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The map label for this gene is gyrB

Identifier: 21672304

GI number: 21672304

Start: 8726

End: 11137

Strand: Reverse

Name: gyrB

Synonym: BUsg010

Alternate gene names: 21672304

Gene position: 11137-8726 (Counterclockwise)

Preceding gene: 21672305

Following gene: NA

Centisome position: 1.74

GC content: 26.16

Gene sequence:

>2412_bases
ATGATAGATACTTATGATTCTTCTAAAATAAAAATATTAAGAGGATTAGATGCCGTTCGTAAAAGACCAGGAATGTATAT
TGGAGATACTGATGATGGAAGTGGACTACATCATATGGTTTTCGAAATTGTTGATAATTCTATTGACGAAGCATTAGCAG
GGTTTTGTAAAGAAATCAAAGTAGTTATTCATTCTGATAATTCAGTATCAATTAAAGACGATGGAAGGGGTATTCCAACT
GATATACATCCTGAAGAAAAAATTTCAGCTGCAGAAGTTATTATGACAGTATTACACTCAGGTGGTAAGTTTGATAACGC
TTCTTATAAGATATCAGGTGGATTACATGGAGTAGGAATATCGGTTGTGAACGCTTTATCAGAAAAACTAGAATTAATAA
TTTATAAAAATAAAAAAAAATATCGACAACAATATAAAAACGGAAATCCAGAATCCCCTTTATGCATCATAGGTGCAACT
GAAATAACAGGAACATACATAAGATTTTGGCCTAGTTACAAAATTTTTACTAATAAAACACAATTTCAATATGAAATTTT
ATCTAAACGTTTACGCGAATTATCCTTTCTTAATTCTAACATATTGATTCACTTAAAAGACAATCGAACAAATTTAGAAG
AAAAATATCACTATAAGGGAGGTATTAAAGCATTTATCAAATTTTTAAATAAAAATAAATTCCCAATTAATCTAAATATA
TTTTATTTTCGATGTATTAAAAATCAAATAGAATTAGAAATCGCAATACAATGGAATAATTCACACCAAGAAAATATACT
TTGTTTCACTAATAACATACCCCAAAAAGATGGTGGAACACATTTATCTGGTTTTCGAGCAGGAATAACACGTACATTAA
ATTTACATATAGAACGCGAAGGGTACAATAAAAAAAACAAAACAATTATTACAGGTGAAGATACACGAGAGGGATTAACA
GCAATCATATCTATAAAAATACCTGATCCTAAATTTTCTTCTCAAACTAAAGATAAACTAGTTTCTTCTGAAGTAAAATC
AGTAATAGAATCATTAATTAACGAAAATCTTATCGAATATCTTTTAGAAAATCCTATTGATGCAAAGTTTATAATTCAAA
AAATCATAAACTCAGCTAAAATAAGAGAAGCAGCAAGACGAGCTAGAGAAATAAATAAAAAAAAGGGGACATTAGATTTA
GGTACGTTACCTGGAAAACTATCTGATTGTCAAGAAAATGATCCTAAATTATCAGAAATTTATTTAGTTGAGGGAGACTC
TGCAGGTGGATCAGCAAAACAAGGCAGAAACCGAAAAAATCAAGCTATTCTTCCTTTGAAAGGAAAAATATTAAATGTAC
AAAAAACAAAATTTGATAAAATAATCTTATCTCAAGAATTAACATCGCTTATAACAGCATTAGGATGTAGCATTACTAAA
AGTGAATATAGTTTAGATAAACTAAGATATAATCATATTATCATAATGACTGATGCTGATGTAGATGGAGCACATATTCG
AACACTATTATTAACATTCTTTTATCGTCAATTACCAGAATTAATTAAAAAAGGATATGTTTATATTGCTCAGCCACCTT
TATATAAGGTAAAAAAAGGAAAACAAGAAAAATATATTAAAAATGATGAAGAGATGAATAAATATCAAATTAAAATAGCT
TTAAAAGAAATTGTTATAAAAAACAAAAAAAATGAAAATTGCAATAAAATTATCAAAGAATTTCAAAAAATAGTATCTGA
TTTTAATCATATTCAAATTAAAATGAAAAAAAATAAAAATTATTTCCCTGAATTAATATTGAATGAACTGATCTATCATC
CTCGTTTATATAATTTAAAAAACGAAAACGTAGTTCAAAATTGGATAGAAAAACTAGTAGAAAAATTAAATAAAAAAGAT
AAAAATAATACAATATACACGTCAAAAATCAAGAGAAATGAAAATGATAGTATATTTGAACCAAGTATAAAATTATCAAG
ATATGCCAACCATACTCAATATGATTTAAAAAATGATTTTTTAGAAAGTAAAGAATATTTTTTAATTACTAATTTAGGTG
AAAAATTTAAAAAATTCCAAGAAAATGAAAATTTTATAGAAAAGGGAGAAAAAATTTATAAAATAAATGACATAAAAAAC
ACATTAGAATGGCTAATAAAAGAAACAAAACGCGGTTTTTTTGTACAACGTTATAAGGGACTTGGAGAAATGAATCCCGA
TCAACTATGGAATACAACAATGAATCCTGAAACACGAAATATGTTACAAGTAACTATCAAAGATGCTATTTCTGCTAATA
ATTTATTTAATACATTGATGGGAGACTTAGTAGAACCAAGAAGAAAATTTATACAAAAAAACGCTTTAAAAGCAGAAAAC
ATAGATATTTAA

