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The map label for this gene is pyrB [H]

Identifier: 213692326

GI number: 213692326

Start: 1609444

End: 1610406

Strand: Reverse

Name: pyrB [H]

Synonym: Blon_1451

Alternate gene names: 213692326

Gene position: 1610406-1609444 (Counterclockwise)

Preceding gene: 213692327

Following gene: 213692325

Centisome position: 56.85

GC content: 63.45

Gene sequence:

>963_bases
ATGGTCGGCAAAAGCGTCGTAACGCTGGACGGCCTGTCTACAAACCAGATTCTCGACCTGCTGCATAAGGCCGAATACAT
CGATTCCCACCGCAAAGAGGTTGCCCATACCTGCGATGGGCGAGTGCTGGCCACGCTGTTCTACGAGCCCTCCACCCGTA
CGCGCCTGAGCTTTGAGACCGCAATGCTGCGTCTCGGCGGCAAGGTCATCGGATTCGCCGGCGCGCAGCTGGCCTCCGTG
ACCAAGGGCGAATCCATCGCCGACACGTTGAAGACCGTGTCCAACTACGTGGACGTGGTCGCCATCCGTCACCCGAAGGA
GGGTGCCGCGCTGGTGGCCTCCCGCGCCGCATCCGTGCCGGTGATCAACGCCGGCGACGGCGGCCACATGCACCCCACGC
AGACGCTGGCCGACCTGGCCACGTTGCAGTCGCGCTTCGGCCGCATCACCGACCTGACCGTGGGACTGTGCGGCGATTTG
ACCTTCGGCCGCACTGTGCACTCCCTGATCGAGACCCTGTGCCGCTTCGGCAACGTGCGCTTCGTGCTCATCAGCCCGGA
CGAGCTCAAGACCCCGCAGTACGTGATCGACCGCATCAACGCCACAGACAACTGCTCCTACGTGGAAGTGCGCGACCTGG
CCTCCGTGATCGGCGACCTCGACGTGCTGTACATGACCCGCGTACAGAAGGAACGCTTCTTCAACGAAGACGACTACTTG
CGCCTGCGCGACACCTACATTCTCGACGAAGAGAAGCTGCAGCTCGCCAAGCCGTCCATGGCCGTGCTTCACCCGCTGCC
GCGCGTCAACGAAATCGCCGTGGACGTGGACGACGACCCGCGTGCCGCCTACTTCGAACAGGTCAAGAACGGCATGCTCG
TGCGCATGGCCCTCGAAAGCACCGTGGTGGGCGACGAACTGCCCGGATACGAACCGCTCAACTCCAAGGAGGTTCATGCC
TGA

Upstream 100 bases:

>100_bases
TGCGGCTGTCCGAAAAATGGCGTAAGTTATATTAGGTCACACAAGGGTGGCCACCTGAAAACCACGAGCGAAAAGGTGAG
CCTTGTCTGAAGAAACGCCA

Downstream 100 bases:

>100_bases
TGGAAGTCACCAGCATTCAGAACGGCATCATTATCGACCATGTGACGGCTGGAACCTCGCTGAAGGTTCTTGAATACCTG
AAAATCGACCCGGCCAAAAC

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MVGKSVVTLDGLSTNQILDLLHKAEYIDSHRKEVAHTCDGRVLATLFYEPSTRTRLSFETAMLRLGGKVIGFAGAQLASV
TKGESIADTLKTVSNYVDVVAIRHPKEGAALVASRAASVPVINAGDGGHMHPTQTLADLATLQSRFGRITDLTVGLCGDL
TFGRTVHSLIETLCRFGNVRFVLISPDELKTPQYVIDRINATDNCSYVEVRDLASVIGDLDVLYMTRVQKERFFNEDDYL
RLRDTYILDEEKLQLAKPSMAVLHPLPRVNEIAVDVDDDPRAAYFEQVKNGMLVRMALESTVVGDELPGYEPLNSKEVHA

Sequences:

>Translated_320_residues
MVGKSVVTLDGLSTNQILDLLHKAEYIDSHRKEVAHTCDGRVLATLFYEPSTRTRLSFETAMLRLGGKVIGFAGAQLASV
TKGESIADTLKTVSNYVDVVAIRHPKEGAALVASRAASVPVINAGDGGHMHPTQTLADLATLQSRFGRITDLTVGLCGDL
TFGRTVHSLIETLCRFGNVRFVLISPDELKTPQYVIDRINATDNCSYVEVRDLASVIGDLDVLYMTRVQKERFFNEDDYL
RLRDTYILDEEKLQLAKPSMAVLHPLPRVNEIAVDVDDDPRAAYFEQVKNGMLVRMALESTVVGDELPGYEPLNSKEVHA
>Mature_320_residues
MVGKSVVTLDGLSTNQILDLLHKAEYIDSHRKEVAHTCDGRVLATLFYEPSTRTRLSFETAMLRLGGKVIGFAGAQLASV
TKGESIADTLKTVSNYVDVVAIRHPKEGAALVASRAASVPVINAGDGGHMHPTQTLADLATLQSRFGRITDLTVGLCGDL
TFGRTVHSLIETLCRFGNVRFVLISPDELKTPQYVIDRINATDNCSYVEVRDLASVIGDLDVLYMTRVQKERFFNEDDYL
RLRDTYILDEEKLQLAKPSMAVLHPLPRVNEIAVDVDDDPRAAYFEQVKNGMLVRMALESTVVGDELPGYEPLNSKEVHA

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=306, Percent_Identity=42.483660130719, Blast_Score=241, Evalue=5e-64,
Organism=Homo sapiens, GI38788445, Length=318, Percent_Identity=25.7861635220126, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI2367364, Length=269, Percent_Identity=47.5836431226766, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI1790703, Length=267, Percent_Identity=29.2134831460674, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI193204318, Length=315, Percent_Identity=41.2698412698413, Blast_Score=224, Evalue=4e-59,
Organism=Saccharomyces cerevisiae, GI6322331, Length=274, Percent_Identity=44.1605839416058, Blast_Score=234, Evalue=9e-63,
Organism=Saccharomyces cerevisiae, GI6322373, Length=270, Percent_Identity=24.4444444444444, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24642586, Length=307, Percent_Identity=42.9967426710098, Blast_Score=246, Evalue=1e-65,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 35277; Mature: 35277

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGKSVVTLDGLSTNQILDLLHKAEYIDSHRKEVAHTCDGRVLATLFYEPSTRTRLSFET
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHH
AMLRLGGKVIGFAGAQLASVTKGESIADTLKTVSNYVDVVAIRHPKEGAALVASRAASVP
HHHHHCCEEEEECCCHHHHHCCCCHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHCCCC
VINAGDGGHMHPTQTLADLATLQSRFGRITDLTVGLCGDLTFGRTVHSLIETLCRFGNVR
EEECCCCCCCCHHHHHHHHHHHHHHCCCHHEEEEEHHCCCHHHHHHHHHHHHHHHHCCEE
FVLISPDELKTPQYVIDRINATDNCSYVEVRDLASVIGDLDVLYMTRVQKERFFNEDDYL
EEEECCCHHCCHHHHHHHCCCCCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCE
RLRDTYILDEEKLQLAKPSMAVLHPLPRVNEIAVDVDDDPRAAYFEQVKNGMLVRMALES
EEECEEEECHHHHHHCCCCHHHHCCCCCCCEEEEECCCCCHHHHHHHHHCCEEEEEEHHH
TVVGDELPGYEPLNSKEVHA
HHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVGKSVVTLDGLSTNQILDLLHKAEYIDSHRKEVAHTCDGRVLATLFYEPSTRTRLSFET
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHH
AMLRLGGKVIGFAGAQLASVTKGESIADTLKTVSNYVDVVAIRHPKEGAALVASRAASVP
HHHHHCCEEEEECCCHHHHHCCCCHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHCCCC
VINAGDGGHMHPTQTLADLATLQSRFGRITDLTVGLCGDLTFGRTVHSLIETLCRFGNVR
EEECCCCCCCCHHHHHHHHHHHHHHCCCHHEEEEEHHCCCHHHHHHHHHHHHHHHHCCEE
FVLISPDELKTPQYVIDRINATDNCSYVEVRDLASVIGDLDVLYMTRVQKERFFNEDDYL
EEEECCCHHCCHHHHHHHCCCCCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCE
RLRDTYILDEEKLQLAKPSMAVLHPLPRVNEIAVDVDDDPRAAYFEQVKNGMLVRMALES
EEECEEEECHHHHHHCCCCHHHHCCCCCCCEEEEECCCCCHHHHHHHHHCCEEEEEEHHH
TVVGDELPGYEPLNSKEVHA
HHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA