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The map label for this gene is ksgA [H]

Identifier: 213693330

GI number: 213693330

Start: 2813733

End: 2814656

Strand: Direct

Name: ksgA [H]

Synonym: Blon_2484

Alternate gene names: 213693330

Gene position: 2813733-2814656 (Clockwise)

Preceding gene: 213693329

Following gene: 213693331

Centisome position: 99.33

GC content: 60.71

Gene sequence:

>924_bases
ATGAACGACACCATTCCCGCCACCGGTCACCTGTTAGGTGCCGCCGATATCCGTCGTATCGCGGCCGATGCCGGCATCAG
CCCCACCAAGAAATTCGGGCAGAACTTCGTCATCGACCCCGGCACGGTCCGTCGTATCGTACGCGAGGCGGGAGTGACCG
CTGCCGATCATGTCATGGAAGTCGGCCCCGGCCTGGGATCGTTGACATTGGCGATTCTGGAAACCGGCGCCACCATGACC
GCAGTTGAAATCGATCCGCCGTTGGCCGAACGACTGCCGGGCACAGTCGCCGAATTCATGCCCGAAGCCACGTCACGACT
CACCGTCGTCAATCGGGATGCCTTAACCGTCACCCCGGAGAACGTGCCTGATTTCAGCGATGACGCATCGTTTACGCTGG
TCGCCAATCTGCCGTACAACGTGGCCACACCAATTCTGCTCACGCTGTTGGAGCGTTTCGACAACCTCGGCTCGTTCTTG
GTGATGGTGCAAAAAGAGGTGGCCGATAGACTCGCCGCCAAGCCCGGTTCCAAGATCTACGGCACGCCGAGCGTGAAGCT
CGCCTGGTATGGCACCGCCGAACGGGTGGGCACCATCGGCCGCAATGTGTTCTGGCCGGCTCCCAATGTGGATTCCGCGC
TCGTCAAATTCACCCGGTATCAGGCCGATGACCCGGCTGCCCCGGGAACTGCGAACAGCACAGACGACGGCACACAACGG
GAACTCGTATTTCGTCTGATCGATGCGGCATTCGGTCAGCGTCGCAAGACCCTGCACGCGGCATTGAAGACCATTGCGCC
TTCCGAGGCATTCTCCATCGCCGGCATCGATCCGACCCGTCGAGGAGAGACGCTCACCATCGCCGAATTCACCGCTCTGG
CCAAGGCCATCGAATCGTGTGGAGATGGAGATGAGGCACAATGA

Upstream 100 bases:

>100_bases
ACATCGCACAAACCTACGGCAGCCCGTCCAAGGCATGGCAACATTCCGAACAAATCGGTTGGTACTGATCGGTTACGAAG
CCCACAAGGAACACACGCTT

Downstream 100 bases:

>100_bases
GTCCGGTAATTTCACATCCACGCCCAGCCGCTGCCCGACATCAGTTCGATGAGCTCTCCCCCATCACCATTACGGTGGAT
TGCCCGGCTAAAACCAATCT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MNDTIPATGHLLGAADIRRIAADAGISPTKKFGQNFVIDPGTVRRIVREAGVTAADHVMEVGPGLGSLTLAILETGATMT
AVEIDPPLAERLPGTVAEFMPEATSRLTVVNRDALTVTPENVPDFSDDASFTLVANLPYNVATPILLTLLERFDNLGSFL
VMVQKEVADRLAAKPGSKIYGTPSVKLAWYGTAERVGTIGRNVFWPAPNVDSALVKFTRYQADDPAAPGTANSTDDGTQR
ELVFRLIDAAFGQRRKTLHAALKTIAPSEAFSIAGIDPTRRGETLTIAEFTALAKAIESCGDGDEAQ

Sequences:

>Translated_307_residues
MNDTIPATGHLLGAADIRRIAADAGISPTKKFGQNFVIDPGTVRRIVREAGVTAADHVMEVGPGLGSLTLAILETGATMT
AVEIDPPLAERLPGTVAEFMPEATSRLTVVNRDALTVTPENVPDFSDDASFTLVANLPYNVATPILLTLLERFDNLGSFL
VMVQKEVADRLAAKPGSKIYGTPSVKLAWYGTAERVGTIGRNVFWPAPNVDSALVKFTRYQADDPAAPGTANSTDDGTQR
ELVFRLIDAAFGQRRKTLHAALKTIAPSEAFSIAGIDPTRRGETLTIAEFTALAKAIESCGDGDEAQ
>Mature_307_residues
MNDTIPATGHLLGAADIRRIAADAGISPTKKFGQNFVIDPGTVRRIVREAGVTAADHVMEVGPGLGSLTLAILETGATMT
AVEIDPPLAERLPGTVAEFMPEATSRLTVVNRDALTVTPENVPDFSDDASFTLVANLPYNVATPILLTLLERFDNLGSFL
VMVQKEVADRLAAKPGSKIYGTPSVKLAWYGTAERVGTIGRNVFWPAPNVDSALVKFTRYQADDPAAPGTANSTDDGTQR
ELVFRLIDAAFGQRRKTLHAALKTIAPSEAFSIAGIDPTRRGETLTIAEFTALAKAIESCGDGDEAQ

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI156415992, Length=312, Percent_Identity=30.1282051282051, Blast_Score=111, Evalue=7e-25,
Organism=Homo sapiens, GI7657198, Length=234, Percent_Identity=32.4786324786325, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI1786236, Length=270, Percent_Identity=31.1111111111111, Blast_Score=131, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI25146882, Length=235, Percent_Identity=33.1914893617021, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI25141369, Length=306, Percent_Identity=29.4117647058824, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324989, Length=260, Percent_Identity=27.6923076923077, Blast_Score=100, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21358017, Length=253, Percent_Identity=32.0158102766798, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21357273, Length=303, Percent_Identity=26.0726072607261, Blast_Score=74, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 32619; Mature: 32619

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDTIPATGHLLGAADIRRIAADAGISPTKKFGQNFVIDPGTVRRIVREAGVTAADHVME
CCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCEEECCHHHHHHHHHHCCCHHHHHHH
VGPGLGSLTLAILETGATMTAVEIDPPLAERLPGTVAEFMPEATSRLTVVNRDALTVTPE
HCCCCHHHHHHHHHCCCEEEEEECCCCHHHHCCCHHHHHHHHHHHEEEEEECCEEEECCC
NVPDFSDDASFTLVANLPYNVATPILLTLLERFDNLGSFLVMVQKEVADRLAAKPGSKIY
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
GTPSVKLAWYGTAERVGTIGRNVFWPAPNVDSALVKFTRYQADDPAAPGTANSTDDGTQR
CCCCEEEEEECCHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHH
ELVFRLIDAAFGQRRKTLHAALKTIAPSEAFSIAGIDPTRRGETLTIAEFTALAKAIESC
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEEHHHHHHHHHHHHHC
GDGDEAQ
CCCCCCC
>Mature Secondary Structure
MNDTIPATGHLLGAADIRRIAADAGISPTKKFGQNFVIDPGTVRRIVREAGVTAADHVME
CCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCEEECCHHHHHHHHHHCCCHHHHHHH
VGPGLGSLTLAILETGATMTAVEIDPPLAERLPGTVAEFMPEATSRLTVVNRDALTVTPE
HCCCCHHHHHHHHHCCCEEEEEECCCCHHHHCCCHHHHHHHHHHHEEEEEECCEEEECCC
NVPDFSDDASFTLVANLPYNVATPILLTLLERFDNLGSFLVMVQKEVADRLAAKPGSKIY
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
GTPSVKLAWYGTAERVGTIGRNVFWPAPNVDSALVKFTRYQADDPAAPGTANSTDDGTQR
CCCCEEEEEECCHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHH
ELVFRLIDAAFGQRRKTLHAALKTIAPSEAFSIAGIDPTRRGETLTIAEFTALAKAIESC
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEEHHHHHHHHHHHHHC
GDGDEAQ
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12381787 [H]