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The map label for this gene is gcvH [H]

Identifier: 213692929

GI number: 213692929

Start: 2290481

End: 2290894

Strand: Reverse

Name: gcvH [H]

Synonym: Blon_2073

Alternate gene names: 213692929

Gene position: 2290894-2290481 (Counterclockwise)

Preceding gene: 213692930

Following gene: 213692928

Centisome position: 80.87

GC content: 61.35

Gene sequence:

>414_bases
ATGACTATGAGTGATGACAACGAAAACGAACAGCCGCTGAACCTCGACATTCCCGACCATCTCGAATACTCCGAGGAGCA
TGCGTGGGTGGACCGTTCGGTCGACCCCGCAATCATCGGCATCACCGAATACGCCGCCGACCAGCTTGGCGAACTTGTGT
TCGTGGATCTGCCTGAGCCTGGCGCCCGTGTGGAGGCCGGCGACGAGATCGTGGAGCTTGAATCCTCCAAGGCCGTCCAG
CCGTTGATCAGCCCCGTGGCGGGTACGGTCAAGTATGTGAACCGTGACGTGGCGGACGACCCGTCCGTGGTCAACGGCGA
TCCGTACGGCGAAGGCTGGCTGCTGAAGGTCGAGCTTGACGACGATGAGCCGGAGCTGCTGAGCGCCGACGAATACGCGA
ATGTCGTGCGGTAG

Upstream 100 bases:

>100_bases
GGCCGTATGGCGGCGCCGGGCCGCGCCACCATCGCCCGCTACATGATTGAGGAATGGCTCGGTCGCGAGCTGTGACGGAC
CGCGAAGATAGGTTAGGCTT

Downstream 100 bases:

>100_bases
TTCTACCCATGGCTCCCCCCCCCTGAGGGGAGCTGGCTATGAAGCGGACTGAGGGGAGTTTCCATATGTTGGGGACTCCC
CTCAGTCGCCCATGGCGACA

Product: glycine cleavage system H protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 137; Mature: 136

Protein sequence:

>137_residues
MTMSDDNENEQPLNLDIPDHLEYSEEHAWVDRSVDPAIIGITEYAADQLGELVFVDLPEPGARVEAGDEIVELESSKAVQ
PLISPVAGTVKYVNRDVADDPSVVNGDPYGEGWLLKVELDDDEPELLSADEYANVVR

Sequences:

>Translated_137_residues
MTMSDDNENEQPLNLDIPDHLEYSEEHAWVDRSVDPAIIGITEYAADQLGELVFVDLPEPGARVEAGDEIVELESSKAVQ
PLISPVAGTVKYVNRDVADDPSVVNGDPYGEGWLLKVELDDDEPELLSADEYANVVR
>Mature_136_residues
TMSDDNENEQPLNLDIPDHLEYSEEHAWVDRSVDPAIIGITEYAADQLGELVFVDLPEPGARVEAGDEIVELESSKAVQP
LISPVAGTVKYVNRDVADDPSVVNGDPYGEGWLLKVELDDDEPELLSADEYANVVR

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=118, Percent_Identity=34.7457627118644, Blast_Score=84, Evalue=5e-17,
Organism=Homo sapiens, GI89057342, Length=118, Percent_Identity=33.8983050847458, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1789271, Length=120, Percent_Identity=42.5, Blast_Score=99, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17551294, Length=112, Percent_Identity=35.7142857142857, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17507493, Length=117, Percent_Identity=34.1880341880342, Blast_Score=85, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6319272, Length=118, Percent_Identity=36.4406779661017, Blast_Score=90, Evalue=1e-19,
Organism=Drosophila melanogaster, GI17865652, Length=114, Percent_Identity=42.1052631578947, Blast_Score=89, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 15066; Mature: 14934

Theoretical pI: Translated: 3.58; Mature: 3.58

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMSDDNENEQPLNLDIPDHLEYSEEHAWVDRSVDPAIIGITEYAADQLGELVFVDLPEP
CCCCCCCCCCCCEECCCCCCCCCCCCHHCCCCCCCCEEEEHHHHHHHHCCCEEEEECCCC
GARVEAGDEIVELESSKAVQPLISPVAGTVKYVNRDVADDPSVVNGDPYGEGWLLKVELD
CCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEC
DDEPELLSADEYANVVR
CCCCCEECCHHHHHHCC
>Mature Secondary Structure 
TMSDDNENEQPLNLDIPDHLEYSEEHAWVDRSVDPAIIGITEYAADQLGELVFVDLPEP
CCCCCCCCCCCEECCCCCCCCCCCCHHCCCCCCCCEEEEHHHHHHHHCCCEEEEECCCC
GARVEAGDEIVELESSKAVQPLISPVAGTVKYVNRDVADDPSVVNGDPYGEGWLLKVELD
CCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEC
DDEPELLSADEYANVVR
CCCCCEECCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12381787 [H]