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The map label for this gene is gshA

Identifier: 21672666

GI number: 21672666

Start: 443649

End: 445205

Strand: Direct

Name: gshA

Synonym: BUsg392

Alternate gene names: 21672666

Gene position: 443649-445205 (Clockwise)

Preceding gene: 21672665

Following gene: 21672668

Centisome position: 69.16

GC content: 23.7

Gene sequence:

>1557_bases
TTGATAGAGGATATCTCAAAAAAAATTTCTTGGCTAGAAAAAAATCCTGAAATAATAAAAAATATTTTTCGAGGTATTGA
ACGCGAAACTTTAAGAATTAAAAAAAATGGAAAATTTTCTAATACTAAACATCCTTATTTGCTTGGCTCGCCTTTAACAC
ATAGATGGATTACAACTGATTTTTCAGAAAATTTATTAGAATTTATCACACCTACTAGTAGTAACATAGATTATTTAATA
AAATTTTTAAAAAATTTACATTCTTTCGTCTCATATAAAATAAAACATGAACGAATGTGGCCATTTAGTATTCCATATCT
TTATAATAAATCAACAGCTATTAAAATAGCTGAATACGGAACCTCTCATCTTGGAAAAATAAAAAATATCTATAGAAAAG
GTTTAAAAAATCGATACGGTGATTTAGTAAATACTATCTCAGGAATACACTATAATTTTTCTTTACCTAAAATATTTTGG
AAGAATTGGAAAAAGTCTGAAATAAATCTAAAAAATGAAGATTATATTTCGTCTGGTTACTTAAATTTAATTCGTAATTA
TTATCGATTTGGATGGGTTATTACTTATTTATTTGGAGCATCACCTGCAATATCAAAATATTTTTTAAAAGATAAAAAAT
ACAAATTTAAAAAAAATATAGAAAATATACTATATTTACCGTGGTCAACTTCATTAAGATTAAGTGATATTGGATATACT
AAAACACCTACTATAAAACTTAATATTATGTTTAATGATTTAAAATCATATATTAAATCTTTACGAAAAGCAATAAATAC
ACCTTCAAAAAAATTTTCTAAAATAGGAATAAAAGATAAACAAGGCGAATTTCTACAATTAAATACAAATATATTACAAA
TGGAAAGTGAACTTTATACCCCAATAAGACCAAAAAGACAAACTAATCCTGGCGAATCACTTTTAGAAGCTCTCGGAAAA
AGAGGTATTGAATATATAGAAATACGTTCCTTAGATATAAATCCATTCTCCTCAATAGGAATAAATAAAAAACAAATACT
TATACTAGATTTATTTCTAATCTGGTGTGCACTGATTCATGCACCTCAGATGAATAAAGAAAACTTTTTAGTAAATAATA
AAAATTGGAACAAAATTATTTTTGAAGGAAGAAAACCTCATCAAAAAATTTATATCAATAGTCAATATGAAACAAAAACA
TTAATTGAAATTGGGCAAATAATTTTTCAAGATTTAAAAAAAATTGCTAAAATACTTGATTATCAATCTAAAAATTTTGA
ATATCAAAAAGCATGTCAAGAAACGATACTTTTTTTTAAAAATCCAGAGTTAACTTATTCAGCAAAATTTCTACAACTTC
TAATAAAACATGGCATAAAAAAAACTGGATTAAATTTAGCAAATAAATATCACAAAGAATTTATTAATAGATATAATTAT
AATTCAGAAAAAAACATCTTAGAACGGGAAACTATACTTTCGCATAAGAAGCAAAAACAGATAGAAAATAATGAAATTTC
ATTTAAAAAAAATAATTTCTCCCATGCTAACAATTAA

Upstream 100 bases:

>100_bases
CAAAAATGTCCTAATCAAGCAGATACCATAAAAAATAAATCCTTAAAAATTAATACATTTAAAAAAAGATAATCTTTTTA
AAAAAATTAAGGAGTAAAAA

Downstream 100 bases:

>100_bases
CATGGGAAATAATTAAAAATCAACATAATTTTCCGTATTAAGTTCATTTTACTATACTAGTAAATACATATAATATATTT
AATTTATTTGTTTGCTATAA

Product: glutamate--cysteine ligase

Products: NA

Alternate protein names: Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase

Number of amino acids: Translated: 518; Mature: 518

Protein sequence:

>518_residues
MIEDISKKISWLEKNPEIIKNIFRGIERETLRIKKNGKFSNTKHPYLLGSPLTHRWITTDFSENLLEFITPTSSNIDYLI
KFLKNLHSFVSYKIKHERMWPFSIPYLYNKSTAIKIAEYGTSHLGKIKNIYRKGLKNRYGDLVNTISGIHYNFSLPKIFW
KNWKKSEINLKNEDYISSGYLNLIRNYYRFGWVITYLFGASPAISKYFLKDKKYKFKKNIENILYLPWSTSLRLSDIGYT
KTPTIKLNIMFNDLKSYIKSLRKAINTPSKKFSKIGIKDKQGEFLQLNTNILQMESELYTPIRPKRQTNPGESLLEALGK
RGIEYIEIRSLDINPFSSIGINKKQILILDLFLIWCALIHAPQMNKENFLVNNKNWNKIIFEGRKPHQKIYINSQYETKT
LIEIGQIIFQDLKKIAKILDYQSKNFEYQKACQETILFFKNPELTYSAKFLQLLIKHGIKKTGLNLANKYHKEFINRYNY
NSEKNILERETILSHKKQKQIENNEISFKKNNFSHANN

Sequences:

>Translated_518_residues
MIEDISKKISWLEKNPEIIKNIFRGIERETLRIKKNGKFSNTKHPYLLGSPLTHRWITTDFSENLLEFITPTSSNIDYLI
KFLKNLHSFVSYKIKHERMWPFSIPYLYNKSTAIKIAEYGTSHLGKIKNIYRKGLKNRYGDLVNTISGIHYNFSLPKIFW
KNWKKSEINLKNEDYISSGYLNLIRNYYRFGWVITYLFGASPAISKYFLKDKKYKFKKNIENILYLPWSTSLRLSDIGYT
KTPTIKLNIMFNDLKSYIKSLRKAINTPSKKFSKIGIKDKQGEFLQLNTNILQMESELYTPIRPKRQTNPGESLLEALGK
RGIEYIEIRSLDINPFSSIGINKKQILILDLFLIWCALIHAPQMNKENFLVNNKNWNKIIFEGRKPHQKIYINSQYETKT
LIEIGQIIFQDLKKIAKILDYQSKNFEYQKACQETILFFKNPELTYSAKFLQLLIKHGIKKTGLNLANKYHKEFINRYNY
NSEKNILERETILSHKKQKQIENNEISFKKNNFSHANN
>Mature_518_residues
MIEDISKKISWLEKNPEIIKNIFRGIERETLRIKKNGKFSNTKHPYLLGSPLTHRWITTDFSENLLEFITPTSSNIDYLI
KFLKNLHSFVSYKIKHERMWPFSIPYLYNKSTAIKIAEYGTSHLGKIKNIYRKGLKNRYGDLVNTISGIHYNFSLPKIFW
KNWKKSEINLKNEDYISSGYLNLIRNYYRFGWVITYLFGASPAISKYFLKDKKYKFKKNIENILYLPWSTSLRLSDIGYT
KTPTIKLNIMFNDLKSYIKSLRKAINTPSKKFSKIGIKDKQGEFLQLNTNILQMESELYTPIRPKRQTNPGESLLEALGK
RGIEYIEIRSLDINPFSSIGINKKQILILDLFLIWCALIHAPQMNKENFLVNNKNWNKIIFEGRKPHQKIYINSQYETKT
LIEIGQIIFQDLKKIAKILDYQSKNFEYQKACQETILFFKNPELTYSAKFLQLLIKHGIKKTGLNLANKYHKEFINRYNY
NSEKNILERETILSHKKQKQIENNEISFKKNNFSHANN

Specific function: Glutathione biosynthesis; first step. [C]

COG id: COG2918

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1789044, Length=514, Percent_Identity=47.2762645914397, Blast_Score=518, Evalue=1e-148,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSH1_BUCAP (P58994)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660733.1
- ProteinModelPortal:   P58994
- SMR:   P58994
- EnsemblBacteria:   EBBUCT00000000171
- GeneID:   1005592
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg392
- GeneTree:   EBGT00050000007855
- HOGENOM:   HBG289023
- OMA:   FGLIRNY
- ProtClustDB:   PRK02107
- BioCyc:   BAPH198804:BUSG392-MONOMER
- HAMAP:   MF_00578
- InterPro:   IPR007370
- InterPro:   IPR006334
- TIGRFAMs:   TIGR01434

