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The map label for this gene is rnhA

Identifier: 21672520

GI number: 21672520

Start: 278071

End: 278556

Strand: Reverse

Name: rnhA

Synonym: BUsg239

Alternate gene names: 21672520

Gene position: 278556-278071 (Counterclockwise)

Preceding gene: 21672525

Following gene: 21672519

Centisome position: 43.43

GC content: 26.54

Gene sequence:

>486_bases
ATGTTAAAATTAGTTAAAATGTTTTCAGATGGTTCTTGTTTAGGAAATCCTGGATCAGGTGGATATGGTACAATATTACG
TTATAAATTACATGAAAAGATATTAACTTCCGGTTTTTTTTTAACTACAAATAATAGAATGGAATTAATGGGTGTAATAT
GTGGTTTAGAATCTCTTAAAGAATCTTGCATAGTTGAAATTACAATTGACAGTCAATATGTAAAGCAAGGTATTACAAAT
TGGATTGCTACATGGGAGAAAAAGAAATGGAAAACAACTAAAAAAAAATTAATAAAAAATTTAGATTTATGGTTACGTAT
CAATGCTGTTATAAAAAATCATCATATTACATGGTTTTGGGTTAAAGCCCATATGGGTCATTTAGAAAACGAAAGATGTG
ATAAAATAGCACGTCAATCTGCTCAAAGTCCTTCAGTTAAAGATTTTTTTTATGAAAATAACTTTTATCAAAACAAAAAT
TTATAA

Upstream 100 bases:

>100_bases
ATTCATTCCTGTTGTTTCAGTATCTAAAACAATTATTCTTTGTGTATTATTCATCATAAATGATCTATTTAAGTTATTTA
ATATTGGAATAATTATTAAA

Downstream 100 bases:

>100_bases
AATTTTTTTTAATGGAATTAAACATGATTTTAACAAGTATTTCTGTATTAAAAGATAATTACGTTTGGATTCTATACAAC
GATAATTGTTCTTGTATAAT

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MLKLVKMFSDGSCLGNPGSGGYGTILRYKLHEKILTSGFFLTTNNRMELMGVICGLESLKESCIVEITIDSQYVKQGITN
WIATWEKKKWKTTKKKLIKNLDLWLRINAVIKNHHITWFWVKAHMGHLENERCDKIARQSAQSPSVKDFFYENNFYQNKN
L

Sequences:

>Translated_161_residues
MLKLVKMFSDGSCLGNPGSGGYGTILRYKLHEKILTSGFFLTTNNRMELMGVICGLESLKESCIVEITIDSQYVKQGITN
WIATWEKKKWKTTKKKLIKNLDLWLRINAVIKNHHITWFWVKAHMGHLENERCDKIARQSAQSPSVKDFFYENNFYQNKN
L
>Mature_161_residues
MLKLVKMFSDGSCLGNPGSGGYGTILRYKLHEKILTSGFFLTTNNRMELMGVICGLESLKESCIVEITIDSQYVKQGITN
WIATWEKKKWKTTKKKLIKNLDLWLRINAVIKNHHITWFWVKAHMGHLENERCDKIARQSAQSPSVKDFFYENNFYQNKN
L

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain

Homologues:

Organism=Homo sapiens, GI21359816, Length=147, Percent_Identity=29.2517006802721, Blast_Score=68, Evalue=5e-12,
Organism=Escherichia coli, GI1786408, Length=152, Percent_Identity=57.8947368421053, Blast_Score=201, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI115532542, Length=141, Percent_Identity=27.6595744680851, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17137476, Length=146, Percent_Identity=32.1917808219178, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI45552509, Length=146, Percent_Identity=32.1917808219178, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH_BUCAP (Q08885)

Other databases:

- EMBL:   L18927
- EMBL:   AE013218
- PIR:   I40593
- RefSeq:   NP_660587.1
- ProteinModelPortal:   Q08885
- SMR:   Q08885
- EnsemblBacteria:   EBBUCT00000000497
- GeneID:   1005440
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg239
- GeneTree:   EBGT00050000008216
- HOGENOM:   HBG742437
- OMA:   NGWRTAD
- BioCyc:   BAPH198804:BUSG239-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00042
- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156

Pfam domain/function: PF00075 RnaseH; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 18734; Mature: 18734

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKLVKMFSDGSCLGNPGSGGYGTILRYKLHEKILTSGFFLTTNNRMELMGVICGLESLK
CCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHC
ESCIVEITIDSQYVKQGITNWIATWEKKKWKTTKKKLIKNLDLWLRINAVIKNHHITWFW
CCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCEEEEE
VKAHMGHLENERCDKIARQSAQSPSVKDFFYENNFYQNKNL
EEEHHCCCCHHHHHHHHHHCCCCCCHHHHHCCCCEECCCCC
>Mature Secondary Structure
MLKLVKMFSDGSCLGNPGSGGYGTILRYKLHEKILTSGFFLTTNNRMELMGVICGLESLK
CCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHC
ESCIVEITIDSQYVKQGITNWIATWEKKKWKTTKKKLIKNLDLWLRINAVIKNHHITWFW
CCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCEEEEE
VKAHMGHLENERCDKIARQSAQSPSVKDFFYENNFYQNKNL
EEEHHCCCCHHHHHHHHHHCCCCCCHHHHHCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7507875; 12089438