Upstream 100 bases:

>100_bases
ATTCAAAATATAAAAAATAATTAAAATTTTAAAAATAGAAAATATATAATAATCGTATTATAAAAAACACATTATCAATT
AAAAAATAACAAGGAAAAAT

Downstream 100 bases:

>100_bases
AATATATAATTTAAAAAATTATAAAATATCTTAAATTTTTTTTTTAATTCTATAAACTATCTAAATAATGATTGCGGCAG
GAAAAAACCAGCCGCGAAAG

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 803; Mature: 803

Protein sequence:

>803_residues
MIDTYDSSKIKILRGLDAVRKRPGMYIGDTDDGSGLHHMVFEIVDNSIDEALAGFCKEIKVVIHSDNSVSIKDDGRGIPT
DIHPEEKISAAEVIMTVLHSGGKFDNASYKISGGLHGVGISVVNALSEKLELIIYKNKKKYRQQYKNGNPESPLCIIGAT
EITGTYIRFWPSYKIFTNKTQFQYEILSKRLRELSFLNSNILIHLKDNRTNLEEKYHYKGGIKAFIKFLNKNKFPINLNI
FYFRCIKNQIELEIAIQWNNSHQENILCFTNNIPQKDGGTHLSGFRAGITRTLNLHIEREGYNKKNKTIITGEDTREGLT
AIISIKIPDPKFSSQTKDKLVSSEVKSVIESLINENLIEYLLENPIDAKFIIQKIINSAKIREAARRAREINKKKGTLDL
GTLPGKLSDCQENDPKLSEIYLVEGDSAGGSAKQGRNRKNQAILPLKGKILNVQKTKFDKIILSQELTSLITALGCSITK
SEYSLDKLRYNHIIIMTDADVDGAHIRTLLLTFFYRQLPELIKKGYVYIAQPPLYKVKKGKQEKYIKNDEEMNKYQIKIA
LKEIVIKNKKNENCNKIIKEFQKIVSDFNHIQIKMKKNKNYFPELILNELIYHPRLYNLKNENVVQNWIEKLVEKLNKKD
KNNTIYTSKIKRNENDSIFEPSIKLSRYANHTQYDLKNDFLESKEYFLITNLGEKFKKFQENENFIEKGEKIYKINDIKN
TLEWLIKETKRGFFVQRYKGLGEMNPDQLWNTTMNPETRNMLQVTIKDAISANNLFNTLMGDLVEPRRKFIQKNALKAEN
IDI

