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The map label for this gene is yggJ

Identifier: 21672668

GI number: 21672668

Start: 447383

End: 448123

Strand: Direct

Name: yggJ

Synonym: BUsg395

Alternate gene names: 21672668

Gene position: 447383-448123 (Clockwise)

Preceding gene: 21672666

Following gene: 21672671

Centisome position: 69.75

GC content: 22.81

Gene sequence:

>741_bases
ATGAAAAAACGTATCCCAAGAATTTATATCAAATATTTTTTACAAATTAATCAAGTAATATCATTATCGAAATCTTATAC
GCATTACATAAAAAAAGTACTACGGATGAAAAAAAAAGATAAATTAGAAATATTTAATAATACTAATTATATTTTTTTTT
CTGAAATATTAGAAATAAATAATCACACAATAGAAATAATTATTTTAAAAAAACAAATAAAAAATCTTGAATCACCGCTG
TTAATTCATTTAGGACAAGTGATATCAAAAAATGAAAAAATGAATTTTTCTATCCAAAAATCTGTTGAGCTGGGAGTAAA
TACTATTACACCATTATTTTCAGAATATTGTAACTTTCAAAATAAATTAATATGTTTTCCAAAAAAAAAAATACATTGGG
AAAATATAGTAATTTCTTCTTGTGAACAATGTCATCGTAATAATATTCCTGAAATTAAAAATCCAGAATATATACTTCCA
TGGTGTAAAAGAACATATGAAAATGAAACAAAAATAGTTTTTAGTCCAAGTGCTACTTTCACTATTAATGAATTGCCTGA
AAAAATCAATTGTGTTCGATTATTAATCGGTAGTGAAGGTGGATTTTCATTTTTAGAAATAGAAAAAATAATTCAATATG
GATTTATTCCTATTAAATTAGGACCTAGAATTTTAAGAACAGAAACAGCTGTAATTGCAGCAATTACTGCTTTACAAATA
AAATTTGGTGATTTAAGTTAA

Upstream 100 bases:

>100_bases
ATGCGAAAGAGAAAATTTAATCTTTCAAAAACAGGGAAATCATAATAATTACATTTATAAAAAATGTAAAATTTATTATT
CAAAAATAAAAAATTAAAAA

Downstream 100 bases:

>100_bases
TTTTGATGAAAAAAGTGTATTTTATTAGGTGTTAAAACAATAGGTAGGGAAATATCCCAAGGCTGTCTAGGTATGTAATT
TACGAACTGAAAATCATAAG

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MKKRIPRIYIKYFLQINQVISLSKSYTHYIKKVLRMKKKDKLEIFNNTNYIFFSEILEINNHTIEIIILKKQIKNLESPL
LIHLGQVISKNEKMNFSIQKSVELGVNTITPLFSEYCNFQNKLICFPKKKIHWENIVISSCEQCHRNNIPEIKNPEYILP
WCKRTYENETKIVFSPSATFTINELPEKINCVRLLIGSEGGFSFLEIEKIIQYGFIPIKLGPRILRTETAVIAAITALQI
KFGDLS

Sequences:

>Translated_246_residues
MKKRIPRIYIKYFLQINQVISLSKSYTHYIKKVLRMKKKDKLEIFNNTNYIFFSEILEINNHTIEIIILKKQIKNLESPL
LIHLGQVISKNEKMNFSIQKSVELGVNTITPLFSEYCNFQNKLICFPKKKIHWENIVISSCEQCHRNNIPEIKNPEYILP
WCKRTYENETKIVFSPSATFTINELPEKINCVRLLIGSEGGFSFLEIEKIIQYGFIPIKLGPRILRTETAVIAAITALQI
KFGDLS
>Mature_246_residues
MKKRIPRIYIKYFLQINQVISLSKSYTHYIKKVLRMKKKDKLEIFNNTNYIFFSEILEINNHTIEIIILKKQIKNLESPL
LIHLGQVISKNEKMNFSIQKSVELGVNTITPLFSEYCNFQNKLICFPKKKIHWENIVISSCEQCHRNNIPEIKNPEYILP
WCKRTYENETKIVFSPSATFTINELPEKINCVRLLIGSEGGFSFLEIEKIIQYGFIPIKLGPRILRTETAVIAAITALQI
KFGDLS

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family

Homologues:

Organism=Escherichia coli, GI87082186, Length=243, Percent_Identity=46.5020576131687, Blast_Score=225, Evalue=2e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSME_BUCAP (Q8K9E4)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660735.1
- ProteinModelPortal:   Q8K9E4
- SMR:   Q8K9E4
- EnsemblBacteria:   EBBUCT00000000290
- GeneID:   1005763
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg395
- GeneTree:   EBGT00050000008116
- HOGENOM:   HBG664092
- OMA:   WCRVLRA
- ProtClustDB:   PRK11713
- BioCyc:   BAPH198804:BUSG395-MONOMER
- GO:   GO:0005737
- InterPro:   IPR015947
- InterPro:   IPR006700
- TIGRFAMs:   TIGR00046

Pfam domain/function: PF04452 Methyltrans_RNA; SSF88697 PUA-like

EC number: =2.1.1.193

Molecular weight: Translated: 28743; Mature: 28743

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRIPRIYIKYFLQINQVISLSKSYTHYIKKVLRMKKKDKLEIFNNTNYIFFSEILEIN
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEEHHHHCC
NHTIEIIILKKQIKNLESPLLIHLGQVISKNEKMNFSIQKSVELGVNTITPLFSEYCNFQ
CCEEEEEEEEHHHCCCCCHHHHHHHHHHCCCCCCCEEEEHHHHCCCHHHHHHHHHHCCCC
NKLICFPKKKIHWENIVISSCEQCHRNNIPEIKNPEYILPWCKRTYENETKIVFSPSATF
CCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHCCCCCCEEEECCCCEE
TINELPEKINCVRLLIGSEGGFSFLEIEKIIQYGFIPIKLGPRILRTETAVIAAITALQI
EHHHCCCHHCEEEEEEECCCCCHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHEEE
KFGDLS
EECCCC
>Mature Secondary Structure
MKKRIPRIYIKYFLQINQVISLSKSYTHYIKKVLRMKKKDKLEIFNNTNYIFFSEILEIN
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEEHHHHCC
NHTIEIIILKKQIKNLESPLLIHLGQVISKNEKMNFSIQKSVELGVNTITPLFSEYCNFQ
CCEEEEEEEEHHHCCCCCHHHHHHHHHHCCCCCCCEEEEHHHHCCCHHHHHHHHHHCCCC
NKLICFPKKKIHWENIVISSCEQCHRNNIPEIKNPEYILPWCKRTYENETKIVFSPSATF
CCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHCCCCCCEEEECCCCEE
TINELPEKINCVRLLIGSEGGFSFLEIEKIIQYGFIPIKLGPRILRTETAVIAAITALQI
EHHHCCCHHCEEEEEEECCCCCHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHEEE
KFGDLS
EECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438