Pfam domain/function: PF04262 Glu_cys_ligase

EC number: =6.3.2.2

Molecular weight: Translated: 61251; Mature: 61251

Theoretical pI: Translated: 10.38; Mature: 10.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEDISKKISWLEKNPEIIKNIFRGIERETLRIKKNGKFSNTKHPYLLGSPLTHRWITTD
CCCHHHHHHHHHHCCHHHHHHHHHCCCHHEEEEECCCCCCCCCCCEEECCCCCCCEEECC
FSENLLEFITPTSSNIDYLIKFLKNLHSFVSYKIKHERMWPFSIPYLYNKSTAIKIAEYG
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEECCEEECCCCEEEEECCC
TSHLGKIKNIYRKGLKNRYGDLVNTISGIHYNFSLPKIFWKNWKKSEINLKNEDYISSGY
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCHHHHHCCCCCCCCCCCCCCHHHHHH
LNLIRNYYRFGWVITYLFGASPAISKYFLKDKKYKFKKNIENILYLPWSTSLRLSDIGYT
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHCCCCCEEEECCCCCEEEECCCCC
KTPTIKLNIMFNDLKSYIKSLRKAINTPSKKFSKIGIKDKQGEFLQLNTNILQMESELYT
CCCEEEEEEEHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCEEEECCHHHHHHHHHCC
PIRPKRQTNPGESLLEALGKRGIEYIEIRSLDINPFSSIGINKKQILILDLFLIWCALIH
CCCCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCHHCCCCCCHHHHHHHHHHHHHHHC
APQMNKENFLVNNKNWNKIIFEGRKPHQKIYINSQYETKTLIEIGQIIFQDLKKIAKILD
CCCCCCCCEEEECCCCCEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHC
YQSKNFEYQKACQETILFFKNPELTYSAKFLQLLIKHGIKKTGLNLANKYHKEFINRYNY
CCCCCCHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHCCHHHCHHHHHHHHHHHHHHCCC
NSEKNILERETILSHKKQKQIENNEISFKKNNFSHANN
CCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCC
>Mature Secondary Structure
MIEDISKKISWLEKNPEIIKNIFRGIERETLRIKKNGKFSNTKHPYLLGSPLTHRWITTD
CCCHHHHHHHHHHCCHHHHHHHHHCCCHHEEEEECCCCCCCCCCCEEECCCCCCCEEECC
FSENLLEFITPTSSNIDYLIKFLKNLHSFVSYKIKHERMWPFSIPYLYNKSTAIKIAEYG
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEECCEEECCCCEEEEECCC
TSHLGKIKNIYRKGLKNRYGDLVNTISGIHYNFSLPKIFWKNWKKSEINLKNEDYISSGY
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCHHHHHCCCCCCCCCCCCCCHHHHHH
LNLIRNYYRFGWVITYLFGASPAISKYFLKDKKYKFKKNIENILYLPWSTSLRLSDIGYT
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHCCCCCEEEECCCCCEEEECCCCC
KTPTIKLNIMFNDLKSYIKSLRKAINTPSKKFSKIGIKDKQGEFLQLNTNILQMESELYT
CCCEEEEEEEHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCEEEECCHHHHHHHHHCC
PIRPKRQTNPGESLLEALGKRGIEYIEIRSLDINPFSSIGINKKQILILDLFLIWCALIH
CCCCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCHHCCCCCCHHHHHHHHHHHHHHHC
APQMNKENFLVNNKNWNKIIFEGRKPHQKIYINSQYETKTLIEIGQIIFQDLKKIAKILD
CCCCCCCCEEEECCCCCEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHC
YQSKNFEYQKACQETILFFKNPELTYSAKFLQLLIKHGIKKTGLNLANKYHKEFINRYNY
CCCCCCHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHCCHHHCHHHHHHHHHHHHHHCCC
NSEKNILERETILSHKKQKQIENNEISFKKNNFSHANN
CCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438