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The map label for this gene is sacA [H]

Identifier: 213692912

GI number: 213692912

Start: 2266135

End: 2268045

Strand: Reverse

Name: sacA [H]

Synonym: Blon_2056

Alternate gene names: 213692912

Gene position: 2268045-2266135 (Counterclockwise)

Preceding gene: 213692913

Following gene: 213692911

Centisome position: 80.07

GC content: 57.4

Gene sequence:

>1911_bases
ATGCCTGACAACTATTCCGTTGCCATCGAAACCGGCGGCTACCGCTTGCTGGAATTGTTCGCCGAAAGATGCGGCGATGA
CGCTGCCGTCACCATTACAGACGGGGACGGCCATCAAATCGCCAGCCATGTCATGCCGGTGGCCGAGCGTCGCCACCGTT
TCTTGATTCCGGTGCCGACAAACGTGTGTCTCACGGTGAGAGCGCGCCAACTGACCGTGCGGTTCGCCTACTTGTCCGAG
TGTGAGAATCTTCTTGATGAAGGCGTGCGTTTCATCAGCATGAATCCATATGACAATGAATGGGATACGCAGCCGACGCT
GGATCAGATCTATGATCGTTTCGCTCGCCCTGCGGCGCATTTCGAGCCATTCGCTCGCTGGATGAATGATCCTAACGGCC
TGTGTCGTTTCCAAGGACGCTACCATCTGTTCTACCAATTCAATCCGTATGGTTTTGGCTGGGACAATATGCACTGGGGC
CATGCAGTGAGCCGCGATCTGGTGCATTGGACGCATCTGCCAATCTTCCTCGAACCGCAGCCGGAGCTGCATATCGACGA
ACGCATCGTTGGCGGCGCGTTCTCCGGATCCGCGGTGACGGTGGATGCGTGTGACAATCCCTGCAAGGGTGATGATGCGG
CCGCTATTCGCCTGTATCTAACCCGGCACCTGGAGACTCGCGGAGACGAATCGTCTGTTACTGAATACCAGACCACCTGC
TTGTGTGAGGATGGCGTGCATGTGAGTGTCGAATCTCCAGTCGCGTTGCGCGTTAACGATGGTTTTGGCTTCGATTTCCG
TGATCCGAAGGTGGAAACCGGCATGGGCGGCGAGGCGATCGATCCGGAACGCGCTTACATGGTGACCGCCACGAATCTGC
CGGTCGCCGAATTCGATACTGATGCTGTGTCCTCCGCGGCGCCTGGCATCAGCGTACAGGGTACAGACGGCTGGTTTACG
TGCGGTCCGCAAGGCATGCCCGGCACCGACAAGCCCAATATGGATACGGTGCCTGCCATGGCGCTCTTCTCCGCGAAGAA
ACCGCTCAAGCGCAACGTGACGTGGCAGTATGAAGGACCGGTGCTCGCCGACTTCGGCCACCAGCTGTCACGTACCTACG
AATGTCCTGACCTGTTCCAGCTCGACGGCAAGACGGTCGCCATCGGAGCTTTGATGCACTACCGTGACAAGCAAGGTCGT
TTCCAGCAGGTGCGTTGGTATGTTGGTGATCTCAAGGACACCGCCGATGGCCCACGTTTGCAGGTAGGGAACAGCGATTG
GTGCGATTTCGGTACCGGCTACTACGCGACACAAAGCTTTGCTGCCGACGACGGGCGTCGTATCGTGTTCGGCTGGTATA
CCGACTTTCCCGCGATGCGTGTGGAAAAGCCGTGCATCGCTAACGGCATGATGTCGCTGCCACGTGAACTGCACGTGCGT
GACGGCCGCTTGACCAGCCGTCCTGTCAAGGAAGTGTACGAGCAGCTGCTAGGCGATCGTTTGGAAGCGCATGCAGACGA
AAACGGGACCTTCTTTATTGTGCCGAACAACGCGTATTACACGGACATGCATCTGGCTGACGATGACGATTTCACAATGC
TCATCGCGACTGGTACCAATTCCGCCAATGGTAATTCGATGGAATTGCAGCTGCAGCGTCGCGACGGCGTGACCCGACTG
GTCACGAAGGGAACAGTCGTGGACGACATCGATTTCGATAGCGGCATCGCCGATGTGCGTCGCGTCGAAATCTTCTTCGA
CCGTAATGTGGTGGAAGTCTTCCTTAACAACGGCGAAGCGGCCGGCAGTATGCTGTTCCAAGGTGCGGACGGCGATGGCG
AGTTCCGTTTTGTTGCTGACGGCAAGGTCGATTGCGTGGATGTGCGTGTGCTTGACGGCATCTGGCGGTGA

Upstream 100 bases:

>100_bases
GACGGGTGTTCCCCTTTTTCATATTTAGTTTTCCGATTATCAGTTTTTCAATGCGTAGCCACGTGATATCTGATCATGTG
GCGCTGTGGAGGAAATTATT

Downstream 100 bases:

>100_bases
CATACAGCTGCTGCAGCGGTAGGTGGTAACCGGCAGTCATCTCAGTATGTAAGGCAAAACAGACGAAAGGCACGTGTTTT
GGCACGTGCCTTCCCTATAC

Product: glycosyl hydrolase family 32, N terminal domain protein

Products: NA

Alternate protein names: Sucrase; Invertase [H]

Number of amino acids: Translated: 636; Mature: 635

Protein sequence:

>636_residues
MPDNYSVAIETGGYRLLELFAERCGDDAAVTITDGDGHQIASHVMPVAERRHRFLIPVPTNVCLTVRARQLTVRFAYLSE
CENLLDEGVRFISMNPYDNEWDTQPTLDQIYDRFARPAAHFEPFARWMNDPNGLCRFQGRYHLFYQFNPYGFGWDNMHWG
HAVSRDLVHWTHLPIFLEPQPELHIDERIVGGAFSGSAVTVDACDNPCKGDDAAAIRLYLTRHLETRGDESSVTEYQTTC
LCEDGVHVSVESPVALRVNDGFGFDFRDPKVETGMGGEAIDPERAYMVTATNLPVAEFDTDAVSSAAPGISVQGTDGWFT
CGPQGMPGTDKPNMDTVPAMALFSAKKPLKRNVTWQYEGPVLADFGHQLSRTYECPDLFQLDGKTVAIGALMHYRDKQGR
FQQVRWYVGDLKDTADGPRLQVGNSDWCDFGTGYYATQSFAADDGRRIVFGWYTDFPAMRVEKPCIANGMMSLPRELHVR
DGRLTSRPVKEVYEQLLGDRLEAHADENGTFFIVPNNAYYTDMHLADDDDFTMLIATGTNSANGNSMELQLQRRDGVTRL
VTKGTVVDDIDFDSGIADVRRVEIFFDRNVVEVFLNNGEAAGSMLFQGADGDGEFRFVADGKVDCVDVRVLDGIWR

Sequences:

>Translated_636_residues
MPDNYSVAIETGGYRLLELFAERCGDDAAVTITDGDGHQIASHVMPVAERRHRFLIPVPTNVCLTVRARQLTVRFAYLSE
CENLLDEGVRFISMNPYDNEWDTQPTLDQIYDRFARPAAHFEPFARWMNDPNGLCRFQGRYHLFYQFNPYGFGWDNMHWG
HAVSRDLVHWTHLPIFLEPQPELHIDERIVGGAFSGSAVTVDACDNPCKGDDAAAIRLYLTRHLETRGDESSVTEYQTTC
LCEDGVHVSVESPVALRVNDGFGFDFRDPKVETGMGGEAIDPERAYMVTATNLPVAEFDTDAVSSAAPGISVQGTDGWFT
CGPQGMPGTDKPNMDTVPAMALFSAKKPLKRNVTWQYEGPVLADFGHQLSRTYECPDLFQLDGKTVAIGALMHYRDKQGR
FQQVRWYVGDLKDTADGPRLQVGNSDWCDFGTGYYATQSFAADDGRRIVFGWYTDFPAMRVEKPCIANGMMSLPRELHVR
DGRLTSRPVKEVYEQLLGDRLEAHADENGTFFIVPNNAYYTDMHLADDDDFTMLIATGTNSANGNSMELQLQRRDGVTRL
VTKGTVVDDIDFDSGIADVRRVEIFFDRNVVEVFLNNGEAAGSMLFQGADGDGEFRFVADGKVDCVDVRVLDGIWR
>Mature_635_residues
PDNYSVAIETGGYRLLELFAERCGDDAAVTITDGDGHQIASHVMPVAERRHRFLIPVPTNVCLTVRARQLTVRFAYLSEC
ENLLDEGVRFISMNPYDNEWDTQPTLDQIYDRFARPAAHFEPFARWMNDPNGLCRFQGRYHLFYQFNPYGFGWDNMHWGH
AVSRDLVHWTHLPIFLEPQPELHIDERIVGGAFSGSAVTVDACDNPCKGDDAAAIRLYLTRHLETRGDESSVTEYQTTCL
CEDGVHVSVESPVALRVNDGFGFDFRDPKVETGMGGEAIDPERAYMVTATNLPVAEFDTDAVSSAAPGISVQGTDGWFTC
GPQGMPGTDKPNMDTVPAMALFSAKKPLKRNVTWQYEGPVLADFGHQLSRTYECPDLFQLDGKTVAIGALMHYRDKQGRF
QQVRWYVGDLKDTADGPRLQVGNSDWCDFGTGYYATQSFAADDGRRIVFGWYTDFPAMRVEKPCIANGMMSLPRELHVRD
GRLTSRPVKEVYEQLLGDRLEAHADENGTFFIVPNNAYYTDMHLADDDDFTMLIATGTNSANGNSMELQLQRRDGVTRLV
TKGTVVDDIDFDSGIADVRRVEIFFDRNVVEVFLNNGEAAGSMLFQGADGDGEFRFVADGKVDCVDVRVLDGIWR

Specific function: Unknown

COG id: COG1621

COG function: function code G; Beta-fructosidases (levanase/invertase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 32 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322029, Length=181, Percent_Identity=33.7016574585635, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008985
- InterPro:   IPR001362
- InterPro:   IPR018053
- InterPro:   IPR013189
- InterPro:   IPR013148
- InterPro:   IPR006232 [H]

Pfam domain/function: PF08244 Glyco_hydro_32C; PF00251 Glyco_hydro_32N [H]

EC number: =3.2.1.26 [H]

Molecular weight: Translated: 71230; Mature: 71099

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS00609 GLYCOSYL_HYDROL_F32

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDNYSVAIETGGYRLLELFAERCGDDAAVTITDGDGHQIASHVMPVAERRHRFLIPVPT
CCCCEEEEEECCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEECCC
NVCLTVRARQLTVRFAYLSECENLLDEGVRFISMNPYDNEWDTQPTLDQIYDRFARPAAH
CEEEEEEEEEEEEEHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHCCCHHH
FEPFARWMNDPNGLCRFQGRYHLFYQFNPYGFGWDNMHWGHAVSRDLVHWTHLPIFLEPQ
HHHHHHHHCCCCCEEEECCCEEEEEEECCCCCCCCCCCCCHHHHHHCEEEECCCEEECCC
PELHIDERIVGGAFSGSAVTVDACDNPCKGDDAAAIRLYLTRHLETRGDESSVTEYQTTC
CCCEECCEEECCCCCCCEEEEECCCCCCCCCCCCEEEEEEEHHHHCCCCCCCCCEEEEEE
LCEDGVHVSVESPVALRVNDGFGFDFRDPKVETGMGGEAIDPERAYMVTATNLPVAEFDT
EECCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEECCC
DAVSSAAPGISVQGTDGWFTCGPQGMPGTDKPNMDTVPAMALFSAKKPLKRNVTWQYEGP
HHHHHCCCCCEEECCCCEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCHHCCCEEEECCC
VLADFGHQLSRTYECPDLFQLDGKTVAIGALMHYRDKQGRFQQVRWYVGDLKDTADGPRL
CHHHHHHHHHCCCCCCCCEEECCCEEEEEEHHHHCCCCCCCEEEHEEECCCCCCCCCCEE
QVGNSDWCDFGTGYYATQSFAADDGRRIVFGWYTDFPAMRVEKPCIANGMMSLPRELHVR
EECCCCCCCCCCCEEEECCCCCCCCCEEEEEEECCCCCCEECCCHHHHHHHHCCHHEEEC
DGRLTSRPVKEVYEQLLGDRLEAHADENGTFFIVPNNAYYTDMHLADDDDFTMLIATGTN
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEEECCCCCEEEEEEECCC
SANGNSMELQLQRRDGVTRLVTKGTVVDDIDFDSGIADVRRVEIFFDRNVVEVFLNNGEA
CCCCCEEEEEEECCCCCEEEEECCCEEECCCCCCCCCCEEEEEEEECCCEEEEEECCCCC
AGSMLFQGADGDGEFRFVADGKVDCVDVRVLDGIWR
CCEEEEECCCCCCCEEEEECCCEEEEEEEECCCCCC
>Mature Secondary Structure 
PDNYSVAIETGGYRLLELFAERCGDDAAVTITDGDGHQIASHVMPVAERRHRFLIPVPT
CCCEEEEEECCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEECCC
NVCLTVRARQLTVRFAYLSECENLLDEGVRFISMNPYDNEWDTQPTLDQIYDRFARPAAH
CEEEEEEEEEEEEEHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHCCCHHH
FEPFARWMNDPNGLCRFQGRYHLFYQFNPYGFGWDNMHWGHAVSRDLVHWTHLPIFLEPQ
HHHHHHHHCCCCCEEEECCCEEEEEEECCCCCCCCCCCCCHHHHHHCEEEECCCEEECCC
PELHIDERIVGGAFSGSAVTVDACDNPCKGDDAAAIRLYLTRHLETRGDESSVTEYQTTC
CCCEECCEEECCCCCCCEEEEECCCCCCCCCCCCEEEEEEEHHHHCCCCCCCCCEEEEEE
LCEDGVHVSVESPVALRVNDGFGFDFRDPKVETGMGGEAIDPERAYMVTATNLPVAEFDT
EECCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEECCC
DAVSSAAPGISVQGTDGWFTCGPQGMPGTDKPNMDTVPAMALFSAKKPLKRNVTWQYEGP
HHHHHCCCCCEEECCCCEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCHHCCCEEEECCC
VLADFGHQLSRTYECPDLFQLDGKTVAIGALMHYRDKQGRFQQVRWYVGDLKDTADGPRL
CHHHHHHHHHCCCCCCCCEEECCCEEEEEEHHHHCCCCCCCEEEHEEECCCCCCCCCCEE
QVGNSDWCDFGTGYYATQSFAADDGRRIVFGWYTDFPAMRVEKPCIANGMMSLPRELHVR
EECCCCCCCCCCCEEEECCCCCCCCCEEEEEEECCCCCCEECCCHHHHHHHHCCHHEEEC
DGRLTSRPVKEVYEQLLGDRLEAHADENGTFFIVPNNAYYTDMHLADDDDFTMLIATGTN
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEEECCCCCEEEEEEECCC
SANGNSMELQLQRRDGVTRLVTKGTVVDDIDFDSGIADVRRVEIFFDRNVVEVFLNNGEA
CCCCCEEEEEEECCCCCEEEEECCCEEECCCCCCCCCCEEEEEEEECCCEEEEEECCCCC
AGSMLFQGADGDGEFRFVADGKVDCVDVRVLDGIWR
CCEEEEECCCCCCCEEEEECCCEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2254250; 1368686 [H]