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The map label for this gene is ssoIIM [H]

Identifier: 213692102

GI number: 213692102

Start: 1403069

End: 1405711

Strand: Direct

Name: ssoIIM [H]

Synonym: Blon_1221

Alternate gene names: 213692102

Gene position: 1403069-1405711 (Clockwise)

Preceding gene: 213692101

Following gene: 213692103

Centisome position: 49.53

GC content: 68.07

Gene sequence:

>2643_bases
ATGCAGAACAATTCTGAACACAACGGTCTCATCGGCAGCCTCTTCTCCGGATACGGGGGATTGGATCTCGGCGTCGACAT
GGCGTTGGGCGGCGGCATGCGCGTCGCCTACACGAGCGACATCGAACCCGGCCCCTGCGCCATCGAGTCATATCACGCGC
ACGGCGACGACTGCCCGAACCTGGGCGACATCACCGGAATCGACTTCGAGAAGTTGCCCGATACGGACGTGGTCGTCGGC
GGGTCGCCCTGCCAGTCCCTGTCTCTTGCGGGTCTGCGCGCGGGCATGCATGAGGGCACCCGGTCGGGATTGTGGAGCTA
TCAGGCGGATATTCTGAAGGCTTCGAAGAGTCCCCTATTGCTATGGGAAAACGTCCAGGGTGCGTTGACCGCCACGGCCT
CCAGCCGCGAGGATCTGGCGGAGGCGGACCGGCGTGCGAAGGCTTTGGGGAAGGCGGGCCTGTGCGCGTGCGTGACGCCC
TCCGTCGACCCGCCGGACGGTTTCACGCCGCCCGACCCGGATGCGGAGGGGGCGAAGCCTTTGGCGACCGTCCTGCGCGA
TTATCTGGAAGCCGATCCGGAGAAGTGCGCGGCCGTCGTCTGCAAGGCGTGCGGCCGGCATGTGTTCGAAATCAACGGCG
ACACGGTGCTGGCGGATGGCGAGCGATTGGACGGCGTGCATACGGCGCCCACGATCCGCGCGTTGGGCCGTGTGCTGGGC
GATCTGGCGAACCTGGGTTATGACGCGGTGTGGCGAGGTCTTGAGGCCGCCGACATCGGGGCCCCTCACCATAGGCTGCG
CATCTTCGTGACCGCCTGGCCGCGCGACCCGTCCACGGCGCGCGAGCCGTCGAATGCCCGGCTGAGGCGTCTCGCCCAAC
TGCCGCCCATGAGGCCGAAGGGCCGCGCCTGGGCCGTATGGGATGCGGACAGGGACGTGTGGACGACAGGGGAGCCGGAT
TTGTTCGGCGATGTGGACGTGTATATGGACGCGTGGCCGAAATCGGGCGTCATGGCGGCGGGCCGCGTGTATATGGTGCC
CGGATCGTGGCTTACGGTCCCCGTGCCAGCGGCCGGATCGGCTTTGGTGACGCCGAAGGCGACGGACGGCGCGAAGGGCG
GGCCGAACCAGTCATACGGGAACGGGGATGCGCCGCTGCCCGCGCAGGCGGCCATGCTGCCGACCATCAAGGCGGGGGAT
GCGGGGCATACGGGGCCGAACCAGCGGTTCGGGGCCGGCAACCCGAGCCTGGCGGCCTCGGTCGCGGGAATCGGCGTCCT
TCCGACCCCGGCGGATGGGTTCCTGTACACGCCGAAGCAGTCGGACGGGGTGTTCGCCGCCCCGGCCACGTCGGGCCGCC
CGTTGGACAGGAGCACGTTCCTGTCCACGCAGGTGCGGCTCATGGACCTGCCGGGACGCATGGACCCGGAGAAGGTCAGG
GAGCATGATTCGAGGCTGCCGACCCAGATCGACCAGGCATCGTTCGCCTTGAGGCCGTCTGACGCATACCCGTGGTCATG
GACTGATGACGACCTGTGGCTGCTGACGCCGACGGTGATGGACGCGACCCGGAATACGGTGCGCGGCTGTTGGAGGCCGG
GGGAGAACTGGCGTGGCGTCTCGTTGAACAACGCGATCATGGACGCGGGCGAGGTGATCGCGGCGGGAACGCGTACGATC
CACCTGTCCGACGAGGTCACCTGCCTGCCGACGCCCACGTCGAACGAGTGGAACGGGGGCACGATGAGGCCCGCACGCCG
GGCCGTCATGGGGCATGCGGTCACGTGCGCCGACTGGGCGAAGAAGAGCGACGACATCCGCCTGTTCCCCACGCCGAACT
GCATGGATGCGATCATGCCCCGGTCTTCGACCGGCAATCTGAGGGAGGATGTGATGATGAGGAATGTGACCGTGCTGTCC
ACGCCGACGAGCAGAGGCGGGAAGGGCAGCAACCAGAGGAACAGTGAGGACTGCATGCCCGTGGCCGCCGACAACATGAG
CATCACCGTCCCCGGCCCCTATCATGGCGGCCTGTGGGGGTTGGACGACGGCAGGGGCGTGCGCTTCGGCCGGTTCACCG
CGGCGATCCGCCGTTGGGAGCGGGTGTCCGGCCGTCCCGCCCCCTGTCCCACGCAGGCGACCGGCGGCCTGCGCCGCTGG
ATCGGGGCGAACGCCGCCGACCCCACACTGTTCGAACCGTACTGGCTCGCCCGCCACGCCCCGTTCGACGCGCATCCGAA
AGCCCGGCTGGACCAGCCGATACGCGACCGCATGCTCGCCCGCTGGCGTGAAACCGACCGGGGGATGAGCCTCATCGACC
CCATGTTCTGGCCCGGCTGCAAGGACATGGACCCGGACATCCCGATCCCCGCGACCCGCCTGCCCGACAAAAGCGTGCTC
GATTATTGGAAGACCCGGTCCGTGGGCTCCCGGTTCCCGTCGGCCTCCCACCTATCCCCGAGGTTCGTGGAATGGATGAT
GGGTCTGGAGGACGGCTGGGTCACGAATCCGGCGATCTGGCGGCATGTGGACGGCAATCACAGGAACCTCCAACTGCGCG
CCCTGGGCAACGGGGTCGTGCCCCCGCAGGCCGCCATGGCGGTCGACTGGGCGCTCCGCGTACGCGAAAGGCTCGCCGTT
TGA

