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The map label for this gene is glnH [H]

Identifier: 213692880

GI number: 213692880

Start: 2208779

End: 2209726

Strand: Reverse

Name: glnH [H]

Synonym: Blon_2021

Alternate gene names: 213692880

Gene position: 2209726-2208779 (Counterclockwise)

Preceding gene: 213692881

Following gene: 213692879

Centisome position: 78.01

GC content: 61.29

Gene sequence:

>948_bases
ATGCAGTCCACCAAACTGAAGTCCATTATGGCCATTGCACTGAGCTCGGCCATGCTGTTCGCCACCGCCGCTTGCGGCAC
CTCCGACAAGGCCGATGCCGGTACGGATTCCGCCAAGGGCGGCTCCTCCAAGTCGGTCACCATCACCAGCTACGACGTGA
GCTCCGTGAAGAAGGACGATGCAATAGCCGCACTGCTGCCTGAATCCGTGACCAAGGACGGCAAGCTCACCATCGGCACC
AACCCCTCCTACGCCCCGGCCGAATTCCTCGACGCCGACGGCAAGACCCAGATCGGCTACGACATGGATCTCGCCCGCGC
CATGGGCAACATCTTCGGTCTCGAGACCGAAATCGTCTCCTCCAACTTCGATACGATCATTCCGGCCATCGGCTCCAAGT
ACGATCTGGGCATCGCCGCCTTCACCATCACCAAGGAGCGCATGGAATCCGTCGACTTCGTCTCCTACTTCACCGCAGGC
ATGGGCTATGCAGTGGCCGCAGGCAACCCCAAGAATGTCGACGAGAACGACCTGTGCGGTCTGAACGTTGCCGTCGAGAC
CGGCACCGTCGAGGAAGAGGCCATCAACAAGACCGCCAAGCAGTGCAAGGCGGACGGCAAGAAAGACATCACCATCCAGT
CCTCCAAGCAGCAGACCGACGCCACCACCGCTGTGGTGACCGGCAAGGCCGACGTGTTCTTCGCCGATTCCCCGGTTGTC
GGCTACGCCATCGCCCAGACCGAAGGCCAGCTGGAACAGCTCGGCAAGGACTTCGACTCCGTGCCGAACGCCATCGCCAT
CAAGAAGGGCGATTCCCAGACCACCGAAGCCGTGCAGAAGGCCATGCAGAAGCTCATGGACGACGGCACCTACACCAAGA
TCCTCCAGCACTGGGGCGTCGAATCCGGCGCACTGGACAAGGCCGAAATCAACCCGGCCGTCGAATAG

Upstream 100 bases:

>100_bases
GGCGGGAGTCTTTTTGTATATGTATGCACTGTAGTGTATAAATATGCTTAACCGTTGATCGTTGCCGTTGAAGCGGCGTA
AGAGAGAAGAAGAAACGATT

Downstream 100 bases:

>100_bases
TCCCCCCCAAGAATGCTCCCGCTTGCGGGAGCTGGCTCGCGGAGCGAGACTGAGGGTGGTCCACACGACGCCCAGCAATC
CAGATGGGGCGCACTGTTTG

Product: extracellular solute-binding protein, family 3

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: GlnBP [H]

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MQSTKLKSIMAIALSSAMLFATAACGTSDKADAGTDSAKGGSSKSVTITSYDVSSVKKDDAIAALLPESVTKDGKLTIGT
NPSYAPAEFLDADGKTQIGYDMDLARAMGNIFGLETEIVSSNFDTIIPAIGSKYDLGIAAFTITKERMESVDFVSYFTAG
MGYAVAAGNPKNVDENDLCGLNVAVETGTVEEEAINKTAKQCKADGKKDITIQSSKQQTDATTAVVTGKADVFFADSPVV
GYAIAQTEGQLEQLGKDFDSVPNAIAIKKGDSQTTEAVQKAMQKLMDDGTYTKILQHWGVESGALDKAEINPAVE

Sequences:

