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The map label for this gene is dnaA

Identifier: 21672306

GI number: 21672306

Start: 12397

End: 13761

Strand: Reverse

Name: dnaA

Synonym: BUsg012

Alternate gene names: 21672306

Gene position: 13761-12397 (Counterclockwise)

Preceding gene: 21672314

Following gene: 21672305

Centisome position: 2.15

GC content: 30.26

Gene sequence:

>1365_bases
GTGTCACTTTGTCTTTGGAAACAGTGTCTTGACCGGTTGCAGAGCGAGCTGCCATCTACAGAATTTAGTATGTGGATACG
CTCTCTAAAAGCCAAATTAAACAATAATATTTTAGAAATATATGCTCCAAATCAATTTATTTTAGATTGGGTAAAAGATA
AATATTTAATTCATTTTAAAAAAATACTACAAGATTTTTGTGGTTCTAATTCACCATTCATAAAATTTAAAGTATACCAA
ACTTCTAAAGAAAAAAAATTTAAAAAAAACATTTTACAAAAAATACAAAATTTAAATGCAAAACCAATTTGGGATAAGAT
ACCAATTTTTAAAAAATCGTCTCATCGTTCTAATATTAACAAAAAACATAGTTTTGAAAATTTTATAGAAGGAAAATCAA
ATCAATTAGCACGAGCAGCTGCATCTCAAGTAGCTAAAAACCCTGGAAACTCTTATAATCCACTTTTTTTATATGGAGGT
ACAGGATTAGGAAAAACACATTTATTACATGCTATAGGGAACGGAATATTAGCATATAAATATAATGTTAAAATTATCTA
TATGCATTCTGAACGTTTTGTGCAAGATATGGTCAAGGCTTTACAAAATAATGCAATTGAAAAATTTAAATTATACTATC
GTTCTGTTGACGCATTGTTAATTGACGATATTCAATTTTTTGCACATAAAGAACGTTCTCAAGAAGAATTTTTTCATACA
TTTAATGCTCTTTTAGAAGGGAATCAACAAATCATTTTAACTTCAGATCGATATCCTAAAGAAATTAATGGAGTTGAAGA
TCGTTTAAAATCGAGATTTGGTTGGGGTCTGACAGTTGCTATTGATCCACCAGAATTAGAAACAAGAGTCGCCATATTGA
TTAAAAAAGCAGATGAAAATAATATTGTATTATCTGACGAAGTTGCATTCTTTATAGCCAAACATTTACGTTCTAATGTT
CGCGAATTAGAAGGAGCTCTAAACAGAGTAATTGTTAATGCTAATTTTACACACCGTTCTATTACTGTAGAATTCGTTCG
TGAAGCGCTTCGAGATATATTAGCTTTGCAGGAAAAACTAGTTACCATTGCTAATATTCAAAAAACAGTTGCAGAATATT
ATAAAATAAAAGTAGCAGATTTATTATCTAGACGACGTTCTCGTTCAGTAGCTCGTCCCAGACAAATGGCGATGGCTATG
GCAAAAGAATTAACTAATCATAGCTTGCCAGAAATTGGAGATGCTTTTAGCGGAAGAGATCATACCACAGTACTTCATGC
TTGTCGTAAAATTGAACAATTACGTAAAGAAAATCACGATATTAAAGAAGATTTTTCAAATTTAATCAGAACTCTATCAG
TGTGA

Upstream 100 bases:

>100_bases
TATAATTTTTTTTTATATTAAACATATGTGTCAAAATAGAAAAACAAAAAAAACAGCCATTTTTTAAATTTCATTGAATT
TTGTTCGCTTGGAGTCTAGC

Downstream 100 bases:

>100_bases
TATTATGAAATTTACTATTCAAAACGATATTTTAACTAAAAATTTAAAAAAAATAACTCGAGTACTAGTCAAAAATATTT
CATTTCCTATTTTAGAAAAT

Product: chromosomal replication initiation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MSLCLWKQCLDRLQSELPSTEFSMWIRSLKAKLNNNILEIYAPNQFILDWVKDKYLIHFKKILQDFCGSNSPFIKFKVYQ
TSKEKKFKKNILQKIQNLNAKPIWDKIPIFKKSSHRSNINKKHSFENFIEGKSNQLARAAASQVAKNPGNSYNPLFLYGG
TGLGKTHLLHAIGNGILAYKYNVKIIYMHSERFVQDMVKALQNNAIEKFKLYYRSVDALLIDDIQFFAHKERSQEEFFHT
FNALLEGNQQIILTSDRYPKEINGVEDRLKSRFGWGLTVAIDPPELETRVAILIKKADENNIVLSDEVAFFIAKHLRSNV
RELEGALNRVIVNANFTHRSITVEFVREALRDILALQEKLVTIANIQKTVAEYYKIKVADLLSRRRSRSVARPRQMAMAM
AKELTNHSLPEIGDAFSGRDHTTVLHACRKIEQLRKENHDIKEDFSNLIRTLSV

Sequences:

>Translated_454_residues
MSLCLWKQCLDRLQSELPSTEFSMWIRSLKAKLNNNILEIYAPNQFILDWVKDKYLIHFKKILQDFCGSNSPFIKFKVYQ
TSKEKKFKKNILQKIQNLNAKPIWDKIPIFKKSSHRSNINKKHSFENFIEGKSNQLARAAASQVAKNPGNSYNPLFLYGG
TGLGKTHLLHAIGNGILAYKYNVKIIYMHSERFVQDMVKALQNNAIEKFKLYYRSVDALLIDDIQFFAHKERSQEEFFHT
FNALLEGNQQIILTSDRYPKEINGVEDRLKSRFGWGLTVAIDPPELETRVAILIKKADENNIVLSDEVAFFIAKHLRSNV
RELEGALNRVIVNANFTHRSITVEFVREALRDILALQEKLVTIANIQKTVAEYYKIKVADLLSRRRSRSVARPRQMAMAM
AKELTNHSLPEIGDAFSGRDHTTVLHACRKIEQLRKENHDIKEDFSNLIRTLSV
>Mature_453_residues
SLCLWKQCLDRLQSELPSTEFSMWIRSLKAKLNNNILEIYAPNQFILDWVKDKYLIHFKKILQDFCGSNSPFIKFKVYQT
SKEKKFKKNILQKIQNLNAKPIWDKIPIFKKSSHRSNINKKHSFENFIEGKSNQLARAAASQVAKNPGNSYNPLFLYGGT
GLGKTHLLHAIGNGILAYKYNVKIIYMHSERFVQDMVKALQNNAIEKFKLYYRSVDALLIDDIQFFAHKERSQEEFFHTF
NALLEGNQQIILTSDRYPKEINGVEDRLKSRFGWGLTVAIDPPELETRVAILIKKADENNIVLSDEVAFFIAKHLRSNVR
ELEGALNRVIVNANFTHRSITVEFVREALRDILALQEKLVTIANIQKTVAEYYKIKVADLLSRRRSRSVARPRQMAMAMA
KELTNHSLPEIGDAFSGRDHTTVLHACRKIEQLRKENHDIKEDFSNLIRTLSV

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family

Homologues:

Organism=Escherichia coli, GI2367267, Length=466, Percent_Identity=75.7510729613734, Blast_Score=742, Evalue=0.0,
Organism=Escherichia coli, GI226510964, Length=236, Percent_Identity=25, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNAA_BUCAP (P29434)

Other databases:

- EMBL:   M80817
- EMBL:   AF008210
- EMBL:   AE013218
- PIR:   JC1158
- RefSeq:   NP_660373.1
- ProteinModelPortal:   P29434
- EnsemblBacteria:   EBBUCT00000000118
- GeneID:   1005828
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg012
- GeneTree:   EBGT00050000008033
- HOGENOM:   HBG695666
- OMA:   DEVAFLI
- ProtClustDB:   PRK00149
- BioCyc:   BAPH198804:BUSG012-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00377
- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921
- Gene3D:   G3DSA:1.10.1750.10
- PRINTS:   PR00051
- SMART:   SM00382
- SMART:   SM00760
- TIGRFAMs:   TIGR00362

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C; SSF48295 Trp_repress_rep

EC number: NA

Molecular weight: Translated: 52527; Mature: 52396

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLCLWKQCLDRLQSELPSTEFSMWIRSLKAKLNNNILEIYAPNQFILDWVKDKYLIHFK
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHH
KILQDFCGSNSPFIKFKVYQTSKEKKFKKNILQKIQNLNAKPIWDKIPIFKKSSHRSNIN
HHHHHHCCCCCCEEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
KKHSFENFIEGKSNQLARAAASQVAKNPGNSYNPLFLYGGTGLGKTHLLHAIGNGILAYK
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHCCCEEEEE
YNVKIIYMHSERFVQDMVKALQNNAIEKFKLYYRSVDALLIDDIQFFAHKERSQEEFFHT
EEEEEEEECCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FNALLEGNQQIILTSDRYPKEINGVEDRLKSRFGWGLTVAIDPPELETRVAILIKKADEN
HHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEECCCC
NIVLSDEVAFFIAKHLRSNVRELEGALNRVIVNANFTHRSITVEFVREALRDILALQEKL
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
VTIANIQKTVAEYYKIKVADLLSRRRSRSVARPRQMAMAMAKELTNHSLPEIGDAFSGRD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC
HTTVLHACRKIEQLRKENHDIKEDFSNLIRTLSV
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLCLWKQCLDRLQSELPSTEFSMWIRSLKAKLNNNILEIYAPNQFILDWVKDKYLIHFK
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHH
KILQDFCGSNSPFIKFKVYQTSKEKKFKKNILQKIQNLNAKPIWDKIPIFKKSSHRSNIN
HHHHHHCCCCCCEEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
KKHSFENFIEGKSNQLARAAASQVAKNPGNSYNPLFLYGGTGLGKTHLLHAIGNGILAYK
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHCCCEEEEE
YNVKIIYMHSERFVQDMVKALQNNAIEKFKLYYRSVDALLIDDIQFFAHKERSQEEFFHT
EEEEEEEECCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FNALLEGNQQIILTSDRYPKEINGVEDRLKSRFGWGLTVAIDPPELETRVAILIKKADEN
HHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEECCCC
NIVLSDEVAFFIAKHLRSNVRELEGALNRVIVNANFTHRSITVEFVREALRDILALQEKL
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
VTIANIQKTVAEYYKIKVADLLSRRRSRSVARPRQMAMAMAKELTNHSLPEIGDAFSGRD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC
HTTVLHACRKIEQLRKENHDIKEDFSNLIRTLSV
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1572539; 9516544; 12089438