Upstream 100 bases:

>100_bases
GGAAACCATCACCATGCATGCGCTGACCCTCGAAGCCGACTCGTACATGGTGGACGGGATTCTCGTCAGGGCATGATGCG
GGGAACGCATACAGTCATGT

Downstream 100 bases:

>100_bases
CGCCCGTCAACGTCTGGGCGTGGACGCTCCTGGGCGTCGCCGTCCGCCCGGCCATACCCGCGTCCATGCGCCGGATCGGT
CTGATCCGGTCCGGCGCATG

Product: C-5 cytosine-specific DNA methylase

Products: NA

Alternate protein names: M.SsoII; Cytosine-specific methyltransferase SsoII [H]

Number of amino acids: Translated: 880; Mature: 880

Protein sequence:

>880_residues
MQNNSEHNGLIGSLFSGYGGLDLGVDMALGGGMRVAYTSDIEPGPCAIESYHAHGDDCPNLGDITGIDFEKLPDTDVVVG
GSPCQSLSLAGLRAGMHEGTRSGLWSYQADILKASKSPLLLWENVQGALTATASSREDLAEADRRAKALGKAGLCACVTP
SVDPPDGFTPPDPDAEGAKPLATVLRDYLEADPEKCAAVVCKACGRHVFEINGDTVLADGERLDGVHTAPTIRALGRVLG
DLANLGYDAVWRGLEAADIGAPHHRLRIFVTAWPRDPSTAREPSNARLRRLAQLPPMRPKGRAWAVWDADRDVWTTGEPD
LFGDVDVYMDAWPKSGVMAAGRVYMVPGSWLTVPVPAAGSALVTPKATDGAKGGPNQSYGNGDAPLPAQAAMLPTIKAGD
AGHTGPNQRFGAGNPSLAASVAGIGVLPTPADGFLYTPKQSDGVFAAPATSGRPLDRSTFLSTQVRLMDLPGRMDPEKVR
EHDSRLPTQIDQASFALRPSDAYPWSWTDDDLWLLTPTVMDATRNTVRGCWRPGENWRGVSLNNAIMDAGEVIAAGTRTI
HLSDEVTCLPTPTSNEWNGGTMRPARRAVMGHAVTCADWAKKSDDIRLFPTPNCMDAIMPRSSTGNLREDVMMRNVTVLS
TPTSRGGKGSNQRNSEDCMPVAADNMSITVPGPYHGGLWGLDDGRGVRFGRFTAAIRRWERVSGRPAPCPTQATGGLRRW
IGANAADPTLFEPYWLARHAPFDAHPKARLDQPIRDRMLARWRETDRGMSLIDPMFWPGCKDMDPDIPIPATRLPDKSVL
DYWKTRSVGSRFPSASHLSPRFVEWMMGLEDGWVTNPAIWRHVDGNHRNLQLRALGNGVVPPQAAMAVDWALRVRERLAV

