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The map label for this gene is pyrI [H]

Identifier: 213692325

GI number: 213692325

Start: 1609025

End: 1609444

Strand: Reverse

Name: pyrI [H]

Synonym: Blon_1450

Alternate gene names: 213692325

Gene position: 1609444-1609025 (Counterclockwise)

Preceding gene: 213692326

Following gene: 213692324

Centisome position: 56.82

GC content: 57.86

Gene sequence:

>420_bases
ATGGAAGTCACCAGCATTCAGAACGGCATCATTATCGACCATGTGACGGCTGGAACCTCGCTGAAGGTTCTTGAATACCT
GAAAATCGACCCGGCCAAAACCAAGCTTGCGCTCATCATGAACGCCAACTCCAAGCGTTACGGCTCCAAGGACATCATCA
AAATCGAGGATGACAAGGACATCGACCTCGACGTGCTCGGCTTCGTGGCCCGCCAGGCCACCGTAGACGTGGTGCGCGGC
GGCAAGATCGTCGAAAAGAAGCAGCCGAACCTGCCCGAGCACATCGTCGGCGTGATCTCCTGCGTGAACCCGCGCTGCGT
GACCACCACCGAGCCCGGCATCAAGCAGATGTTCCACCTCGTGCACTCCGAACGGCTCGAATACCGCTGCGACTACTGCG
ACGAGGAAGCCAAACTGTGA

Upstream 100 bases:

>100_bases
TCAAGAACGGCATGCTCGTGCGCATGGCCCTCGAAAGCACCGTGGTGGGCGACGAACTGCCCGGATACGAACCGCTCAAC
TCCAAGGAGGTTCATGCCTG

Downstream 100 bases:

>100_bases
GCATCACCCTACGCAACATCAAGGTCTGGAACACCGGCGAAGTCATCGACCTCGTGGTGCCCGGGACCGCTGCCGCCTAC
TTCGCCGACACCTCCGCCGT

Product: aspartate carbamoyltransferase regulatory subunit

Products: phosphate; N-carbamoyl-L-aspartate

Alternate protein names: NA

Number of amino acids: Translated: 139; Mature: 139

Protein sequence:

>139_residues
MEVTSIQNGIIIDHVTAGTSLKVLEYLKIDPAKTKLALIMNANSKRYGSKDIIKIEDDKDIDLDVLGFVARQATVDVVRG
GKIVEKKQPNLPEHIVGVISCVNPRCVTTTEPGIKQMFHLVHSERLEYRCDYCDEEAKL

Sequences:

>Translated_139_residues
MEVTSIQNGIIIDHVTAGTSLKVLEYLKIDPAKTKLALIMNANSKRYGSKDIIKIEDDKDIDLDVLGFVARQATVDVVRG
GKIVEKKQPNLPEHIVGVISCVNPRCVTTTEPGIKQMFHLVHSERLEYRCDYCDEEAKL
>Mature_139_residues
MEVTSIQNGIIIDHVTAGTSLKVLEYLKIDPAKTKLALIMNANSKRYGSKDIIKIEDDKDIDLDVLGFVARQATVDVVRG
GKIVEKKQPNLPEHIVGVISCVNPRCVTTTEPGIKQMFHLVHSERLEYRCDYCDEEAKL

Specific function: Involved in allosteric regulation of aspartate carbamoyltransferase [H]

COG id: COG1781

COG function: function code F; Aspartate carbamoyltransferase, regulatory subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyrI family [H]

Homologues:

Organism=Escherichia coli, GI1790692, Length=138, Percent_Identity=31.1594202898551, Blast_Score=81, Evalue=3e-17,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 440 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020545
- InterPro:   IPR020542
- ProDom:   PD006194 [H]

Pfam domain/function: PF01948 PyrI; PF02748 PyrI_C [H]

EC number: 2.1.3.2

Molecular weight: Translated: 15551; Mature: 15551

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVTSIQNGIIIDHVTAGTSLKVLEYLKIDPAKTKLALIMNANSKRYGSKDIIKIEDDKD
CCCCCCCCCEEEEEECCCCCCHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCEEECCCCC
IDLDVLGFVARQATVDVVRGGKIVEKKQPNLPEHIVGVISCVNPRCVTTTEPGIKQMFHL
CCHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHCCCCEEEECCCHHHHHHHHH
VHSERLEYRCDYCDEEAKL
HHHHHHHEECCCCCCCCCC
>Mature Secondary Structure
MEVTSIQNGIIIDHVTAGTSLKVLEYLKIDPAKTKLALIMNANSKRYGSKDIIKIEDDKD
CCCCCCCCCEEEEEECCCCCCHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCEEECCCCC
IDLDVLGFVARQATVDVVRGGKIVEKKQPNLPEHIVGVISCVNPRCVTTTEPGIKQMFHL
CCHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHCCCCEEEECCCHHHHHHHHH
VHSERLEYRCDYCDEEAKL
HHHHHHHEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: carbamoyl phosphate; L-aspartate

Specific reaction: carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12381787 [H]