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The map label for this gene is dnaN

Identifier: 21672305

GI number: 21672305

Start: 11292

End: 12392

Strand: Reverse

Name: dnaN

Synonym: BUsg011

Alternate gene names: 21672305

Gene position: 12392-11292 (Counterclockwise)

Preceding gene: 21672306

Following gene: 21672304

Centisome position: 1.93

GC content: 21.62

Gene sequence:

>1101_bases
ATGAAATTTACTATTCAAAACGATATTTTAACTAAAAATTTAAAAAAAATAACTCGAGTACTAGTCAAAAATATTTCATT
TCCTATTTTAGAAAATATATTAATTCAAGTTGAAGATGGAACTTTGTCTTTAACAACAACAAATTTAGAAATAGAGCTTA
TTTCAAAAATTGAAATTATAACAAAATATATACCAGGAAAAACAACTATTTCAGGTAGAAAAATTTTAAATATTTGTCGA
ACTTTATCAGAAAAATCAAAAATAAAAATGCAACTAAAAAATAAAAAAATGTATATTTCTTCTGAAAATAGCAATTATAT
ATTAAGTACTTTATCTGCTGATACTTTTCCAAATCATCAAAATTTTGATTATATTTCCAAATTTGATATATCCTCAAATA
TATTAAAAGAAATGATAGAAAAAACTGAATTTTCAATGGGAAAACAAGATGTAAGATATTACTTAAATGGTATGTTATTA
GAAAAAAAAGATAAATTTTTACGTAGTGTTGCAACAGATGGATATAGATTAGCAATATCGTATACTCAATTAAAGAAAGA
TATCAATTTTTTTTCAATTATTATTCCTAATAAAGCAGTAATGGAATTGTTAAAATTATTAAATACTCAACCACAATTAT
TAAATATTTTAATTGGTTCCAATAGTATTAGAATATATACAAAAAATTTAATTTTTACTACTCAATTAATTGAAGGTGAA
TATCCTGATTATAAGAGTGTTTTATTTAAAGAAAAAAAGAATCCAATTATTACTAATTCTATTTTATTAAAAAAATCACT
ATTACGCGTTGCTATTCTAGCTCATGAAAAGTTCTGTGGAATTGAAATAAAAATTGAAAACGGTAAGTTTAAAGTACTAT
CTGATAATCAAGAAGAAGAAACTGCTGAAGATCTATTCGAAATTGATTATTTCGGAGAAAAAATAGAAATCTCAATAAAT
GTTTATTATCTATTAGATGTTATCAACAATATAAAAAGTGAAAATATTGCTTTATTTTTAAATAAATCTAAATCTTCTAT
ACAAATCGAAGCAGAAAACAATTCTTCAAACGCATATGTTGTAATGCTATTGAAAAGATAA

Upstream 100 bases:

>100_bases
GTACTTCATGCTTGTCGTAAAATTGAACAATTACGTAAAGAAAATCACGATATTAAAGAAGATTTTTCAAATTTAATCAG
AACTCTATCAGTGTGATATT

Downstream 100 bases:

>100_bases
AAATAAGTAATTTATATATATATCAAAGAAATTTAAGAACATAAATTTTAACATATTCAAAATATAAAAAATAATTAAAA
TTTTAAAAATAGAAAATATA

Product: DNA polymerase III beta chain

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MKFTIQNDILTKNLKKITRVLVKNISFPILENILIQVEDGTLSLTTTNLEIELISKIEIITKYIPGKTTISGRKILNICR
TLSEKSKIKMQLKNKKMYISSENSNYILSTLSADTFPNHQNFDYISKFDISSNILKEMIEKTEFSMGKQDVRYYLNGMLL
EKKDKFLRSVATDGYRLAISYTQLKKDINFFSIIIPNKAVMELLKLLNTQPQLLNILIGSNSIRIYTKNLIFTTQLIEGE
YPDYKSVLFKEKKNPIITNSILLKKSLLRVAILAHEKFCGIEIKIENGKFKVLSDNQEEETAEDLFEIDYFGEKIEISIN
VYYLLDVINNIKSENIALFLNKSKSSIQIEAENNSSNAYVVMLLKR

Sequences:

>Translated_366_residues
MKFTIQNDILTKNLKKITRVLVKNISFPILENILIQVEDGTLSLTTTNLEIELISKIEIITKYIPGKTTISGRKILNICR
TLSEKSKIKMQLKNKKMYISSENSNYILSTLSADTFPNHQNFDYISKFDISSNILKEMIEKTEFSMGKQDVRYYLNGMLL
EKKDKFLRSVATDGYRLAISYTQLKKDINFFSIIIPNKAVMELLKLLNTQPQLLNILIGSNSIRIYTKNLIFTTQLIEGE
YPDYKSVLFKEKKNPIITNSILLKKSLLRVAILAHEKFCGIEIKIENGKFKVLSDNQEEETAEDLFEIDYFGEKIEISIN
VYYLLDVINNIKSENIALFLNKSKSSIQIEAENNSSNAYVVMLLKR
>Mature_366_residues
MKFTIQNDILTKNLKKITRVLVKNISFPILENILIQVEDGTLSLTTTNLEIELISKIEIITKYIPGKTTISGRKILNICR
TLSEKSKIKMQLKNKKMYISSENSNYILSTLSADTFPNHQNFDYISKFDISSNILKEMIEKTEFSMGKQDVRYYLNGMLL
EKKDKFLRSVATDGYRLAISYTQLKKDINFFSIIIPNKAVMELLKLLNTQPQLLNILIGSNSIRIYTKNLIFTTQLIEGE
YPDYKSVLFKEKKNPIITNSILLKKSLLRVAILAHEKFCGIEIKIENGKFKVLSDNQEEETAEDLFEIDYFGEKIEISIN
VYYLLDVINNIKSENIALFLNKSKSSIQIEAENNSSNAYVVMLLKR

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=365, Percent_Identity=41.3698630136986, Blast_Score=328, Evalue=2e-91,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): DPO3B_BUCAP (P29439)

Other databases:

- EMBL:   M80817
- EMBL:   AF008210
- EMBL:   AE013218
- PIR:   JC1159
- RefSeq:   NP_660372.1
- ProteinModelPortal:   P29439
- SMR:   P29439
- EnsemblBacteria:   EBBUCT00000000517
- GeneID:   1005826
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg011
- GeneTree:   EBGT00050000008093
- HOGENOM:   HBG586514
- OMA:   DYNRVIP
- ProtClustDB:   PRK05643
- BioCyc:   BAPH198804:BUSG011-MONOMER
- GO:   GO:0005737
- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634
- Gene3D:   G3DSA:3.10.150.10
- SMART:   SM00480
- TIGRFAMs:   TIGR00663

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3

EC number: =2.7.7.7

Molecular weight: Translated: 42181; Mature: 42181

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTIQNDILTKNLKKITRVLVKNISFPILENILIQVEDGTLSLTTTNLEIELISKIEII
CEEEECHHHHHHHHHHHHHHHHHHCCHHHHCCEEEEEECCEEEEEEEEEEEEEHHHHHHH
TKYIPGKTTISGRKILNICRTLSEKSKIKMQLKNKKMYISSENSNYILSTLSADTFPNHQ
HHHCCCCCEECHHHHHHHHHHHCCCHHEEEEEECCEEEEECCCCCEEEEEECCCCCCCCC
NFDYISKFDISSNILKEMIEKTEFSMGKQDVRYYLNGMLLEKKDKFLRSVATDGYRLAIS
CCCCEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHCCEEEEHHHHHHHHHCCCCEEEEEE
YTQLKKDINFFSIIIPNKAVMELLKLLNTQPQLLNILIGSNSIRIYTKNLIFTTQLIEGE
HHHHHCCCCEEEEEECCHHHHHHHHHHCCCCHHEEEEECCCEEEEEEEEEEEEEEEECCC
YPDYKSVLFKEKKNPIITNSILLKKSLLRVAILAHEKFCGIEIKIENGKFKVLSDNQEEE
CCCHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEECCCCHHH
TAEDLFEIDYFGEKIEISINVYYLLDVINNIKSENIALFLNKSKSSIQIEAENNSSNAYV
HHHHHHHHHCCCCEEEEEEEEEEEEHHHHCCCCCCEEEEEECCCCEEEEEECCCCCCEEE
VMLLKR
EEEEEC
>Mature Secondary Structure
MKFTIQNDILTKNLKKITRVLVKNISFPILENILIQVEDGTLSLTTTNLEIELISKIEII
CEEEECHHHHHHHHHHHHHHHHHHCCHHHHCCEEEEEECCEEEEEEEEEEEEEHHHHHHH
TKYIPGKTTISGRKILNICRTLSEKSKIKMQLKNKKMYISSENSNYILSTLSADTFPNHQ
HHHCCCCCEECHHHHHHHHHHHCCCHHEEEEEECCEEEEECCCCCEEEEEECCCCCCCCC
NFDYISKFDISSNILKEMIEKTEFSMGKQDVRYYLNGMLLEKKDKFLRSVATDGYRLAIS
CCCCEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHCCEEEEHHHHHHHHHCCCCEEEEEE
YTQLKKDINFFSIIIPNKAVMELLKLLNTQPQLLNILIGSNSIRIYTKNLIFTTQLIEGE
HHHHHCCCCEEEEEECCHHHHHHHHHHCCCCHHEEEEECCCEEEEEEEEEEEEEEEECCC
YPDYKSVLFKEKKNPIITNSILLKKSLLRVAILAHEKFCGIEIKIENGKFKVLSDNQEEE
CCCHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEECCCCHHH
TAEDLFEIDYFGEKIEISINVYYLLDVINNIKSENIALFLNKSKSSIQIEAENNSSNAYV
HHHHHHHHHCCCCEEEEEEEEEEEEHHHHCCCCCCEEEEEECCCCEEEEEECCCCCCEEE
VMLLKR
EEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1572539; 9516544; 12089438