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The map label for this gene is ilvI

Identifier: 21672502

GI number: 21672502

Start: 249790

End: 251505

Strand: Reverse

Name: ilvI

Synonym: BUsg220

Alternate gene names: 21672502

Gene position: 251505-249790 (Counterclockwise)

Preceding gene: 21672504

Following gene: 21672501

Centisome position: 39.21

GC content: 31.64

Gene sequence:

>1716_bases
TTGGAAATATTATCAGGAGCCGAAATGGTCATCAGATCATTAATTAATCAGGGAATACAGCATATATTTGGTTATCCTGG
TGGTGCTGTCCTAGATATTTATGATGCTCTAAAAACTGTTGGCGGAGTTGAACATATTTTAGTAAGACATGAACAAGCAG
CAACTCACATGGCTGATGGATATGCGAGATCTACTGGGAAAATAGGTGTTGTATTAGTCACATCAGGACCAGGTGCTACC
AACGCTATTACCGGTATTGCTACAGCTTATATGGATTCTATCCCAATGGTCGTCATTTCCGGTCAAGTTGCCTCATCATT
AATCGGATACGATGCATTTCAAGAATGTGACATGATTGGTATTTCTCGTCCTATAGTTAAACATAGTTTTTTAGTAAAAA
GAACTGAAGATATACCTATAATTTTTAAGAAAGCTTTTTGGTTAGCATCTACCGGACGACCTGGTCCAGTAGTTATTGAT
TTACCAAAAGATATTTTAAAAAAAACAAATAAATACAATTTTATATGGCCAAAAAATATACATATACGTTCCTATAACCC
AACAACTAAAGGTCATCAGGGACAAATCAAAAAAGCATTACGTATTCTATTAAAAGCAAAAAAACCTATTATCTACGCAG
GTGGAGGAATAATCAGTTCTAACAGCAGTGAAGAATTACGTATATTTGCAGAAAAGATCAATTGTCCTGTAACAACATCT
TTAATGGGATTAGGAGCATTTCCTGGAAATCACATTCAAAGTATTTCTATGTTAGGTATGCATGGAACTTATGAAGCTAA
TATGGCTATGCATTATTCTGATGTAATTTTTGCAATTGGTGTACGATTTGATGATCGAACAACAAATAATTTAAAGAAAT
ATTGTCCAAATGCTACTATTTTACATGTTGACATTGATCCTACTTCAATTTCTAAAACTGTTTCAGCAGATATTCCTATT
GTTGGAGATGCTAAACAAGTTTTAAAAGAAATGATTGAATTAATAAAAAAAGAAAAACAAATTCATTCTTTAAAAGAATG
GTGGTCTTCCATAGGAAAATGGAAAAAAATTAAAAGCTTAGAATACAATAAAAAAAGTAATAAAATAAAACCACAAAAAA
TTATTCAAACTCTTTTTAAGTTAACAAAGGGAACGTCTTATATTACTTCAGATGTTGGTCAACATCAAATGTTTACTGCG
TTATATTATCAATTTAATAAACCTAGACGTTGGATTAATTCAGGTGGATTAGGTACTATGGGATTTGGATTGCCTGCTGC
TTTAGGGGTCAAATTAGCATTACCGAAAGCTACTGTAATTTGTGTTACTGGAGATGGCAGTATTCAAATGAATATTCAAG
AATTATCTACAGCACGACAGTATAATCTTGCAGTTTTAATATTAAATCTTAATAATTCTTCTTTAGGTATGGTTAAACAA
TGGCAAGACATGATCTATTCTGGAAGACATTCACATTCATATATGGATTCACTTCCAGACTTCGTAAAATTAGTTGAATC
ATACGGACATATTGGTTTAAAGGTAAAAACAAATGAAGAATTAGAAGAAAAATTAATATTAGCATTAAAAAAACTATCTG
AAGGTAATTTAGTTTTTTTAGATATTCAAATAGATGATTCTGAGCATGTTTATCCTATGCAAATTCAAGGTGGTGGCATG
AATGAAATGTGGTTAAGGAAAAAAGAGGTTTCCTAA

