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The map label for this gene is insQ [H]

Identifier: 213692230

GI number: 213692230

Start: 1522180

End: 1523640

Strand: Direct

Name: insQ [H]

Synonym: Blon_1350

Alternate gene names: 213692230

Gene position: 1522180-1523640 (Clockwise)

Preceding gene: 213692229

Following gene: 213692237

Centisome position: 53.74

GC content: 59.82

Gene sequence:

>1461_bases
ATGAGCCAGAAGGTACGAATCATCAAAGTCAGGCATGCGGGCGCATCCGTGTACCTTGGCGACGATTCATGCCGCAACCA
TTGCTGGACGCGCAATCCCGAACGTATCATGGACTGGCTGTGCGATGGTTGGCGCACCCGTTTCAACCAGCATCGGGAGC
ATCGGACAATCCGCCGCTACATGGAGGACGTGGAATCCCGTGAGCGCATGTGGGTGGACGTTCCCTTGGGTGGGGCGACC
GTCGGGGAGCCGTTCAAGGACAGCGAGGCTCGAATCCGGTGTTCTTGGCTCGCATGCATTCCCTCCGCCGTTCTCGCCAG
TCCCATGCGCGTGGAGAATTCGGAATGGTATGCCGCGTTGAAGCGCAAGAAGATTAATGGCGGTCGTGTTCCGGGGTTCA
AATCCCGCAAACGCGCCCCCCAGTATTTCGTATGTTGGCGCAACCAGAACAAGACCGGCAACGCCGTCTACCATCAGGTG
TCACGCAAGCGTGGCGTGGTCGTAATCACCGGAAGCGTGAAAAAGGAATTCCGCAAGCCGGGCGAAACGGGATGCCGTTG
GATGCTCTCCATCCACGTGCGCGTCAGCCAACCCGTCAGGGATTACACGAGCGTGGCGGTGAATTGGACGGAACGCACTC
TGGCGTTCACCAACGAACCGTCGCCCATCCGACGGAATGCCGCCGGCAAGCAGACCGGTATCGACCGTGGCTGCGTCCAC
ACATTGGCCTTGTCGAATGGAACCATGCTGGACATGCCGCAACCGTCCGAACGGGAGAAGCGAGAGTATCTGCGCCTGCA
ACGCAAGCTCGCCCGACAAGACATGACCAACAGCCGACGTGGCGGGAAAACCGCGAAATTCCAGTCGAAACGACGCAAAC
TCACATTGAAGCGCATGAGTTCGATACGCCGCCGCATCAACAACCGCAAGGACGATTGGGTCGCGAAGACCACGACCCGA
CTGGTCGAAGACTACGACCTCATCGCCTTGGAAGCGTTGAACACCCGGCAGATGACCCGCAAGCCGAAACCGAAACAGGA
CCCCGACCATAAGGGACGCTACCTGCGCAACGGTTCGGCGGCCAAAGCGGGATTGAACCGCAGTATCCTCGGCAACCGTT
GGACGGACATCCAGGACAAACTCGAATACAAGACCCGTCTCGCCGGGACCCGGCTCATACTGGTCAACCCGGCGTACACG
TCCCAGACCTGCAACCGTTGCGGCCATGTCGCAAAGGAGAACCGTGAGAGCCAAGCGGTCTTCCACTGCGTCACCTGCGG
CAACAAGGACAATGCGGACATCAACGCGGCCAAGAACATCCTCGACCGCGCGATACACACAACCGGCATGGACGATGCCG
AGGGCGTGGAGGGACACGCTTCCCGTGAAACCTGTGTTTCACGGGAAGGTTCCGTTGAAACGCCAACCCCTGCCACGCGT
ACGGGAAGACCCCTCCAATGA

Upstream 100 bases:

>100_bases
GGGTCGGTGACCGGTTTGGCGGCGCAAGCCCCGTCTTTTAAGGCTGGGGTGAGCCGCCACTTCTTTGATTAATCACTGTT
TCCGTGGCAAGGTTTATAGC

Downstream 100 bases:

>100_bases
GTGAGGAACGTGTCTCACATGCGACAGGAATCCCCCGGCTTCAGCCGAGGGGAGGAAGTCAAGTGATATCCACGCTCAAC
CCCGATTCGACCGAACCGGA

Product: transposase, IS605 OrfB family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MSQKVRIIKVRHAGASVYLGDDSCRNHCWTRNPERIMDWLCDGWRTRFNQHREHRTIRRYMEDVESRERMWVDVPLGGAT
VGEPFKDSEARIRCSWLACIPSAVLASPMRVENSEWYAALKRKKINGGRVPGFKSRKRAPQYFVCWRNQNKTGNAVYHQV
SRKRGVVVITGSVKKEFRKPGETGCRWMLSIHVRVSQPVRDYTSVAVNWTERTLAFTNEPSPIRRNAAGKQTGIDRGCVH
TLALSNGTMLDMPQPSEREKREYLRLQRKLARQDMTNSRRGGKTAKFQSKRRKLTLKRMSSIRRRINNRKDDWVAKTTTR
LVEDYDLIALEALNTRQMTRKPKPKQDPDHKGRYLRNGSAAKAGLNRSILGNRWTDIQDKLEYKTRLAGTRLILVNPAYT
SQTCNRCGHVAKENRESQAVFHCVTCGNKDNADINAAKNILDRAIHTTGMDDAEGVEGHASRETCVSREGSVETPTPATR
TGRPLQ

Sequences:

>Translated_486_residues
MSQKVRIIKVRHAGASVYLGDDSCRNHCWTRNPERIMDWLCDGWRTRFNQHREHRTIRRYMEDVESRERMWVDVPLGGAT
VGEPFKDSEARIRCSWLACIPSAVLASPMRVENSEWYAALKRKKINGGRVPGFKSRKRAPQYFVCWRNQNKTGNAVYHQV
SRKRGVVVITGSVKKEFRKPGETGCRWMLSIHVRVSQPVRDYTSVAVNWTERTLAFTNEPSPIRRNAAGKQTGIDRGCVH
TLALSNGTMLDMPQPSEREKREYLRLQRKLARQDMTNSRRGGKTAKFQSKRRKLTLKRMSSIRRRINNRKDDWVAKTTTR
LVEDYDLIALEALNTRQMTRKPKPKQDPDHKGRYLRNGSAAKAGLNRSILGNRWTDIQDKLEYKTRLAGTRLILVNPAYT
SQTCNRCGHVAKENRESQAVFHCVTCGNKDNADINAAKNILDRAIHTTGMDDAEGVEGHASRETCVSREGSVETPTPATR
TGRPLQ
>Mature_485_residues
SQKVRIIKVRHAGASVYLGDDSCRNHCWTRNPERIMDWLCDGWRTRFNQHREHRTIRRYMEDVESRERMWVDVPLGGATV
GEPFKDSEARIRCSWLACIPSAVLASPMRVENSEWYAALKRKKINGGRVPGFKSRKRAPQYFVCWRNQNKTGNAVYHQVS
RKRGVVVITGSVKKEFRKPGETGCRWMLSIHVRVSQPVRDYTSVAVNWTERTLAFTNEPSPIRRNAAGKQTGIDRGCVHT
LALSNGTMLDMPQPSEREKREYLRLQRKLARQDMTNSRRGGKTAKFQSKRRKLTLKRMSSIRRRINNRKDDWVAKTTTRL
VEDYDLIALEALNTRQMTRKPKPKQDPDHKGRYLRNGSAAKAGLNRSILGNRWTDIQDKLEYKTRLAGTRLILVNPAYTS
QTCNRCGHVAKENRESQAVFHCVTCGNKDNADINAAKNILDRAIHTTGMDDAEGVEGHASRETCVSREGSVETPTPATRT
GRPLQ

Specific function: Required for the transposition of the insertion element (Potential) [H]

COG id: COG0675

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transposase 35 family [H]

Homologues:

Organism=Escherichia coli, GI288549314, Length=221, Percent_Identity=35.2941176470588, Blast_Score=124, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001959
- InterPro:   IPR010095
- InterPro:   IPR021027 [H]

Pfam domain/function: PF12323 HTH_14; PF01385 Transposase_2; PF07282 Transposase_35 [H]

EC number: NA

Molecular weight: Translated: 55658; Mature: 55527

Theoretical pI: Translated: 10.93; Mature: 10.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQKVRIIKVRHAGASVYLGDDSCRNHCWTRNPERIMDWLCDGWRTRFNQHREHRTIRRY
CCCCEEEEEEEECCCEEEECCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MEDVESRERMWVDVPLGGATVGEPFKDSEARIRCSWLACIPSAVLASPMRVENSEWYAAL
HHHHHHHCCEEEEECCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCEECCCHHHHHH
KRKKINGGRVPGFKSRKRAPQYFVCWRNQNKTGNAVYHQVSRKRGVVVITGSVKKEFRKP
HHHCCCCCCCCCCCHHCCCCCEEEEECCCCCCCHHHHHHHHHCCCEEEEECCHHHHHCCC
GETGCRWMLSIHVRVSQPVRDYTSVAVNWTERTLAFTNEPSPIRRNAAGKQTGIDRGCVH
CCCCCEEEEEEEEEECCCHHHHHHEEEEECCEEEEECCCCCCHHHCCCCCCCCCCHHHHE
TLALSNGTMLDMPQPSEREKREYLRLQRKLARQDMTNSRRGGKTAKFQSKRRKLTLKRMS
EEEECCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
SIRRRINNRKDDWVAKTTTRLVEDYDLIALEALNTRQMTRKPKPKQDPDHKGRYLRNGSA
HHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEHHCHHHHHCCCCCCCCCCCCCCEECCCCH
AKAGLNRSILGNRWTDIQDKLEYKTRLAGTRLILVNPAYTSQTCNRCGHVAKENRESQAV
HHHCCCHHHCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCEE
FHCVTCGNKDNADINAAKNILDRAIHTTGMDDAEGVEGHASRETCVSREGSVETPTPATR
EEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCC
TGRPLQ
CCCCCC
>Mature Secondary Structure 
SQKVRIIKVRHAGASVYLGDDSCRNHCWTRNPERIMDWLCDGWRTRFNQHREHRTIRRY
CCCEEEEEEEECCCEEEECCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MEDVESRERMWVDVPLGGATVGEPFKDSEARIRCSWLACIPSAVLASPMRVENSEWYAAL
HHHHHHHCCEEEEECCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCEECCCHHHHHH
KRKKINGGRVPGFKSRKRAPQYFVCWRNQNKTGNAVYHQVSRKRGVVVITGSVKKEFRKP
HHHCCCCCCCCCCCHHCCCCCEEEEECCCCCCCHHHHHHHHHCCCEEEEECCHHHHHCCC
GETGCRWMLSIHVRVSQPVRDYTSVAVNWTERTLAFTNEPSPIRRNAAGKQTGIDRGCVH
CCCCCEEEEEEEEEECCCHHHHHHEEEEECCEEEEECCCCCCHHHCCCCCCCCCCHHHHE
TLALSNGTMLDMPQPSEREKREYLRLQRKLARQDMTNSRRGGKTAKFQSKRRKLTLKRMS
EEEECCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
SIRRRINNRKDDWVAKTTTRLVEDYDLIALEALNTRQMTRKPKPKQDPDHKGRYLRNGSA
HHHHHHCCCCCHHHHHHHHHHHHCCCEEEEEHHCHHHHHCCCCCCCCCCCCCCEECCCCH
AKAGLNRSILGNRWTDIQDKLEYKTRLAGTRLILVNPAYTSQTCNRCGHVAKENRESQAV
HHHCCCHHHCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCEE
FHCVTCGNKDNADINAAKNILDRAIHTTGMDDAEGVEGHASRETCVSREGSVETPTPATR
EEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCC
TGRPLQ
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]