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The map label for this gene is purT [H]

Identifier: 213692800

GI number: 213692800

Start: 2117394

End: 2118731

Strand: Direct

Name: purT [H]

Synonym: Blon_1938

Alternate gene names: 213692800

Gene position: 2117394-2118731 (Clockwise)

Preceding gene: 213692797

Following gene: 213692802

Centisome position: 74.75

GC content: 66.29

Gene sequence:

>1338_bases
ATGACTGAAAACGCCGTGAATACTGCCGTGACTTCCGCCTCCTCTCCCGCAATTCCCGCCGAGACTTCCGCCGTCCCCCC
CGCAACTCGCACAGCCAACCGCCCGCTGGGCACACCGCTGGGCAAGCACCCCACCCGCGTGCTGTTTTTGGGCGCTGGCG
AGCTGGGCAAGGAGGTCACACTTGAACTCATGCGACTGGGCGCATGGGTGTGTGCTGCTGACTCCTATGCCGGCGCTCCG
GCCCAGCAGGTGGCGCATGAATACCGCGCGTTGGATATGGCGAATGCCGCCGAATTGCAGGCATTGTTCGATGAAATCAA
GCCGGACATCATCGTGCCCGAGGTAGAGGCCATCGCCACCGATGTGCTGGCCGGTGCTGCGGCCGCGGGCGCCCAGGTGG
TGCCGAGCGCCGAAATCGCCGCGATTTGCATGGATCGGGAGCGTCTGCGCGTACTGGCCCACGAAGAATTGGGCTTGCCG
ACCACACCGTATCGTTTTGCCGGCTCGCTGGAAGAGCTGCACGCCGGAGCTTCGGAAGTCGGCTATCCGTGCGTGGTCAA
GCCGGTGATGAGCTCGTCTGGTCACGGCCAGTCGGTGGTGCGTTCCGCCGACGCCATCGATGCCGCTTGGACCGAAGCGC
AGGAGGGCCGCCGCGCCGCCGATGAGGGCGATGTGTCGCGCGTGATCGTTGAGGCGCTTGCCCCGCTGGAGCGTGAGCTG
ACGGTACTGACCGTTTCTTCGTCCGCCGGCATTGTGACGTGCGCGCCGATCGGCCAACGTCAGGAGTCCGGCGATTACCG
CGAGTCCTGGCAGCCGGCTACCGAGCCCGATGGCGAGGCCGAGCGGGCGCGCGACATCGCACGTACCGCCGTTGAAGGCT
TGGTGGCCAAGGCTCAGGCGACCGGCGAAACCGGTTGGGGCGTATTCGGCGTGGAGCTGTTCGTGCTCACCGACGGCTCG
ATTCTGTTCAACGAGGTCTCCCCCCGTCCTCACGATACCGGTATGGTGACGATGACCTCCCAGCGTCTGAGCGAGTTCGC
GCTGCACGCCCGCGCGATTCTCGGCCTGCCGATCACCCCCGAACATGTGGCGCTCACGATTCCGGCCGGCTCGGTGGCGG
CAAGCCATGCGATTGTGGTGGCCGGCGACGGTGAGGCCGAGTTCACCGATGTGGCCGCTGCTCTGGCCGGACCCGGCACG
GACCTGCGCATTTTTGCCAAGCCCGAAGTGCATGGCCACCGTCGTATGGCCGTGGCACTGGCCGTCGGCGAGTCCGAAGC
CGACGCCCGTGCCAAGGCCGGCCGTGTTGCCGATGCGTTGACGATTACCGTGGAGTAG

Upstream 100 bases:

>100_bases
CCGCCCCATGAAGCCGGATATTCCGCCCGTAATTTTGGCCGCGCGAGGCACTCCGCTCACGCGACATACCGAAATCATCG
CGTCATGGCAGAATGTGGGT

Downstream 100 bases:

>100_bases
TCCACGGCCTGATCAGCTCAGCATGGCCTCAATATTATTGCCGTCCGGGTCGTGGATGAACGCGGCGTAATACGTGGGGC
CATAGTTCGGGCGCGGGCCC

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2 [H]

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MTENAVNTAVTSASSPAIPAETSAVPPATRTANRPLGTPLGKHPTRVLFLGAGELGKEVTLELMRLGAWVCAADSYAGAP
AQQVAHEYRALDMANAAELQALFDEIKPDIIVPEVEAIATDVLAGAAAAGAQVVPSAEIAAICMDRERLRVLAHEELGLP
TTPYRFAGSLEELHAGASEVGYPCVVKPVMSSSGHGQSVVRSADAIDAAWTEAQEGRRAADEGDVSRVIVEALAPLEREL
TVLTVSSSAGIVTCAPIGQRQESGDYRESWQPATEPDGEAERARDIARTAVEGLVAKAQATGETGWGVFGVELFVLTDGS
ILFNEVSPRPHDTGMVTMTSQRLSEFALHARAILGLPITPEHVALTIPAGSVAASHAIVVAGDGEAEFTDVAAALAGPGT
DLRIFAKPEVHGHRRMAVALAVGESEADARAKAGRVADALTITVE

Sequences:

>Translated_445_residues
MTENAVNTAVTSASSPAIPAETSAVPPATRTANRPLGTPLGKHPTRVLFLGAGELGKEVTLELMRLGAWVCAADSYAGAP
AQQVAHEYRALDMANAAELQALFDEIKPDIIVPEVEAIATDVLAGAAAAGAQVVPSAEIAAICMDRERLRVLAHEELGLP
TTPYRFAGSLEELHAGASEVGYPCVVKPVMSSSGHGQSVVRSADAIDAAWTEAQEGRRAADEGDVSRVIVEALAPLEREL
TVLTVSSSAGIVTCAPIGQRQESGDYRESWQPATEPDGEAERARDIARTAVEGLVAKAQATGETGWGVFGVELFVLTDGS
ILFNEVSPRPHDTGMVTMTSQRLSEFALHARAILGLPITPEHVALTIPAGSVAASHAIVVAGDGEAEFTDVAAALAGPGT
DLRIFAKPEVHGHRRMAVALAVGESEADARAKAGRVADALTITVE
>Mature_444_residues
TENAVNTAVTSASSPAIPAETSAVPPATRTANRPLGTPLGKHPTRVLFLGAGELGKEVTLELMRLGAWVCAADSYAGAPA
QQVAHEYRALDMANAAELQALFDEIKPDIIVPEVEAIATDVLAGAAAAGAQVVPSAEIAAICMDRERLRVLAHEELGLPT
TPYRFAGSLEELHAGASEVGYPCVVKPVMSSSGHGQSVVRSADAIDAAWTEAQEGRRAADEGDVSRVIVEALAPLERELT
VLTVSSSAGIVTCAPIGQRQESGDYRESWQPATEPDGEAERARDIARTAVEGLVAKAQATGETGWGVFGVELFVLTDGSI
LFNEVSPRPHDTGMVTMTSQRLSEFALHARAILGLPITPEHVALTIPAGSVAASHAIVVAGDGEAEFTDVAAALAGPGTD
LRIFAKPEVHGHRRMAVALAVGESEADARAKAGRVADALTITVE

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP [H]

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1788155, Length=405, Percent_Identity=48.641975308642, Blast_Score=337, Evalue=6e-94,
Organism=Saccharomyces cerevisiae, GI6324702, Length=413, Percent_Identity=25.4237288135593, Blast_Score=113, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: 2.1.2.- [C]

Molecular weight: Translated: 46257; Mature: 46126

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS50975 ATP_GRASP ; PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTENAVNTAVTSASSPAIPAETSAVPPATRTANRPLGTPLGKHPTRVLFLGAGELGKEVT
CCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHH
LELMRLGAWVCAADSYAGAPAQQVAHEYRALDMANAAELQALFDEIKPDIIVPEVEAIAT
HHHHHHHHHEEECCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEECCCHHHHHH
DVLAGAAAAGAQVVPSAEIAAICMDRERLRVLAHEELGLPTTPYRFAGSLEELHAGASEV
HHHHHHHHCCCCCCCCHHHHHHHHCHHHHHHHHHHHCCCCCCCHHHHCCHHHHHCCHHHC
GYPCVVKPVMSSSGHGQSVVRSADAIDAAWTEAQEGRRAADEGDVSRVIVEALAPLEREL
CCCEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCE
TVLTVSSSAGIVTCAPIGQRQESGDYRESWQPATEPDGEAERARDIARTAVEGLVAKAQA
EEEEEECCCCEEEECCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
TGETGWGVFGVELFVLTDGSILFNEVSPRPHDTGMVTMTSQRLSEFALHARAILGLPITP
CCCCCCEEEEEEEEEEECCCEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCC
EHVALTIPAGSVAASHAIVVAGDGEAEFTDVAAALAGPGTDLRIFAKPEVHGHRRMAVAL
CCEEEEECCCCHHCCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEE
AVGESEADARAKAGRVADALTITVE
EECCCCHHHHHHCCCCEEEEEEEEC
>Mature Secondary Structure 
TENAVNTAVTSASSPAIPAETSAVPPATRTANRPLGTPLGKHPTRVLFLGAGELGKEVT
CCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHH
LELMRLGAWVCAADSYAGAPAQQVAHEYRALDMANAAELQALFDEIKPDIIVPEVEAIAT
HHHHHHHHHEEECCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEECCCHHHHHH
DVLAGAAAAGAQVVPSAEIAAICMDRERLRVLAHEELGLPTTPYRFAGSLEELHAGASEV
HHHHHHHHCCCCCCCCHHHHHHHHCHHHHHHHHHHHCCCCCCCHHHHCCHHHHHCCHHHC
GYPCVVKPVMSSSGHGQSVVRSADAIDAAWTEAQEGRRAADEGDVSRVIVEALAPLEREL
CCCEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCE
TVLTVSSSAGIVTCAPIGQRQESGDYRESWQPATEPDGEAERARDIARTAVEGLVAKAQA
EEEEEECCCCEEEECCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
TGETGWGVFGVELFVLTDGSILFNEVSPRPHDTGMVTMTSQRLSEFALHARAILGLPITP
CCCCCCEEEEEEEEEEECCCEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCC
EHVALTIPAGSVAASHAIVVAGDGEAEFTDVAAALAGPGTDLRIFAKPEVHGHRRMAVAL
CCEEEEECCCCHHCCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEE
AVGESEADARAKAGRVADALTITVE
EECCCCHHHHHHCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12381787 [H]