCCEEEEEEEE
ANVYNGYCNLRFDDTNPVKENIKYINSIKNDIKWLGYQWHKKVRYSSEYFPILYEYAKEL
HHEECCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHH
IQKGLAYVDELTKEEIREYRGTLSTVGKNSPYRNRSITENLKLFEKMKKGEFKEGEACLR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEE
AKINMSSSFIVMRDPVLYRIIFSTHHQTKKKWCIYPTYDFAHCLSDSIEGITHSFCTLEF
EEECCCCCEEEEECCEEEEEEHHHCCCCCCCEEEECCHHHHHHHHHHHHHCEEEEEEEEE
QDNKNLYNWILKNTSIKHYSKQYEFSRLNLEYSILSKRKISILIEKKIVNGWDDPRILTI
CCCCCHHHHHHCCCCHHHHHHHCCCEEEEEEEEECCCCEEEEEEEEHHHCCCCCCEEEEE
SGLRKKGYTASSIRDFCHRIGITKQNNLIEFSMLEYCIRKELNKKAIRTMAVLEPIKIFL
ECCCCCCCCHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEE
YNIDNDHEEKLIVPNHPKHLHLGTHEIIFTNTIYIEREDFKEKYDKKYKRLKIGEEVRLR
EECCCCCCCEEECCCCCCEEECCCEEEEEEEEEEEEHHHHHHHHHHHHHHCCCCHHHEEE
YAYIIKAEKIEKDESGNIIKIICFCDINSLGKKPINRKNPAVIHWIAVKNSFPAKFKLYN
EEEEEEHHHCCCCCCCCEEEEEEEECCCHHCCCCCCCCCCEEEEEEEECCCCCHHHHHHH
QLFKIKNPDQEKNFLSYLNSNSLIVKNGFVEKKIAQKIIKKMSNNFIKLFFQFERTGYFC
HHHHCCCCCHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCEEEEEEEEECCCEEE
VDEVDSKENKLVFNRTVSLKDSWNLKK
EECCCCCCCEEEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438 [H]