Click here to switch to the map view.

The map label for this gene is glmU

Identifier: 21672321

GI number: 21672321

Start: 28115

End: 29500

Strand: Reverse

Name: glmU

Synonym: BUsg028

Alternate gene names: 21672321

Gene position: 29500-28115 (Counterclockwise)

Preceding gene: 21672323

Following gene: 21672320

Centisome position: 4.6

GC content: 25.69

Gene sequence:

>1386_bases
ATGTTAAAAAAAGAAATAAATGTAGTAATACTTGCAGCTGGAAAAGGAACAAGAATGCAATCTAGCTATCCTAAGGTATT
ACATAAATTAGGTGGAAAAACAATTTTAGAACATGTTATAAATATAGCAAAATCTGTTAAACCTAAAAAAATTATATTAG
TTTATAACAATAAAGAAAAAGAAATAAAATCTAAAATTAGTGACACTTCTATAGATTGGGTTATTCAAAAAGAACAAAAG
GGAACAGGGGATGCTATACTAAAAGCATCTAAAAAATTTTCAGATAAAGACGATATAGTAGTTCTTTATGGAGATATGCC
ATATATTTCAATTGAATCAATAAAAAAATTATTTACCTCTAAAAAACAATCTGATATTAGCTTATTGACTGCATACGTTA
AAAATCCTGATGGATACGGACGAGTCTTTAAAAAAAATGGGAAAGTTATTAAAATAATAGAAGAACAAGATGCTAATTTT
CACGAAAAAAAAATTAAAGAAGTATATTCTGGAACCTTCATAGCAAATGGAAAAGATCTAAAAAGATGGCTAAATCAAAT
TAATAATAAAAATATTAAAAAAGAATTTTATGCAACCGATATTGTCCATTTTGCTAATTTAGAAAACAGTACTATTAAAA
CAGTACAAGTTTTAAACTGTAAAGAAATATTAGGTGTAAATAATAAACTACAATTATCTATTTTAGAAAAAATTTTTCGA
AAAAAACAGGTTAATGATTTACTTCTTTCAGGTGTAACGTTAAAAGATCCAAATCATTTTATTTTAAGAGGAATATTAAA
ACATGGAAAAAATATAGAAATCGATACTGGTGTTATATTAGAAGGAAATATAATTTTAGGAAATAATATCAAAATTGGAG
TTGGATCTGTGATTAAAAATAGCTTTATTGACGATCAAACTGAAATTAAAGAATATACAATTATAGAAAACGTCAAAATA
GGCAAAAAATGTATTATAGGTCCTTTTGCGCATTTACGACCTAAAACTGTATTAGATGATCAAATTCATGTAGGTAATTT
TGTTGAAATTAAGGATAGTATCATAAAAAAAGAATCTAAGATTAAACACCTAAGTTATTTTGGAAATTCTGAAATTGGTT
CTCAAGTTAATATTGGAGCAGGTAGTATCACATGCAATTATGATGGTGTCAATAAATTCAAAACTATTATTGGTGATAAC
GTTTTAATAGGTGCTAACACAAAGTTAATTGCACCCATTAAAATTACGAAAAATGCGACGATAGCAGCAGGAACTACCTT
AACTCAAGACGTTAATACACCATGTTTGATTTATAATAATAAAGAACAAAAACAAAAAAAAAACTGGAAACGTCCTCAAA
AAATCATAAAAAAAACTGATCAATAA

Upstream 100 bases:

>100_bases
AAAATATATCATAAAAAATATAAAAATAATTTAAAATTTTGTAAAAATTAATGACTGAAATAAAAATTGCAATCTTATAA
AAATAAAAAGGAATATATAA

Downstream 100 bases:

>100_bases
TATTAAACAATTGATATATTTCATTGTGAAACTATTCAATAAAAATTAAAAAGGTATATTTCTATGTGTGGTATTGTTGC
TGCAGTAACACAACGTAACA

Product: UDP-N-acetylglucosamine pyrophosphorylase

Products: NA

Alternate protein names: UDP-N-acetylglucosamine pyrophosphorylase; N-acetylglucosamine-1-phosphate uridyltransferase; Glucosamine-1-phosphate N-acetyltransferase