>Translated_315_residues
MQSTKLKSIMAIALSSAMLFATAACGTSDKADAGTDSAKGGSSKSVTITSYDVSSVKKDDAIAALLPESVTKDGKLTIGT
NPSYAPAEFLDADGKTQIGYDMDLARAMGNIFGLETEIVSSNFDTIIPAIGSKYDLGIAAFTITKERMESVDFVSYFTAG
MGYAVAAGNPKNVDENDLCGLNVAVETGTVEEEAINKTAKQCKADGKKDITIQSSKQQTDATTAVVTGKADVFFADSPVV
GYAIAQTEGQLEQLGKDFDSVPNAIAIKKGDSQTTEAVQKAMQKLMDDGTYTKILQHWGVESGALDKAEINPAVE
>Mature_315_residues
MQSTKLKSIMAIALSSAMLFATAACGTSDKADAGTDSAKGGSSKSVTITSYDVSSVKKDDAIAALLPESVTKDGKLTIGT
NPSYAPAEFLDADGKTQIGYDMDLARAMGNIFGLETEIVSSNFDTIIPAIGSKYDLGIAAFTITKERMESVDFVSYFTAG
MGYAVAAGNPKNVDENDLCGLNVAVETGTVEEEAINKTAKQCKADGKKDITIQSSKQQTDATTAVVTGKADVFFADSPVV
GYAIAQTEGQLEQLGKDFDSVPNAIAIKKGDSQTTEAVQKAMQKLMDDGTYTKILQHWGVESGALDKAEINPAVE

Specific function: Involved in a glutamine-transport system glnHPQ [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787031, Length=230, Percent_Identity=31.304347826087, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788649, Length=250, Percent_Identity=29.2, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788648, Length=241, Percent_Identity=27.8008298755187, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788228, Length=236, Percent_Identity=24.1525423728814, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787088, Length=245, Percent_Identity=26.1224489795918, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787085, Length=233, Percent_Identity=25.3218884120172, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: 1440 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 460 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 33055; Mature: 33055

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00146 BETA_LACTAMASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSTKLKSIMAIALSSAMLFATAACGTSDKADAGTDSAKGGSSKSVTITSYDVSSVKKDD
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHCCCCC
AIAALLPESVTKDGKLTIGTNPSYAPAEFLDADGKTQIGYDMDLARAMGNIFGLETEIVS
HHHHHCCHHHCCCCCEEECCCCCCCCHHHHCCCCCEECCCCHHHHHHHHHHHCCHHHHHH
SNFDTIIPAIGSKYDLGIAAFTITKERMESVDFVSYFTAGMGYAVAAGNPKNVDENDLCG
CCCCHHHHCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCC
LNVAVETGTVEEEAINKTAKQCKADGKKDITIQSSKQQTDATTAVVTGKADVFFADSPVV
EEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEEEEECCCEEEECCCCCE
GYAIAQTEGQLEQLGKDFDSVPNAIAIKKGDSQTTEAVQKAMQKLMDDGTYTKILQHWGV
EEEEECCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCC
ESGALDKAEINPAVE
CCCCCCHHCCCCCCC
>Mature Secondary Structure
MQSTKLKSIMAIALSSAMLFATAACGTSDKADAGTDSAKGGSSKSVTITSYDVSSVKKDD
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHCCCCC
AIAALLPESVTKDGKLTIGTNPSYAPAEFLDADGKTQIGYDMDLARAMGNIFGLETEIVS
HHHHHCCHHHCCCCCEEECCCCCCCCHHHHCCCCCEECCCCHHHHHHHHHHHCCHHHHHH
SNFDTIIPAIGSKYDLGIAAFTITKERMESVDFVSYFTAGMGYAVAAGNPKNVDENDLCG
CCCCHHHHCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCC
LNVAVETGTVEEEAINKTAKQCKADGKKDITIQSSKQQTDATTAVVTGKADVFFADSPVV
EEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEEEEECCCEEEECCCCCE
GYAIAQTEGQLEQLGKDFDSVPNAIAIKKGDSQTTEAVQKAMQKLMDDGTYTKILQHWGV
EEEEECCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCC
ESGALDKAEINPAVE
CCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: Translocates substrate across the membrane [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]