Sequences:

>Translated_880_residues
MQNNSEHNGLIGSLFSGYGGLDLGVDMALGGGMRVAYTSDIEPGPCAIESYHAHGDDCPNLGDITGIDFEKLPDTDVVVG
GSPCQSLSLAGLRAGMHEGTRSGLWSYQADILKASKSPLLLWENVQGALTATASSREDLAEADRRAKALGKAGLCACVTP
SVDPPDGFTPPDPDAEGAKPLATVLRDYLEADPEKCAAVVCKACGRHVFEINGDTVLADGERLDGVHTAPTIRALGRVLG
DLANLGYDAVWRGLEAADIGAPHHRLRIFVTAWPRDPSTAREPSNARLRRLAQLPPMRPKGRAWAVWDADRDVWTTGEPD
LFGDVDVYMDAWPKSGVMAAGRVYMVPGSWLTVPVPAAGSALVTPKATDGAKGGPNQSYGNGDAPLPAQAAMLPTIKAGD
AGHTGPNQRFGAGNPSLAASVAGIGVLPTPADGFLYTPKQSDGVFAAPATSGRPLDRSTFLSTQVRLMDLPGRMDPEKVR
EHDSRLPTQIDQASFALRPSDAYPWSWTDDDLWLLTPTVMDATRNTVRGCWRPGENWRGVSLNNAIMDAGEVIAAGTRTI
HLSDEVTCLPTPTSNEWNGGTMRPARRAVMGHAVTCADWAKKSDDIRLFPTPNCMDAIMPRSSTGNLREDVMMRNVTVLS
TPTSRGGKGSNQRNSEDCMPVAADNMSITVPGPYHGGLWGLDDGRGVRFGRFTAAIRRWERVSGRPAPCPTQATGGLRRW
IGANAADPTLFEPYWLARHAPFDAHPKARLDQPIRDRMLARWRETDRGMSLIDPMFWPGCKDMDPDIPIPATRLPDKSVL
DYWKTRSVGSRFPSASHLSPRFVEWMMGLEDGWVTNPAIWRHVDGNHRNLQLRALGNGVVPPQAAMAVDWALRVRERLAV
>Mature_880_residues
MQNNSEHNGLIGSLFSGYGGLDLGVDMALGGGMRVAYTSDIEPGPCAIESYHAHGDDCPNLGDITGIDFEKLPDTDVVVG
GSPCQSLSLAGLRAGMHEGTRSGLWSYQADILKASKSPLLLWENVQGALTATASSREDLAEADRRAKALGKAGLCACVTP
SVDPPDGFTPPDPDAEGAKPLATVLRDYLEADPEKCAAVVCKACGRHVFEINGDTVLADGERLDGVHTAPTIRALGRVLG
DLANLGYDAVWRGLEAADIGAPHHRLRIFVTAWPRDPSTAREPSNARLRRLAQLPPMRPKGRAWAVWDADRDVWTTGEPD
LFGDVDVYMDAWPKSGVMAAGRVYMVPGSWLTVPVPAAGSALVTPKATDGAKGGPNQSYGNGDAPLPAQAAMLPTIKAGD
AGHTGPNQRFGAGNPSLAASVAGIGVLPTPADGFLYTPKQSDGVFAAPATSGRPLDRSTFLSTQVRLMDLPGRMDPEKVR
EHDSRLPTQIDQASFALRPSDAYPWSWTDDDLWLLTPTVMDATRNTVRGCWRPGENWRGVSLNNAIMDAGEVIAAGTRTI
HLSDEVTCLPTPTSNEWNGGTMRPARRAVMGHAVTCADWAKKSDDIRLFPTPNCMDAIMPRSSTGNLREDVMMRNVTVLS
TPTSRGGKGSNQRNSEDCMPVAADNMSITVPGPYHGGLWGLDDGRGVRFGRFTAAIRRWERVSGRPAPCPTQATGGLRRW
IGANAADPTLFEPYWLARHAPFDAHPKARLDQPIRDRMLARWRETDRGMSLIDPMFWPGCKDMDPDIPIPATRLPDKSVL
DYWKTRSVGSRFPSASHLSPRFVEWMMGLEDGWVTNPAIWRHVDGNHRNLQLRALGNGVVPPQAAMAVDWALRVRERLAV