Sequences:

>Translated_803_residues
MIDTYDSSKIKILRGLDAVRKRPGMYIGDTDDGSGLHHMVFEIVDNSIDEALAGFCKEIKVVIHSDNSVSIKDDGRGIPT
DIHPEEKISAAEVIMTVLHSGGKFDNASYKISGGLHGVGISVVNALSEKLELIIYKNKKKYRQQYKNGNPESPLCIIGAT
EITGTYIRFWPSYKIFTNKTQFQYEILSKRLRELSFLNSNILIHLKDNRTNLEEKYHYKGGIKAFIKFLNKNKFPINLNI
FYFRCIKNQIELEIAIQWNNSHQENILCFTNNIPQKDGGTHLSGFRAGITRTLNLHIEREGYNKKNKTIITGEDTREGLT
AIISIKIPDPKFSSQTKDKLVSSEVKSVIESLINENLIEYLLENPIDAKFIIQKIINSAKIREAARRAREINKKKGTLDL
GTLPGKLSDCQENDPKLSEIYLVEGDSAGGSAKQGRNRKNQAILPLKGKILNVQKTKFDKIILSQELTSLITALGCSITK
SEYSLDKLRYNHIIIMTDADVDGAHIRTLLLTFFYRQLPELIKKGYVYIAQPPLYKVKKGKQEKYIKNDEEMNKYQIKIA
LKEIVIKNKKNENCNKIIKEFQKIVSDFNHIQIKMKKNKNYFPELILNELIYHPRLYNLKNENVVQNWIEKLVEKLNKKD
KNNTIYTSKIKRNENDSIFEPSIKLSRYANHTQYDLKNDFLESKEYFLITNLGEKFKKFQENENFIEKGEKIYKINDIKN
TLEWLIKETKRGFFVQRYKGLGEMNPDQLWNTTMNPETRNMLQVTIKDAISANNLFNTLMGDLVEPRRKFIQKNALKAEN
IDI
>Mature_803_residues
MIDTYDSSKIKILRGLDAVRKRPGMYIGDTDDGSGLHHMVFEIVDNSIDEALAGFCKEIKVVIHSDNSVSIKDDGRGIPT
DIHPEEKISAAEVIMTVLHSGGKFDNASYKISGGLHGVGISVVNALSEKLELIIYKNKKKYRQQYKNGNPESPLCIIGAT
EITGTYIRFWPSYKIFTNKTQFQYEILSKRLRELSFLNSNILIHLKDNRTNLEEKYHYKGGIKAFIKFLNKNKFPINLNI
FYFRCIKNQIELEIAIQWNNSHQENILCFTNNIPQKDGGTHLSGFRAGITRTLNLHIEREGYNKKNKTIITGEDTREGLT
AIISIKIPDPKFSSQTKDKLVSSEVKSVIESLINENLIEYLLENPIDAKFIIQKIINSAKIREAARRAREINKKKGTLDL
GTLPGKLSDCQENDPKLSEIYLVEGDSAGGSAKQGRNRKNQAILPLKGKILNVQKTKFDKIILSQELTSLITALGCSITK
SEYSLDKLRYNHIIIMTDADVDGAHIRTLLLTFFYRQLPELIKKGYVYIAQPPLYKVKKGKQEKYIKNDEEMNKYQIKIA
LKEIVIKNKKNENCNKIIKEFQKIVSDFNHIQIKMKKNKNYFPELILNELIYHPRLYNLKNENVVQNWIEKLVEKLNKKD
KNNTIYTSKIKRNENDSIFEPSIKLSRYANHTQYDLKNDFLESKEYFLITNLGEKFKKFQENENFIEKGEKIYKINDIKN
TLEWLIKETKRGFFVQRYKGLGEMNPDQLWNTTMNPETRNMLQVTIKDAISANNLFNTLMGDLVEPRRKFIQKNALKAEN
IDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family

Homologues:

Organism=Homo sapiens, GI19913406, Length=555, Percent_Identity=24.6846846846847, Blast_Score=123, Evalue=7e-28,
Organism=Homo sapiens, GI19913408, Length=564, Percent_Identity=26.063829787234, Blast_Score=119, Evalue=9e-27,
Organism=Escherichia coli, GI48994957, Length=806, Percent_Identity=62.531017369727, Blast_Score=1068, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=597, Percent_Identity=36.1809045226131, Blast_Score=386, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17535065, Length=546, Percent_Identity=25.2747252747253, Blast_Score=119, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI212645845, Length=518, Percent_Identity=23.3590733590734, Blast_Score=117, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI212645657, Length=159, Percent_Identity=34.5911949685535, Blast_Score=78, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324241, Length=592, Percent_Identity=26.0135135135135, Blast_Score=118, Evalue=3e-27,
Organism=Drosophila melanogaster, GI17136538, Length=533, Percent_Identity=26.078799249531, Blast_Score=119, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GYRB_BUCAP (P29435)

Other databases:

- EMBL:   AF008210
- EMBL:   AE013218
- EMBL:   M80817
- RefSeq:   NP_660371.1
- ProteinModelPortal:   P29435
- EnsemblBacteria:   EBBUCT00000000326
- GeneID:   1005827
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg010
- GeneTree:   EBGT00050000008102
- HOGENOM:   HBG304889
- OMA:   DCSSRDP
- ProtClustDB:   PRK14939
- BioCyc:   BAPH198804:BUSG010-MONOMER
- GO:   GO:0005694
- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- Gene3D:   G3DSA:3.30.565.10
- Gene3D:   G3DSA:3.30.230.10
- Gene3D:   G3DSA:3.40.50.670
- PRINTS:   PR00418
- SMART:   SM00387
- SMART:   SM00433
- TIGRFAMs:   TIGR01059

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; SSF55874 ATP_bd_ATPase; SSF54211 Ribosomal_S5_D2-typ_fold; SSF56719 Topo_IIA_cen