Upstream 100 bases:

>100_bases
TTTAAATTATTTTTTTTTTATCTAGATAAACTATCAGGATAAACTACAAATAGAAAACTAGGAAATAAAACCTAATAAAT
TAGCATCCTGGAGGAAAAAA

Downstream 100 bases:

>100_bases
AAATATGCGAAGAATTTTATCTGTTCTCTTAGAGAATGAATCAGGTGCATTATCACGAGTCATAGGATTGTTTTCACAGC
GAGGTTACAATATAGAAACA

Product: acetolactate synthase large subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS

Number of amino acids: Translated: 571; Mature: 571

Protein sequence:

>571_residues
MEILSGAEMVIRSLINQGIQHIFGYPGGAVLDIYDALKTVGGVEHILVRHEQAATHMADGYARSTGKIGVVLVTSGPGAT
NAITGIATAYMDSIPMVVISGQVASSLIGYDAFQECDMIGISRPIVKHSFLVKRTEDIPIIFKKAFWLASTGRPGPVVID
LPKDILKKTNKYNFIWPKNIHIRSYNPTTKGHQGQIKKALRILLKAKKPIIYAGGGIISSNSSEELRIFAEKINCPVTTS
LMGLGAFPGNHIQSISMLGMHGTYEANMAMHYSDVIFAIGVRFDDRTTNNLKKYCPNATILHVDIDPTSISKTVSADIPI
VGDAKQVLKEMIELIKKEKQIHSLKEWWSSIGKWKKIKSLEYNKKSNKIKPQKIIQTLFKLTKGTSYITSDVGQHQMFTA
LYYQFNKPRRWINSGGLGTMGFGLPAALGVKLALPKATVICVTGDGSIQMNIQELSTARQYNLAVLILNLNNSSLGMVKQ
WQDMIYSGRHSHSYMDSLPDFVKLVESYGHIGLKVKTNEELEEKLILALKKLSEGNLVFLDIQIDDSEHVYPMQIQGGGM
NEMWLRKKEVS

Sequences:

>Translated_571_residues
MEILSGAEMVIRSLINQGIQHIFGYPGGAVLDIYDALKTVGGVEHILVRHEQAATHMADGYARSTGKIGVVLVTSGPGAT
NAITGIATAYMDSIPMVVISGQVASSLIGYDAFQECDMIGISRPIVKHSFLVKRTEDIPIIFKKAFWLASTGRPGPVVID
LPKDILKKTNKYNFIWPKNIHIRSYNPTTKGHQGQIKKALRILLKAKKPIIYAGGGIISSNSSEELRIFAEKINCPVTTS
LMGLGAFPGNHIQSISMLGMHGTYEANMAMHYSDVIFAIGVRFDDRTTNNLKKYCPNATILHVDIDPTSISKTVSADIPI
VGDAKQVLKEMIELIKKEKQIHSLKEWWSSIGKWKKIKSLEYNKKSNKIKPQKIIQTLFKLTKGTSYITSDVGQHQMFTA
LYYQFNKPRRWINSGGLGTMGFGLPAALGVKLALPKATVICVTGDGSIQMNIQELSTARQYNLAVLILNLNNSSLGMVKQ
WQDMIYSGRHSHSYMDSLPDFVKLVESYGHIGLKVKTNEELEEKLILALKKLSEGNLVFLDIQIDDSEHVYPMQIQGGGM
NEMWLRKKEVS
>Mature_571_residues
MEILSGAEMVIRSLINQGIQHIFGYPGGAVLDIYDALKTVGGVEHILVRHEQAATHMADGYARSTGKIGVVLVTSGPGAT
NAITGIATAYMDSIPMVVISGQVASSLIGYDAFQECDMIGISRPIVKHSFLVKRTEDIPIIFKKAFWLASTGRPGPVVID
LPKDILKKTNKYNFIWPKNIHIRSYNPTTKGHQGQIKKALRILLKAKKPIIYAGGGIISSNSSEELRIFAEKINCPVTTS
LMGLGAFPGNHIQSISMLGMHGTYEANMAMHYSDVIFAIGVRFDDRTTNNLKKYCPNATILHVDIDPTSISKTVSADIPI
VGDAKQVLKEMIELIKKEKQIHSLKEWWSSIGKWKKIKSLEYNKKSNKIKPQKIIQTLFKLTKGTSYITSDVGQHQMFTA
LYYQFNKPRRWINSGGLGTMGFGLPAALGVKLALPKATVICVTGDGSIQMNIQELSTARQYNLAVLILNLNNSSLGMVKQ
WQDMIYSGRHSHSYMDSLPDFVKLVESYGHIGLKVKTNEELEEKLILALKKLSEGNLVFLDIQIDDSEHVYPMQIQGGGM
NEMWLRKKEVS