Specific function: This methylase recognizes the double-stranded sequence CCNGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the SsoII endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525
- InterPro:   IPR001387
- InterPro:   IPR010982 [H]

Pfam domain/function: PF00145 DNA_methylase; PF01381 HTH_3 [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 95052; Mature: 95052

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00094 C5_MTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNNSEHNGLIGSLFSGYGGLDLGVDMALGGGMRVAYTSDIEPGPCAIESYHAHGDDCPN
CCCCCCCCCHHHHHHCCCCCCCCCEEEEECCCEEEEEECCCCCCCCEEECCCCCCCCCCC
LGDITGIDFEKLPDTDVVVGGSPCQSLSLAGLRAGMHEGTRSGLWSYQADILKASKSPLL
CCCCCCCCHHHCCCCCEEECCCCCCHHHHHHHHHHHHCCHHHCCHHHHHHHHHCCCCCEE
LWENVQGALTATASSREDLAEADRRAKALGKAGLCACVTPSVDPPDGFTPPDPDAEGAKP
EEECCCCEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCH
LATVLRDYLEADPEKCAAVVCKACGRHVFEINGDTVLADGERLDGVHTAPTIRALGRVLG
HHHHHHHHHHCCHHHHHHHHHHHCCCEEEEECCCEEEECCCCCCCCCCCHHHHHHHHHHH
DLANLGYDAVWRGLEAADIGAPHHRLRIFVTAWPRDPSTAREPSNARLRRLAQLPPMRPK
HHHHCCHHHHHCCCCHHHCCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCC
GRAWAVWDADRDVWTTGEPDLFGDVDVYMDAWPKSGVMAAGRVYMVPGSWLTVPVPAAGS
CCEEEEEECCCCEECCCCCCCCCCHHHEEECCCCCCCEECCEEEEECCCEEEEECCCCCC
ALVTPKATDGAKGGPNQSYGNGDAPLPAQAAMLPTIKAGDAGHTGPNQRFGAGNPSLAAS
EEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCEEECCCCCCCCCCCCCCCCCCHHHHH
VAGIGVLPTPADGFLYTPKQSDGVFAAPATSGRPLDRSTFLSTQVRLMDLPGRMDPEKVR
HHCCCCCCCCCCCEEECCCCCCCEEECCCCCCCCCCHHHHHHEEEEEEECCCCCCHHHHH
EHDSRLPTQIDQASFALRPSDAYPWSWTDDDLWLLTPTVMDATRNTVRGCWRPGENWRGV
HHHHCCCCHHCCCCEEECCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCCE
SLNNAIMDAGEVIAAGTRTIHLSDEVTCLPTPTSNEWNGGTMRPARRAVMGHAVTCADWA
ECCHHHHCCCCEEECCCEEEEECCCEEEECCCCCCCCCCCCCCHHHHHHHCCCEEHHHHH
KKSDDIRLFPTPNCMDAIMPRSSTGNLREDVMMRNVTVLSTPTSRGGKGSNQRNSEDCMP
CCCCCEEEECCCCHHHHHCCCCCCCCHHHHHHHHCEEEEECCCCCCCCCCCCCCCCCCCE
VAADNMSITVPGPYHGGLWGLDDGRGVRFGRFTAAIRRWERVSGRPAPCPTQATGGLRRW
EEECCEEEEECCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
IGANAADPTLFEPYWLARHAPFDAHPKARLDQPIRDRMLARWRETDRGMSLIDPMFWPGC
HCCCCCCCCCCCCHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHCCCCHHHCHHHCCCC
KDMDPDIPIPATRLPDKSVLDYWKTRSVGSRFPSASHLSPRFVEWMMGLEDGWVTNPAIW
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCHHE
RHVDGNHRNLQLRALGNGVVPPQAAMAVDWALRVRERLAV
EECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQNNSEHNGLIGSLFSGYGGLDLGVDMALGGGMRVAYTSDIEPGPCAIESYHAHGDDCPN
CCCCCCCCCHHHHHHCCCCCCCCCEEEEECCCEEEEEECCCCCCCCEEECCCCCCCCCCC
LGDITGIDFEKLPDTDVVVGGSPCQSLSLAGLRAGMHEGTRSGLWSYQADILKASKSPLL
CCCCCCCCHHHCCCCCEEECCCCCCHHHHHHHHHHHHCCHHHCCHHHHHHHHHCCCCCEE
LWENVQGALTATASSREDLAEADRRAKALGKAGLCACVTPSVDPPDGFTPPDPDAEGAKP
EEECCCCEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCH
LATVLRDYLEADPEKCAAVVCKACGRHVFEINGDTVLADGERLDGVHTAPTIRALGRVLG
HHHHHHHHHHCCHHHHHHHHHHHCCCEEEEECCCEEEECCCCCCCCCCCHHHHHHHHHHH
DLANLGYDAVWRGLEAADIGAPHHRLRIFVTAWPRDPSTAREPSNARLRRLAQLPPMRPK
HHHHCCHHHHHCCCCHHHCCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCC
GRAWAVWDADRDVWTTGEPDLFGDVDVYMDAWPKSGVMAAGRVYMVPGSWLTVPVPAAGS
CCEEEEEECCCCEECCCCCCCCCCHHHEEECCCCCCCEECCEEEEECCCEEEEECCCCCC
ALVTPKATDGAKGGPNQSYGNGDAPLPAQAAMLPTIKAGDAGHTGPNQRFGAGNPSLAAS
EEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCEEECCCCCCCCCCCCCCCCCCHHHHH
VAGIGVLPTPADGFLYTPKQSDGVFAAPATSGRPLDRSTFLSTQVRLMDLPGRMDPEKVR
HHCCCCCCCCCCCEEECCCCCCCEEECCCCCCCCCCHHHHHHEEEEEEECCCCCCHHHHH
EHDSRLPTQIDQASFALRPSDAYPWSWTDDDLWLLTPTVMDATRNTVRGCWRPGENWRGV
HHHHCCCCHHCCCCEEECCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCCE
SLNNAIMDAGEVIAAGTRTIHLSDEVTCLPTPTSNEWNGGTMRPARRAVMGHAVTCADWA
ECCHHHHCCCCEEECCCEEEEECCCEEEECCCCCCCCCCCCCCHHHHHHHCCCEEHHHHH
KKSDDIRLFPTPNCMDAIMPRSSTGNLREDVMMRNVTVLSTPTSRGGKGSNQRNSEDCMP
CCCCCEEEECCCCHHHHHCCCCCCCCHHHHHHHHCEEEEECCCCCCCCCCCCCCCCCCCE
VAADNMSITVPGPYHGGLWGLDDGRGVRFGRFTAAIRRWERVSGRPAPCPTQATGGLRRW
EEECCEEEEECCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
IGANAADPTLFEPYWLARHAPFDAHPKARLDQPIRDRMLARWRETDRGMSLIDPMFWPGC
HCCCCCCCCCCCCHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHCCCCHHHCHHHCCCC
KDMDPDIPIPATRLPDKSVLDYWKTRSVGSRFPSASHLSPRFVEWMMGLEDGWVTNPAIW
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCHHE
RHVDGNHRNLQLRALGNGVVPPQAAMAVDWALRVRERLAV
EECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7916706 [H]