EC number: =5.99.1.3

Molecular weight: Translated: 92679; Mature: 92679

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: PS00177 TOPOISOMERASE_II; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDTYDSSKIKILRGLDAVRKRPGMYIGDTDDGSGLHHMVFEIVDNSIDEALAGFCKEIK
CCCCCCCCCHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHEE
VVIHSDNSVSIKDDGRGIPTDIHPEEKISAAEVIMTVLHSGGKFDNASYKISGGLHGVGI
EEEECCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHH
SVVNALSEKLELIIYKNKKKYRQQYKNGNPESPLCIIGATEITGTYIRFWPSYKIFTNKT
HHHHHHHHHEEEEEECCHHHHHHHHCCCCCCCCEEEEECHHHCCEEEEEECCEEEEECCC
QFQYEILSKRLRELSFLNSNILIHLKDNRTNLEEKYHYKGGIKAFIKFLNKNKFPINLNI
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHCCCHHHHHHHHCCCCCEEEEEE
FYFRCIKNQIELEIAIQWNNSHQENILCFTNNIPQKDGGTHLSGFRAGITRTLNLHIERE
EEEEEECCCEEEEEEEEECCCCCCCEEEEECCCCCCCCCCCCHHHHHCCEEEEEEEEEEC
GYNKKNKTIITGEDTREGLTAIISIKIPDPKFSSQTKDKLVSSEVKSVIESLINENLIEY
CCCCCCCEEEECCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LLENPIDAKFIIQKIINSAKIREAARRAREINKKKGTLDLGTLPGKLSDCQENDPKLSEI
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCEEE
YLVEGDSAGGSAKQGRNRKNQAILPLKGKILNVQKTKFDKIILSQELTSLITALGCSITK
EEEECCCCCCCCCCCCCCCCCEEEECCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCC
SEYSLDKLRYNHIIIMTDADVDGAHIRTLLLTFFYRQLPELIKKGYVYIAQPPLYKVKKG
CHHHHHHEEECEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHCC
KQEKYIKNDEEMNKYQIKIALKEIVIKNKKNENCNKIIKEFQKIVSDFNHIQIKMKKNKN
CCHHHCCCCCCCCCEEEEEEHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCC
YFPELILNELIYHPRLYNLKNENVVQNWIEKLVEKLNKKDKNNTIYTSKIKRNENDSIFE
CCHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCEEC
PSIKLSRYANHTQYDLKNDFLESKEYFLITNLGEKFKKFQENENFIEKGEKIYKINDIKN
CCHHHHHHCCCCCCHHHHHHHCCCCEEEEECHHHHHHHHHCCCHHHHCCCEEEEEHHHHH
TLEWLIKETKRGFFVQRYKGLGEMNPDQLWNTTMNPETRNMLQVTIKDAISANNLFNTLM
HHHHHHHHHHCCHHHHHHHCCCCCCHHHHCCCCCCCCCCHHEEEEHHHHHHHHHHHHHHH
GDLVEPRRKFIQKNALKAENIDI
HHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MIDTYDSSKIKILRGLDAVRKRPGMYIGDTDDGSGLHHMVFEIVDNSIDEALAGFCKEIK
CCCCCCCCCHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHEE
VVIHSDNSVSIKDDGRGIPTDIHPEEKISAAEVIMTVLHSGGKFDNASYKISGGLHGVGI
EEEECCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHH
SVVNALSEKLELIIYKNKKKYRQQYKNGNPESPLCIIGATEITGTYIRFWPSYKIFTNKT
HHHHHHHHHEEEEEECCHHHHHHHHCCCCCCCCEEEEECHHHCCEEEEEECCEEEEECCC
QFQYEILSKRLRELSFLNSNILIHLKDNRTNLEEKYHYKGGIKAFIKFLNKNKFPINLNI
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHCCCHHHHHHHHCCCCCEEEEEE
FYFRCIKNQIELEIAIQWNNSHQENILCFTNNIPQKDGGTHLSGFRAGITRTLNLHIERE
EEEEEECCCEEEEEEEEECCCCCCCEEEEECCCCCCCCCCCCHHHHHCCEEEEEEEEEEC
GYNKKNKTIITGEDTREGLTAIISIKIPDPKFSSQTKDKLVSSEVKSVIESLINENLIEY
CCCCCCCEEEECCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LLENPIDAKFIIQKIINSAKIREAARRAREINKKKGTLDLGTLPGKLSDCQENDPKLSEI
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCEEE
YLVEGDSAGGSAKQGRNRKNQAILPLKGKILNVQKTKFDKIILSQELTSLITALGCSITK
EEEECCCCCCCCCCCCCCCCCEEEECCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCC
SEYSLDKLRYNHIIIMTDADVDGAHIRTLLLTFFYRQLPELIKKGYVYIAQPPLYKVKKG
CHHHHHHEEECEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHCC
KQEKYIKNDEEMNKYQIKIALKEIVIKNKKNENCNKIIKEFQKIVSDFNHIQIKMKKNKN
CCHHHCCCCCCCCCEEEEEEHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCC
YFPELILNELIYHPRLYNLKNENVVQNWIEKLVEKLNKKDKNNTIYTSKIKRNENDSIFE
CCHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCEEC
PSIKLSRYANHTQYDLKNDFLESKEYFLITNLGEKFKKFQENENFIEKGEKIYKINDIKN
CCHHHHHHCCCCCCHHHHHHHCCCCEEEEECHHHHHHHHHCCCHHHHCCCEEEEEHHHHH
TLEWLIKETKRGFFVQRYKGLGEMNPDQLWNTTMNPETRNMLQVTIKDAISANNLFNTLM
HHHHHHHHHHCCHHHHHHHCCCCCCHHHHCCCCCCCCCCHHEEEEHHHHHHHHHHHHHHH
GDLVEPRRKFIQKNALKAENIDI
HHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9516544; 12089438; 1572539