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MLKKEINVVILAAGKGTRMQSSYPKVLHKLGGKTILEHVINIAKSVKPKKIILVYNNKEKEIKSKISDTSIDWVIQKEQK
GTGDAILKASKKFSDKDDIVVLYGDMPYISIESIKKLFTSKKQSDISLLTAYVKNPDGYGRVFKKNGKVIKIIEEQDANF
HEKKIKEVYSGTFIANGKDLKRWLNQINNKNIKKEFYATDIVHFANLENSTIKTVQVLNCKEILGVNNKLQLSILEKIFR
KKQVNDLLLSGVTLKDPNHFILRGILKHGKNIEIDTGVILEGNIILGNNIKIGVGSVIKNSFIDDQTEIKEYTIIENVKI
GKKCIIGPFAHLRPKTVLDDQIHVGNFVEIKDSIIKKESKIKHLSYFGNSEIGSQVNIGAGSITCNYDGVNKFKTIIGDN
VLIGANTKLIAPIKITKNATIAAGTTLTQDVNTPCLIYNNKEQKQKKNWKRPQKIIKKTDQ

Sequences:

>Translated_461_residues
MLKKEINVVILAAGKGTRMQSSYPKVLHKLGGKTILEHVINIAKSVKPKKIILVYNNKEKEIKSKISDTSIDWVIQKEQK
GTGDAILKASKKFSDKDDIVVLYGDMPYISIESIKKLFTSKKQSDISLLTAYVKNPDGYGRVFKKNGKVIKIIEEQDANF
HEKKIKEVYSGTFIANGKDLKRWLNQINNKNIKKEFYATDIVHFANLENSTIKTVQVLNCKEILGVNNKLQLSILEKIFR
KKQVNDLLLSGVTLKDPNHFILRGILKHGKNIEIDTGVILEGNIILGNNIKIGVGSVIKNSFIDDQTEIKEYTIIENVKI
GKKCIIGPFAHLRPKTVLDDQIHVGNFVEIKDSIIKKESKIKHLSYFGNSEIGSQVNIGAGSITCNYDGVNKFKTIIGDN
VLIGANTKLIAPIKITKNATIAAGTTLTQDVNTPCLIYNNKEQKQKKNWKRPQKIIKKTDQ
>Mature_461_residues
MLKKEINVVILAAGKGTRMQSSYPKVLHKLGGKTILEHVINIAKSVKPKKIILVYNNKEKEIKSKISDTSIDWVIQKEQK
GTGDAILKASKKFSDKDDIVVLYGDMPYISIESIKKLFTSKKQSDISLLTAYVKNPDGYGRVFKKNGKVIKIIEEQDANF
HEKKIKEVYSGTFIANGKDLKRWLNQINNKNIKKEFYATDIVHFANLENSTIKTVQVLNCKEILGVNNKLQLSILEKIFR
KKQVNDLLLSGVTLKDPNHFILRGILKHGKNIEIDTGVILEGNIILGNNIKIGVGSVIKNSFIDDQTEIKEYTIIENVKI
GKKCIIGPFAHLRPKTVLDDQIHVGNFVEIKDSIIKKESKIKHLSYFGNSEIGSQVNIGAGSITCNYDGVNKFKTIIGDN
VLIGANTKLIAPIKITKNATIAAGTTLTQDVNTPCLIYNNKEQKQKKNWKRPQKIIKKTDQ

Specific function: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-GlcNAc. Responsible for the acetylation of Glc-N-1-P to give GlcNAc-1-P and for the uridyl transfer from UTP to GlcNAc-1-P which produces UDP-GlcNAc

COG id: COG1207

COG function: function code M; N-acetylglucosamine-1-phosphate uridyltransferase (contains nucleotidyltransferase and I-patch acetyltransferase domains)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transferase hexapeptide repeat family

Homologues:

Organism=Escherichia coli, GI1790168, Length=454, Percent_Identity=49.7797356828194, Blast_Score=485, Evalue=1e-138,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLMU_BUCAP (Q8KA74)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660388.1
- ProteinModelPortal:   Q8KA74
- SMR:   Q8KA74
- EnsemblBacteria:   EBBUCT00000000480
- GeneID:   1005844
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg028
- GeneTree:   EBGT00050000008186
- HOGENOM:   HBG688195
- OMA:   GSKVNHL
- ProtClustDB:   CLSK493047
- BioCyc:   BAPH198804:BUSG028-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01631
- InterPro:   IPR005882
- InterPro:   IPR005835
- InterPro:   IPR011004
- TIGRFAMs:   TIGR01173