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family

Homologues:

Organism=Homo sapiens, GI93004078, Length=569, Percent_Identity=27.0650263620387, Blast_Score=189, Evalue=5e-48,
Organism=Homo sapiens, GI21361361, Length=519, Percent_Identity=24.4701348747592, Blast_Score=161, Evalue=1e-39,
Organism=Escherichia coli, GI87081685, Length=572, Percent_Identity=70.8041958041958, Blast_Score=900, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=561, Percent_Identity=38.680926916221, Blast_Score=417, Evalue=1e-118,
Organism=Escherichia coli, GI1786717, Length=536, Percent_Identity=33.5820895522388, Blast_Score=320, Evalue=1e-88,
Organism=Escherichia coli, GI1787096, Length=566, Percent_Identity=25.6183745583039, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1788716, Length=541, Percent_Identity=24.9537892791128, Blast_Score=166, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17531299, Length=578, Percent_Identity=26.8166089965398, Blast_Score=163, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17531301, Length=578, Percent_Identity=26.8166089965398, Blast_Score=163, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17542570, Length=518, Percent_Identity=23.9382239382239, Blast_Score=133, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6323755, Length=580, Percent_Identity=41.7241379310345, Blast_Score=447, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6320816, Length=486, Percent_Identity=25.3086419753086, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6321524, Length=513, Percent_Identity=21.8323586744639, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19922626, Length=491, Percent_Identity=26.6802443991853, Blast_Score=181, Evalue=1e-45,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): ILVI_BUCAP (O85293)

Other databases:

- EMBL:   AF060492
- EMBL:   AE013218
- RefSeq:   NP_660569.1
- ProteinModelPortal:   O85293
- SMR:   O85293
- EnsemblBacteria:   EBBUCT00000000404
- GeneID:   1005419
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg220
- GeneTree:   EBGT00050000007943
- HOGENOM:   HBG323037
- OMA:   RLVFIDI
- ProtClustDB:   CLSK315806
- BioCyc:   BAPH198804:BUSG220-MONOMER
- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766
- TIGRFAMs:   TIGR00118

