Click here to switch to the map view.

The map label for this gene is pdxT [H]

Identifier: 213692852

GI number: 213692852

Start: 2173343

End: 2173981

Strand: Reverse

Name: pdxT [H]

Synonym: Blon_1993

Alternate gene names: 213692852

Gene position: 2173981-2173343 (Counterclockwise)

Preceding gene: 213692853

Following gene: 213692851

Centisome position: 76.74

GC content: 59.62

Gene sequence:

>639_bases
ATGGTTGTAGCTGCTGAATATATTTCCAAAGAAGAGTCCGAGGACGCCGAAAACGCCAAGCACGGCGTGACCGGCATCCT
GGCCGTACAAGGCGCATTCGCCGAACATGCGGCGGTGCTGGACAAGCTCGGTGCGCCGTGGAAACTGCTGCGCGCGACCG
AGGATTTCGATGAATCCATCGACCGCGTGATTCTACCCGGCGGCGAATCCACTACACAGGGCAAGCTCCTGCATTCGACC
GGACTGTTCGAGTCGATCGCCGTACACATCAAGGCAGGAAAACCGGTATTCGGCACTTGCGCCGGCATGATTCTGCTGGC
TAAAAAGCTCGACAATGACGACAATGTCTACTTTGGCGCGCTCGACGCCGTCGTACGCCGCAACGCCTATGGTCGTCAGC
TCGGTAGTTTCCAGGCCACCGCCGATTTTGGTGCGGCGGATGATCCGCAGCGTATCACGGACTTCCCACTGGTATTCATC
CGCGGACCGTACGTGGTGTCGGTCGGCCCTGAGGCCACGGTCGAAACCGAAGTCGATGGCCACGTGGTGGGCTTGCGTCA
AGGCAATATCCTGGCCACCGCCTTCCACCCGGAACTCACGGACGATACCCGCATCCACGAGCTCTTCCTGTCGCTGTAG

Upstream 100 bases:

>100_bases
GCTCGCGGCGAGTGAGTGCATCACCTTGGCTCCCCTCTTTGAGGGGAGCCAAGAAAAGGTGACGCTCCCGCCAGCGGGAG
TCAAGGCCCAAGGAGACAAC

Downstream 100 bases:

>100_bases
TCGATTCGTCTGCCGCTAGACTGGTATCCGACTGACGAATCAAGGAGACGATCTGCCATGCAGCTTGAGCTGAATCGACG
CAGCAAGGTGCCGATGTACC

Product: glutamine amidotransferase subunit PdxT

Products: NA

Alternate protein names: Glutamine amidotransferase glutaminase subunit pdxT [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MVVAAEYISKEESEDAENAKHGVTGILAVQGAFAEHAAVLDKLGAPWKLLRATEDFDESIDRVILPGGESTTQGKLLHST
GLFESIAVHIKAGKPVFGTCAGMILLAKKLDNDDNVYFGALDAVVRRNAYGRQLGSFQATADFGAADDPQRITDFPLVFI
RGPYVVSVGPEATVETEVDGHVVGLRQGNILATAFHPELTDDTRIHELFLSL

Sequences:

>Translated_212_residues
MVVAAEYISKEESEDAENAKHGVTGILAVQGAFAEHAAVLDKLGAPWKLLRATEDFDESIDRVILPGGESTTQGKLLHST
GLFESIAVHIKAGKPVFGTCAGMILLAKKLDNDDNVYFGALDAVVRRNAYGRQLGSFQATADFGAADDPQRITDFPLVFI
RGPYVVSVGPEATVETEVDGHVVGLRQGNILATAFHPELTDDTRIHELFLSL
>Mature_212_residues
MVVAAEYISKEESEDAENAKHGVTGILAVQGAFAEHAAVLDKLGAPWKLLRATEDFDESIDRVILPGGESTTQGKLLHST
GLFESIAVHIKAGKPVFGTCAGMILLAKKLDNDDNVYFGALDAVVRRNAYGRQLGSFQATADFGAADDPQRITDFPLVFI
RGPYVVSVGPEATVETEVDGHVVGLRQGNILATAFHPELTDDTRIHELFLSL

Specific function: Involved in the hydrolysis of glutamine to glutamate and ammonia. Channels an ammonia molecule to pdxS [H]

COG id: COG0311

COG function: function code H; Predicted glutamine amidotransferase involved in pyridoxine biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine amidotransferase pdxT/SNO family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6323742, Length=208, Percent_Identity=37.5, Blast_Score=114, Evalue=9e-27,
Organism=Saccharomyces cerevisiae, GI6323995, Length=217, Percent_Identity=33.6405529953917, Blast_Score=105, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6321048, Length=217, Percent_Identity=33.1797235023041, Blast_Score=102, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002161
- InterPro:   IPR021196 [H]

Pfam domain/function: PF01174 SNO [H]

EC number: NA

Molecular weight: Translated: 22776; Mature: 22776

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS51130 PDXT_SNO_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVAAEYISKEESEDAENAKHGVTGILAVQGAFAEHAAVLDKLGAPWKLLRATEDFDESI
CEEEHHHHCCCCCCHHHHHHCCCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHCC
DRVILPGGESTTQGKLLHSTGLFESIAVHIKAGKPVFGTCAGMILLAKKLDNDDNVYFGA
CEEEECCCCCCCCCEEEECCCCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEHH
LDAVVRRNAYGRQLGSFQATADFGAADDPQRITDFPLVFIRGPYVVSVGPEATVETEVDG
HHHHHHCCHHHHHHCCEEECCCCCCCCCCCCCCCCCEEEEECCEEEEECCCCEEEECCCC
HVVGLRQGNILATAFHPELTDDTRIHELFLSL
EEEEEECCCEEEEEECCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MVVAAEYISKEESEDAENAKHGVTGILAVQGAFAEHAAVLDKLGAPWKLLRATEDFDESI
CEEEHHHHCCCCCCHHHHHHCCCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHCC
DRVILPGGESTTQGKLLHSTGLFESIAVHIKAGKPVFGTCAGMILLAKKLDNDDNVYFGA
CEEEECCCCCCCCCEEEECCCCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEHH
LDAVVRRNAYGRQLGSFQATADFGAADDPQRITDFPLVFIRGPYVVSVGPEATVETEVDG
HHHHHHCCHHHHHHCCEEECCCCCCCCCCCCCCCCCEEEEECCEEEEECCCCEEEECCCC
HVVGLRQGNILATAFHPELTDDTRIHELFLSL
EEEEEECCCEEEEEECCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12381787 [H]