Pfam domain/function: PF00483 NTP_transferase; SSF51161 Trimer_LpxA_like

EC number: =2.7.7.23; =2.3.1.157

Molecular weight: Translated: 51735; Mature: 51735

Theoretical pI: Translated: 10.34; Mature: 10.34

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: ACT_SITE 363-363 BINDING 76-76 BINDING 140-140 BINDING 154-154 BINDING 387-387 BINDING 423-423

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKEINVVILAAGKGTRMQSSYPKVLHKLGGKTILEHVINIAKSVKPKKIILVYNNKEK
CCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCCHH
EIKSKISDTSIDWVIQKEQKGTGDAILKASKKFSDKDDIVVLYGDMPYISIESIKKLFTS
HHHHHHCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCCEEEEECCCCEEEHHHHHHHHHC
KKQSDISLLTAYVKNPDGYGRVFKKNGKVIKIIEEQDANFHEKKIKEVYSGTFIANGKDL
CCCCCHHHHHHHHCCCCCCCEEEECCCCEEEEEECCCCCHHHHHHHHHHCCCEEECCHHH
KRWLNQINNKNIKKEFYATDIVHFANLENSTIKTVQVLNCKEILGVNNKLQLSILEKIFR
HHHHHHHCCCCCHHHHHHHHHHHEECCCCCCEEEEEEECHHHHHCCCCEEHHHHHHHHHH
KKQVNDLLLSGVTLKDPNHFILRGILKHGKNIEIDTGVILEGNIILGNNIKIGVGSVIKN
HHHHHHHHHCCEEECCCCHHHHHHHHHCCCCEEECCCEEEECCEEECCCEEEEHHHHHHH
SFIDDQTEIKEYTIIENVKIGKKCIIGPFAHLRPKTVLDDQIHVGNFVEIKDSIIKKESK
HCCCCHHHHHHEEEEECCCCCCEEEECCHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHH
IKHLSYFGNSEIGSQVNIGAGSITCNYDGVNKFKTIIGDNVLIGANTKLIAPIKITKNAT
HHHHHHCCCCCCCCEEECCCCEEEEECCCHHHHHHHHCCCEEEECCCEEEEEEEEECCCE
IAAGTTLTQDVNTPCLIYNNKEQKQKKNWKRPQKIIKKTDQ
EEECCEEECCCCCCEEEECCHHHHHHHCCCCHHHHHHCCCC
>Mature Secondary Structure
MLKKEINVVILAAGKGTRMQSSYPKVLHKLGGKTILEHVINIAKSVKPKKIILVYNNKEK
CCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCCHH
EIKSKISDTSIDWVIQKEQKGTGDAILKASKKFSDKDDIVVLYGDMPYISIESIKKLFTS
HHHHHHCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCCEEEEECCCCEEEHHHHHHHHHC
KKQSDISLLTAYVKNPDGYGRVFKKNGKVIKIIEEQDANFHEKKIKEVYSGTFIANGKDL
CCCCCHHHHHHHHCCCCCCCEEEECCCCEEEEEECCCCCHHHHHHHHHHCCCEEECCHHH
KRWLNQINNKNIKKEFYATDIVHFANLENSTIKTVQVLNCKEILGVNNKLQLSILEKIFR
HHHHHHHCCCCCHHHHHHHHHHHEECCCCCCEEEEEEECHHHHHCCCCEEHHHHHHHHHH
KKQVNDLLLSGVTLKDPNHFILRGILKHGKNIEIDTGVILEGNIILGNNIKIGVGSVIKN
HHHHHHHHHCCEEECCCCHHHHHHHHHCCCCEEECCCEEEECCEEECCCEEEEHHHHHHH
SFIDDQTEIKEYTIIENVKIGKKCIIGPFAHLRPKTVLDDQIHVGNFVEIKDSIIKKESK
HCCCCHHHHHHEEEEECCCCCCEEEECCHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHH
IKHLSYFGNSEIGSQVNIGAGSITCNYDGVNKFKTIIGDNVLIGANTKLIAPIKITKNAT
HHHHHHCCCCCCCCEEECCCCEEEEECCCHHHHHHHHCCCEEEECCCEEEEEEEEECCCE
IAAGTTLTQDVNTPCLIYNNKEQKQKKNWKRPQKIIKKTDQ
EEECCEEECCCCCCEEEECCHHHHHHHCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438