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N

EC number: =2.2.1.6

Molecular weight: Translated: 63268; Mature: 63268

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: PS00187 TPP_ENZYMES

Important sites: BINDING 51-51 BINDING 153-153

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEILSGAEMVIRSLINQGIQHIFGYPGGAVLDIYDALKTVGGVEHILVRHEQAATHMADG
CCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCC
YARSTGKIGVVLVTSGPGATNAITGIATAYMDSIPMVVISGQVASSLIGYDAFQECDMIG
CCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECHHHHHHHCCHHHHHCCCCC
ISRPIVKHSFLVKRTEDIPIIFKKAFWLASTGRPGPVVIDLPKDILKKTNKYNFIWPKNI
CCCHHHHHHHHEEECCCCCEEEEEEEEEECCCCCCCEEEECCHHHHHCCCCCEEECCCCE
HIRSYNPTTKGHQGQIKKALRILLKAKKPIIYAGGGIISSNSSEELRIFAEKINCPVTTS
EEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEECCEEEECCCCCHHEEEHHHCCCCEEHH
LMGLGAFPGNHIQSISMLGMHGTYEANMAMHYSDVIFAIGVRFDDRTTNNLKKYCPNATI
HHHCCCCCCCHHHHEEEEECCCCEECCEEEECCCEEEEEEEEECCCCHHHHHHHCCCCEE
LHVDIDPTSISKTVSADIPIVGDAKQVLKEMIELIKKEKQIHSLKEWWSSIGKWKKIKSL
EEEECCCCCCCHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EYNKKSNKIKPQKIIQTLFKLTKGTSYITSDVGQHQMFTALYYQFNKPRRWINSGGLGTM
CCCCCCCCCCHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHCCCHHHHCCCCCCCC
GFGLPAALGVKLALPKATVICVTGDGSIQMNIQELSTARQYNLAVLILNLNNSSLGMVKQ
CCCCHHHHCEEEECCCEEEEEEECCCCEEEEHHHHHHHHCCEEEEEEEEECCCCCHHHHH
WQDMIYSGRHSHSYMDSLPDFVKLVESYGHIGLKVKTNEELEEKLILALKKLSEGNLVFL
HHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHCCCCCEEEE
DIQIDDSEHVYPMQIQGGGMNEMWLRKKEVS
EEEECCCCCEEEEEECCCCCCHHHHHHCCCC
>Mature Secondary Structure
MEILSGAEMVIRSLINQGIQHIFGYPGGAVLDIYDALKTVGGVEHILVRHEQAATHMADG
CCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCC
YARSTGKIGVVLVTSGPGATNAITGIATAYMDSIPMVVISGQVASSLIGYDAFQECDMIG
CCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECHHHHHHHCCHHHHHCCCCC
ISRPIVKHSFLVKRTEDIPIIFKKAFWLASTGRPGPVVIDLPKDILKKTNKYNFIWPKNI
CCCHHHHHHHHEEECCCCCEEEEEEEEEECCCCCCCEEEECCHHHHHCCCCCEEECCCCE
HIRSYNPTTKGHQGQIKKALRILLKAKKPIIYAGGGIISSNSSEELRIFAEKINCPVTTS
EEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEECCEEEECCCCCHHEEEHHHCCCCEEHH
LMGLGAFPGNHIQSISMLGMHGTYEANMAMHYSDVIFAIGVRFDDRTTNNLKKYCPNATI
HHHCCCCCCCHHHHEEEEECCCCEECCEEEECCCEEEEEEEEECCCCHHHHHHHCCCCEE
LHVDIDPTSISKTVSADIPIVGDAKQVLKEMIELIKKEKQIHSLKEWWSSIGKWKKIKSL
EEEECCCCCCCHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EYNKKSNKIKPQKIIQTLFKLTKGTSYITSDVGQHQMFTALYYQFNKPRRWINSGGLGTM
CCCCCCCCCCHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHCCCHHHHCCCCCCCC
GFGLPAALGVKLALPKATVICVTGDGSIQMNIQELSTARQYNLAVLILNLNNSSLGMVKQ
CCCCHHHHCEEEECCCEEEEEEECCCCEEEEHHHHHHHHCCEEEEEEEEECCCCCHHHHH
WQDMIYSGRHSHSYMDSLPDFVKLVESYGHIGLKVKTNEELEEKLILALKKLSEGNLVFL
HHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHCCCCCEEEE
DIQIDDSEHVYPMQIQGGGMNEMWLRKKEVS
EEEECCCCCEEEEEECCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9688822; 12089438