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The map label for this gene is regA [H]

Identifier: 213692913

GI number: 213692913

Start: 2268144

End: 2269235

Strand: Reverse

Name: regA [H]

Synonym: Blon_2057

Alternate gene names: 213692913

Gene position: 2269235-2268144 (Counterclockwise)

Preceding gene: 213692914

Following gene: 213692912

Centisome position: 80.11

GC content: 54.58

Gene sequence:

>1092_bases
ATGGTGCAGTCGTCAGTAAGTCAACGAGTGAACAGGTCCAAACGATTGGTATCCATCAAAGACATAGCTAAGGAAGCGGG
TGTTTCCGTATGCACTGTGTCTTTGGTTATGAACAACAAGGATCACAATCGCGTCAGCAAGGCGGTTGCGCAGAAAATCC
GTGCGACTGCCAAGAACATGGGGTACAAGCCCAATTCTGTGGCGCGCACCCTACGTACCAGCAAATCCAATACTCTGGGA
TTCGTCACCGATGAGATCGCCACGACCCCGTACGCGAGCAAGGTACTCGAAGGTGCGCAGGATGCTGCGAGCAAGCTCGG
ATACATACTGCTTACCACGAACACCAATAATGACGCTGAACTCGAGAACCAAGAAATATCCGCCCTGCTCCGATACAACG
TGGATGGCTTCCTATACGCCATGATGTTCCACCGCAAAGTGGACATTCCCGAAGTCTTGGAAGGCAGCCCGACCGTGGTG
GTGGACAGTGAGGATGCAGCGGGGCGAGTTCCCTCAGTCTACCCCGATGATGAACTGGCGGGGTATGACGCCACCGACTG
TCTGATCAAAGCTGGATGCAGACGCATTGTCTACTACGGTTCCGCAACTCCCATCGTCGCGCAAGGTCAACGTCTGACTG
GTTACCGACGTGCGCTTGCGGAAGCGGGCATTCCATTCGATGACGCTTTGGTCGTCAATGTATTGGAGACGACCAACGCT
GCTGCTGAGGCGGCGCGGGTGTTCGACGAAATCAAGCCGGACGGCATCTTTTGTTTCAACGATGTGCGCGCCACTTTCAT
CTACGAAGCCGCACGCGAACGCAGAGTGCGCATCGGCGCTGACGTGTCGGTGATCAGCATCGATAACCAGCCGTTCATCA
CTAATGTGATCAAACCATCACTGACTACGCTGGAACTGCCCCATTATGAGATGGGTTTTGAAGCGGTTCGGCAACTTGTC
GCCATACTTGACGGCGACAAGGCCGTAGCTGATCTTGAGTCCGATCGTGGCATGGCGGTGCAGAGGCAACGTGATGGCAG
CAAAACAGCATTGCGCTGCCGCATCATCGAACGCGATTCCATCGTGTTGTGA

Upstream 100 bases:

>100_bases
CCGTCTGCCTGGAATGGACATTCATGACCGCATGGAACAGAGCGCGGGCCACGCCCGAAACCAGACATCGTGACATGTTC
GCAAAAAGGGGGGTGATAAA

Downstream 100 bases:

>100_bases
CGGGTGTTCCCCTTTTTCATATTTAGTTTTCCGATTATCAGTTTTTCAATGCGTAGCCACGTGATATCTGATCATGTGGC
GCTGTGGAGGAAATTATTAT

Product: periplasmic binding protein/LacI transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MVQSSVSQRVNRSKRLVSIKDIAKEAGVSVCTVSLVMNNKDHNRVSKAVAQKIRATAKNMGYKPNSVARTLRTSKSNTLG
FVTDEIATTPYASKVLEGAQDAASKLGYILLTTNTNNDAELENQEISALLRYNVDGFLYAMMFHRKVDIPEVLEGSPTVV
VDSEDAAGRVPSVYPDDELAGYDATDCLIKAGCRRIVYYGSATPIVAQGQRLTGYRRALAEAGIPFDDALVVNVLETTNA
AAEAARVFDEIKPDGIFCFNDVRATFIYEAARERRVRIGADVSVISIDNQPFITNVIKPSLTTLELPHYEMGFEAVRQLV
AILDGDKAVADLESDRGMAVQRQRDGSKTALRCRIIERDSIVL

Sequences:

>Translated_363_residues
MVQSSVSQRVNRSKRLVSIKDIAKEAGVSVCTVSLVMNNKDHNRVSKAVAQKIRATAKNMGYKPNSVARTLRTSKSNTLG
FVTDEIATTPYASKVLEGAQDAASKLGYILLTTNTNNDAELENQEISALLRYNVDGFLYAMMFHRKVDIPEVLEGSPTVV
VDSEDAAGRVPSVYPDDELAGYDATDCLIKAGCRRIVYYGSATPIVAQGQRLTGYRRALAEAGIPFDDALVVNVLETTNA
AAEAARVFDEIKPDGIFCFNDVRATFIYEAARERRVRIGADVSVISIDNQPFITNVIKPSLTTLELPHYEMGFEAVRQLV
AILDGDKAVADLESDRGMAVQRQRDGSKTALRCRIIERDSIVL
>Mature_363_residues
MVQSSVSQRVNRSKRLVSIKDIAKEAGVSVCTVSLVMNNKDHNRVSKAVAQKIRATAKNMGYKPNSVARTLRTSKSNTLG
FVTDEIATTPYASKVLEGAQDAASKLGYILLTTNTNNDAELENQEISALLRYNVDGFLYAMMFHRKVDIPEVLEGSPTVV
VDSEDAAGRVPSVYPDDELAGYDATDCLIKAGCRRIVYYGSATPIVAQGQRLTGYRRALAEAGIPFDDALVVNVLETTNA
AAEAARVFDEIKPDGIFCFNDVRATFIYEAARERRVRIGADVSVISIDNQPFITNVIKPSLTTLELPHYEMGFEAVRQLV
AILDGDKAVADLESDRGMAVQRQRDGSKTALRCRIIERDSIVL

Specific function: Involved in the regulation of amylase production [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=309, Percent_Identity=29.4498381877023, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1790369, Length=320, Percent_Identity=26.5625, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1789068, Length=318, Percent_Identity=28.9308176100629, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1789202, Length=302, Percent_Identity=28.476821192053, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1787906, Length=256, Percent_Identity=27.34375, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1787948, Length=355, Percent_Identity=27.3239436619718, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1787580, Length=324, Percent_Identity=25.6172839506173, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1788474, Length=299, Percent_Identity=26.4214046822742, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI48994940, Length=324, Percent_Identity=26.2345679012346, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1790715, Length=311, Percent_Identity=24.7588424437299, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786540, Length=314, Percent_Identity=24.203821656051, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786268, Length=232, Percent_Identity=25.8620689655172, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1790689, Length=315, Percent_Identity=25.0793650793651, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 39760; Mature: 39760

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQSSVSQRVNRSKRLVSIKDIAKEAGVSVCTVSLVMNNKDHNRVSKAVAQKIRATAKNM
CCCCHHHHHHHHHHHEEHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHC
GYKPNSVARTLRTSKSNTLGFVTDEIATTPYASKVLEGAQDAASKLGYILLTTNTNNDAE
CCCCHHHHHHHHHCCCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCC
LENQEISALLRYNVDGFLYAMMFHRKVDIPEVLEGSPTVVVDSEDAAGRVPSVYPDDELA
CCHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHCCCCEEEEECCCCCCCCCCCCCCCCCC
GYDATDCLIKAGCRRIVYYGSATPIVAQGQRLTGYRRALAEAGIPFDDALVVNVLETTNA
CCCHHHHHHHHCCCEEEEECCCCCEEECCCHHHHHHHHHHHCCCCCCCHHEEEEHHHCCH
AAEAARVFDEIKPDGIFCFNDVRATFIYEAARERRVRIGADVSVISIDNQPFITNVIKPS
HHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHCEEEECCCEEEEEECCCCHHHHHCCCC
LTTLELPHYEMGFEAVRQLVAILDGDKAVADLESDRGMAVQRQRDGSKTALRCRIIERDS
CEEEECCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCEEEECCCCCCEEEEEEEECCCC
IVL
CCC
>Mature Secondary Structure
MVQSSVSQRVNRSKRLVSIKDIAKEAGVSVCTVSLVMNNKDHNRVSKAVAQKIRATAKNM
CCCCHHHHHHHHHHHEEHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHC
GYKPNSVARTLRTSKSNTLGFVTDEIATTPYASKVLEGAQDAASKLGYILLTTNTNNDAE
CCCCHHHHHHHHHCCCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCC
LENQEISALLRYNVDGFLYAMMFHRKVDIPEVLEGSPTVVVDSEDAAGRVPSVYPDDELA
CCHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHCCCCEEEEECCCCCCCCCCCCCCCCCC
GYDATDCLIKAGCRRIVYYGSATPIVAQGQRLTGYRRALAEAGIPFDDALVVNVLETTNA
CCCHHHHHHHHCCCEEEEECCCCCEEECCCHHHHHHHHHHHCCCCCCCHHEEEEHHHCCH
AAEAARVFDEIKPDGIFCFNDVRATFIYEAARERRVRIGADVSVISIDNQPFITNVIKPS
HHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHCEEEECCCEEEEEECCCCHHHHHCCCC
LTTLELPHYEMGFEAVRQLVAILDGDKAVADLESDRGMAVQRQRDGSKTALRCRIIERDS
CEEEECCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCEEEECCCCCCEEEEEEEECCCC
IVL
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7539689 [H]